Materials Today Bio,
Journal Year:
2024,
Volume and Issue:
26, P. 101079 - 101079
Published: May 5, 2024
As
a
booming
engineering
technology,
the
microfluidic
chip
has
been
widely
applied
for
replicating
complexity
of
human
intestinal
micro-physiological
ecosystems
in
vitro.
Biosensors,
3D
imaging,
and
multi-omics
have
to
engineer
more
sophisticated
barrier-on-chip
platforms,
allowing
improved
monitoring
physiological
processes
enhancing
performance.
In
this
review,
we
report
cutting-edge
advances
techniques
establishment
evaluation
barrier
platforms.
We
discuss
different
design
principles
microfabrication
strategies
gut
models
Further,
comprehensively
cover
complex
cell
types
(e.g.,
epithelium,
organoids,
endothelium,
microbes,
immune
cells)
controllable
extracellular
microenvironment
parameters
oxygen
gradient,
peristalsis,
bioflow,
gut-organ
axis)
used
recapitulate
main
structural
functional
barriers.
also
present
current
multidisciplinary
technologies
indicators
evaluating
morphological
structure
integrity
established
Finally,
highlight
challenges
future
perspectives
accelerating
broader
applications
these
platforms
disease
simulation,
drug
development,
personalized
medicine.
Hence,
review
provides
comprehensive
guide
development
microfluidic-based
MedComm,
Journal Year:
2024,
Volume and Issue:
5(9)
Published: Aug. 28, 2024
Ovarian
cancer
is
high
recurrence
and
mortality
malignant
tumor.
The
most
common
ovarian
was
High-Grade
Serous
Cancer.
However,
Cancer
organoid
rare,
which
with
patient
immune
microenvironment
blood
vessels
even
absence.
Here,
we
report
a
novel
system
derived
from
samples.
These
organoids
recapitulate
organoids'
histological
molecular
heterogeneity
while
preserving
the
critical
vessels,
as
evidenced
by
presence
of
Small Methods,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: Oct. 26, 2023
Abstract
Basic
and
clinical
cancer
research
requires
tumor
models
that
consistently
recapitulate
the
characteristics
of
prima
tumors.
As
ex
vivo
3D
cultures
patient
cells,
patient‐derived
organoids
possess
biological
properties
primary
tumors
are
therefore
excellent
preclinical
for
research.
Patient‐derived
can
be
established
using
tissues,
peripheral
blood,
pleural
fluid,
ascites,
other
samples
containing
cells.
Circulating
cells
acquired
by
non‐invasive
sampling
feature
dynamic
circulation
high
heterogeneity.
cell‐derived
prospective
tools
monitoring
mutation
evolution
profiles
because
they
reflect
heterogeneity
original
to
a
certain
extent.
This
review
discusses
advantages
applications
organoids.
Meanwhile,
this
work
highlights
functions
circulating
latest
advancement
in
organoids,
potential
application
challenges
technology.
Cancers,
Journal Year:
2023,
Volume and Issue:
15(9), P. 2570 - 2570
Published: April 30, 2023
Colorectal
cancer
(CRC)
is
the
third
most
prevalent
malignancy
worldwide
and
in
both
sexes.
Numerous
animal
models
for
CRC
have
been
established
to
study
its
biology,
namely
carcinogen-induced
(CIMs)
genetically
engineered
mouse
(GEMMs).
CIMs
are
valuable
assessing
colitis-related
carcinogenesis
studying
chemoprevention.
On
other
hand,
GEMMs
proven
be
useful
evaluating
tumor
microenvironment
systemic
immune
responses,
which
contributed
discovery
of
novel
therapeutic
approaches.
Although
metastatic
disease
can
induced
by
orthotopic
injection
cell
lines,
resulting
not
representative
full
genetic
diversity
due
limited
number
lines
suitable
this
purpose.
patient-derived
xenografts
(PDX)
reliable
preclinical
drug
development
their
ability
retain
pathological
molecular
characteristics.
In
review,
authors
discuss
various
murine
with
a
focus
on
clinical
relevance,
benefits,
drawbacks.
From
all
discussed,
will
continue
an
important
tool
advancing
our
understanding
treatment
disease,
but
additional
research
required
find
model
that
correctly
reflect
pathophysiology
CRC.
Aggregate,
Journal Year:
2024,
Volume and Issue:
5(2)
Published: Jan. 4, 2024
Abstract
Organoids
have
emerged
as
a
powerful
platform
for
studying
complex
biological
processes
and
diseases
in
vitro.
However,
most
studies
focused
on
individual
organoids,
overlooking
the
inter‐organ
interactions
vivo
limiting
physiological
relevance
of
models.
To
address
this
limitation,
development
multi‐organoid
system
has
gained
considerable
attention.
This
aims
to
recapitulate
communication
enable
study
processes.
review
provides
comprehensive
overview
recent
advancements
organoid
engineering
emerging
strategies
constructing
system.
First,
we
highlight
critical
mechanical,
structural,
biochemical
factors
involved
designing
suitable
materials
growth
different
organoids.
Additionally,
discuss
incorporation
dynamic
culture
environments
enhance
inter‐organoid
communication.
Furthermore,
explore
techniques
manipulating
morphogenesis
spatial
positioning
organoids
establish
effective
networks.
We
summarize
achievements
utilizing
vitro,
including
assembloids
microfluidic
platforms.
Lastly,
existing
challenges
opportunities
developing
from
its
technical
bottlenecks
scalability
applications
toward
human
diseases.
Materials Today Bio,
Journal Year:
2024,
Volume and Issue:
26, P. 101079 - 101079
Published: May 5, 2024
As
a
booming
engineering
technology,
the
microfluidic
chip
has
been
widely
applied
for
replicating
complexity
of
human
intestinal
micro-physiological
ecosystems
in
vitro.
Biosensors,
3D
imaging,
and
multi-omics
have
to
engineer
more
sophisticated
barrier-on-chip
platforms,
allowing
improved
monitoring
physiological
processes
enhancing
performance.
In
this
review,
we
report
cutting-edge
advances
techniques
establishment
evaluation
barrier
platforms.
We
discuss
different
design
principles
microfabrication
strategies
gut
models
Further,
comprehensively
cover
complex
cell
types
(e.g.,
epithelium,
organoids,
endothelium,
microbes,
immune
cells)
controllable
extracellular
microenvironment
parameters
oxygen
gradient,
peristalsis,
bioflow,
gut-organ
axis)
used
recapitulate
main
structural
functional
barriers.
also
present
current
multidisciplinary
technologies
indicators
evaluating
morphological
structure
integrity
established
Finally,
highlight
challenges
future
perspectives
accelerating
broader
applications
these
platforms
disease
simulation,
drug
development,
personalized
medicine.
Hence,
review
provides
comprehensive
guide
development
microfluidic-based
