Landscape of human organoids: Ideal model in clinics and research

The Innovation, Journal Year: 2024, Volume and Issue: 5(3), P. 100620 - 100620

Published: April 1, 2024

In the last decade, organoid research has entered a golden era, signifying pivotal shift in biomedical landscape. The year 2023 marked milestone with publication of thousands papers this arena, reflecting exponential growth. However, amid burgeoning expansion, comprehensive and accurate overview field been conspicuously absent. Our review is intended to bridge gap, providing panoramic view rapidly evolving We meticulously analyze from eight distinctive vantage points, harnessing our rich experience academic research, industrial application, clinical practice. present deep exploration advances technology, underpinned by long-standing involvement arena. narrative traverses historical genesis organoids their transformative impact across various sectors, including oncology, toxicology, drug development. delve into synergy between avant-garde technologies such as synthetic biology single-cell omics discuss role tailoring personalized medicine, enhancing high-throughput screening, constructing physiologically pertinent disease models. analysis reflective discourse provide dive existing landscape emerging trends technology. spotlight technological innovations, methodological evolution, broadening spectrum applications, emphasizing revolutionary influence discovery, other fields. Looking ahead, we cautiously anticipate future developments especially its potential implications for patient care, new avenues research. trust that will be an asset researchers, clinicians, patients keen interest medical strategies. offer broad prospective capabilities encompassing wide range current applications. summary, attempt field. reflections, summaries, projections might useful researchers hope contribute shaping trajectory dynamic advancing

Language: Английский

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Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 208, P. 115237 - 115237

Published: March 5, 2024

Language: Английский

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The Promise and Potential of Brain Organoids

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(21)

Published: Jan. 22, 2024

Brain organoids are 3D in vitro culture systems derived from human pluripotent stem cells that self-organize to model features of the (developing) brain. This review examines techniques behind organoid generation, their current and potential applications, future directions for field. possess complex architecture containing various neural cell types, synapses, myelination. They have been utilized toxicology testing, disease modeling, infection studies, personalized medicine, gene-environment interaction studies. An emerging concept termed Organoid Intelligence (OI) combines with artificial intelligence generate learning memory, goals modeling cognition enabling biological computing applications. allow neuroscience studies not previously achievable traditional techniques, transform drug development, understanding brain development disorders. The aspirational vision OI parallels origins intelligence, efforts underway map a roadmap toward its realization. In summary, constitute disruptive technology is rapidly advancing gaining traction across multiple disciplines.

Language: Английский

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Orthotopic and metastatic tumour models in preclinical cancer research

Pharmacology & Therapeutics, Journal Year: 2024, Volume and Issue: 257, P. 108631 - 108631

Published: March 11, 2024

Mouse models of disease play a pivotal role at all stages cancer drug development. Cell-line derived subcutaneous tumour are predominant in early discovery, but there is growing recognition the importance more complex orthotopic and metastatic for understanding both target biology correct tissue context, impact microenvironment immune system responses to treatment. The aim this review highlight value that bring study development while pointing out those most likely be encountered literature. Important developments models, such as increasing use passage patient material (PDXs, organoids) humanised mouse discussed, these approaches have potential increase predictive preclinical studies, ultimately improve success rate anticancer drugs clinical trials.

Language: Английский

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Patient-derived organoids in human cancer: a platform for fundamental research and precision medicine

Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)

Published: Feb. 12, 2024

Abstract Cancer is associated with a high degree of heterogeneity, encompassing both inter- and intra-tumor along considerable variability in clinical response to common treatments across patients. Conventional models for tumor research, such as vitro cell cultures vivo animal models, demonstrate significant limitations that fall short satisfying the research requisites. Patient-derived organoids, which recapitulate structures, specific functions, molecular characteristics, genomics alterations expression profiles primary tumors. They have been efficaciously implemented illness portrayal, mechanism exploration, high-throughput drug screening assessment, discovery innovative therapeutic targets potential compounds, customized treatment regimen cancer In contrast conventional organoids offer an intuitive, dependable, efficient model by conserving phenotypic, genetic diversity, mutational attributes originating tumor. Nevertheless, organoid technology also confronts bottlenecks challenges, how comprehensively reflect microenvironment, angiogenesis, reduce costs, establish standardized construction processes while retaining reliability. This review extensively examines use techniques fundamental precision medicine. It emphasizes importance patient-derived biobanks development, screening, safety evaluation, personalized Additionally, it evaluates application experimental better understand mechanisms The intent this explicate significance present new avenues future research.

