bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown
Published: Dec. 21, 2022
Abstract Age is a fundamental aspect of biology that underlies the efficacy broad range functions. Identifying determinants for how quickly or slowly we age will contribute greatly to our understanding as modifier overall health, particularly advancement therapeutic interventions designed mitigate delay age-associated disorders. While much work has been devoted study genetic pharmacological extend lifespan, this approach does not necessarily recapitulate physiological profile naturally long-lived individuals. Diapause and diapause-like states constitute natural, inducible evolutionarily conserved examples lifespan plasticity are well-suited serve physiologically accurate models longevity. Here, leveraged metabolically critical signaling organ in Drosophila, fat body, examine diapause-associated transcription context chromatin accessibility regulation lifespan. Through combination ATAC-seq RNA-seq, observations suggest globally reorganized diapause may assume poised conformation facilitate rapid pro-development genes upon termination. We found particular significance GAF, NELF, RNA polymerase III context. Congruently, during appears favor many processes supporting maintenance cellular quiescence inhibition differentiation. Our data consistent with model wherein induces was additionally supported through fluorescent microscopy comparison public ChIP-seq developmentally juvenile files. This opens possibility longevity be partially determined lack mitogenic from quiescent niche, concurrent changes hormonal immunological profiles skew metabolism towards tissue maintenance.
Language: Английский