Transplantation
of
neural
stem
cells
(NSCs)
has
been
proved
to
promote
functional
rehabilitation
brain
lesions
including
ischemic
stroke.
However,
the
therapeutic
effects
NSC
transplantation
are
limited
by
low
survival
and
differentiation
rates
NSCs
due
harsh
environment
in
after
Here,
we
employed
derived
from
human
induced
pluripotent
together
with
exosomes
extracted
treat
cerebral
ischemia
middle
artery
occlusion/reperfusion
mice.
The
results
showed
that
NSC-derived
significantly
reduced
inflammatory
response,
alleviated
oxidative
stress
transplantation,
facilitated
vivo.
combination
ameliorated
injury
tissue
infarction,
neuronal
death,
glial
scarring,
promoted
recovery
motor
function.
To
explore
underlying
mechanisms,
analyzed
miRNA
profiles
potential
downstream
genes.
Our
study
provided
rationale
for
clinical
application
as
a
supportive
adjuvant
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(3), P. 378 - 378
Published: March 20, 2024
Ischemic
stroke
is
a
fatal
and
disabling
disease
worldwide
imposes
significant
burden
on
society.
At
present,
biological
markers
that
can
be
conveniently
measured
in
body
fluids
are
lacking
for
the
diagnosis
of
ischemic
stroke,
there
no
effective
treatment
methods
to
improve
neurological
function
after
stroke.
Therefore,
new
ways
diagnosing
treating
urgently
needed.
The
neurovascular
unit,
composed
neurons,
astrocytes,
microglia,
other
components,
plays
crucial
role
onset
progression
Extracellular
vesicles
nanoscale
lipid
bilayer
secreted
by
various
cells.
key
extracellular
vesicles,
which
released
cells
unit
serve
as
facilitators
cellular
communication,
has
been
extensively
documented
recent
literature.
Here,
we
highlight
unit-derived
current
status
vesicle
engineering
treatment,
problems
encountered
clinical
translation
therapies.
derived
from
could
provide
an
important
contribution
diagnostic
therapeutic
tools
future,
more
studies
this
area
should
carried
out.
Journal of Translational Medicine,
Journal Year:
2022,
Volume and Issue:
20(1)
Published: Sept. 16, 2022
Currently,
there
is
no
regenerative
therapy
for
patients
with
neurological
and
neurodegenerative
disorders.
Cell-therapies
have
emerged
as
a
potential
treatment
numerous
brain
diseases.
Despite
recent
advances
in
stem
cell
technology,
major
concerns
been
raised
regarding
the
feasibility
safety
of
therapies
clinical
applications.We
generated
good
manufacturing
practice
(GMP)-compatible
neural
progenitor
cells
(NPCs)
from
transgene-
xeno-free
induced
pluripotent
(iPSCs)
that
can
be
smoothly
adapted
applications.
NPCs
were
characterized
vitro
their
differentiation
vivo
after
transplantation
into
wild
type
well
genetically
immunosuppressed
mice.Generated
had
stable
gene-expression
over
at
least
15
passages
could
scaled
up
to
1018
per
initially
seeded
106
cells.
After
withdrawal
growth
factors
vitro,
fate
mainly
differentiated
active
neurons.
To
ensure
pure
NPC
population
applications,
we
reduced
risk
iPSC
contamination
by
applying
micro
RNA-switch
technology
checkpoint.
Using
lentiviral
transduction
fluorescent
bioluminescent
dual-reporter
construct,
combined
non-invasive
imaging,
longitudinally
tracked
grafted
healthy
wild-type
mice
mouse
model
ischemic
stroke.
Long
term
in-depth
characterization
revealed
transplanted
capability
survive
spontaneously
differentiate
functional
mature
neurons
throughout
time
course
month,
while
residual
detectable.We
describe
generation
NPCs.
This
simple
protocol
ability
tracking
presents
valuable
tool
develop
safe
effective
various
injuries.
Journal of Nanobiotechnology,
Journal Year:
2022,
Volume and Issue:
20(1)
Published: Aug. 31, 2022
Ischemic
stroke
is
one
of
the
most
serious
global
public
health
problems.
However,
performance
current
therapeutic
regimens
limited
due
to
their
poor
target
specificity,
narrow
time
window,
and
compromised
effect.
To
overcome
these
barriers,
we
designed
an
ischemia-homing
bioengineered
nano-scavenger
by
camouflaging
a
catalase
(CAT)-loaded
self-assembled
tannic
acid
(TA)
nanoparticle
with
M2-type
microglia
membrane
(TPC@M2
NPs)
for
ischemic
treatment.
Journal of Personalized Medicine,
Journal Year:
2022,
Volume and Issue:
12(9), P. 1485 - 1485
Published: Sept. 10, 2022
In
the
past,
several
animal
disease
models
were
developed
to
study
molecular
mechanism
of
neurological
diseases
and
discover
new
therapies,
but
lack
equivalent
has
minimized
success
rate.
