Liquid Biopsy in the Clinical Management of Cancers

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8594 - 8594

Published: Aug. 6, 2024

Liquid biopsy, a noninvasive diagnosis that examines circulating tumor components in body fluids, is increasingly used cancer management. An overview of relevant literature emphasizes the current state liquid biopsy applications care. Biomarkers particularly DNA (ctDNA), RNAs (ctRNA), cells (CTCs), extracellular vesicles (EVs), and other components, offer promising opportunities for early diagnosis, treatment selection, monitoring, disease assessment. The implementation precision medicine has shown significant potential various types, including lung cancer, colorectal breast prostate cancer. Advances genomic molecular technologies such as next-generation sequencing (NGS) digital polymerase chain reaction (dPCR) have expanded utility enabling detection somatic variants actionable alterations tumors. also demonstrated predicting responses, monitoring minimal residual (MRD), assessing heterogeneity. Nevertheless, standardizing techniques, interpreting results, integrating them into clinical routine remain challenges. Despite these challenges, implications management, offering dynamic approach to understanding biology guiding personalized strategies.

Language: Английский

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Enhancing Clinical Utility: Utilization of International Standards and Guidelines for Metagenomic Sequencing in Infectious Disease Diagnosis

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3333 - 3333

Published: March 15, 2024

Metagenomic sequencing has emerged as a transformative tool in infectious disease diagnosis, offering comprehensive and unbiased approach to pathogen detection. Leveraging international standards guidelines is essential for ensuring the quality reliability of metagenomic clinical practice. This review explores implications application diagnosis. By adhering established standards, such those outlined by regulatory bodies expert consensus, healthcare providers can enhance accuracy utility sequencing. The integration into workflows streamline diagnostic processes, improve identification, optimize patient care. Strategies implementing these diagnosis using are discussed, highlighting importance standardized approaches advancing precision initiatives.

Language: Английский

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Exploring the Role of Circulating Cell-Free RNA in the Development of Colorectal Cancer

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 11026 - 11026

Published: July 3, 2023

Circulating tumor RNA (ctRNA) has recently emerged as a novel and attractive liquid biomarker. CtRNA is capable of providing important information about the expression variety target genes noninvasively, without need for biopsies, through use circulating sequencing. The overexpression cancer-specific transcripts increases tumor-derived signal, which overcomes limitations due to low quantities DNA (ctDNA). purpose this work present an up-to-date review current knowledge regarding ctRNAs their status biomarkers address diagnosis, prognosis, prediction, drug resistance colorectal cancer. final section article discusses practical aspects involved in analyzing plasma ctRNA, including storage isolation, detection technologies, clinical applications.

Language: Английский

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A Comprehensive Review on Circulating cfRNA in Plasma: Implications for Disease Diagnosis and Beyond

Diagnostics, Journal Year: 2024, Volume and Issue: 14(10), P. 1045 - 1045

Published: May 17, 2024

Circulating cfRNA in plasma has emerged as a fascinating area of research with potential applications disease diagnosis, monitoring, and personalized medicine. RNA sequencing technology allows for the non-invasive collection important information about expression target genes, eliminating need biopsies. This comprehensive review aims to provide detailed overview current knowledge advancements study cfRNA, focusing on its diverse landscape biological functions, detection methods, diagnostic prognostic various diseases, challenges, future perspectives.

Language: Английский

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Gut microbiota and their derivatives in the progression of colorectal cancer: Mechanisms of action, genome and epigenome contributions

Heliyon, Journal Year: 2024, Volume and Issue: 10(8), P. e29495 - e29495

Published: April 1, 2024

Gut microbiota interacts with host epithelial cells and regulates many physiological functions such as genetics, epigenetics, metabolism of nutrients, immune functions. Dietary factors may also be involved in the etiology colorectal cancer (CRC), especially when an unhealthy diet is consumed excess calorie intake bad practices like smoking or consuming a great deal alcohol. Bacteria including Fusobacterium nucleatum, Enterotoxigenic Bacteroides fragilis (ETBF), Escherichia coli (E. coli) actively participate carcinogenesis CRC. Gastrointestinal tract chronic inflammation immunocompromised patients are at high risk for CRC progression. Further, gut Geno-toxicity by producing toxins colibactin cytolethal distending toxin (CDT) which cause damage to double-stranded DNA. Specific microRNAs can act either tumor suppressors oncogenes depending on cellular environment they expressed. The current review mainly highlights role CRC, mechanisms several carcinogenesis, particular microbes neoplasia.

