Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)
Published: Sept. 28, 2024
Language: Английский
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: May 21, 2024
Impressive advances have been made to replicate human physiology in vitro over the last few years due growth of organ-on-chip (OoC) field both industrial and academic settings. OoCs are a type microphysiological system (MPS) that imitates functional dynamic aspects native organ biology on microfluidic device. Organoids organotypic models, ranging their complexity from simple single-cell complex multi-cell constructs, being incorporated into OoC devices better mimic physiology. technology has now progressed stage at which it received official recognition by Food Drug Administration (FDA) for use as an alternative standard procedures drug development, such animal studies traditional assays. However, area is still lagging behind incorporation immune system, critical element required investigate health disease. In this review, we summarise progress integrate immunology various systems, specifically focusing models related barriers lymphoid organs. These utilise either commercially available or custom-made. This review explores difference between innate adaptive cells role modelling organ-specific diseases OoCs. Immunocompetent multi-OoC also highlighted extent they recapitulate systemic discussed. Together, aim describe current state immune-OoCs, limitations future perspectives needed improve field.
Language: Английский
Citations
7
ACS Omega, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 10, 2024
Amyotrophic lateral sclerosis (ALS) is a progressive and devastating neurodegenerative disorder characterized by the loss of upper lower motor neurons, resulting in debilitating muscle weakness atrophy. Currently, there are no effective treatments available for ALS, posing significant challenges managing disease that affects approximately two individuals per 100,000 people annually. To address urgent need ALS treatments, we conducted drug repurposing study using combination bioinformatics tools molecular docking techniques. We analyzed sporadic ALS-related genes from GEO database identified key signaling pathways involved pathogenesis through pathway analysis DAVID. Subsequently, utilized Clue Connectivity Map to identify potential candidates performed AutoDock Vina evaluate binding affinity short-listed drugs genes. Our Cefaclor, Diphenidol, Flubendazole, Fluticasone, Lestaurtinib, Nadolol, Phenamil, Temozolomide, Tolterodine as treatment. Notably, Lestaurtinib demonstrated high toward multiple proteins, suggesting its broad-spectrum therapeutic agent ALS. Additionally, revealed NOS3 gene interacts with all drugs, possible involvement mechanisms underlying these Overall, our provides systematic framework identifying therapy highlights promising strategy discovering new therapies diseases.
Language: Английский
Citations
6
Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)
Published: April 10, 2024
Acute hypoxic proximal tubule (PT) injury and subsequent maladaptive repair present high mortality increased risk of acute kidney (AKI) - chronic disease (CKD) transition. Human bone marrow mesenchymal stem cell-derived exosomes (hBMMSC-Exos) as potential cell therapeutics can be translated into clinics if drawbacks on safety efficacy are clarified. Here, we determined the real-time effective dose treatment window allogeneic hBMMSC-Exos, evaluated their performance structural functional integrity 3D microfluidic PT platform.
Language: Английский
Citations
5
Published: Jan. 1, 2025
Language: Английский
Citations
0
ACS Omega, Journal Year: 2025, Volume and Issue: 10(10), P. 9869 - 9889
Published: March 10, 2025
The limitation of animal models to imitate a therapeutic response in humans is key problem that challenges their use fundamental research. Organ-on-a-chip (OOC) devices, also called microphysiological systems (MPS), are devices containing lining living cells grown under dynamic flow recapitulate the important features human physiology and pathophysiology with high precision. Recent advances microfabrication tissue engineering techniques have led wide adoption OOC next-generation experimental platforms. This review presents comprehensive analysis systems, categorizing them by types (single-pass multipass), operational mechanisms (pumpless pump-driven), configurations (single-organ multiorgan systems), along respective advantages limitations. Furthermore, it explores integration qualitative quantitative assay techniques, providing comparative evaluation without sensor integration. aims fill essential knowledge gaps, driving progress development paving way for breakthroughs biomedical research, pharmaceutical innovation, engineering.
Language: Английский
Citations
0
Assay and Drug Development Technologies, Journal Year: 2025, Volume and Issue: unknown
Published: March 18, 2025
Language: Английский
Citations
0
Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101323 - 101323
Published: April 1, 2025
Language: Английский
Citations
0
Nanotheranostics, Journal Year: 2024, Volume and Issue: 8(3), P. 380 - 400
Published: Jan. 1, 2024
Cancer is a multifactorial disease produced by mutations in the oncogenes and tumor suppressor genes, which result uncontrolled cell proliferation resistance to death.Cancer progresses due escape of altered cells from immune monitoring, facilitated tumor's mutual interaction with its microenvironment.Understanding mechanisms involved surveillance evasion significance microenvironment might thus aid developing improved therapies.Although vivo models are commonly utilized, they could be better for time, cost, ethical concerns.As result, it critical replicate an model recreate cellular tissue-level functionalities.A 3D culture, gives architecture similar that found vivo, appropriate model.Furthermore, numerous types can cocultured, establishing interactions between TME cells.Moreover, microfluidics perfusion provide precision flow rates, simulating tissue/organ function.Immunotherapy used perfused culture technique help develop successful therapeutics.Immunotherapy employing nano delivery target spot silence responsible ensuring treatment effectiveness while minimizing adverse effects.This study focuses on importance understanding pathophysiology tumors TME, function drug resistance, progression, development advanced anticancer therapies high-throughput screening.
Language: Английский
Citations
3
ChemBioChem, Journal Year: 2024, Volume and Issue: 25(23)
Published: Aug. 26, 2024
Abstract Organ‐On‐a‐Chip (OOC) is a multichannel 3D‐microfluidic cell‐culture system incorporated in chip that simulates the behavior of an organ. This technology relies on multidisciplinary science benefits from and contributes progress many fields including microbiology, microfluidics, biomaterials, bioengineering. review article summarizes achievements various organ‐on‐chip technologies. It highlights significant advantages this terms reducing animal testing providing personalized medical responses. In addition, paper demonstrates how OOC becoming promising powerful tool pharmaceutical research to combat diseases. predicts not only effects drugs target organs but also, using body‐on‐a‐chip systems, it may provide insights into side drug delivery other organs. Likewise, models used for construction organ‐on‐a‐chip devices are investigated along with design materials microfluidic devices. For each OOC, integrated monitoring within chips (e. g., sensors biosensors) discussed. We also discuss evolution FDA regulations potential near future integrating OOCs protocols support reduce need failure rates preclinical clinical studies.
Language: Английский
Citations
3
Toxicology Letters, Journal Year: 2024, Volume and Issue: 393, P. 78 - 83
Published: Feb. 3, 2024
Language: Английский
Citations
2