Effect of fermentation on the constituents in the branches and leaves of Taxus media and non-small cell lung cancer DOI Creative Commons

Xing Guo,

Rui‐Sheng Wang, Zhenling Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Introduction Non-small cell lung cancer (NSCLC) is a prominent disease worldwide. Currently, commonly used methods, such as surgery and radiotherapy, have significant side effects. Traditional Chinese medicine (TCM) has become research hotspot because of its safe effective characteristics. The branches leaves Taxus media are abundant in antitumor active compounds, there been no conducted yet regarding anti–lung molecular mechanism. Objective aim this study to investigate the activity two samples before after fermentation T. , mechanism inhibitory effect on NSCLC. Methods chemical composition pre-fermentation (TM) post-fermentation qu (TMQ) were investigated using UHPLC-Q-Qrbitrap HRMS high-performance liquid chromatography (HPLC). anti-lung activities TM TMQ compared an A549-induced tumor mouse model. An enzyme-linked immunosorbent assay (ELISA), hematoxylin eosin (H&E) staining, immunohistochemistry, immunofluorescence determine action. Results results indicated that contained 83 consisting primarily flavonoids, organic acids, taxanes. Both taxanes flavonoids higher than TM. effectively inhibited growth non-small (NSCLC), inhibition rate was greater (57.24%) (49.62%). administration downregulated necrosis factor-α (TNF-α), interleukin-6 (IL-6), glutathione (GSH) level upregulated interferon-γ (IFN-γ), reactive oxygen species (ROS), malondialdehyde (MDA) levels serum mice. treatment also increased protein expression Bax, Caspase-3, Beclin-1 tissues. In contrast, bcl-2, PI3K, Ki67, ULK1, mTOR suppressed by TMQ. Protein analyses reemphasized superior over These cumulative findings demonstrated action closely related activation transcriptional misregulation pathway cholinergic synaptic, AMPK, PI3K/Akt/mTOR signaling pathways. Conclusion This ingredient contents effects . addition, herbal for NSCLC treatment.

Language: Английский

Identification of Natural Phytochemicals as AKT2 Inhibitors Using Molecular Docking and Dynamics Simulations as Potential Cancer Therapeutics DOI Creative Commons

Jibon Kumar Paul,

Mahir Azmal,

Md. Naimul Haque Shohan

et al.

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e41897 - e41897

Published: Jan. 1, 2025

The PI3K/AKT/mTOR pathway is central in regulating key cellular processes such as proliferation, survival, metabolism, and angiogenesis. Dysregulation of this pathway, particularly the AKT2 isoform, commonly observed cancers breast, ovarian, pancreatic cancers, leading to enhanced tumor progression, metastasis, therapeutic resistance. Therefore, targeting for inhibition a promising strategy cancer therapy. This study utilized molecular docking dynamics simulations identify natural phytochemicals that inhibit AKT2. Molecular results revealed millettone (CID 442810) exhibited highest binding affinity AKT2, with score -9.5 kcal/mol, followed by uzarigenin 92760), dihydrobiochanin A 439784), abyssinone I 442152) scores -9.0 -8.9 -8.7 kcal/mol respectively, outperforming control inhibitor, ipatasertib 24788740) -7.56 kcal/mol. indicated millettone, uzarigenin, demonstrated strong affinities stable interactions suggesting their potential agents involve hyperactivation. Notably, uzarigenin's superior stability, evidenced its lower root mean square deviation (RMSD), which measures structural solvent-accessible surface area (SASA), indicates compactness, highlights promise potent inhibitor Future vitro vivo studies will be crucial confirm efficacy these inhibitors reducing progression applications. Given also plays role neuronal survival plasticity, compounds may have applications neurodegenerative diseases Alzheimer's, warranting further investigation into dual relevance.

Language: Английский

Citations

0
Anticancer Activity of Phytochemicals from Nepalese Plants: A Review of Active Phytochemicals and Screening Methods DOI Creative Commons
Anup Luitel,

Lalit Mohan Pant,

Niru Thapa

et al.

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(3)

Published: March 1, 2025

Medicinal plants have long been sought after as a source of potentially valuable compounds to combat cancer. This review purposes that the phytochemicals found in Nepalese medicinal possess significant potential anticancer activity, which calls for methodical evaluation. The study explores methodologies employed screening activity Nepal. Full-text original research articles were searched on “Google Scholar” and “PubMed” using keywords “Anticancer” “Medicinal plants”. obtained plant species looked over National Herbarium Plant Laboratories (KATH) Database confirm their existence A list 71 showing was obtained. Active methods reported then analysed. contained 84 bioactive compounds, namely 7 alkaloids, 10 polyphenols, 1 saponins, 5 phytosterols, 29 terpenoids, 11 flavonoids, 3 polysaccharides, 18 other miscellaneous showed activities. Likewise, 77.78% studies used cell line-based vitro experiments while 6.94% animal-based vivo methods, remaining 15.28% combined both approaches. In particular, 54.51% papers multiple lines, single lines. These findings highlight trend significance these properties.

