Hawkmoth pheromone transduction involves G protein-dependent phospholipase Cβ signaling DOI Open Access
Anna C. Schneider, Katrin Schröder, Yajun Chang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

1 Abstract Evolutionary pressures adapted insect chemosensation to the respective insect’s physiological needs and tasks in their ecological niches. Solitary nocturnal moths rely on acute olfactory sense find mates at night. Pheromones are detected with maximized sensitivity high temporal resolution through mechanisms that mostly unknown. While inverse topology of receptors heteromerization coreceptor Orco suggest ionotropic transduction via odorant-gated receptor-ion channel complexes, contradictory data propose amplifying G protein-coupled transduction. Here, we used vivo tip-recordings pheromone-sensitive sensilla male Manduca sexta hawkmoths specific times day (rest vs. activity). Since receptor neurons distinguish signal parameters three consecutive windows pheromone response (phasic; tonic; late, long-lasting), were analyzed separately. Disruption block phospholipase C decreased slowed phasic component during activity phase without affecting any other rest. A more targeted disruption α subunits by blocking αo or sustained activation αs using bacterial toxins affected response, while targeting αq α12/13 ineffective. Consistent these data, expression Cβ4 depended zeitgeber time, which indicates circadian clock-modulated metabotropic cascades maximize hawkmoth’s phase. Thus, discrepancies literature olfaction may be resolved considering timing distinct odor components. 2 Significance statement Insect chemosensory is typically thought ionotropic, but from different species suggests signaling occur, either alongside instead mechanisms. Nocturnal moths, known for extraordinarily sensitive pheromone-detecting neurons, likely use amplification. To overcome limitations previous vitro studies, conducted healthy times. Disrupting protein Cβ reduced altered kinetics, revealing strict control findings reconciled diverse evolutionary signals species, disparate parameters.

Language: Английский

Mutual coupling of neurons in the circadian master clock: what we can learn from fruit flies DOI Creative Commons
Charlotte Helfrich‐Förster, Nils Reinhard

Neurobiology of Sleep and Circadian Rhythms, Journal Year: 2025, Volume and Issue: 18, P. 100112 - 100112

Published: Jan. 18, 2025

Circadian master clocks in the brain consist of multiple neurons that are organized into populations with different morphology, physiology, and neuromessenger content presumably functions. In most animals, these distributed bilaterally, located close proximity to visual system, synchronized by eyes light-dark cycles environment. mammals cockroaches, each two consists a core region receives information from shell which output projections originate, whereas flies several other insects, lateral dorsal regions. all cases, morning evening clock seem exist, communication between them neurons, as well connection across hemispheres, is prerequisite for normal rhythmic function. Phenomena such rhythm splitting, internal desynchronization caused "decoupling" hemispheres or decoupling certain within one hemisphere. Since contain relatively few characterized at individual level, fly particularly suited study neurons. Here, we review organization bilateral brain, focus on synaptic paracrine connections comparison insects mammals.

Language: Английский

Citations

1
Chronobioethics: Symphony of biological clocks observed by 7-day/24-hour ambulatory blood pressure monitoring and cardiovascular health DOI Creative Commons

Kuniaki Otsuka,

Larry A. Beaty,

Madoka Sato

et al.

Biomedical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 100753 - 100753

Published: June 1, 2024

The high prevalence of desynchronized biological rhythms is becoming a primary public health concern. We assess complex and diverse inter-modulations among multi-frequency present in blood pressure (BP) heart rate (HR). Methods: performed 7-day/24-hour Ambulatory BP Monitoring 220 (133 women) residents (23 to 74 years) rural Japanese town Kochi Prefecture under everyday life conditions. A symphony clocks contributes the preservation synchronized circadian system. (1) Citizens with an average 12.02-h period had fewer vascular variability disorders than those shorter (11.37-h) or longer (12.88-h) periods (P<0.05), suggesting that circasemidian rhythm potentially important for human health. (2) An appropriate BP-HR coupling promoted healthier profiles phase-advanced BP: lower 7-day nighttime SBP (106.8 vs. 112.9 mmHg, P=0.0469), deeper nocturnal dip (20.5% 16.8%, P=0.0101), less frequent incidence masked non-dipping (0.53 0.86, P=0.0378), identifying night as time window. Adaptation irregular schedules occurs unconsciously at night, probably initiated from brain default mode network, coordination clock system, including reinforced about 12-hour clock, "a clock-guided core integration system".

Language: Английский

Citations

1
Hawkmoth pheromone transduction involves G protein-dependent phospholipase Cβ signaling DOI Open Access
Anna C. Schneider, Katrin Schröder, Yajun Chang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

1 Abstract Evolutionary pressures adapted insect chemosensation to the respective insect’s physiological needs and tasks in their ecological niches. Solitary nocturnal moths rely on acute olfactory sense find mates at night. Pheromones are detected with maximized sensitivity high temporal resolution through mechanisms that mostly unknown. While inverse topology of receptors heteromerization coreceptor Orco suggest ionotropic transduction via odorant-gated receptor-ion channel complexes, contradictory data propose amplifying G protein-coupled transduction. Here, we used vivo tip-recordings pheromone-sensitive sensilla male Manduca sexta hawkmoths specific times day (rest vs. activity). Since receptor neurons distinguish signal parameters three consecutive windows pheromone response (phasic; tonic; late, long-lasting), were analyzed separately. Disruption block phospholipase C decreased slowed phasic component during activity phase without affecting any other rest. A more targeted disruption α subunits by blocking αo or sustained activation αs using bacterial toxins affected response, while targeting αq α12/13 ineffective. Consistent these data, expression Cβ4 depended zeitgeber time, which indicates circadian clock-modulated metabotropic cascades maximize hawkmoth’s phase. Thus, discrepancies literature olfaction may be resolved considering timing distinct odor components. 2 Significance statement Insect chemosensory is typically thought ionotropic, but from different species suggests signaling occur, either alongside instead mechanisms. Nocturnal moths, known for extraordinarily sensitive pheromone-detecting neurons, likely use amplification. To overcome limitations previous vitro studies, conducted healthy times. Disrupting protein Cβ reduced altered kinetics, revealing strict control findings reconciled diverse evolutionary signals species, disparate parameters.

Language: Английский

Citations

0