Separation Science Plus,
Journal Year:
2024,
Volume and Issue:
8(1)
Published: Dec. 27, 2024
ABSTRACT
To
further
improve
the
dilemma
facing
clinical
application
of
doxorubicin
(DOX),
we
designed
a
cathepsin
B
sensitive
DOX
prodrug
albumin
nanoparticle
(NP).
evaluate
pharmacokinetics
NPs
(C16‐GFLG‐PAB‐DOX
NPs),
developed
and
validated
liquid
chromatography‐tandem
mass
spectrometry
method
for
same
time
analysis
its
fatty
acid
(C16‐GFLG‐PAB‐DOX)
in
rat
plasma.
The
uses
precipitated
protein
to
extract
analytes
from
on
selectivity,
linearity
(
r
≥
0.992),
precision
(relative
standard
deviation:
3.5%–9.5%),
accuracy
error:
−2.9%
3.5%),
extraction
recovery
(80.2%
90.4%),
matrix
effect
(87.8%
96.6%),
stability
different
conditions
were
satisfied.
Therefore,
pharmacokinetic
behavior
C16‐GFLG‐PAB‐DOX
rats
was
investigated
using
this
analytical
approach.
outcome
study
illustrated
that
are
promising
alleviate
problems
associated
with
applications.
International Journal of Nanomedicine,
Journal Year:
2025,
Volume and Issue:
Volume 20, P. 4235 - 4255
Published: April 1, 2025
α-Solanine
(α-Sol)
shows
promise
for
pancreatic
cancer
(PC)
treatment
by
inhibiting
PC
cell
proliferation,
migration,
and
invasion.
However,
its
clinical
application
is
hindered
poor
tumor
targeting,
significant
toxicity,
undesirable
pharmacokinetics.
To
address
these
issues,
this
study
developed
a
nanoparticle
delivery
system
(PBSO
NPs)
using
bovine
serum
albumin
as
carrier,
with
polyallylamine
hydrochloride
surface
modification
to
enhance
α-Sol
delivery.
PBSO
NPs
were
characterized
transmission
electron
microscopy,
dynamic
light
scattering,
size
analyzers,
Fourier-transform
infrared
spectroscopy.
Their
in
vitro
drug
release
profile
cellular
uptake
capabilities
evaluated.
Furthermore,
experiments
conducted
mouse
cells
(Panc02)
investigate
the
effects
of
on
Panc02
viability,
invasion,
apoptosis.
Additionally,
xenograft
model
was
established
vivo
explore
impact
growth.
This
successfully
favorable
morphology
physiological
stability,
capable
enhancing
uptake.
In
demonstrated
that
significantly
inhibited
invasion
while
promoting
Moreover,
enhanced
inhibitory
cells.
further
confirmed
improved
therapeutic
efficacy
against
partially
reducing
toxicity.
exhibited
good
biocompatibility.
apoptosis,
thereby
suppressing
progression
PC.
provides
promising
strategy
treatment.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Nov. 14, 2024
RNA
therapeutics,
such
as
mRNA,
siRNA,
and
CRISPR–Cas9,
present
exciting
avenues
for
treating
diverse
diseases.
However,
their
potential
is
commonly
hindered
by
vulnerability
to
degradation
poor
cellular
uptake,
requiring
effective
delivery
systems.
Lipid
nanoparticles
(LNPs)
have
emerged
a
leading
choice
in
vivo
delivery,
offering
protection
against
degradation,
enhanced
facilitation
of
endosomal
escape.
LNPs
encounter
numerous
challenges
targeted
vivo,
demanding
advanced
particle
engineering,
surface
functionalization
with
targeting
ligands,
profound
comprehension
the
biological
milieu
which
they
function.
This
review
explores
structural
physicochemical
characteristics
LNPs,
in-vivo
fate,
customization
therapeutics.
We
highlight
quality-by-design
(QbD)
approach
beyond
liver,
focusing
on
biodistribution,
immunogenicity,
toxicity.
