Novel Insight into the Modulatory Effect of Traditional Chinese Medicine on Cerebral Ischemia-Reperfusion Injury by Targeting Gut Microbiota: A Review

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 185 - 200

Published: Jan. 1, 2025

Abstract: Cerebral ischemia-reperfusion injury (CIRI) is clinically characterized by high rates of morbidity, disability, mortality, and recurrence as well economic burden. The clinical manifestations CIRI are often accompanied gastrointestinal symptoms such intestinal bacterial dysbiosis bleeding. Gut microbiota plays an important role in the pathogenesis CIRI, its potential biological effects have received extensive attention. gut not only affects barrier function but also regulates immunity host homeostasis. Traditional Chinese medicine (TCM), a multi-component multi-targeted drug, has shown remarkable few adverse reactions prevention treatment CIRI. Notably, effect TCM on regulating maintaining homeostasis gradually become hot topic. This review summarizes functional development progression therapeutic improving dysbiosis, affecting metabolism, immunity. active ingredients used for relevant studies were saponins, triterpenoids, phenolics, alkaloids. In addition, to treat briefly discussed. established significance prospects TCM-based treatments provided necessary theoretical support further resources Keywords: cerebral injury, ischemic stroke, gut-brain axis, microbiota, traditional

Language: Английский

---

Novel insight into the role of A-kinase anchoring proteins (AKAPs) in ischemic stroke and therapeutic potentials

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 175, P. 116715 - 116715

Published: May 12, 2024

Ischemic stroke, a devastating disease associated with high mortality and disability worldwide, has emerged as an urgent public health issue. A-kinase anchoring proteins (AKAPs) are group of signal-organizing molecules that compartmentalize anchor wide range receptors effector have major role in stabilizing mitochondrial function promoting neurodevelopmental development the central nervous system (CNS). Growing evidence suggests dysregulation AKAPs expression activity is closely oxidative stress, ion disorder, dysfunction, blood-brain barrier (BBB) impairment ischemic stroke. However, underlying mechanisms remain inadequately understood. This review provides comprehensive overview composition structure protein (AKAP) family members, emphasizing their physiological functions CNS. We explored depth molecular cellular AKAP complexes pathological progression risk factors including hypertension, hyperglycemia, lipid metabolism disorders, atrial fibrillation. Herein, we highlight potential pharmacological target against stroke hope inspiring translational research innovative clinical approaches.

Language: Английский

---

Possible opportunities and challenges for traditional Chinese medicine research in 2035

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 21, 2024

The drug development process is poised for significant transformation due to the rapid advancement of modern biological and information technologies, such as artificial intelligence (AI). As these new technologies concepts infiltrate every stage development, efficiency success rate research are expected improve substantially. Traditional Chinese medicine (TCM), a time-honored therapeutic system encompassing herbal medicine, acupuncture, qigong, will also be profoundly impacted by advancements. Over next decade, encounter both opportunities challenges it integrates with concepts. By 2035, TCM anticipated merge through more contemporary open model, providing substantial support treating broader spectrum diseases.

Language: Английский

---

Extraction and purification of total flavonoids from Zanthoxylum planispinum Var. Dintanensis leaves and effect of altitude on total flavonoids content

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 27, 2025

Language: Английский

---

Exploring the Therapeutic Mechanism of Xinbao Pill in Brain Injury After Cardiopulmonary Resuscitation Based on Network Pharmacology, Metabolomics, and Experimental Verification

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(3)

Published: March 1, 2025

Post-cardiopulmonary resuscitation brain injury (PBI) is essentially the cerebral ischemia reperfusion (CIR) injury, which main cause of death and long-term disability in patients with cardiac arrest. So far, there no treatment for PBI; thus, it urgent to develop new drugs or therapies prevention after cardiopulmonary resuscitation. Although multiple constituent herbs active ingredients Xinbao Pill (XBP) have shown neuroprotective effects, whether XBP could play a therapeutic role on PBI still unknown. This study aimed illustrate effect probe underlying mechanisms. We first performed cell animal experiments validate protective neurological function. next identified potential differential metabolites via metabolomics analysis. further conducted comprehensive network pharmacology analysis including overlap gene analysis, protein-protein interaction network, gene-biological process-module function preliminarily investigate specific mechanism action (MOA) against PBI. Finally, PCR, MTT, ELISA assay, as well Western blotting were made our proposed molecular The vitro experiment showed that increase viability ameliorate morphological damage PC12 cells exposed oxygen-glucose deprivation reoxygenation (OGD/RO) conditions. vivo demonstrated improved Neurologic Deficit Score (NDS), lowered Neuron-Specific Enolase (NSE) level reversed typical neuropathological changes rats, indicating its Further 94 treatment, highly related CIR. Moreover, results revealed may be relevant mitochondrial quality control (MQC). Mechanistically, not only promote expressions marker proteins PGC1α, NRF1, TFAM, OPA1, MFN1 MFN2 biogenesis fusion but also inhibit those containing DRP1, MFF, FIS1, p62, PINK1, Parkin LC3 fission mitophagy. AMP-activated protein kinase (AMPK) inhibitor was crucial regulating MQC. Our determined might an anti-PBI formula, deciphered MOAs by approach combined experimental validation.