A
number
critical
issues
remain
unresolved,
such
as
high
costs
for
developing
models,
ethical
issues,
resemblance
with
human
disease.
Due
poor
initial
screening
assessment
molecules,
more
than
90%
drugs
fail
during
final
step
clinical
trial.
To
overcome
these
limitations,
a
approach
been
based
on
induced
pluripotent
stem
cells
(iPSCs).
The
discovery
iPSCs
provided
roadmap
translation
research
regeneration
therapy.
this
article,
we
discuss
potential
role
patient-derived
in
their
contribution
scientific
future
medicine.
humaniPSCs
most
common
neurodegenerative
(e.g.,
Parkinson's
Alzheimer's
disease,
diabetic
neuropathy,
stroke,
spinal
cord
injury)
examined
ranked
per
published
literature
PUBMED.
We
have
observed
that
scored
highest,
followed
by
Furthermore,
also
explored
recent
advancements
field
personalized
medicine,
patient-on-a-chip
concept,
where
can
be
grown
3D
matrices
inside
microfluidic
devices
create
an
vitro
model
Frontiers in Pharmacology,
Journal Year:
2023,
Volume and Issue:
14
Published: March 3, 2023
Background:
Stroke
is
one
of
the
leading
causes
mortality
and
permanent
disability
worldwide.
However,
current
stroke
treatment
has
a
limited
effect.
Therefore,
new
urgently
needed.
Stem
cell
therapy
cutting-edge
for
patients.
This
study
aimed
to
gain
better
understanding
global
stem
trends
in
via
bibliometric
analysis.
Methods:
We
used
Web
Science
Core
Collection
search
pertinent
articles
about
cells
published
between
2004
2022.
Analysis
was
conducted
using
CiteSpace,
VOSviewer,
R
package
“bibliometrix”
identify
publication
outputs,
countries/regions,
institutions,
authors/co-cited
authors,
journals/co-cited
journals,
co-cited
references,
keywords.
Results:
A
total
6,703
publications
were
included
The
number
citations
significantly
rapidly
increased
2022,
with
most
pronounced
growth
pattern
observed
period
2008–2009.
In
terms
authoritarian
countries,
USA
had
among
countries.
As
institutions
prolific
institution
University
South
Florida,
followed
by
Oakland
then
Shanghai
Jiao
Tong
University,
Chopp,
M.
Borlongan,
Cesario
V,
output
authors.
Regarding
Cell
Transplantation
highest
publication,
Brain
Research
.
“Mesenchymal
as
trophic
mediators”
frequently
cited
(2,082),
article
entitled
Neuronal
replacement
from
endogenous
precursors
adult
brain
after
strongest
burstiness
(strength
=
81.35).
Emerging
hot
words
past
decade
“adhesion
molecule,”
“mesenchymal
stromal
cell,”
“extracellular
vesicle,”
“pluripotent
cells,”
“signaling
pathway,”
“plasticity,”
“exosomes.”
Conclusion:
Between
“neurogenesis,”
“angiogenesis,”
“exosomes,”
“inflammation,”
“oxidative
stress”
have
emerged
research
areas
on
stroke.
Although
exert
positive
effects,
main
mechanisms
mitigating
damage
caused
are
still
unknown.
Clinical
challenges
may
include
complicating
factors
that
can
affect
efficacy
therapy,
which
worth
deep
exploration.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 10, 2025
Abstract
Globally,
there
are
15
million
stroke
patients
each
year
who
have
significant
neurological
deficits.
Today,
no
treatments
that
directly
address
these
With
demographics
shifting
to
an
older
population,
the
problem
is
worsening.
Therefore,
it
crucial
develop
feasible
therapeutic
for
stroke.
In
this
study,
we
tested
exosomes
derived
from
embryonic
endothelial
progenitor
cells
(eEPC)
assess
their
efficacy
in
a
rat
model
of
ischemic
Importantly,
developed
purification
methods
aimed
at
producing
robust
and
scalable
suitable
manufacturing
clinical
grade
exosomes.
We
characterized
exosome
cargos
including
RNA-seq,
miRNAs
targets,
proteomic
mass
spectrometry
analysis,
found
eEPC-exosomes
were
enhanced
with
angiogenic
(i.e.,
miR-126),
anti-inflammatory
miRNA
miR-146),
anti-apoptotic
miR-21).
The
activity
diverse
sourced
panel
eEPC
production
lines
was
assessed
vitro
by
live-cell
vascular
tube
formation
scratch
wound
assays,
showing
several
promoted
proliferation,
formation,
migration
cells.
further
applied
systemically
middle
cerebral
artery
occlusion
(MCAO)
recovery
(mNSS)
after
injury
animals.
mNSS
scores
revealed
sensorimotor
functioning
MCAO
rats
increased
significantly
intravenous
administration
outpaced
obtained
through
treatment
umbilical
cord
stem
Finally,
investigated
potential
mechanism
mitigating
inflammation
expression
neuronal,
endothelial,
inflammatory
markers.