In
addition,
we
explored
current
strategies
associated
ensuring
repeated-dose
efficacy,
safety,
tissue-specific
gene
delivery.
Furthermore,
provide
insights
into
clinical
applications
various
classes
diseases
finally
prospects
ACS Applied Bio Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 14, 2025
Conjugation
of
serum
albumin
protein
with
catechol-containing
dopamine
molecules
provides
an
alternative
method
for
the
preparation
nanoparticles
(NPs).
A
commonly
used
desolvation
utilizes
glutaraldehyde
as
a
cross-linking
agent.
Here,
catechol
mechanism
is
instead
providing
advantages
to
prevent
toxicity
and
undesirable
reaction
cargo
molecules.
Covalent
between
conjugated
bovine
(D-BSA)
proteins
was
obtained
in
presence
sodium
periodate
(NaIO4)
oxidizer.
As
result,
spherical
D-BSA
NPs
uniform
size
distribution
around
100
nm
diameter
negative
zeta
potential
−28
mV
were
prepared.
Optimal
conditions
reached
when
dopamine:IO4–
molar
ratio
2:1,
pH
7.4
medium,
acetone
desolvating
agent
used.
Furthermore,
display
antioxidant
properties,
have
rapid
biodegradability
trypsin,
high
doxorubicin
(DOX)
loading
(9.1%)
sustainable
drug
release.
DOX
loaded
also
caused
up
90%
breast
cancer
cell
(MCF-7)
death
within
24
h.
These
results
show
that
carrying
can
alternatively
be
prepared
via
covalently
cross-linked
groups
delivery
studies.
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
179, P. 117323 - 117323
Published: Aug. 28, 2024
Recently,
increased
attention
has
been
focused
on
the
regulatory
mechanism
and
potential
clinical
application
of
ferroptosis
in
cancer
cells,
especially
therapy-related
ferroptosis.
However,
treatment-related
prospects
strategies
for
future
treatment
still
require
further
clarification.
This
review
highlights
molecular
relationships
between
different
antitumour
drugs,
including
commonly
used
chemotherapy
radiation
therapy
vitamins,
also
proposes
treatments
that
involve
ferroptosis,
with
an
aim
to
develop
a
new
strategy
transformative
emerging
field
improve
therapy.
Langmuir,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 7, 2025
Therapeutic
proteins
play
a
crucial
role
in
modern
healthcare.
However,
the
rapid
clearance
of
circulation
system
poses
significant
threat
to
their
therapeutic
efficacy.
The
generation
anti-drug
antibodies
expedites
drug
clearance,
resulting
another
challenge
overcome
protein
delivery.
Several
methods
increase
half-lives
these
and
minimize
immunogenicity
have
been
developed.
This
Review
discusses
causes
body,
evaluates
FDA-approved
strategies
prolong
circulation,
highlights
recent
progress
field.
Additionally,
strengths
drawbacks
our
perspectives
for
advancing
delivery
are
provided.
International Journal of Nanomedicine,
Journal Year:
2025,
Volume and Issue:
Volume 20, P. 3493 - 3525
Published: March 1, 2025
Human
serum
albumin
(HSA)
has
emerged
as
a
promising
carrier
for
nanodrug
delivery,
offering
unique
structural
properties
that
can
be
engineered
to
overcome
key
challenges
in
cancer
treatment,
especially
resistance
chemotherapy.
This
review
focuses
on
the
cellular
uptake
of
albumin-based
nanoparticles
and
modifications
enhance
their
ability
bypass
mechanisms,
particularly
multidrug
type
1
(MDR1),
by
improving
targeting
cells.
In
our
approach,
we
integrate
chemical
albumin,
its
interactions
with
cells,
surface
delivery
systems
enable
those
related
MDR1,
precisely
target
receptors
cells
improve
treatment
efficacy.
We
discuss
while
well-established
such
gp60
gp18/30
are
crucial
transcytosis,
biology
remains
underexplored,
limiting
translational
potential.
