Targeting TXNIP for neuroprotection: A novel approach to reducing inflammation and promoting recovery in ischemic stroke DOI Creative Commons

Chongxin He,

Yong Bao,

Yong Xu

et al.

Biomolecules and Biomedicine, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Ischemic stroke often results in high mortality and significant disability. Current research primarily focuses on understanding neuroinflammation cell death following a to identify novel therapeutic targets. This study investigates the endothelial cell-specific role of Thioredoxin interacting protein (TXNIP) ischemic its underlying molecular mechanisms both vitro vivo. By targeting cells, we aim determine how TXNIP knockdown promotes neuroprotection, enhances angiogenesis, reduces inflammation post-stroke. In vitro, an oxygen-glucose deprivation (OGD) model using bEnd.3 cells simulated conditions. Cellular injury was evaluated through proliferation angiogenesis assays, while dual immunofluorescence staining assessed ZO-1 CD31 expression. Western blotting measured levels TXNIP, nucleotide-binding oligomerization domain-like receptor 3 (NLRP3), ASC, pro-caspase-1, interleukin-1β (IL-1β). vivo, middle cerebral artery occlusion (MCAO) mouse employed mimic stroke. Brain triphenyl tetrazolium chloride (TTC) Nissl staining, changes markers were via blot analysis. promoted reduced inflammation, decreased fluorescence intensity. also reversed OGD-induced upregulation NLRP3, IL-1β. improved neurological recovery, reflected by lower Longa scores, increased body presence, infarct size. These findings suggest that mitigates damage provides valuable insights into potential strategies for treatment.

Language: Английский

Integrating hydrogels manipulate ECM deposition after spinal cord injury for specific neural reconnections via neuronal relays DOI Creative Commons
Zan Tan, Longyou Xiao, Junwu Ma

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(27)

Published: July 3, 2024

A bioinspired hydrogel composed of hyaluronic acid-

Language: Английский

Citations

20
Novel Multi-Antioxidant Approach for Ischemic Stroke Therapy Targeting the Role of Oxidative Stress DOI Creative Commons
Camilo Briones-Valdivieso,

Felipe Briones,

Sofía Orellana-Urzúa

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 501 - 501

Published: Feb. 23, 2024

Stroke is a major contributor to global mortality and disability. While reperfusion essential for preventing neuronal death in the penumbra, it also triggers cerebral ischemia-reperfusion injury, paradoxical injury primarily caused by oxidative stress, inflammation, blood–brain barrier disruption. An burst inflicts marked cellular damage, ranging from alterations mitochondrial function lipid peroxidation activation of intricate signalling pathways that can even lead cell death. Thus, given pivotal role stress mechanisms reinforcement antioxidant defence system has been proposed as protective approach. Although this strategy proven be successful experimental models, its translation into clinical practice yielded inconsistent results. However, should considered availability numerous molecules with wide range chemical properties affect extent injury; several groups molecules, including polyphenols, carotenoids, vitamins, among other compounds, mitigate damage intervening multiple at various stages. Multiple trials have previously conducted evaluate these using melatonin, acetyl-L-carnitine, chrysanthemum extract, edaravone dexborneol, saffron, coenzyme Q10, oleoylethanolamide, treatments. Therefore, multi-antioxidant therapy emerges promising novel therapeutic option due potential synergistic effect provided simultaneous roles individual compounds.

Language: Английский

Citations

14
Recent advances in the treatment of gout with NLRP3 inflammasome inhibitors DOI

Ye Tian,

Xiaofang He,

Ruping Li

et al.

