Lung-gut axis of microbiome alterations following co-exposure to ultrafine carbon black and ozone

Particle and Fibre Toxicology, Journal Year: 2023, Volume and Issue: 20(1)

Published: April 21, 2023

Abstract Background Microbial dysbiosis is a potential mediator of air pollution-induced adverse outcomes. However, systemic comparison the lung and gut microbiome alterations lung-gut axis following pollution exposure scant. In this study, we exposed male C57BL/6J mice to inhaled air, CB (10 mg/m 3 ), O (2 ppm) or + mixture for h/day either one day four consecutive days were euthanized 24 h post last exposure. The quantified by 16 s sequencing. Results Multiple exposures induced an increase in inflammatory cells (neutrophils, eosinophils B lymphocytes), reduced absolute bacterial load lungs increased gut. was more potent as it decreased alpha diversity just after single co-exposure uniquely Clostridiaceae Prevotellaceae lungs. Serum short chain fatty acids (SCFA) (acetate propionate) significantly only co-exposure. A significant SCFA producing families ( Ruminococcaceae , Lachnospiraceae Eubacterium ) also observed multiple exposures. Co-exposure derived metabolite receptors/mediator Gcg, Glp-1r, Cck mRNA expression. Oxidative stress related expression lungs, oxidant levels BALF, serum Conclusion Our study confirms distinct inhalation indicate homeostatic shift counter deleterious impacts environmental on metabolic system.

Language: Английский

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The implication of microbiome in lungs cancer: mechanisms and strategies of cancer growth, diagnosis and therapy

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: March 31, 2024

Available evidence illustrates that microbiome is a promising target for the study of growth, diagnosis and therapy various types cancer. Lung cancer leading cause death worldwide. The relationship microbiota their products with diverse pathologic conditions has been getting large attention. novel research suggests plays an important role in growth progression lung crucial maintaining mucosal immunity synchronizing stability between tolerance inflammation. Alteration identified as critical player negatively impacts patient. Studies suggest healthy essential effective therapy. Various clinical trials are focusing on enhancing treatment efficacy by altering microbiome. regulation will provide innovative strategies maintenance host homeostasis prevention patients. In current review article, we presented latest progress about involvement Furthermore, also assessed therapeutic status management

Language: Английский

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Association between airway microbiota and systemic inflammation markers in non-small cell lung cancer patients

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 28, 2025

Language: Английский

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The Current Roadmap of Lung Cancer Biology, Genomics and Racial Disparity

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3818 - 3818

Published: April 17, 2025

Globally, lung cancer is the most prevalent cause of cancer-related death. There are two large histological groups cancer: small-cell (SCLC) and non-small-cell (NSCLC). Based on histopathological molecular features, adenocarcinoma (ADC) squamous cell carcinoma (SCC) major histologic subtypes NSCLC. Various epidemiological environmental factors linked with an increased risk cancer. However, these show disparities in patients divergent racial ethnic backgrounds. Interestingly, different populations were found to harbor distinct features as evidenced by variations genetic mutation profiles. Moreover, diverse progression patterns identified cancer, which could be crucial improving diagnosis, prognosis, therapeutic planning. In concert a plethora nuclear alterations, mitochondrial alteration, epigenetic reprogramming, microbial dysbiosis, immune alteration signatures have been various types. This review article provides comprehensive overview screening tests treatment strategies for NSCLC SCLC, including surgery, radiation therapy, chemotherapy, targeted therapies, immunotherapies. Through unification aspects, this aspires complete understanding cancer’s genomics, biology, landscapes, disparity seeks understand essential role occurrence treatment.

Language: Английский

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Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 7, 2024

Introduction Lung cancer is the most commonly diagnosed and main cause of death, usually related to cigarette smoking. Furthermore, microbiota people exposed smoke can be modified, making it difficult eliminate opportunistic microorganisms. The leaves Eugenia pyriformis are a by-product fruit production and, date, there have been no studies addressing antiproliferative, anti-inflammatory, antimicrobial activities. Objective Investigate antimicrobial, Nitric Oxide (NO)-production inhibition, antiproliferative activities essential oil from E. its possible effect on treatment prevention damage caused by tobacco. Methods (EO) was obtained hydrodistillation (3 h). Its chemical composition investigated GC-MS. It proposed investigate activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), hepatocellular (HepG2) carcinomas. A non-tumor primary culture pig liver (PLP2) also tested. EO capacity inhibit nitric oxide (NO) evaluated lipopolysaccharide stimulated murine macrophage line. Antibacterial antifungal pathogens were seven strains bacteria eight fungi. Results results indicated presence 23 compounds in oil, majority spathulenol (45.63%) β-caryophyllene (12.72%). Leaf provided 50% inhibition at concentration 92.04 µg mL −1 . demonstrated all lines studied, with GI50 values comprised between 270.86 337.25 showed potential Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) Salmonella Typhimurium ATCC 13311 (MIC 1870 ) fungi Aspergillus versicolor 11730, ochraceus 12066, Penicillium ochrochloron 90288, verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) Trichoderma viride Pers. IAM 5061 (1,400 –1 ). Conclusion add value chain this plant, being option for preventing combating cancer, including cancer.