Language: Английский

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Detection of reproducible liver cancer specific ligand-receptor signaling in blood

Frontiers in Bioinformatics, Journal Year: 2025, Volume and Issue: 4

Published: Jan. 9, 2025

Cell-cell communication mediated by ligand-receptor interactions (LRI) is critical to coordinating diverse biological processes in homeostasis and disease. Lately, our understanding of these has greatly expanded through the inference cellular communication, utilizing RNA extracted from bulk tissue or individual cells. Considering challenge obtaining biopsies for approaches, we considered potential studying cell-free obtained blood. To test feasibility this approach, used BulkSignalR algorithm across 295 samples compared LRI profiles multiple cancer types healthy donors. Interestingly, detected specific reproducible LRIs particularly blood liver patients We found an increase magnitude hepatocyte interactions, notably autocrine patients. Additionally, a robust panel 30 cancer-specific presents bridge linking pathogenesis discernible markers. In summary, approach shows plausibility detecting builds upon transcriptomes.

Language: Английский

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An investigation of plasma cell-free RNA for the detection of colorectal cancer: From transcriptome marker selection to targeted validation

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(8), P. e0308711 - e0308711

Published: Aug. 15, 2024

Regular screening for colorectal cancer (CRC) is critical early detection and long-term survival. Despite the current options available advancements in therapies there will be around 53,000 CRC related deaths this year. There great interest non-invasive alternatives such as plasma cell-free RNA (cfRNA) diagnostic, prognostic, predictive applications. In study, our aim was to identify validate potential cfRNA candidates improve diagnosis. phase 1 (n = 49; 25 controls, 24 cancers), discovery total sequencing performed. Select exons underwent validation 2 73; 35 29 cancers, 9 adenomas) using targeted capture 10,371 probes). 3 57; 30 27 RT-qPCR performed on previously identified 99). were 895 that differentially expressed (325 upregulated, 570 downregulated) among cancers versus controls. phases 3, fewer markers validated than expected independent sets of patients, most which from published literature (FGA, FGB, GPR107, CDH3, RP23AP7). summary, we optimized laboratory processes data analysis strategies can serve methodological framework future studies beyond just scope detection. Additionally, further exploration needed order determine if few study have clinical utility Over time, technologies, analysis, preservation methods at time collection may biological technical reproducibility biomarkers enhance feasibility RNA-based liquid biopsies.

Language: Английский

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Minimal residual disease in colorectal cancer. Tumor-informed versus tumor-agnostic approaches: unraveling the optimal strategy

Annals of Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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Circulating cell-free transcriptomics in cancer

Journal of Lung Pulmonary & Respiratory Research, Journal Year: 2023, Volume and Issue: 10(2), P. 23 - 25

Published: April 12, 2023

Transcriptomics (or functional genomics) is a powerful tool that allow researchers to connect their knowledge of cells, biomarkers, and disease onset, hence providing novel diagnostic therapeutic solutions perspectives. It includes the tempo-spatial distribution, communication interaction key cellular mRNA biomarkers cross-talking networks, role in influencing intracellular extracellular dynamics signaling. Circulating cell-free transcriptomics uses data from plasma transcriptomes, or whole circulating (cfmRNA) content, determine roles functions for biomarker discovery. This short highlights some technologies powering advances field, including current trends innovations, future challenges possibilities—some which were unthinkable few years ago.

Language: Английский

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Decoding cell-type contributions to the cfRNA transcriptomic landscape of liver cancer

Human Genomics, Journal Year: 2023, Volume and Issue: 17(1)

Published: Oct. 5, 2023

Liquid biopsy, particularly cell-free RNA (cfRNA), has emerged as a promising non-invasive diagnostic tool for various diseases, including cancer, due to its accessibility and the wealth of information it provides. A key area interest is composition cellular origin cfRNA in blood alterations transcriptomic landscape during carcinogenesis. Investigating these changes can offer insights into manifestations tissue blood, potentially leading more effective strategies. However, consistency findings across different studies their clinical utility remains be fully elucidated, highlighting need further research this area. In study, we analyzed over 350 samples from four distinct studies, investigating cell type contributions liver cancer. We found that an increase hepatocyte proportions consistent feature most effectively utilized classifying cancer healthy samples. Moreover, our analysis revealed addition hepatocytes, endothelial signatures are also prominent observed changes. By comparing classification performance established markers, demonstrated could distinguish existing markers even enhance when used combination with markers. Our comprehensive cell-type robust effects help classify This especially noteworthy, considering heterogeneous nature datasets etiological distinctions Furthermore, differences results underscore importance integrative comparative approaches future determine robustness findings. study contributes understanding highlights potential deconvolution liquid biopsy.

Language: Английский

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