N-Acetylcysteine-Amide Protects Against Acute Acrylamide Neurotoxicity in Adult Zebrafish DOI Creative Commons

Niki Tagkalidou,

Júlia Goyenechea, Irene Romero-Alfano

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(5), P. 362 - 362

Published: April 30, 2025

Acrylamide (ACR) is a potent neurotoxicant that disrupts cellular redox homeostasis by depleting reduced glutathione (GSH) and inducing oxidative stress. Despite its well-characterized mechanism, no effective treatments for ACR-induced neurotoxicity currently exist. This study evaluates the therapeutic efficacy of N-acetylcysteine-amide (AD4), blood–brain barrier (BBB)-permeable derivative N-acetylcysteine, in novel severe acute ACR model adult zebrafish. Adult zebrafish received single intraperitoneal (i.p.) injection (800 μg/g), followed AD4 (400 μg/g i.p.) or PBS 24 h later. exposure brain GSH levels 51% reduction at 48 h, an effect fully reversed treatment. Behavioral analyses showed rescued deficits short-term habituation acoustic startle response (ASR). Surprisingly, did not alter neurochemical profile key neurotransmitters expression genes related to homeostasis, synaptic vesicle recycling, regeneration, myelination. These results demonstrate AD4’s neuroprotective effects against toxicity, highlighting potential validating as translational studying neurotoxic mechanisms interventions.

Language: Английский

Pharmacological properties, molecular mechanisms and therapeutic potential of ginsenoside Rg3 as an antioxidant and anti-inflammatory agent DOI Creative Commons
Jing Wang, Li Zeng, Ying Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 5, 2022

Inflammation and oxidative stress lead to various acute or chronic diseases, including pneumonia, liver kidney injury, cardiovascular cerebrovascular metabolic cancer. Ginseng is a well-known widely used ethnic medicine in Asian countries, ginsenoside Rg3 saponin isolated from Panax ginseng C. A. Meyer, notoginseng, quinquefolius L. This compound has wide range of pharmacological properties, antioxidant anti-inflammatory activities, which have been evaluated disease models inflammation stress. can attenuate lung inflammation, prevent function damage, mitigate neuroinflammation, cerebral myocardial ischemia–reperfusion improve hypertension diabetes symptoms. The multitarget, multipathway mechanisms action gradually deciphered. review summarizes the existing knowledge on effects underlying molecular Rg3, suggesting that may be promising candidate drug for treatment diseases with inflammatory conditions.

Language: Английский

Citations

50
Acrylamide in food: Occurrence, metabolism, molecular toxicity mechanism and detoxification by phytochemicals DOI
Fangfang Yan, Li Wang, Li Zhao

et al.

Food and Chemical Toxicology, Journal Year: 2023, Volume and Issue: 175, P. 113696 - 113696

Published: March 2, 2023

Language: Английский

Citations

36
Ferroptosis implication in environmental-induced neurotoxicity DOI
Yiping Zhang,

Jun Xie

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 934, P. 172618 - 172618

Published: April 24, 2024

Language: Английский

Citations

11
Vat Photopolymerization Based Digital Light Processing 3D Printing Hydrogels in Biomedical Fields: Key Parameters and Perspective DOI
Zhe Lü,

Weizi Gao,

Fukang Liu

et al.

Additive manufacturing, Journal Year: 2024, Volume and Issue: unknown, P. 104443 - 104443

Published: Sept. 1, 2024

Language: Английский

Citations

10
Ultra-Processed Food Consumption and Depressive Symptoms in a Mediterranean Cohort DOI Open Access
Justyna Godos, Marialaura Bonaccio,

Wahidah H. Al-Qahtani

et al.

Nutrients, Journal Year: 2023, Volume and Issue: 15(3), P. 504 - 504

Published: Jan. 18, 2023

Excess consumption of ultra-processed foods (UPFs) is currently under investigation for its potentially detrimental impact on human health. Current evidence demonstrates a substantial association with an increased risk metabolic disorders, but data mental health outcomes are just emerging. The aim this study was to investigate the relationship between UPFs and depressive symptoms in sample younger Italian adults. A cross-sectional conducted 596 individuals (age 18-35 y) recruited southern Italy. Food frequency questionnaires NOVA classification were used assess dietary factors; Center Epidemiological Studies Depression Short Form (CES-D-10) presence symptoms. Individuals highest quartile UPF had higher odds having energy-adjusted model (odds ratio (OR) = 1.89, 95% confidence interval (CI): 1.06, 3.28); remained significant after adjusting potential confounding factors (OR 2.04, CI: 1.04, 4.01) became even stronger further adjustment adherence Mediterranean diet as proxy quality 2.70, 1.32, 5.51). In conclusion, positive likelihood found individuals. Given consistency findings quality, studies needed understand whether non-nutritional may play role neurobiology.

Language: Английский

Citations

22
Evaluation of multi-walled carbon nanotubes bearing aldehyde groups of different lengths for the immobilization of Geobacillus kaustophilus l-asparaginase DOI
İnci Özdemir, Ahmet Tülek, Burcu Karaaslan

et al.