Language: Английский

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Decentralized Biobanking App for Research Transparency and Patient Engagement: Participatory Design (Preprint)

JMIR Human Factors, Journal Year: 2025, Volume and Issue: 12, P. e59485 - e59485

Published: Jan. 14, 2025

Patient-derived biospecimens are invaluable tools in biomedical research. Currently, there no mechanisms for patients to follow along and learn about the uses of their donated samples. Incorporating as stakeholders meaningfully engaging them research first requires transparency activities. In this paper, we describe use participatory design methods build a decentralized biobanking "de-bi" mobile app where could biobanking, track specimens, engage with ongoing via patient-friendly interfaces overlaying institutional biobank databases, initially developed breast cancer case. This occurred 2 phases. phase 1, designed screens from which involving We embedded these survey (n=94) gauge patients' interests regarding types feedback engagement opportunities; responses were probed during 6 comprehensive follow-up interviews. then held an immersive workshop participants (approximately 50) provided general our approach, codesign subset (n=15) targeted on screen designs. For 2, refined user functional prototype consultation ensure regulatory compliance, workflow compatibility, composability local data architectures. presented at second workshop, (n=25, across 9 groups) shared thoughts app's usability design. phase, conducted cognitive walkthroughs (n=13) gain in-depth in-app task navigation. Most (61/81, 75%) interested learning outcomes 49% (41/83) connecting others same diagnosis. Participants (47/60, 78%) expressed strong interest receiving summaries scientific information scientists using biospecimens. The identified confusion terminology presentation (eg, 9/15, 60% co-designers unclear "in use"), though many appreciated ability view personal (7/15, 47%), most excited (12/15, 80%). all groups found valuable. Moreover, 44% (5/9) noted they did not like onboarding process, was echoed walkthroughs. Walkthroughs further confirmed biospecimen tracking, 23% (3/13) had finding any app. These findings guided refinements onboarding, design, experience. Designing patient-facing that displays biobanked specimens can facilitate greater Co-designing patient related research, improved data, ensured preparation pilot study.

Language: Английский

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Role of Patient-Derived Models of Cancer in Translational Oncology

Cancers, Journal Year: 2022, Volume and Issue: 15(1), P. 139 - 139

Published: Dec. 26, 2022

Cancer is a heterogeneous disease. Each individual tumor unique and characterized by structural, cellular, genetic molecular features. Therefore, patient-derived cancer models are indispensable tools in research have been actively introduced into the healthcare system. For instance, provide good reproducibility of susceptibility resistance cells against drugs, allowing personalized therapy for patients. In this article, we review advantages disadvantages following cancer: (1) PDC—patient-derived cell culture, (2) PDS—patient-derived spheroids PDO—patient-derived organoids, (3) PDTSC—patient-derived tissue slice cultures, (4) PDX—patient-derived xenografts, humanized PDX, as well PDXC—PDX-derived cultures PDXO—PDX-derived organoids. We also an overview current clinical investigations new developments area models. Moreover, attention paid to databases models, which collected specialized repositories. believe that widespread use will improve our knowledge biology contribute development more effective treatment strategies.

Language: Английский

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Application of CRISPR/Cas9 Technology in Cancer Treatment: A Future Direction

Current Oncology, Journal Year: 2023, Volume and Issue: 30(2), P. 1954 - 1976

Published: Feb. 6, 2023

Gene editing, especially with clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9), has advanced gene function science. editing’s rapid advancement increased its medical/clinical value. Due to great specificity and efficiency, CRISPR/Cas9 can accurately swiftly screen the whole genome. This simplifies disease-specific therapy. To study tumor origins, development, metastasis, change genomes. In recent years, treatment research increasingly employed this method. treat cancer by removing genes or correcting mutations. Numerous preliminary studies have been conducted in relevant fields. may gene-level tumors. CRISPR/Cas9-based personalized targeted medicines shape treatment. review examines for therapy research, which will be helpful providing references future on pathogenesis of malignancy

Language: Английский

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Engineering In vitro Models: Bioprinting of Organoids with Artificial Intelligence

Cyborg and Bionic Systems, Journal Year: 2023, Volume and Issue: 4

Published: Jan. 1, 2023

In the last decade, organoids have gained popularity for developing mini-organs to support advancements in study of organogenesis, disease modeling, and drug screening and, subsequently, development new therapies. To date, such cultures been used replicate composition functionality organs as kidney, liver, brain, pancreas. However, depending on experimenter, culture environment cell conditions may slightly vary, resulting different organoids; this factor significantly affects their application development, especially during quantification. Standardization context can be achieved using bioprinting technology-an advanced technology that print various cells biomaterials at desired locations. This offers numerous advantages, including manufacturing complex three-dimensional biological structures. Therefore, addition standardization organoids, organoid engineering facilitate automation fabrication process well a closer mimicry native organs. Further, artificial intelligence (AI) has currently emerged an effective tool monitor control quality final developed objects. Thus, technology, AI combined obtain high-quality vitro models multiple applications.

Language: Английский

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Brain organoids are new tool for drug screening of neurological diseases

Neural Regeneration Research, Journal Year: 2023, Volume and Issue: 0(0), P. 0 - 0

Published: Jan. 1, 2023

At the level of in vitro drug screening, development a phenotypic analysis system with high-content screening at core provides strong platform to support high-throughput screening. There are few systematic reports on brain organoids, as new three-dimensional model, terms model stability, key fingerprint, and schemes, particularly regarding strategies for massive numbers traditional Chinese medicine monomers. This paper reviews organoids advantages over induced neurons or cells simulated diseases. The also highlights prospects from induction criteria schemes based characteristics application system.

Language: Английский

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