Common salt (NaCl) causes developmental, behavioral, and physiological defects in Drosophila melanogaster

Nutritional Neuroscience, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19

Published: Jan. 6, 2025

Purpose The incidence of obesity has surged to pandemic levels in recent decades. Approximately 1.89 million are linked excessive salt consumption. This study aims check the toxicity at different concentrations using an invertebrate model organism Drosophila melanogaster.

Language: Английский

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Heterogeneous expression of dopaminergic markers and Vglut2 in mouse mesodiencephalic dopaminergic nuclei A8–A13

The Journal of Comparative Neurology, Journal Year: 2020, Volume and Issue: 529(7), P. 1273 - 1292

Published: Sept. 1, 2020

Co-transmission of glutamate by brain dopaminergic (DA) neurons was recently proposed as a potential factor influencing cell survival in models Parkinson's disease. Intriguingly, DA nuclei are differentially affected Whether this is associated with different patterns co-expression the glutamatergic phenotype along rostrocaudal axis unknown mammals. We hypothesized that, zebrafish, present preferentially caudal mesodiencephalic nuclei. Here, we used mice fate mapping strategy based on reporter protein expression (ZsGreen) consecutive to previous vesicular transporter 2 (Vglut2) gene, coupled immunofluorescence experiments against tyrosine hydroxylase (TH) or dopamine (DAT). found three cells, organized axis. The first pattern (TH-positive, DAT-positive, ZsGreen-positive) A8-A10. second DAT-negative, A11. third ZsGreen-negative) A12-A13. These should help refine establishment homology between vertebrate species. Our results also uncover that Vglut2 expressed at some point during lifetime known degenerate disease and largely absent from those preserved, suggesting cells influences their

Language: Английский

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An inhibitory mechanism for suppressing high salt intake in Drosophila

Chemical Senses, Journal Year: 2023, Volume and Issue: 48

Published: Jan. 1, 2023

Abstract High concentrations of dietary salt are harmful to health. Like most animals, Drosophila melanogaster attracted foods that have low salt, but show strong taste avoidance high foods. Salt in known on multiple classes neurons, activating Gr64f sweet-sensing neurons drive food acceptance and 2 others (Gr66a bitter Ppk23 salt) rejection. Here we find NaCl elicits a bimodal dose-dependent response which activity with depressed salt. also inhibits the sugar this action is independent neuron’s Consistent electrophysiological analysis, feeding suppression presence correlates inhibition neuron activity, remains if genetically silenced. Other salts such as Na2SO4, KCl, MgSO4, CaCl2, FeCl3 act behavior same way. A comparison effects various suggests dictated by cationic moiety rather than anionic component Notably, salt-dependent not observed Gr66a neurons—response canonical tastant, denatonium, altered Overall, study characterizes mechanism appetitive can deter ingestion potentially salts.

Language: Английский

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Dietary Approaches to Stop Hypertension (DASH) Score and Its Association with Sleep Quality in a National Survey of Middle-Aged and Older Men and Women

Nutrients, Journal Year: 2020, Volume and Issue: 12(5), P. 1510 - 1510

Published: May 22, 2020

Complex processes appear to link sleep duration and quality with dietary patterns. Numerous studies show healthful benefits of the Dietary Approaches Stop Hypertension (DASH) diet, but few have examined its association or quality. The current study tested cross-sectional associations DASH diet score among adults. Analyses participants were from 2005–2008 wave National Health Nutrition Examination Surveys (n = 3941 adults ≥30 years age, complete data). We performed sex- age group-stratified multiple OLS regression analyses total components as main predictors outcomes, adjusting sequentially for socio-demographic, behavioral health-related factors. Sex differences in quality, covariates, also by incorporating two-way interaction terms between sex/age each unstratified model. found that was inversely related poor sleep-related daytime dysfunction adjusted sex, demographic socio-economic Some sex-specific detected component scores Notably, potassium associated Factor 1 (“sleepiness disturbance”) women. fiber better 2 (“sleep-related dysfunction”) younger subjects. This indicates health Future longitudinal randomized placebo-controlled trials are required ascertain protective effects.

Language: Английский

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Preference and detrimental effects of high fat, sugar, and salt diet in wild‐caught Drosophila simulans are reversed by flight exercise

FASEB BioAdvances, Journal Year: 2020, Volume and Issue: 3(1), P. 49 - 64

Published: Oct. 28, 2020

High saturated fat, sugar, and salt contents are a staple of Western diet (WD), contributing to obesity, metabolic syndrome, plethora other health risks. However, the combinatorial effects these ingredients have not been fully evaluated. Here, using wild-caught Drosophila simulans, we show that enriched with is more detrimental than each ingredient separately, resulting in significantly decreased lifespan, locomotor activity, sleep, reproductive function, mitochondrial function. These were pronounced female male flies. Adding regular flight exercise flies on WD markedly negated adverse WD. At molecular level, increased levels triglycerides caused dysfunction, while counterbalanced effects. Interestingly, fruit developed preference for after pre-exposure, which was averted by exercise. The results demonstrate aerobic can mitigate dietary fly physiology, feeding behavior. Our data establish simulans as novel model diet-exercise interaction bears strong similarity pathophysiology obesity eating disorders humans.

