Astragalus membranaceus and Salvia miltiorrhiza ameliorate diabetic kidney disease via the “gut-kidney axis”

Phytomedicine, Journal Year: 2023, Volume and Issue: 121, P. 155129 - 155129

Published: Sept. 29, 2023

Language: Английский

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Dynamic changes of gut microbiota composition during the intervention of apple polyphenols extract to alleviate high-carbohydrate-diet induced body weight gain

Food Bioscience, Journal Year: 2024, Volume and Issue: 60, P. 104272 - 104272

Published: May 10, 2024

Language: Английский

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Integrating 16S rDNA and metabolomics to uncover the therapeutic mechanism of electroacupuncture in type 2 diabetic rats

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 6, 2025

This study aimed to investigate the impact of electroacupuncture (EA) on blood glucose levels, gut microbiota, short-chain fatty acids (SCFAs), and glucagon-like peptide-1 (GLP-1) in a rat model type 2 diabetes mellitus (T2DM). Forty Sprague-Dawley (SD) rats were randomly assigned five groups (n = 8/group) using random number table: normal control, T2DM model, (EA), EA + antibiotics (EA A), (A). The received standard diet saline gavage, while other fed high-fat emulsion. A additional antibiotic solution gavage. normal, immobilized restrained for 30 min, six times per week, 4 weeks. treatment at specific acupoints parameters continuous waves 10 Hz 1-2 mA. During intervention, water food consumption, body weight, fasting (FBG), oral tolerance test (OGTT) monitored. Pancreatic tissue was examined hematoxylin eosin (H&E) staining. Fecal microbial communities analyzed by 16S rDNA sequencing, (SCFAs) measured gas chromatography-mass spectrometry (GC-MS). Serum levels insulin (FINS), glycated hemoglobin (HbA1c), determined enzyme-linked immunosorbent assay (ELISA). significantly improved daily intake, weight (p < 0.01). also reduced FBG, area under curve OGTT, FINS, homeostasis assessment resistance (HOMA-IR) 0.05). ELISA results showed lower concentration HbA1c group overall morphology pancreatic islets, increased β-cell nuclei, alleviated hypertrophy. abundance operational taxonomic units (OTUs) than 0.05), upregulated Shannon, Chao1, Ace indices concentrations acetic acid, butyric GLP-1 Correlation analysis revealed negative associations between Lactobacillaceae (R -0.81, p 0.015) Lactobacillus -0.759, 0.029) with FBG. Peptostreptococcaceae Romboutsia negatively correlated 0.015), Enterobacteriaceae positively OGTT 0.762, 0.028). acid 0.487, 0.001), 0.586, 0.000), isovaleric 0.374, 0.017), valeric 0.535, caproic 0.371, 0.018). Antibiotics disrupted intestinal microbiota structure weakened therapeutic effects EA. effectively metabolism rats. hypoglycemic associated regulation SCFAs, GLP-1.

Language: Английский

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Bavachin ameliorates HFD-induced obesity through enhancing gut microbiota-regulated adipose thermogenesis

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106212 - 106212

Published: Feb. 1, 2025

Language: Английский

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Gut microbiota promote the propagation of pathologic α-syn from gut to brain in a gut-originated mouse model of Parkinson’s disease

Brain Behavior and Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Morel (Morchella spp.) intake alters gut microbial community and short-chain fatty acid profiles in mice

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: Sept. 22, 2023

Morels (Morchella spp.) are highly nutritious and consumed as both edible mushrooms traditional Chinese medicine. This study aimed to investigate the effects of dietary supplementation with morel on gut bacterial microbiota short-chain fatty acids (SCFAs) profiles in healthy mice. Healthy mice were randomly assigned five groups: a control group (0% morel) four intervention groups supplemented different levels (5% for M5, 10% M10, 15% M15, 20% M20) over period 4 weeks. Fecal samples collected at end experiment characterize assess SCFAs levels. The significantly altered community composition, increasing Bacteroides, Lachnospiraceae NK4A136 Parabacteroides, while decreasing Staphylococcus Firmicutes Bacteroidetes ratio (F/B ratio). Moreover, increased intake was associated weight loss. All content upregulated morel-intervention groups. Potential SCFAs-producing taxa identified by regression analysis distributed families Muribaculaceae, Lachnospiraceae, genera Jeotgalicoccus, Gemella, Odoribacter, Tyzzerella 3 Ruminococcaceae UCG-014. functional categories involved SCFAs-production or loss may contain enzymes such beta-glucosidase (K05349), beta-galactosidase (K01190), hexosaminidase (K12373) after intervention. exploration impact metabolites contributes development prebiotics improving health reducing obesity.

Language: Английский

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Harnessing Prebiotics to Improve Type 2 Diabetes Outcomes

Nutrients, Journal Year: 2024, Volume and Issue: 16(20), P. 3447 - 3447

Published: Oct. 11, 2024

The gut microbiota, a complex ecosystem of microorganisms in the human gastrointestinal tract (GI), plays crucial role maintaining metabolic health and influencing disease susceptibility. Dysbiosis, or an imbalance has been linked to development type 2 diabetes mellitus (T2DM) through mechanisms such as reduced glucose tolerance increased insulin resistance. A balanced eubiosis, is associated with improved metabolism sensitivity, potentially reducing risk diabetes-related complications. Various strategies, including use prebiotics like inulin, fructooligosaccharides, galactooligosaccharides, resistant starch, pectic oligosaccharides, polyphenols, β-glucan, Dendrobium officinale have shown improve microbial composition support glycemic control T2DM patients. These can directly impact blood sugar levels while promoting growth beneficial bacteria, thus enhancing control. Studies that patients often exhibit decrease butyrate-producing Roseburia Faecalibacterium, increase harmful Escherichia Prevotella. This review aims explore effects different on T2DM, their microbiota composition, potential for personalized dietary interventions optimize management overall outcomes.

