Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway

Acta Biochimica et Biophysica Sinica, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Inflammatory bowel disease (IBD) is a chronic inflammatory characterized by intestinal barrier dysfunction, synergistic effects and excessive tissue injury. Gallic acid (GA) renowned for its remarkable biological activity, encompassing anti-inflammatory antioxidant properties. However, the underlying mechanisms which GA protects against inflammation have not been fully elucidated. The aim of this study to investigate effect on lipopolysaccharide (LPS)-stimulated human colon carcinoma cell line (Caco-2) explore molecular mechanism involved. Our findings demonstrate that 5 μg/mL restores downregulation mRNA protein levels Claudin-1, Occludin, ZO-1 decreases expressions factors such as IL-6, IL-1β TNF-α induced LPS. In addition, exhibits protective reducing LPS-enhanced early late apoptotic ratios, downregulating pro-apoptotic (

Language: Английский

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Anti‐inflammatory and anti‐hyperalgesic effects induced by an aqueous aged black garlic extract in rodent models of ulcerative colitis and colitis‐associated visceral pain

Phytotherapy Research, Journal Year: 2024, Volume and Issue: 38(8), P. 4177 - 4188

Published: June 24, 2024

Abstract Inflammatory bowel disease (IBD) is a morbid condition characterized by relapsing–remitting inflammation of the colon, accompanied persistent gut dysmotility and abdominal pain. Different reports demonstrated biological activities aged black garlic (ABG), including anti‐inflammatory antioxidant effects. We aimed to investigate beneficial effects exerted ABGE on colon using ex vivo in experimental models. investigated an ABG water extract (ABGE) rat specimens exposed E. coli lipopolysaccharide (LPS), known model ulcerative colitis. determined gene expression various biomarkers involved inflammation, interleukin (IL)‐1β, IL‐6, nuclear factor‐kB (NF‐kB), tumor necrosis factor (TNF)‐α. Moreover, we studied acute visceral pain associated with colitis induced 2,4‐di‐nitrobenzene sulfonic acid (DNBS) injection rats. suppressed LPS‐induced IL‐1β, NF‐kB, TNF‐α. In addition, administration (0.03–1 g kg −1 ) dose‐dependently relieved post‐inflammatory pain, higher dose (1 able significantly reduce both behavioral nociceptive response entity contraction (assessed electromyography) colorectal distension after DNBS‐treated Present findings showed that could represent potential strategy for treatment colitis‐associated inflammatory process The be related pattern polyphenolic composition, particular regard gallic catechin.

Language: Английский

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Gallic Acid-Modified Polyethylenimine–Polypropylene Carbonate–Polyethylenimine Nanoparticles: Synthesis, Characterization, and Anti-Periodontitis Evaluation

ACS Omega, Journal Year: 2024, Volume and Issue: 9(12), P. 14475 - 14488

Published: March 15, 2024

The aim of the research was to develop novel gallic acid (GA)-modified amphiphilic nanoparticles polyethylenimine (PEI)–polypropylene carbonate (PPC)–PEI (PEPE) and comprehensively assess its properties as an antiperiodontitis nanoparticle targeting Toll-like receptor (TLR). first step is evaluate binding potential GA core trigger receptors TLR2 TLR4/MD2 for periodontitis using molecular docking techniques. Following this, we conducted NMR, transmission electron microscopy, dynamic light scattering analyses on synthesized PEPE nanoparticles. As final step, investigated synthetic results in vitro GA-PEPE investigation revealed that exhibits targeted complex. Furthermore, successfully developed 91.19 nm positively charged Spectroscopic analysis indicated successful synthesis GA-modified PEPE. Additionally, CCK8 demonstrated modification significantly reduced biotoxicity assessment illustrated 6.25 μM expression pro-inflammatory factors TNF-α, IL-1β, IL-6. nanoparticles, with their TLR capabilities, were found possess excellent biocompatibility properties. will provide highly innovative input into development anti-

Language: Английский

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Therapeutic Implications of Phenolic Acids for Ameliorating Inflammatory Bowel Disease

Nutrients, Journal Year: 2024, Volume and Issue: 16(9), P. 1347 - 1347

Published: April 29, 2024

Inflammatory bowel disease (IBD) is a chronic inflammatory intestinal disorder, and its complex etiology makes prevention treatment challenging. Research on new drugs strategies currently focal point. Phenolic acids are widely present in plant-based diets have demonstrated the potential to alleviate colitis due their powerful antioxidant anti-inflammatory properties. In this review, we provide an overview of structures main dietary sources phenolic acids, encompassing benzoic acid cinnamic acid. Additionally, explore as nutritional therapy for preventing treating IBD. animal cell experiments, effectively IBD induced by drug exposure or genetic defects. The mechanisms include improving mucosal barrier function, reducing oxidative stress, inhibiting excessive activation immune response, regulating balance microbiota. Our observation points towards need additional basic clinical investigations derivatives novel therapeutic agents