Language: Английский

---

Calycosin Inhibit PANoptosis and Alleviate Brain Damage: A Bioinformatics and Experimental Verification Approach

ACS Chemical Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

PANoptosis is a newly identified form of cell death that encompasses pyroptosis, apoptosis, and necroptosis. Numerous studies have highlighted the significance in brain ischemia-reperfusion (I/R) injury. Calycosin, natural product with diverse biological activities, has demonstrated significant reduction neuronal caused by ischemic injury modulating multiple pathways. In order to investigate potential mechanisms underlying neuroprotective role calycosin alleviating PANoptosis-induced damage stroke therapy, we used mouse hippocampal line HT22 stimulate ischemia vitro through Oxygen Glucose Deprivation/Reperfusion (OGD/R) established molecular docking assess binding affinity Calycosin key targets dynamics simulations (MDS) study stability ligand-protein complex. The results demonstrate could improve growth HT22, leading enhanced viability, reduced lactate dehydrogenase leakage, decreased apoptosis after OGD/R. It also regulated expression PANoptosis-related genes such as NLRP3, GSDMD, MLKL, RIPK1 increased Bcl-2/Bax ratio, effectively reducing cellular providing protection. Molecular MDS strong activity between targets. Furthermore, successfully passed drug similarity (DS) evaluation exhibited favorable absorption, distribution, metabolism, excretion, toxicity (ADMET) properties activity. conclusion, alleviate inhibiting PANoptosis, inflammation improving Thus, it serve therapy for stroke.

Language: Английский

---

Efficient In Vitro Propagation of Turpinia arguta and Quantitative Analysis of Its Ligustroflavone and Rhoifolin Content

Horticulturae, Journal Year: 2024, Volume and Issue: 10(6), P. 587 - 587

Published: June 4, 2024

Turpinia arguta is an excellent medicinal plant mainly used for the treatment of pharyngitis, tonsillitis, and tonsillar abscesses. However, efficient regeneration protocol using tissue cultures T. does not exist. Its main constituents are flavonoids, particularly ligustroflavone rhoifolin. Here, we aimed to establish a culture system first time annual stem segments with axillary buds harvested from field Jiangxi Academy Forestry as explants by dynamically determining accumulation effective functional components in plantlets. Orthogonal tests were conducted compare effects different explant disinfection times, media, exogenous hormone ratios on induction bud growth, successional proliferation, rooting segments. The best effect was achieved disinfecting 75% ethanol 50 s, followed 0.1% mercuric chloride (HgCl2) 6 min, optimal media proliferation Murashige Skoog (MS) + 0.2 mg/L 6-benzyladenine (6-BA), 0.03 naphthaleneacetic acid (NAA), ½ MS 2.5 indole-3-butyric 0.5 NAA, respectively. detection rhoifolin plantlets 0, 3, 5 months after transplanting showed significant increasing trend eventually exceeded content requirements 2020 Edition ofChinese Pharmacopoeia arguta. Our findings provide, time, arguta, thereby providing important information support germplasm preservation, innovation, application future.

Language: Английский

---

Nuclear factor erythroid 2-related factor-mediated signaling alleviates ferroptosis during cerebral ischemia-reperfusion injury

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 180, P. 117513 - 117513

Published: Sept. 27, 2024

Language: Английский

---

Metabolomic analysis of Agkistrodon haly venom poisoning mouse treatment by Jidesheng snake pill based on GC-MS

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 1, 2024

Introduction Snakebites are acute systemic toxic diseases caused by snake venom entering the body through wounds. Failure to use antivenom immediately and difficulty in obtaining antivenoms frequently responsible for worsening disease. Traditional Chinese medicine is commonly used supplement replace treating snakebites. The Jidesheng pill (JDS) a widely traditional that has achieved good clinical therapeutic effects; however, its mechanism remains unclear. Therefore, metabolomics techniques were employed explore pathophysiological mechanisms of JDS treatment Agkistrodon halys (Ah) venom-poisoned mice. Methods Ah group mouse model was established intramuscular injection into hind legs + using after affected area treated with venom. Hematoxylin eosin (HE) staining evaluate severity gastrocnemius injury. Quantitative polymerase chain reaction (qPCR) utilized detect mRNA expression vascular cell adhesion molecule-1 (VCAM-1), muscle-specific creatine kinase (CKM), thrombin antithrombin complex (TAT), tumor necrosis factor-alpha (TNF-α). Gas chromatography-mass spectrometry (GC-MS) performed multivariate statistical analysis provide new insights global metabolic profile Results HE revealed increased red necrosis, local hemorrhage, neutrophil infiltration than control group. Several compounds identified, including lipids, amino acids, peptides, organooxygen. Eighty differential metabolites screened between group, 24 groups. poisoning mice may involve aminoacyl-tRNA biosynthesis, various acid metabolism disorders, tricarboxylic circulation abnormal fatty metabolism. reduce symptoms affecting long-chain promoting nicotinamide-nicotinamide Conclusion Our results suggest huge prospects elucidating pathophysiology haly JDS.

Language: Английский

---

Luteolin alleviates airway remodeling in asthma by inhibiting the epithelial-mesenchymal transition via β-catenin regulation

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156090 - 156090

Published: Oct. 3, 2024

Language: Английский

---