Taken
together,
data
support
finding
eEPCs
provide
valuable
source
developing
products
therapies
other
diseases.
MedComm,
Journal Year:
2025,
Volume and Issue:
6(5)
Published: April 24, 2025
ABSTRACT
From
the
pioneering
days
of
cell
therapy
to
achievement
bioprinting
organs,
tissue
engineering,
and
regenerative
medicine
have
seen
tremendous
technological
advancements,
offering
solutions
for
restoring
damaged
tissues
organs.
However,
only
a
few
products
technologies
received
United
States
Food
Drug
Administration
approval.
This
review
highlights
significant
progress
in
therapy,
extracellular
vesicle‐based
engineering.
Hematopoietic
stem
transplantation
is
powerful
tool
treating
many
diseases,
especially
hematological
malignancies.
Mesenchymal
cells
been
extensively
studied.
The
discovery
induced
pluripotent
has
revolutionized
disease
modeling
applications,
paving
way
personalized
medicine.
Gene
represents
an
innovative
approach
treatment
genetic
disorders.
Additionally,
therapies
emerged
as
rising
stars,
promising
diagnostics,
cell‐free
therapeutics,
drug
delivery,
targeted
therapy.
Advances
engineering
enable
complex
constructs,
further
transforming
field.
Despite
these
technical,
ethical,
regulatory
challenges
remain.
addresses
current
bottlenecks,
emphasizing
novel
interdisciplinary
research
overcome
hurdles.
Standardizing
practices
conducting
clinical
trials
will
balance
innovation
regulation,
improving
patient
outcomes
quality
life.
Exploration of Neuroprotective Therapy,
Journal Year:
2024,
Volume and Issue:
unknown, P. 172 - 197
Published: April 26, 2024
Stroke,
one
of
the
leading
causes
global
morbidity
and
mortality,
results
from
disrupted
cerebral
blood
circulation,
leads
to
cellular
damage
or
death.
Ischemic
stroke,
predominant
subtype,
relies
mainly
on
recombinant
tissue
plasminogen
activator
(rtPA)
endovascular
thrombectomy
for
treatment.
Neurological
impairments
following
ischemic
stroke
highlight
importance
understanding
interplay
between
neuroinflammation
neurogenesis
in
brain
repair.
Research
reveals
a
complex
relationship,
where
inflammation
both
promotes
hinders
neurogenesis,
impacting
post-stroke
outcomes.
The
subventricular
zone
(SVZ)
striatum
sub
granular
(SGZ)
hippocampus
play
pivotal
roles
adult
with
distinct
characteristics
functions.
SVZ
involves
neuroblast
progenitors
migrating
olfactory
bulb,
while
SGZ
facilitates
granule
cell
generation
hippocampal
function.
Understanding
intricate
processes
neuroinflammation,
angiogenesis
is
crucial
developing
effective
therapeutics.
Promising
avenues
include
drug
therapy,
selective
serotonin
reuptake
inhibitors,
antibody
stimulation,
growth
factor
hormone
miRNAs,
extracellular
vesicles,
neuroprotective
agents.
Stem
exploring
various
types,
holds
potential
neuronal
replacement
recovery.
In
conclusion,
deciphering
unraveling
complexity
neuroinflammation’s
impact
repair,
diverse
therapeutic
approaches
need
comprehensive
investigations
enhance
multifaceted
landscape
therapeutics
presents
challenges,
but
ongoing
research
offers
promising
bridging
gap
preclinical
findings
clinical
treatments.
Asian Journal of Pharmaceutical Sciences,
Journal Year:
2023,
Volume and Issue:
19(1), P. 100867 - 100867
Published: Nov. 10, 2023
Ischemic
stroke
(IS)
causes
severe
disability
and
high
mortality
worldwide.
Stem
cell
(SC)
therapy
exhibits
unique
therapeutic
potential
for
IS
that
differs
from
current
treatments.
SC's
homing,
differentiation
paracrine
abilities
give
hope
neuroprotection.
Recent
studies
on
SC
modification
have
enhanced
effects
IS,
including
gene
transfection,
nanoparticle
modification,
biomaterial
pretreatment.
These
methods
improve
survival
rate,
neural
differentiation,
in
ischemic
areas.
However,
many
problems
must
be
resolved
before
can
clinically
applied.
issues
include
production
quality
quantity,
stability
during
transportation
storage,
as
well
usage
regulations.
Herein,
we
reviewed
the
brief
pathogenesis
of
"multi-mechanism"
advantages
SCs
treating
various
methods,
challenges.
We
aim
to
uncover
overcome
challenges
using
convey
innovative
ideas
modifying
SCs.
