Developmental Dynamics,
Journal Year:
2021,
Volume and Issue:
250(10), P. 1420 - 1431
Published: March 24, 2021
Abstract
Background
Amphibians
possess
three
kinds
of
dermal
chromatophore:
melanophores,
iridophores,
and
xanthophores.
Knockout
Xenopus
tropicalis
that
lack
the
pigmentation
melanophores
iridophores
have
been
reported.
The
identification
causal
genes
for
xanthophore
or
differentiation
could
lead
to
creation
a
see‐through
frog
without
chromatophores.
causing
mutants
are
slc2a11b
slc2a15b
in
Japanese
medaka
(
Oryzias
latipes
).
Results
To
obtain
heritable
line
X
yellow
pigment,
we
generated
slc2a7
slc2a15a
knockout
animals
because
they
greatest
similarity
O
genes.
had
bluish
skin
there
were
no
visible
pigments
stereo
microscope
section
observations.
Furthermore,
pterinosomes,
which
characteristic
xanthophores,
observed
via
transmission
electron
microscopy
animals.
Conclusions
We
report
successful
generation
no‐yellow‐pigment
mutant
after
knock
out
gene.
This
finding
will
enable
with
Animal Microbiome,
Journal Year:
2021,
Volume and Issue:
3(1)
Published: Feb. 5, 2021
Abstract
Background
Historically
the
main
source
of
laboratory
Xenopus
laevis
was
environment.
The
increase
in
genetically
altered
animals
and
evolving
governmental
constraints
around
using
wild-caught
for
research
has
led
to
establishment
resource
centres
that
supply
reagents
worldwide,
such
as
European
Resource
Centre.
In
last
decade,
were
encouraged
keep
a
“low
microbial
load”
or
“clean”
state,
where
embryos
are
surface
sterilized
before
entering
housing
system;
instead
conventional,
“standard”
conditions
frogs
kept
without
prior
treatment.
Despite
having
been
captivity
almost
century,
surprisingly
little
is
known
about
holobiont
how
changing
microbiome
may
affect
resistance
disease.
This
study
examines
different
treatment
conditions,
husbandry
recirculating
housing,
affects
skin
tadpoles
female
adults.
particularly
important
when
considering
potential
poor
welfare
caused
by
change
method
move
from
smaller
colonies.
Results
We
found
strong
evidence
developmental
control
on
;
adults
had
extremely
similar
communities
independent
their
while
both
tadpole
environmental
less
resilient
showed
greater
diversity.
Conclusions
Our
findings
suggest
adult
controlled
selected
host.
indicates
stable
defined
independently
environment
which
it
housed,
suggesting
use
clean
poses
risk
transferring
laboratories.
will
have
implications
frog
health
applicable
throughout
world.
ILAR Journal,
Journal Year:
2021,
Volume and Issue:
62(1-2), P. 169 - 202
Published: Jan. 1, 2021
Abstract
Non-mammalian
vertebrates
including
birds,
fish,
and
amphibians
have
a
long
history
of
contributing
to
ground-breaking
scientific
discoveries.
Because
these
species
offer
several
experimental
advantages
over
higher
share
extensive
anatomic
genetic
homology
with
their
mammalian
counterparts,
they
remain
popular
animal
models
in
variety
fields
such
as
developmental
biology,
physiology,
toxicology,
drug
discovery,
immunology,
infectious
disease.
As
all
models,
familiarity
the
anatomy,
spontaneous
diseases
is
necessary
for
ensuring
welfare,
well
accurate
interpretation
reporting
study
findings.
Working
avian
aquatic
can
be
especially
challenging
this
respect
due
rich
diversity
array
unique
adaptations.
Here,
we
provide
an
overview
research-relevant
features,
non-infectious
conditions,
that
impact
research
colonies
birds
animals,
fish
Xenopus
species.
Journal of Cardiovascular Development and Disease,
Journal Year:
2023,
Volume and Issue:
10(2), P. 51 - 51
Published: Jan. 29, 2023
A
frog
is
a
classical
model
organism
used
to
uncover
processes
and
regulations
of
early
vertebrate
development,
including
heart
development.
Recently,
we
showed
that
also
represents
useful
study
rare
human
congenital
disease,
hypoplastic
left
syndrome.
In
this
review,
first
summarized
the
cellular
events
molecular
benefit
using
diseases.
Next,
described
challenges
in
elucidating
etiology
syndrome
discussed
how
may
contribute
our
understanding
bases
disease.
We
concluded
offers
its
unique
advantage
uncovering
mechanisms
syndrome;
however,
combining
multiple
organisms,
frogs,
needed
gain
comprehensive
Developmental Dynamics,
Journal Year:
2021,
Volume and Issue:
250(10), P. 1420 - 1431
Published: March 24, 2021
Abstract
Background
Amphibians
possess
three
kinds
of
dermal
chromatophore:
melanophores,
iridophores,
and
xanthophores.
Knockout
Xenopus
tropicalis
that
lack
the
pigmentation
melanophores
iridophores
have
been
reported.
The
identification
causal
genes
for
xanthophore
or
differentiation
could
lead
to
creation
a
see‐through
frog
without
chromatophores.
causing
mutants
are
slc2a11b
slc2a15b
in
Japanese
medaka
(
Oryzias
latipes
).
Results
To
obtain
heritable
line
X
yellow
pigment,
we
generated
slc2a7
slc2a15a
knockout
animals
because
they
greatest
similarity
O
genes.
had
bluish
skin
there
were
no
visible
pigments
stereo
microscope
section
observations.
Furthermore,
pterinosomes,
which
characteristic
xanthophores,
observed
via
transmission
electron
microscopy
animals.
Conclusions
We
report
successful
generation
no‐yellow‐pigment
mutant
after
knock
out
gene.
This
finding
will
enable
with