Additionally,
explore
potential
emerging
targets,
cluster
differentiation
44
(CD44),
(CD36)
transferrin
receptor
TfR1,
well
advantages
using
dimeric
forms
(dHSA)
further
resistant
Drawing
from
clinical
examples,
including
success
albumin-bound
paclitaxel
(Abraxane)
new
formulations
like
Pazenir
Fyarro
(for
Sirolimus),
identify
gaps
current
knowledge
propose
strategies
optimize
systems.
conclusion,
nanoparticles,
when
tailored
appropriate
modifications,
have
By
enhancing
albumin's
efficiently
deliver
therapeutic
agents,
these
carriers
represent
approach
addressing
one
oncology's
most
persistent
challenges,
substantial
outcomes.
ACS Applied Bio Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 25, 2025
Chemodynamic
therapy
(CDT),
which
utilizes
transition
metal
ions
to
catalyze
Fenton-like
reactions
for
the
eradication
of
tumor
cells,
has
attracted
substantial
attention
in
field
nanocatalysis.
However,
therapeutic
efficacy
CDT
as
a
monotherapy
is
often
limited
by
an
insufficient
level
hydrogen
peroxide
(H2O2)
and
overexpressed
glutathione
(GSH)
within
cells.
Because
high
copper
content
tissues,
ionophore
was
strategically
employed
enhance
intracellular
accumulation
copper,
thereby
potentiating
effect.
Additionally,
bovine
serum
albumin
(BSA)
used
modify
ionophore,
naphthazarin
(Nap),
promote
its
targeting
cells
ensure
biosafety.
The
BSA-coated
Nap
nanoparticles,
could
recruit
Cu2+
situ,
were
further
deposited
onto
surface
manganese
carbonate
nanocube
(Nap-BM
NPs).
synergistic
action
accelerated
decomposition
H2O2
into
hydroxyl
radicals
(•OH)
consumed
GSH,
leading
cellular
mortality
via
mitochondrial
pathways.
With
low
cytotoxicity
good
biocompatibility
normal
developed
Nap-BM
NPs
significantly
enhanced
outcomes
leveraging
multiple
reaction
mechanisms
augment
CDT,
offering
promising
potential
clinical
applications
contributing
valuable
insights
field.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 8, 2025
Abstract
Nanodrug
delivery
systems
(NDDS)
have
demonstrated
broad
application
prospects
in
disease
treatment,
prevention,
and
diagnosis
due
to
several
advantages,
including
functionalization
capability,
high
drug‐loading
capacity,
drug
stability
protection,
the
enhanced
permeability
retention
(EPR)
effect.
However,
their
clinical
translation
still
faces
multiple
challenges,
rapid
clearance
by
reticuloendothelial
system
(RES),
poor
targeting
specificity,
insufficient
efficiency
crossing
biological
barriers.
To
address
these
limitations,
researchers
developed
carrier‐mediated
nanomedicine
hitchhiking
strategy
(BCM‐NHS),
which
leverages
circulating
cells,
proteins,
or
bacteria
as
natural
“mobile
carriers”
enhance
delivery.
This
approach
enables
nanocarriers
inherit
intrinsic
properties,
endowing
them
with
immune
evasion,
prolonged
circulation,
dynamic
targeting,
biocompatibility,
biodegradability,
naturally
optimized
interfaces.
Here,
a
systematic
overview
of
BCM‐NHS
is
provided.
First,
review
delves
into
methods
nanoparticles
(NPs)
binding
immobilization,
encompassing
both
surface‐attachment‐mediated
“backpack”
encapsulation‐based
“Trojan
horse”
strategy.
Second,
classification
carriers,
cell‐based
non‐cell‐based
elucidated.
Third,
physical
properties
release
mechanisms
nanomaterials
are
thoroughly
described.
Finally,
latest
applications
therapeutic
diagnostic
contexts
across
various
models
tumor,
ischemic
stroke,
pneumonia
highlighted.