Bioorganic & Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 112, P. 117874 - 117874

Published: Aug. 16, 2024

Language: Английский

Citations

5
Pharmacological and therapeutic effects of natural products on liver regeneration-a comprehensive research DOI Creative Commons
Chang Tian, Yuhan Wang,

Ran Wang

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: May 6, 2025

Abstract Liver regeneration (LR) refers to the physiological process by which hepatocytes undergo cellular proliferation restore structure and function of liver following significant hepatocyte loss due injury or partial hepatectomy (PH). While possesses a remarkable regenerative capacity, this is tightly regulated ensure appropriate cessation once homeostasis reestablished. Various strategies, including technological interventions pharmacological agents, have been explored enhance LR. Among these, natural products emerged as promising candidates for promoting For instance, quercetin, compound, has shown LR PH maintaining redox stimulating proliferation. However, present certain limitations, such poor solubility low bioavailability, may hinder their clinical application. Modifications in formulation mode administration demonstrated potential overcoming these challenges optimizing effects. Recent advancements research further highlighted growing relevance products, traditional Chinese medicine (TCM), context Despite progress, comprehensive systematic review roles, mechanisms, therapeutic remains lacking. This aims bridge gap summarizing with promote Drawing on data from PubMed, Web Science, CNKI databases, it elucidates effects regulatory providing valuable reference future application field

Language: Английский

Citations

0
Curcumin nanoparticles in heat stroke management DOI Creative Commons

Fei Guo,

Yizhan Wu,

Jiangwei Liu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 12, 2024

Language: Английский

Citations

1
Gut microbiome and inflammation in cardiovascular drug response: trends in therapeutic success and commercial focus DOI
Firoz Anwar, Fahad A. Al‐Abbasi,

Omar A. Al-Bar

et al.

Inflammopharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 2, 2024

Language: Английский

Citations

1
Pdgfrβ Participates in the Stat3-Mediated Regulation of Pd-L1 Expression in Colorectal Cancer DOI
Yuan Zhou,

Fengchang Huang,

Di Ran

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Network pharmacology and in vivo experimental studies reveal the protective effects of 6-hydroxygenistein against hypobaric hypoxia-induced brain injury DOI Creative Commons

Zhiqun Shi,

Jie Zhang,

Huiping Ma

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e36241 - e36241

Published: Aug. 1, 2024

Hypobaric hypoxia-induced brain injury (HHBI) is a progressive neurodegenerative disease that has still not been effectively treated. There are several different mechanisms involved in HHBI. Among them, oxidative stress and inflammation response predominate. 6-hydroxygenistein (4',5,6,7-tetrahydroxyisoflavone, 6-OHG) hydroxylated derivative of genistein with excellent antioxidant activity, however, the protective effects underlying against HHBI have clarified. In present study, we aimed to explore action 6-OHG on using network pharmacology experimental validation. Network analysis revealed 186 candidate targets through intersection related genes HHBI, which were mainly enriched response. Moreover, key namely Nrf2 NF-κB, screened found be closely Subsequent

Language: Английский

Citations

0
Targeting TXNIP for neuroprotection: A novel approach to reducing inflammation and promoting recovery in ischemic stroke DOI Creative Commons

Chongxin He,

Yong Bao,

Yong Xu

et al.

Biomolecules and Biomedicine, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 13, 2024

Ischemic stroke often results in high mortality and significant disability. Current research primarily focuses on understanding neuroinflammation cell death following a to identify novel therapeutic targets. This study investigates the endothelial cell-specific role of Thioredoxin interacting protein (TXNIP) ischemic its underlying molecular mechanisms both vitro vivo. By targeting cells, we aim determine how TXNIP knockdown promotes neuroprotection, enhances angiogenesis, reduces inflammation post-stroke. In vitro, an oxygen-glucose deprivation (OGD) model using bEnd.3 cells simulated conditions. Cellular injury was evaluated through proliferation angiogenesis assays, while dual immunofluorescence staining assessed ZO-1 CD31 expression. Western blotting measured levels TXNIP, nucleotide-binding oligomerization domain-like receptor 3 (NLRP3), ASC, pro-caspase-1, interleukin-1β (IL-1β). vivo, middle cerebral artery occlusion (MCAO) mouse employed mimic stroke. Brain triphenyl tetrazolium chloride (TTC) Nissl staining, changes markers were via blot analysis. promoted reduced inflammation, decreased fluorescence intensity. also reversed OGD-induced upregulation NLRP3, IL-1β. improved neurological recovery, reflected by lower Longa scores, increased body presence, infarct size. These findings suggest that mitigates damage provides valuable insights into potential strategies for treatment.

Language: Английский

Citations

0