Tumor Prognostic Risk Model Related to Monocytes/Macrophages in Hepatocellular Carcinoma Based on Machine Learning and Multi-Omics

Biological Procedures Online, Journal Year: 2025, Volume and Issue: 27(1)

Published: March 10, 2025

Tumor-associated macrophages (TAMs) are crucial in hepatocellular carcinoma (HCC) development and invasion. This study explores monocyte/ macrophage-associated gene expression profiles HCC, constructs a prognostic model based on these genes, examines its relationship with drug resistance immune therapy responses. Single-cell RNA sequencing(scRNA-seq) data from 10 HCC tissue biopsy samples, totaling 24,597 cells, were obtained the GEO database to identify monocyte/macrophage-associated genes. A was constructed validated using external datasets Western blot. Relationships between model, clinical correlates, sensitivity, responses investigated. From scRNA-seq data, 2,799 monocyte/macrophage marker genes identified. Using TCGA dataset, single-gene UQCRH constructed, stratifying patients into high-risk low-risk groups overall survival rates. High-risk group showed reduced rates higher tumor tissues. blot analysis further confirmed elevated of cell lines. Spatial transcriptomics revealed that high co-localized malignant cells tissue. Drug sensitivity had lower sorafenib axitinib. Immune response indicated poorer outcomes group, more pronounced APC inhibition weaker IFN-II response. Clinical indicator positive correlation Enrichment associated molecules involvement oxidative phosphorylation mitochondrial electron transport. introduces for The predicts patient treatment outcomes, identifying individuals varying sensitivities suppression states.

Language: Английский

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Neutrophil infiltration in peritoneal metastasis affects prognosis in patients with ovarian cancer

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

The impact on prognosis of neutrophil infiltration in the microenvironment ovarian cancer remains to be elucidated. In this study, we analyzed association between peritoneal metastasis and prognosis. Furthermore, correlation number peripheral blood neutrophils, degree neutropenia, vascular endothelial area, stromal cells. Thirty-four specimens each primary metastatic advanced with high-grade serous carcinoma histology were taken. Staining for MPO, CD31, αSMA was performed all specimens. We investigated among tissue infiltration, prognosis, indicators from tests. Survival analysis showed that tumor had no whereas high disseminated significantly shortened progression-free survival overall survival. No found area cells, Neutrophil into lesions omentum has a significant results present study may provide insights refining clinical approaches.

Language: Английский

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Natural medicines target tumor vascular microenvironment to inhibit tumor

Genes & Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 101623 - 101623

Published: April 1, 2025

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Chimeric antigen receptor-modified T cells therapy in prostate cancer: A comprehensive review on the current state and prospects

Heliyon, Journal Year: 2023, Volume and Issue: 9(8), P. e19147 - e19147

Published: Aug. 1, 2023

Recent immunotherapy research has focused on chimeric antigen receptor-modified T cells (CAR-Ts). CAR-T therapies have been clinically applied to manage hematologic malignancies with satisfactory effectiveness. However, the application of in solid tumors remains challenging. Even so, current immunotherapies for prostate cancer (PCa) shown some promise, giving hope patients advanced metastatic PCa. This review aimed elucidate different types tumor-associated targets, such as prostate-specific membrane and stem cell antigen, their effects. The status corresponding targets clinical through applications was also discussed. To improve efficacy immunotherapy, we addressed possible multimodal chemotherapy, combined therapies. obstacles were concisely elaborated. Further studies should aim discover novel potential establish new models by overcoming inherent barriers tumors, tumor heterogeneity immunosuppressive nature microenvironment.

Language: Английский

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The Pluripotent Path to Immunotherapy

Experimental Hematology, Journal Year: 2024, Volume and Issue: unknown, P. 104648 - 104648

Published: Sept. 1, 2024

Language: Английский

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Protumoral lipid droplet–loaded macrophages are enriched in human glioblastoma and can be therapeutically targeted

Science Translational Medicine, Journal Year: 2024, Volume and Issue: 16(771)

