High expression of ARPC1B correlates with immune infiltration and poor outcomes in glioblastoma DOI Creative Commons
Chuangxin Liao, Wenli Chen,

Guixin Xu

et al.

Biochemistry and Biophysics Reports, Journal Year: 2023, Volume and Issue: 37, P. 101619 - 101619

Published: Dec. 19, 2023

To investigate the role of ARPC1B in GBM and its prognostic value.

Language: Английский

A pan-cancer analysis reveals the oncogenic and immunological role of insulin-like growth factor 2 mRNA-binding protein family members DOI Creative Commons
Fangfang Zeng, Liuyan Chen, Jing Li

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 15, 2025

Abstract Purpose To investigate the expression and clinical significance of insulin-like growth factor 2 mRNA-binding protein family members (IGF2BPs) in pan-cancer evaluate their potential as targets for tumor immunotherapy. Methods Based on data from cancer genome atlas (TCGA) database, analysis was conducted to examine IGF2BPs twenty-two tumors. Results Differential showed high most tissues. Survival mutation analyses suggested that overexpression associated with poor prognosis status certain Methylation revealed methylation levels IGF2BP1/2/3 tumors were intricately linked mRNA expression, patient prognosis, immune cell infiltration. Enrichment indicated abnormal various common tumor-related pathways different tumors, including AMPK, Hippo, PI3K-Akt, EMT, p53. In addition, correlation closely related immunotherapy-related indicators (immune infiltration, major histocompatibility complex (MHC), checkpoints, burden (TMB), microsatellite instability (MSI)) some Drug sensitivity sensitive chemotherapeutic drugs (alvocidib, dasatinib, trametinib, selumetinib). Conclusion exhibit significantly are pathological stage, mutational status, levels, relevant immunotherapy multiple Moreover, may play an oncogenic role by activating signaling pathways. Therefore, be prognostic markers therapy drug therapy.

Language: Английский

Citations

1
Low-density lipoprotein receptor promotes crosstalk between cell stemness and tumor immune microenvironment in breast cancer: a large data-based multi-omics study DOI Creative Commons
Qihang Yuan, Xiaona Lu, Hui Guo

et al.

Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 30, 2023

Abstract Background Tumor cells with stemness in breast cancer might facilitate the immune microenvironment’s suppression process and led to anti-tumor effects. The primary objective of this study was identify potential targets disrupt communication between cell microenvironment. Methods In study, we initially isolated tumor varying degrees using a spheroid formation assay. Subsequently, employed RNA-seq proteomic analyses genes associated through gene trend analysis. These stemness-related were then subjected pan-cancer analysis elucidate their functional roles broader spectrum types. data 3132 patients clinical obtained from public databases. Using identified genes, constructed two distinct subtypes, denoted as C1 C2. We subsequently conducted comprehensive differences these subtypes pathway enrichment methodology infiltration algorithms. Furthermore, key immune-related by employing lasso regression Cox survival model. vitro experiments ascertain regulatory impact on stemness. Additionally, utilized delineate functions attributed gene. Lastly, single-cell RNA sequencing (scRNA-seq) conduct more examination gene’s role within Results our set 65 displaying capabilities. analysis, pinpointed 41 that held prognostic significance. observed C2 subtype exhibited higher capacity compared displayed aggressive malignancy profile. Further Lasso-Cox algorithm LDLR pivotal It became evident played crucial shaping demonstrated regulated cancer. Immune determined inhibited proliferation promote progression. scRNA-seq discovered associations marker tissues. Moreover, expression levels cells, further emphasizing its relevance context Conclusion is an important regulates enhances crosstalk

Language: Английский

Citations

18
Emerging Roles and Mechanisms of RNA Modifications in Neurodegenerative Diseases and Glioma DOI Creative Commons
Ami Kobayashi, Yosuke Kitagawa, Ali M. Nasser

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 457 - 457

Published: March 5, 2024

Despite a long history of research, neurodegenerative diseases and malignant brain tumor gliomas are both considered incurable, facing challenges in the development treatments. Recent evidence suggests that RNA modifications, previously as static components intracellular RNAs, fact dynamically regulated across various species cells play critical role major biological processes nervous system. Innovations next-generation sequencing have enabled accurate detection modifications on bases sugars within molecules. These influence stability transportation RNA, crucially affect its translation. This review delves into existing knowledge to offer comprehensive inventory these different species. The detailed regulatory functions roles system discussed with focus gliomas. article presents overview fundamental mechanisms emerging diseases, which can facilitate creation innovative diagnostics therapeutics for conditions.

Language: Английский

Citations

4
Dual Disruption of EGFR/PI3K Signaling: IGF2BP2 Targeting Reverses Anti-EGFR Resistance in CAFs-Infiltrated Oral Squamous Cell Carcinoma DOI Open Access

