Epigenetic signature of invasiveness: ChIP-seq profiling of H3K4me3 and H3K27me3 in an invasive insect DOI Open Access
Zhao Yan, Juntao Hu,

Jiajiao Wu

et al.

Authorea (Authorea), Journal Year: 2022, Volume and Issue: unknown

Published: July 4, 2022

While it has been suggested that histone modification can facilitate animal responses to rapidly changing environments, few studies have profiled whole-genome patterns in introduced species, leaving its role invasiveness unclear. Here, we screen genome-wide of two important modifications, trimethylated Histone H3 Lysine 4 (H3K4me3) and 27 (H3K27me3), adult thorax muscles a notorious invasive pest, the Oriental fruit fly Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), using Chromatin Immunoprecipitation with high-throughput sequencing (ChIP-seq). We identified active, repressed poised promoters, featured by occupancy H3K4me3, H3K27me3 bivalent modifications were respectively annotated unique genes key muscle development structure maintenance. In addition, found occupied entire body genes, average enrichment was almost constant. When comparing closely related indigenous Drosophila while highly conserved distribution function between more putative motifs modified may regulate insect flight capacity compared D. melanogaster. These findings provide first evidence signature an will be useful for future epigenetic regulation under global climate change.

Language: Английский

Insulin-Like Peptides and Cross-Talk With Other Factors in the Regulation of Insect Metabolism DOI Creative Commons
Szymon Chowański, Karolina Walkowiak‐Nowicka, Magdalena Joanna Winkiel

et al.

Frontiers in Physiology, Journal Year: 2021, Volume and Issue: 12

Published: June 29, 2021

The insulin-like peptide (ILP) and growth factor (IGF) signalling pathways play a crucial role in the regulation of metabolism, development, fecundity, stress resistance, lifespan. ILPs are encoded by multigene families that expressed nervous non-nervous organs, including midgut, salivary glands, fat body, tissue- stage-specific manner. Thus, more multidirectional complex control insect metabolism can occur. not only factors regulate metabolism. interact many cross-talk interactions different factors, for example, hormones (peptide nonpeptide), neurotransmitters factors. These observed at levels, three appear to be most prominent/significant: (1) coinfluence other on same target cells, (2) influence synthesis/secretion regulating (3) activity cells producing/secreting various For brain insulin-producing co-express sulfakinins (SKs), which cholecystokinin-like peptides, another key regulator express receptors tachykinin-related next involved It was also shown Drosophila melanogaster directly indirectly AKH. This review presents an overview regulatory peptides how these with players its regulation.

Language: Английский

Citations

67
Behavioral state-dependent modulation of insulin-producing cells in Drosophila DOI Creative Commons
Sander Liessem, Martina Held, Rituja S Bisen

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 33(3), P. 449 - 463.e5

Published: Dec. 28, 2022

Language: Английский

Citations

28
Genotype-by-Diet Interactions for Larval Performance and Body Composition Traits in the Black Soldier Fly, Hermetia illucens DOI Creative Commons
Christoph Sandrock,

Simon Leupi,

Jens Wohlfahrt

et al.

Insects, Journal Year: 2022, Volume and Issue: 13(5), P. 424 - 424

Published: April 30, 2022

Further advancing black soldier fly (BSF) farming for waste valorisation and more sustainable global protein supplies critically depends on targeted exploitation of genotype-phenotype associations in this insect, comparable to conventional livestock. This study used a fully crossed factorial design rearing larvae four genetically distinct BSF strains (FST: 0.11–0.35) three nutritionally different diets (poultry feed, food waste, poultry manure) investigate genotype-by-environment interactions. Phenotypic responses included larval growth dynamics over time, weight at harvest, mortality, biomass production with respective contents ash, fat, protein, including amino acid profiles, as well bioconversion nitrogen efficiency, reduction dry matter relevant fibre fractions, loss (emissions). Virtually all performance body composition traits were substantially influenced by diet but also characterised ample genetic variation and, most importantly, pronounced interaction effects between the two. Across evaluated phenotypes, variable diet-dependent rankings lack generally superior indicate involvement trade-offs traits, their relationships may even change signs. Conflicting resource allocation light overall fitness suggests anticipated breeding programs will require complex differential selection strategies account pinpointed trait maximisation versus multi-purpose resilience.

Language: Английский

Citations

21
Inter-organ Wingless/Ror/Akt signaling regulates nutrient-dependent hyperarborization of somatosensory neurons DOI Creative Commons
Yasutetsu Kanaoka, Koun Onodera, Kaori Watanabe

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 17, 2023

Nutrition in early life has profound effects on an organism, altering processes such as organogenesis. However, little is known about how specific nutrients affect neuronal development. Dendrites of class IV dendritic arborization neurons Drosophila larvae become more complex when the are reared a low-yeast diet compared to high-yeast diet. Our systematic search for key revealed that increase their terminal densities response combined deficiency vitamins, metal ions, and cholesterol. The these upregulates Wingless closely located tissue, body wall muscle. Muscle-derived activates Akt through receptor tyrosine kinase Ror, which promotes dendrite branching. In larval muscles, expression wingless regulated not only this nutrient-dependent manner, but also by JAK/STAT signaling pathway. Additionally, blunts light responsiveness avoidance behavior, may help optimize survival strategies under low-nutritional conditions. Together, our studies illustrate availability affects development inter-organ signaling.