Language: Английский

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The Complex Role of the Microbiome in Non-Small Cell Lung Cancer Development and Progression

Cells, Journal Year: 2023, Volume and Issue: 12(24), P. 2801 - 2801

Published: Dec. 8, 2023

In recent years, there has been a growing interest in the relationship between microorganisms surrounding environment and cancer cells. While tumor microenvironment predominantly comprises cells, stromal immune emerging research highlights significant contributions of microbial cells to development progression. Although impact gut microbiome on treatment response lung is well established, investigations indicate complex roles microbiota cancer. This article focuses findings human its impacts We delve into characteristics influence development. Additionally, we explore intratumoral microbiome, metabolic interactions how microorganism-produced metabolites can contribute Furthermore, provide comprehensive review current literature implications for metastatic potential this discusses therapeutic modulation establish prevention strategies optimize treatment.

Language: Английский

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Analyzing the characteristics of respiratory microbiota after the placement of an airway stent for malignant central airway obstruction

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(6)

Published: May 15, 2024

Malignant central airway stenosis is treated with stent placement, but post-placement microbial characteristics remain unclear. We studied features in 60 patients post-stent focusing on changes during granulation tissue proliferation. Samples were collected before (

Language: Английский

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Pulmonary microbiota intervention alleviates fine particulate matter-induced lung inflammation in broilers

Journal of Animal Science, Journal Year: 2023, Volume and Issue: 101

Published: Jan. 1, 2023

Fine particulate matter (PM2.5) released during the livestock industry endangers respiratory health of animals. Our previous findings suggested that broilers exposed to PM2.5 exhibited lung inflammation and changes in pulmonary microbiome. Therefore, this study was investigate whether microbiota plays a causal role pathogenesis PM2.5-induced inflammation. We first used antibiotics establish intervention broiler model, which showed significantly reduced total bacterial load lungs without affecting composition or structure. Based on it, 45 AA similar body weight were randomly assigned three groups: control (CON), (PM), (ABX-PM). From 21 d age, ABX-PM group intratracheally instilled with once day for 3 d. Meanwhile, other two groups simultaneously sterile saline. On 24 26 PM suspension induce inflammation, CON The histomorphology, inflammatory cytokines' expression levels, microbiome, microbial growth conditions analyzed determine effect Broilers histological injury, while had normal histomorphology. Furthermore, mRNA levels interleukin-1β, tumor necrosis factor-α, interleukin-6, interleukin-8, toll-like receptor 4 nuclear factor kappa-B. induced significant β diversity structure group. However, no observed Moreover, relative abundance Enterococcus cecorum higher than groups. And bronchoalveolar lavage fluid from promoted E. cecorum, indicating altered microbiota's condition. In conclusion, can affect broilers. alter environment promote dysbiosis, potentially exacerbating

Language: Английский

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Comprehensive sampling of the lung microbiome in early-stage non–small cell lung cancer

JTCVS Open, Journal Year: 2023, Volume and Issue: 17, P. 260 - 268

Published: Nov. 30, 2023

ObjectivesData are scarce on whether the composition of lung microbiome (extending from nasopharynx to peripheral tissue) varies according histology or grade non–small cell cancer. We hypothesized that would vary and cancer.MethodsWe collected naso-oral central lobar (cancer affected, ipsilateral unaffected, contralateral unaffected) bronchoalveolar lavage fluid brushing samples patients with clinical early-stage cancer between July 2018 February 2020 at a single academic center. performed bacterial 16S rRNA sequencing then compared pathologic findings signatures.ResultsSamples were 28 patients. Microbial in affected lobes displayed unique enrichment oropharyngeal species was significantly different unaffected lobes; chronic obstructive pulmonary disease had similar diversity those without (P = .1312). The adenocarcinoma squamous .27). There no differences versus trend toward lower microbial poorly differentiated adenocarcinomas well-differentiated .08).ConclusionsThe microbiota differs same patient. Furthermore, cancers associated diversity. Larger studies will be required confirm these findings.

Language: Английский

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Exploring the potential role of microbiota and metabolites in acute exacerbation of chronic obstructive pulmonary disease

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 16, 2024

The acute exacerbation of chronic obstructive pulmonary disease seriously affects the respiratory system function and quality life patients. This study employed 16S rRNA sequencing metabolomics techniques to analyze microbiota serum metabolites COPD AECOPD results showed that microbial diversity in tract patients was significantly lower than patients, relative abundance Bacteroidetes,

Language: Английский

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