Published: Oct. 30, 2024

Glioblastoma presents a formidable clinical challenge because of its complex microenvironment. Here, we characterized tumor-associated foam cells (TAFs), type lipid droplet–loaded macrophage, in human glioblastoma. Through extensive analyses patient tumors, together with vitro and vivo investigations, found that TAFs exhibit distinct protumorigenic characteristics related to hypoxia, mesenchymal transition, angiogenesis, impaired phagocytosis, their presence correlates worse outcomes for patients glioma. We further demonstrated TAF formation is facilitated by scavenging from extracellular vesicles released glioblastoma cells. targeting key enzymes involved droplet formation, such as diacylglycerol O -acyltransferase or long-chain acyl-CoA synthetase, effectively disrupted functionality. Together, these data highlight prominent immune cell population provide insights into contribution the tumor Disrupting target may represent an avenue future therapeutic development

Language: Английский

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Cell-autonomous IL6ST activation suppresses prostate cancer development via STAT3/ARF/p53-driven senescence and confers an immune-active tumor microenvironment

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Oct. 31, 2024

Prostate cancer ranks as the second most frequently diagnosed in men worldwide. Recent research highlights crucial roles IL6ST-mediated signaling pathways play development and progression of various cancers, particularly through hyperactivated STAT3 signaling. However, molecular programs mediated by IL6ST/STAT3 prostate are poorly understood.

Language: Английский

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A Comparative Study of Different Protocols for Isolation of Murine Neutrophils from Bone Marrow and Spleen

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(24), P. 17273 - 17273

Published: Dec. 8, 2023

Neutrophils are considered as the main player in innate immunity. In last few years, it has been shown that they involved different physiological conditions and diseases. However, progress field of neutrophil biology is relatively slow due to existing difficulties isolation maintenance culture. Here we compare four protocols based on density-gradient immunomagnetic methods for murine neutrophils from bone marrow spleen. Neutrophil was performed using Ficoll 1.077/1.119 g/mL density gradient, 1.083/1.090/1.110 gradient method negative positive selection. The were compared with respect sample purity, cell viability, yield, cost. functionality isolated checked by NETosis analysis oxidative burst test. Obtained data revealed given purity/yield/viability/cost ratio protocol centrifugation recommended marrow, whereas selection Dynabeads splenic neutrophils.

Language: Английский

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Chemotherapy-induced neutropenia elicits metastasis formation in mice by promoting proliferation of disseminated tumor cells

OncoImmunology, Journal Year: 2023, Volume and Issue: 12(1)

Published: Aug. 1, 2023

Chemotherapy is the standard of care for most malignancies. Its tumor debulking effect in adjuvant or neoadjuvant settings unquestionable, although secondary effects have been reported that paradoxically promote metastasis. affects hematopoietic precursors leading to myelosuppression, with neutropenia being main hematological toxicity induced by cytotoxic therapy. We used renal and lung murine models metastatic study chemotherapy-induced (CIN) process. Cyclophosphamide doxorubicin, two myelosuppressive drugs, but not cisplatin, increased burden artificial metastases lung, reducing neutrophils. This was recapitulated treatment anti-Ly6G, selective antibody-mediated neutrophil depletion unleashed formation both spontaneous metastasis settings. The cancer dissemination reversed granulocyte-colony stimulating factor-mediated boosting neutrophils combination chemotherapy. CIN affected early colonization quite likely promoting proliferation cells extravasated into at 24-72 hours. did affect late events process, established nor there any on release from primary, whose growth was, fact, somewhat inhibited. work suggests a role associated common side claims deep dive relationship between

Language: Английский

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Metabolites in the Dance: Deciphering Gut-Microbiota-Mediated Metabolic Reprogramming of the Breast Tumor Microenvironment

Cancers, Journal Year: 2024, Volume and Issue: 16(24), P. 4132 - 4132

Published: Dec. 11, 2024

Breast cancer (BC), a major cause of death among women worldwide, has traditionally been linked to genetic and environmental factors. However, emerging research highlights the gut microbiome’s significant role in shaping BC development, progression, treatment outcomes. This review explores intricate relationship between microbiota breast tumor microenvironment, emphasizing how these microbes influence immune responses, inflammation, metabolic pathways. Certain bacterial species either contribute or hinder progression by producing metabolites that affect hormone metabolism, system pathways, cellular signaling. An imbalance bacteria, known as dysbiosis, associated with heightened risk BC, like short-chain fatty acids (SCFAs) enzymes such β-glucuronidase playing key roles this process. Additionally, can impact effectiveness chemotherapy, certain bacteria degrade drugs gemcitabine irinotecan, leading reduced efficacy. Understanding complex interactions may pave way for innovative approaches, including personalized microbiome-targeted therapies, probiotics fecal transplants, offering new hope more effective prevention, diagnosis, BC.

Language: Английский

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