Language: Английский

Citations

0
Disafynol: A polyacetylene dimer from Centaurea schmidii enhancing breast cancer cell apoptosis via oxidative and ER stress pathways DOI
Zeinab Babaei, Mustafa Ghanadian, Mahmoud Aghaei

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Language: Английский

Citations

0
Emerging therapeutic approaches for cholangiocarcinoma: harnessing natural compounds and targeted therapies with proposed cellular mechanism DOI Creative Commons
Ansari Vikhar Danish Ahmad, Mohd Sayeed Shaikh, Subur W. Khan

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: March 31, 2025

Language: Английский

Citations

0
An updated review summarizing the anticancer potential of flavonoids via targeting NF-kB pathway DOI Creative Commons
Pratibha Pandey, Sorabh Lakhanpal, Danish Mahmood

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 6, 2025

Nuclear factor-κB (NF-κB) cell signaling pathway is essential for the progression and development of numerous human disorders, including cancer. NF-κB regulates a wide range physiological processes, such as survival, growth, migration. Deregulated NF-kB resulted in unregulated proliferation, viability, movement, invasion, thus promoting tumor development. Recent findings have increasingly shown that plant derived phytochemicals inhibit potential to be employed cancer therapeutics. Flavonoids are group polyphenolic natural compounds present various plants their fruits, vegetables, leaves. These medicinal properties owing antioxidant, anti-inflammatory, antiviral, antitumor characteristics. The main mechanism by which these flavonoids exhibit anticancer via potent antioxidative immunomodulatory actions. Current research reports demonstrated exhibited effects suppressing signaling. Based on facts, we comprehensively outlined role processes progression, drug resistance, angiogenesis metastasis. In addition these, also summarize specifically targeting types cancers.

Language: Английский

Citations

0
A Review of the Most Important Medicinal Plants for Reducing Dental Plaque in Traditional Iranian Medicine DOI

Melika Kakuyi Nezhad,

Aisan Ghaznavi

سپیده‌دم., Journal Year: 2024, Volume and Issue: 6(1), P. 69 - 75

Published: June 1, 2024

Language: Английский

Citations

0
Effect of fermentation on the constituents in the branches and leaves of Taxus media and non-small cell lung cancer DOI Creative Commons

Xing Guo,

Rui‐Sheng Wang, Zhenling Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Introduction Non-small cell lung cancer (NSCLC) is a prominent disease worldwide. Currently, commonly used methods, such as surgery and radiotherapy, have significant side effects. Traditional Chinese medicine (TCM) has become research hotspot because of its safe effective characteristics. The branches leaves Taxus media are abundant in antitumor active compounds, there been no conducted yet regarding anti–lung molecular mechanism. Objective aim this study to investigate the activity two samples before after fermentation T. , mechanism inhibitory effect on NSCLC. Methods chemical composition pre-fermentation (TM) post-fermentation qu (TMQ) were investigated using UHPLC-Q-Qrbitrap HRMS high-performance liquid chromatography (HPLC). anti-lung activities TM TMQ compared an A549-induced tumor mouse model. An enzyme-linked immunosorbent assay (ELISA), hematoxylin eosin (H&E) staining, immunohistochemistry, immunofluorescence determine action. Results results indicated that contained 83 consisting primarily flavonoids, organic acids, taxanes. Both taxanes flavonoids higher than TM. effectively inhibited growth non-small (NSCLC), inhibition rate was greater (57.24%) (49.62%). administration downregulated necrosis factor-α (TNF-α), interleukin-6 (IL-6), glutathione (GSH) level upregulated interferon-γ (IFN-γ), reactive oxygen species (ROS), malondialdehyde (MDA) levels serum mice. treatment also increased protein expression Bax, Caspase-3, Beclin-1 tissues. In contrast, bcl-2, PI3K, Ki67, ULK1, mTOR suppressed by TMQ. Protein analyses reemphasized superior over These cumulative findings demonstrated action closely related activation transcriptional misregulation pathway cholinergic synaptic, AMPK, PI3K/Akt/mTOR signaling pathways. Conclusion This ingredient contents effects . addition, herbal for NSCLC treatment.

Language: Английский

Citations

0