Network representation of multicellular activity in pancreatic islets: Technical considerations for functional connectivity analysis

PLoS Computational Biology, Journal Year: 2024, Volume and Issue: 20(5), P. e1012130 - e1012130

Published: May 13, 2024

Within the islets of Langerhans, beta cells orchestrate synchronized insulin secretion, a pivotal aspect metabolic homeostasis. Despite inherent heterogeneity and multimodal activity individual cells, intercellular coupling acts as homogenizing force, enabling coordinated responses through propagation waves. Disruptions in this coordination are implicated irregular hallmark diabetes. Recently, innovative approaches, such integrating multicellular calcium imaging with network analysis, have emerged for quantitative assessment cellular islets. However, different groups use distinct experimental preparations, microscopic techniques, apply methods to process measured signals various derive functional connectivity patterns. This makes comparisons between findings their integration into bigger picture difficult has led disputes interpretations. To address these issues, we present here systematic analysis how approaches influence representation islet activity. Our show that choice used construct networks is not crucial, although care needed when combining data from Conversely, conclusions drawn can be heavily affected by pre-processing time series, type oscillatory component signals, preparation. tutorial-like investigation aims resolve interpretational reconcile conflicting views, advance implications, encourage researchers adopt analysis. As conclude, outline challenges future research, emphasizing broader applicability our other tissues exhibiting complex dynamics.

Language: Английский

Both electrical and metabolic coupling shape the collective multimodal activity and functional connectivity patterns in beta cell collectives: A computational model perspective

Physical review. E, Journal Year: 2023, Volume and Issue: 108(5)

Published: Nov. 22, 2023

Pancreatic beta cells are coupled excitable oscillators that synchronize their activity via different communication pathways. Their oscillatory manifests itself on multiple timescales and consists of bursting electrical activity, subsequent oscillations in the intracellular ${\mathrm{Ca}}^{2+}$, as well metabolism exocytosis. The coordination intricate multicellular level plays a key role regulation physiological pulsatile insulin secretion is incompletely understood. In this paper, we investigate theoretically principles give rise to synchronized cell populations by building up phenomenological model incorporates basic features dynamics. Specifically, composed slow fast units reflect metabolic processes respectively. Using realistic description intercellular interactions, study how combination coupling generates collective rhythmicity shapes functional networks. It turns out while solely can responses, addition interactions further enhances coordination, spatial range increases number connections networks, ensures better consistency with experimental findings. Moreover, our computational results provide additional insights into relationship between heterogeneity, profiles, connectivity, supplementing thereby recent endocrine

Language: Английский