Yaying Hu,

Tianshuang Zhu,

Sheng Nong

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3941 - 3941

Published: April 22, 2025

RNA-binding proteins (RBPs) critically regulate post-transcriptional gene networks, yet their roles and mechanisms in oral squamous cell carcinoma (OSCC) remain underexplored. Dysregulated RBPs were identified through integrated analysis of RNA-seq single-cell RNA-seq. The oncogenic functions IGF2BP2 evaluated tissue microarrays, CCK-8, transwell assays, mouse xenografts, Igf2bp2-deficient models tongue SCC (TSCC). Subsequently, we utilized RNA-seq, RIP-seq, RIP/MeRIP-qPCR, dual-luciferase reporter assays to investigate IGF2BP2-target genes. Furthermore, co-culture system TSCC used validate the therapeutic effect inhibitor. was most markedly upregulated RBP OSCC cells cancer-associated fibroblasts (CAFs), correlating with unfavorable prognosis. deprivation significantly impaired human proliferation metastasis, delayed onset. Mechanistically, stabilized EGFR PIK3R1 mRNA via m6A-dependent interactions, thereby sustaining activation EGFR/PI3K/AKT axis. Pharmacological inhibition exhibited anti-OSCC efficacy vivo vitro by concurrently suppressing PI3K/AKT pathway activity, overcoming anti-EGFR resistance resulting from cell-intrinsic hyperactivation CAF-secreted factors. Our findings as a master regulator progression promising target, offering an alternative strategy for patients suffering resistance.

Language: Английский

Citations

0
Reduced expression of insulin like growth factor-2 mRNA binding protein 2 (IMP2) elevates TAZ via IMP3 in triple-negative breast cancer DOI

Shubhashree Parimita,

Amitava Das, Sanjoy Samanta

et al.

Gene Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102209 - 102209

Published: March 1, 2025

Language: Английский

Citations

0
Systematic analysis of IGF2BP family members in non-small-cell lung cancer DOI Creative Commons

Liping Gong,

Qin Liu, Ming Jia

et al.

Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)

Published: June 12, 2024

The insulin-like growth factor-2 mRNA-binding proteins 1, 2, and 3 (IGF2BP1, IGF2BP2, IGF2BP3) are known to be involved in tumorigenesis, metastasis, prognosis, cancer immunity various human cancers, including non-small cell lung (NSCLC). However, the literature on NSCLC largely omits specific context of squamous carcinoma (LUSC), an oversight we aim address.

Language: Английский

Citations

2
IGF2BP3 Enhances the Growth of Hepatocellular Carcinoma Tumors by Regulating the Properties of Macrophages and CD8+ T Cells in the Tumor Microenvironment DOI Open Access

Lingyu Ma,

Jiayu Jiang, Si Qin

et al.

Journal of Clinical and Translational Hepatology, Journal Year: 2023, Volume and Issue: 000(000), P. 000 - 000

Published: Aug. 1, 2023

Background and AimsOverexpression of IGF2BP3 is associated with the prognosis hepatocellular carcinoma (HCC). However, its role in regulating tumor immune microenvironment (TME) not well characterized. Here, we investigated effects on macrophages CD8+ T cells within TME HCC.

Language: Английский

Citations

4
The RNA-binding protein IGF2BP1 regulates stability of mRNA transcribed from FOXM1 target genes in hypermitotic meningiomas DOI Creative Commons
Nathan K. Leclair, Calixto‐Hope G. Lucas, Kanish Mirchia

et al.

Acta Neuropathologica, Journal Year: 2024, Volume and Issue: 148(1)

Published: Aug. 23, 2024

Language: Английский

Citations

1
The Emerging Role of IGF2BP2 in Cancer Therapy Resistance: From Molecular Mechanism to Future Potential DOI Open Access

D Li,

Shi‐Qi Hu, Jiarong Ye

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12150 - 12150

Published: Nov. 12, 2024

Tumor resistance is one of the primary reasons for cancer treatment failure, significantly limiting options and efficacy therapies. Therefore, overcoming has become a critical factor in improving outcomes. IGF2BP2, as reader m6A methylation, plays pivotal role post-transcriptional regulation RNA through methylation sites. It not only contributes to initiation progression but also key tumor drug resistance. This review provides comprehensive summary mechanisms by which IGF2BP2 therapy resistance, with aim chemotherapy treatment. Advancing research this area crucial developing more effective therapies that could improve quality life patients.

Language: Английский

Citations

1
Three Liquid-Liquid Phase Separation-Related Genes Associated with Prognosis in Glioma DOI Creative Commons
Ling Lv, Xin Zhang, Yajun Liu

et al.

Pharmacogenomics and Personalized Medicine, Journal Year: 2024, Volume and Issue: Volume 17, P. 171 - 181

Published: April 1, 2024

Purpose: Dysregulated liquid-liquid phase separation (LLPS) instigates tumorigenesis through biomolecular condensate dysfunction.However, the association between LLPS-associated genes and glioma remains underexplored.Patients Methods: Differentially expressed (DEGs) of were obtained from GSE50161 dataset, including 34 13 normal samples.We analyzed differentially LLPS-related in public databases.These informed refined molecular subtyping on TCGA-glioma dataset.CIBERSORT assessed immune cell infiltration across three subclusters.A prognostic model was devised using univariate lasso Cox regressions intersecting genes.Prognostic gene expression validated cells via RT-qPCR.Results: A total 673 identified glioma.Three distinct subtypes (C1, C2, C3) with a marked variance checkpoint PD1 PDL1.Differences observed subtypes.In addition, tri-gene signature (TAGLN2, NTNG2, IGF2BP2) derived significant survival differences high low-risk groups.The displayed impressive AUC values for 1, 3, 5-year both training validation sets.Further analysis highlighted notable correlation samples.Furthermore, we found upregulation TAGLN2 IGF2BP2 downregulation NTNG2 tumors cells. Conclusion:This study innovatively uncovers role tumor grading prognosis.The constructed holds promise enhancing personalized prognosis assessments optimizing immunotherapy strategies patients.

Language: Английский

Citations

1