Language: Английский

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Ji-Ni-De-Xie ameliorates type 2 diabetes mellitus by modulating the bile acids metabolism and FXR/FGF15 signaling pathway

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: May 21, 2024

Introduction: Ji-Ni-De-Xie (JNDX) is a traditional herbal preparation in China. It widely used to treat type 2 diabetes mellitus (T2DM) Tibetan medicine system. However, its antidiabetic mechanisms have not been elucidated. The aim of this study elucidate the underlying mechanism JNDX on bile acids (BAs) metabolism and FXR/FGF15 signaling pathway T2DM rats. Methods: High-performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QQQ-MS) UPLC-Q-Exactive Orbitrap MS technology were identify constituents JNDX. High-fat diet (HFD) combined with streptozotocin (45 mg∙kg −1 ) (STZ) was establish rat model, levels fasting blood-glucose (FBG), glycosylated serum protein (GSP), homeostasis model assessment insulin resistance (HOMA-IR), LPS, TNF-α, IL-1β, IL-6, TG, TC, LDL-C, HDL-C, sensitivity index (ISI) measured evaluate anti-diabetic activity In addition, metagenomic analysis performed detect changes gut microbiota. metabolic profile BAs analyzed by HPLC-QQQ-MS. Moreover, mRNA expressions FXR FGF15 colon CYP7A1 liver rats western blot RT-qPCR. Results: A total 12 identified HPLC-QQQ-MS Furthermore, 45 chemical components from via technology, including 22 prototype 23 metabolites. Using we found that (0.083, 0.165 0.33 g/kg) reduced FBG, GSP, HOMA-IR, increased ISI HDL-C Metagenomic results demonstrated treatment effectively improved microbiota dysbiosis, altering some bacteria (e.g., Streptococcus Bacteroides associated metabolism. Additionally, disorder rats, especially significantly increasing cholic acid (CA) decreasing ursodeoxycholic (UDCA) levels. increased, while expression markedly inhibited Discussion: can improve resistance, hyperglycemia, hyperlipidemia, inflammation related regulation activation pathway.

Language: Английский

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Integrated analysis of gut microbiome and its metabolites in ACE2-knockout and ACE2-overexpressed mice

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: July 17, 2024

Background Aberrant activation of the classic renin–angiotensin system (RAS) and intestinal micro dysbiosis adversely affect insulin resistance (IR), dyslipidemia, other metabolic syndrome markers. However, action angiotensin-converting enzyme 2 (ACE2) gut health in systemic homeostasis vary, their interaction is not completely understood. Methods We adopted a combinatory approach metabolomics fecal 16S rRNA analysis to investigate microbiota metabolite two different mouse models, ACE2 knockout ( KO) mice ACE2-overexpressing obese mice. Results gene sequencing revealed that influences microbial community composition function, KO had increased Deferribacteres , Alcaligenaceae Parasutterella Catenibacterium Anaerotruncus with decreased short-chain fatty acid (SCFA)-producing bacteria Marvinbryantia Alistipes ). In contrast, ACE2-overexpressed exhibited anti-inflammatory probiotic Oscillospiraceae Marinifilaceae Bifidobacteriaceae ) SCFA-producing microbes Rikenellaceae, Muribaculaceae Ruminococcaceae Odoribacter Firmicutes/Bacteroidetes Lactobacillaceae Erysipelotrichaceae Lachnospiraceae . Metabolome indicated differential metabolites ACE2-overexpression mice, especially glucolipid metabolism-related compounds. Furthermore, correlation between showed dynamic mutual influence affecting host health. Conclusion Our study confirms for first time significant association status microbiome metabolome profiles, providing novel mechanism positive effect on energy homeostasis.

Language: Английский

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Pectin-derived oligogalacturonic acids ameliorate high-fat diet-induced obesity in mice by regulating gut microbiota and inflammation

Journal of Functional Foods, Journal Year: 2023, Volume and Issue: 112, P. 105928 - 105928

Published: Dec. 2, 2023

Pectic oligosaccharides have been proposed as a novel prebiotic to prevent obesity and associated with metabolic disorders by regulating gut microbiota, but their various probiotic functions in the depended on complex heterogeneous sugar composition. This study aimed obtain specific oligogalacturonic acids (POAS) from pectin investigate effects of POAS high-fat diet-induced mice. The results showed that improved body weight gain, serum lipids hepatic steatosis obese notably enhanced intestinal barrier function, reduced inflammatory response regulated expression key genes lipid synthesis metabolism. Besides, also promoted short-chain fatty (SCFAs) production. Furthermore, alleviated microbiota dysbiosis caused HFD significantly increasing beneficial such Akkermansia Lactobacillus. Spearman's correlation analysis indicated these bacteria were strongly correlated obesity-related parameters. We conclude is potential treat related complications.

Language: Английский

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