Molecular Catalysis, Journal Year: 2024, Volume and Issue: 555, P. 113903 - 113903

Published: Feb. 1, 2024

Language: Английский

Citations

7
The Optimization Design of Macrophage Membrane Camouflaging Liposomes for Alleviating Ischemic Stroke Injury through Intranasal Delivery DOI Open Access
Tianshu Liu, Yan Wang, Mengfan Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2927 - 2927

Published: March 2, 2024

Ischemic stroke is associated with a high mortality rate, and effective treatment strategies are currently lacking. In this study, we aimed to develop novel nano delivery system treat ischemic via intranasal administration. A three-factor Box–Behnken experimental design was used optimize the formulation of liposomes co-loaded Panax notoginseng saponins (PNSs) Ginsenoside Rg3 (Rg3) (Lip-Rg3/PNS). Macrophage membranes were coated onto surface optimized target site brain. The double-loaded disguised by macrophage (MM-Lip-Rg3/PNS) spherical, in “shell–core” structure, encapsulation rates 81.41% (PNS) 93.81% (Rg3), showed good stability. vitro, MM-Lip-Rg3/PNS taken up brain endothelial cells clathrin-dependent endocytosis micropinocytosis pathways. Network pharmacology experiments predicted that could regulate multiple signaling pathways reducing apoptosis inflammatory responses. After 14 days MM-Lip-Rg3/PNS, survival weight, neurological score middle cerebral artery occlusion (MCAO) rats significantly improved. hematoxylin eosin (H&E) TUNEL staining results can reduce neuronal cell infiltration protect vivo biological have shown free Rg3, PNS, alleviate inflammation apoptosis, especially indicating biomimetic improve therapeutic effects drugs. Overall, potential targeted for therapy.

Language: Английский

Citations

7
Reactivity of Acrylamides Causes Cytotoxicity and Activates Oxidative Stress Response DOI Creative Commons
Julia Huchthausen, Beate I. Escher, Nico Grasse

et al.

Chemical Research in Toxicology, Journal Year: 2023, Volume and Issue: 36(8), P. 1374 - 1385

Published: Aug. 2, 2023

Acrylamides are widely used industrial chemicals that cause adverse effects in humans or animals, such as carcinogenicity neurotoxicity. The excess toxicity of these reactive electrophilic is especially interesting, it mostly triggered by covalent reactions with biological nucleophiles, DNA bases, proteins, peptides. cytotoxicity and activation oxidative stress response 10 (meth)acrylamides measured three reporter gene cell lines occurred at similar concentrations. Most acrylamides exhibited high toxicity, while methacrylamides acted baseline toxicants. showed no reactivity toward the hard nucleophile 2-deoxyguanosine (2DG) within 24 h, only reacted soft glutathione (GSH). Second-order degradation rate constants (kGSH) were for all N,N'-methylenebis(acrylamide) (NMBA) showing highest kGSH (134.800 M-1 h-1) N,N-diethylacrylamide (NDA) lowest (2.574 h-1). Liquid chromatography coupled to high-resolution mass spectrometry was confirm GSH conjugates a double conjugate formed NMBA. differences between could be explained charge density carbon atoms because electron-donating inductive effect methyl group lowered their electrophilicity thus reactivity. energy unoccupied molecular orbital steric hindrance. Cytotoxicity linearly correlated second-order reaction GSH. hence not detoxification mechanism but also leads disturbances redox balance, making cells more vulnerable oxygen species. cells, lack led toxicity.

Language: Английский

Citations

12
Mechanistic progression of acrylamide neurotoxicity linked to neurodegeneration and mitigation strategies DOI Creative Commons
Nisreen Rajeh

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(4)

Published: April 2, 2024

Abstract Acrylamide (AA) is a potential neurotoxic chemical used widely in numerous large-scale industries and molecular research labs. It common toxic contaminant potato grain-based food products prepared at high temperatures. AA has received serious attention due to the cumulative level exposure human population regularly through dietary, environmental, cosmetics routes other than just occupational exposure. well-characterized neurotoxin many rodents studies; however mechanistic pathway lacks detailed characterization. Putatively, exerts its effects primarily mediated by terminal nerve damage inhibition of neurotransmission upon formation irreversible AA-neuronal protein adducts. Other contributors AA-induced neuropathological alterations involve an imbalance redox neuronal cells, kinesin-based axonal transport, increased apoptosis, degenerative changes cholinergic dopaminergic neurons, hyperphosphorylation Tau. These neurological substantiate prognosis pathological development severe neurodegenerative diseases. This review summarizes possible advances understanding mechanisms neurotoxicity clinical implications. Furthermore, we also discuss therapeutic mitigation strategies counter health implications AA.

Language: Английский

Citations

4
Assessing how well chemically and ultrasonically assisted techniques degrade hydrolyzed polyacrylamide solution DOI
Nasir Khan, Xiaoyu Gu, Lei Zhang

et al.

Petroleum Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: Jan. 2, 2025

Current study uses chemical agents and ultrasonic waves to degrade the partially hydrolyzed polyacrylamide (PHPAM) solution. Due decrease in effectiveness of solution, it is essential degel leftover Various chemicals, including hydrogen peroxide (H2O2) chlorine dioxide (ClO2), remove residue. However, due consumable procurement environmental insecurity, this technology not appealing. In contrast, perform well degradation are more reliable, cost-effective, environmentally friendly. The transducer #1 with a frequency 18.0 KHz functioned admirably, successfully degrading polymer After 10 min irradiation, solution's viscosity dropped from 114 6.45 mPa-s. Transducers #2 #3, frequencies 20.0 25.0 KHz, reduced 6.62 6.5 mPa-s, respectively. ClO2 degrades effectively than H2O2 its higher oxidizing potential, reducing 15.8 mPa-s after 12 h. Ultrasonic-assisted PHPAM mainly cavitation, heat energy, hydroxyl radical (HO∙) generation, while treatment involves radicals nascent oxygen [O]. This first compare chemical-assisted ultrasonic-aided polyacrylamide.

Language: Английский

Citations

0