Language: Английский

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Changyanning tablet alleviates Crohn's disease by inhibiting GPX4-mediated ferroptosis

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119415 - 119415

Published: Jan. 1, 2025

Language: Английский

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Regulation of pyroptosis by natural products in ulcerative colitis: mechanisms and therapeutic potential

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

Ulcerative colitis (UC), a chronic inflammatory bowel disease, is driven by dysregulated immune responses and persistent intestinal inflammation. Pyroptosis, caspase/gasdermin-mediated cell death that exacerbates mucosal damage through excessive cytokine release epithelial barrier disruption. Although pyroptosis considered to be key mechanism in the pathogenesis of UC, systematic assessment role natural products targeting pathway remains critical research gap. The purpose this review investigate regulatory effects on UC elucidate mechanisms action potential therapeutic effects. Key findings highlight polyphenols (e.g., resveratrol), flavonoids Quercetin), terpenoids as promising agents inhibit NLRP3 inflammasome activation, suppress gasdermin D cleavage, restore integrity, thereby reducing pro-inflammatory preclinical models. Current evidence shows enhanced efficacy safety when these compounds are combined with standard therapies, but clinical translation requires overcoming three barriers: limited human trial data, uncharacterized polypharmacology, suboptimal pharmacokinetics needing formulation refinement. Future should prioritize standardized animal-to-human translational models, mechanistic studies synergistic pathways, rigorous validation harness full pyroptosis-targeted therapies.

Language: Английский

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Rosa Roxburghii Tratt Fruit Extract Prevents Dss-Induced Ulcerative Colitis in Mice by Modulating the Gut Microbiota and the IL-17 Signaling Pathway

Nutrients, Journal Year: 2023, Volume and Issue: 15(21), P. 4560 - 4560

Published: Oct. 27, 2023

Ulcerative colitis (UC) is a non-specific inflammatory bowel illness characterized by intestinal mucosal barrier degradation, inflammation, oxidative damage, and gut microbiota imbalances. Rosa roxburghii Tratt Fruit extract (RRTE) was extracted from fruit, exhibiting an excellent prevention effect against UC; RRTE could prevent the damage of DSS-induced human normal colonic epithelial (NCM 460) cells, especially in cell viability morphology, damage. Additionally, UC mice, limit increasing expression tight junction proteins mucin, reducing inflammation colon tissue. More importantly, can increase abundance beneficial bacteria to regulate such as Ruminococcus, Turicibacter, Parabacteroides, reduce harmful Staphylococcus Shigella. Furthermore, transcriptomics findings point out that on be attributed modulation responses IL-17 TNF signaling pathways. The qPCR results confirm did involve regulation several genes pathway. In conclusion, both vitro vivo.

Language: Английский

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Application of Chinese Medicine in Treatment of Ulcerative Colitis and Elucidation of Relevant Mechanisms

Chinese Journal of Integrative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

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Sea buckthorn leaves and gallic acid inhibit C48/80-induced pseudo-allergic reaction via the PLC/IP3 signaling pathway both in vitro and in vivo

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 154, P. 114563 - 114563

Published: April 2, 2025

Language: Английский

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Terminalia bellirica Fruit Extract Alleviates DSS-Induced Ulcerative Colitis by Regulating Gut Microbiota, Inflammatory Mediators, and Cytokines

Molecules, Journal Year: 2023, Volume and Issue: 28(15), P. 5783 - 5783

Published: July 31, 2023

Ulcerative colitis (UC) is a chronic inflammatory disease significantly impacting patients’ lives. This study aimed to elucidate the alleviating effect of ethyl acetate extract (TBEA) from Terminalia bellirica fruit on UC and explore its mechanism. TBEA was fraction with best anti-inflammatory activity screened using in vitro assays, HPLC initially characterized composition. The mice model ulcerative established after free drinking 2.5% dextran sulfate sodium for six days, experimental group treated 50 mg/kg 100 seven days. We found that alleviated symptoms mice, including physiologically significant reduction index pathological damage colonic tissue. dramatically slowed down oxidative stress process as evidenced by decreasing myeloperoxidase malondialdehyde activities increasing glutathione catalase levels reducing concentrations IL-6, IL-1β, TNF-α, NO well regulating key proteins IL-6/JAK2/STAT3 pathway. Meanwhile, maintained intestinal homeostasis flora structure. Our provides new ideas developing into drug treat UC.

Language: Английский

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