Language: Английский

Citations

11
A short-term of starvation improved the antioxidant activity and quality of African catfish (Clarias gariepinus) DOI

Fanbin Zhan,

Qingqing Li,

Huiwen Feng

et al.

Fish Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 50(3), P. 911 - 925

Published: Feb. 1, 2024

Language: Английский

Citations

4
Silencing of the BtTPS genes by transgenic plant‐mediated RNAi to control Bemisia tabaciMED DOI
Gong Cheng,

Zezhong Yang,

Yuan Hu

et al.

Pest Management Science, Journal Year: 2021, Volume and Issue: 78(3), P. 1128 - 1137

Published: Nov. 19, 2021

Whitefly (Bemisia tabaci) is a typical pest that causes severe damage to hundreds of agricultural crops. The trehalose-6-phosphate synthase (TPS) genes, as the key genes in insect trehalose synthesis pathway, are important for growth and development. whitefly TPS may be main reason represent potential targets control whiteflies.In this study, we identified cloned three from B. tabaci MED found BtTPS1 BtTPS2 showed higher expression levels than BtTPS3 gene. Then, RNA interference (RNAi) resulted significant mortality influenced related involved energy metabolism chitin biosynthesis adults. Finally, transgenic tobacco plants effect on tabaci, knockdown or led retarded low hatchability nymphs, caused 90% decreased fecundity Additionally, with combinatorial RNAi better efficacy against whiteflies individual silencing.Our results suggest silencing BtTPS can compromise development whiteflies, offering not only new option but also secure environmentally friendly management strategy.

Language: Английский

Citations

25
Macronutrient-Based Predictive Modelling of Bioconversion Efficiency in Black Soldier Fly Larvae (Hermetia illucens) Through Artificial Substrates DOI Creative Commons
Laurens Broeckx, Lotte Frooninckx, Siebe Berrens

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(1), P. 77 - 77

Published: Jan. 14, 2025

This study explores the optimisation of rearing substrates for black soldier fly larvae (BSFL). First, ideal dry matter content was determined, comparing standard 30% (DM) with hydrated to their maximum water holding capacity (WHC). Substrates at maximal WHC yielded significantly higher larval survival rates (p = 0.0006). Consequently, approach adopted further experiments. Using these artificial substrates, fractional factorial designs based on central composite and Box–Behnken were employed assess impact macronutrient composition bioconversion efficiency. The results demonstrated significant main, interaction, quadratic effects Validation real-life varied protein content, including indigestible feather meal, affirmed predictive model’s accuracy after accounting source digestibility. research underscores importance optimal hydration in enhancing BSFL growth

Language: Английский

Citations

0
Insect Flight and Lipid Metabolism: Beyond the Classic Knowledge DOI
Leonardo L. Fruttero, Jimena Leyria, Lilián E. Canavoso

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Identification and Characterization of Troponin T Associated with Development, Metabolism and Reproduction in Tribolium castaneum DOI Open Access
Wenzhuo Li,

Yaning Sun,

Yuanye Liang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2786 - 2786

Published: March 19, 2025

As a tropomyosin-binding component, troponin T (TnT) is essential for the Ca2+ regulation of striated muscles’ contraction and locomotion activity, but its impacts on growth development insects have rarely been reported. In this study, TnT was identified functionally characterized in Tribolium castaneum by RNA interference (RNAi) transcriptome analysis. The T. contained 1152 bp open reading frame encoding 383 amino acids. It displayed highest expression late pupae highly expressed integument CNS. Both larval early pupal injection dsTnT led to 100% cumulative mortality before pupal–adult transition. Late RNAi caused 26.01 ± 4.29% mortality; survivors successfully became adults, 49.71 6.51% died 10 days with dried shriveled abdomen, poorly developed reproductive system no offspring. Additionally, sequencing results indicated that key ecdysteroid juvenile hormone biosynthesis genes (CYP314A1, aldehyde dehydrogenase family 3 member B1 farnesol dehydrogenase) were affected, as well several cuticle protein, nutrition metabolism immune-related genes, suggesting may play prominent roles development, reproduction affecting these pathways. This study could provide brand-new target gene strategy pest control.

Language: Английский

Citations

0
Shotgun metagenomics reveals the gut microbial diversity and functions in Vespa mandarinia (Hymenoptera: Vespidae) at multiple life stages DOI Creative Commons

Peng‐Kai Yin,

Huai Xiao,

Zhibin Yang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 11, 2024

Wasps play important roles as predators and pollinators in the ecosystem. The Jingpo minority residing Yunnan Province, China, has a traditional practice of using wine infused with mature wasps customary remedy for managing rheumatoid arthritis. larva wasp is also tasteful folk dish that created tremendous market. There paucity survival knowledge, which greatly restricted their potential applications food healthcare. Recent research highlighted importance gut microbiota insect growth. Nevertheless, there still lack understanding regarding composition, changes, functions Vespa mandarinia during development. In this research, were investigated across three growth stages metagenomic technology. result revealed are significant variations proportion main microbes metamorphosis . Tenericutes found to dominate larval stage, while Proteobacteria emerged dominant group post-pupation. Through comprehensive analysis metagenome, study functional differences at various stages. During plays central role promoting metabolism. Following pupation, exhibited diversified functions, likely due complex environments diverse sources encountered after metamorphosis. These included amino acid metabolism, compound degradation, defense mechanisms. This provides an extensive dataset on , contributing deeper influence Furthermore, uncovers unique microbial treasure within guts, advancing application fields medicine.

Language: Английский

Citations

3