Language: Английский

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Artificial diet alters activity and rest patterns in the olive fruit fly

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(2), P. e0274586 - e0274586

Published: Feb. 21, 2023

Olive fruit flies, Bactrocera oleae (Diptera: Tephritidae) reared in the laboratory on an artificial diet are essential for genetic control techniques against this pest. However, colony’s adaptation can affect quality of flies. We used Locomotor Activity Monitor to track activity and rest patterns adult olive flies as immatures olives (F2-F3 generation) (>300 generations). Counts beam breaks caused by fly were estimation its locomotor levels during light dark period. Bouts inactivity with duration longer than five minutes considered a episode. parameters found be dependent sex, mating status rearing history. In virgin olives, males more active females increased their towards end Mating decreased males, but not female olive-reared Laboratory had lower period episodes shorter compared olives. describe diurnal B . adults diet. discuss how pattern differences may flies’ ability compete wild field.

Language: Английский

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Role of muscle FOXO gene in exercise against the skeletal muscle and cardiac age-related defects and mortality caused by high-salt intake in Drosophila

Genes & Nutrition, Journal Year: 2023, Volume and Issue: 18(1)

Published: March 30, 2023

FOXO has long been associated with aging, exercise, and tissue homeostasis, but it remains unclear what the role is of muscle gene in E against high-salt intake(HSI)-induced age-related defects skeletal muscle, heart, mortality. In this research, overexpression RNAi heart Drosophila were constructed by building Mhc-GAL4/FOXO-UAS-overexpression Mhc-GAL4/FOXO-UAS-RNAi system. The function, balance oxidation antioxidant, mitochondrial homeostasis measured. results showed that exercise reversed decline climbing ability downregulation expression induced HSI. Muscle-specific FOXO-RNAi (FOXO-RNAi) -overexpression (FOXO-OE) promoted or slowed ability, structure damage, which was accompanied inhibition activation FOXO/PGC-1α/SDH FOXO/SOD pathway activity, oxidative stress (ROS) increased decreased both heart. protective effect on blocked aged HSI flies. FOXO-OE prolonged its lifespan, did not resist HSI-induced lifespan shortening. Exercise improve shortening Therefore, current confirmed played a vital because determined activity pathways. also an important mortality aging

Language: Английский

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The prominin-like Gene Expressed in a Subset of Dopaminergic Neurons Regulates Locomotion in Drosophila

Molecules and Cells, Journal Year: 2022, Volume and Issue: 45(9), P. 640 - 648

Published: Aug. 24, 2022

CD133, also known as prominin-1, was first identified a biomarker of mammalian cancer and neural stem cells. Previous studies have shown that the

Language: Английский

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Expression of circadian clock genes in leukocytes of patients with Meniere's disease

Laryngoscope Investigative Otolaryngology, Journal Year: 2022, Volume and Issue: 7(2), P. 584 - 591

Published: Feb. 18, 2022

The underlying etiology of Meniere's disease (MD) is not completely clear, but the precipitated triggers may alter circadian clock in patients with MD. This study aims to survey expression genes peripheral blood (PB) leukocytes MD patients.We investigated nine PB and normal controls using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).We observed significantly lower PER1 gene higher CLOCK than those (p < 0.05). did associate degree dizziness handicap MD, a was correlated pure tone average (PTA) speech reception threshold affected ear Patients PTA > 30 dB had ≤30 Our qRT-PCR result validated by fewer positively stained for protein immunocytochemical study.Our implies alteration In particular, downregulation hearing loss ear. be potential marker progression

Language: Английский

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High-Salt Diet Impairs the Neurons Plasticity and the Neurotransmitters-Related Biological Processes

Nutrients, Journal Year: 2021, Volume and Issue: 13(11), P. 4123 - 4123

Published: Nov. 17, 2021

Salt, commonly known as sodium chloride, is an important ingredient that the body requires in relatively minute quantities. However, consuming too much salt can lead to high blood pressure, heart disease and even disruption of circadian rhythms. The biological process rhythm was first studied Drosophila melanogaster well understood. Their locomotor activity gradually increases before light switched on off, a phenomenon called anticipation. In previous study, we showed high-salt diet (HSD) impairs morning anticipation behavior Drosophila. Here, found HSD did not significantly disrupt clock gene oscillation heads flies, nor it PERIOD protein neurons or peripheral tissues. Remarkably, neuronal plasticity axonal projections pacemaker neurons. Interestingly, increased excitability PDF mimics HSD, which causes impairment. Moreover, disrupts neurotransmitter-related processes brain. Taken together, our data show affects multiple functions physiological behaviors.

Language: Английский

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