Antioxidant taurine inhibits chondrocyte ferroptosis through upregulation of OGT/Gpx4 signaling in osteoarthritis induced by anterior cruciate ligament transection

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The aim of this study was to investigate the potential molecular mechanisms by which taurine protects against cartilage degeneration. anterior cruciate ligament transection (ACLT) surgery used construct an animal model osteoarthritis (OA). Metabolomics identify characteristic metabolites in osteoarthritic chondrocytes. Transcriptomics and metabolomics were explore small molecule metabolite inflammatory chondrocyte damage. Cell transfection inhibitors/agonists validate chondrocytes vitro. Finally, adeno-associated virus degeneration vivo. Metabolomic assays identified as a possible key metabolic progression OA. revealed that O-GlcNAc transferase (OGT)-dependent O-GlcNAcylation Gpx4-dependent ferroptosis may mediate protective effects on chondrocytes, further confirmed gain loss function Subsequently, experiments indicated existence direct binding site for Gpx4 OGT proteins, provides evidence presence modification protein. Finnaly, we demonstrated OGT-dependent be Antioxidant inhibits through upregulation OGT/Gpx4 signaling. Supplementation with taurine, safe nonessential amino acid, therapeutic strategy

Language: Английский

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Modulatory role of exogenous arachidonic acid in periodontitis with type 2 diabetes mellitus mice

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 19, 2025

Type 2 diabetes mellitus (T2DM) exhibits a bidirectional relationship with periodontitis, wherein each condition influences the progression of other. Arachidonic acid (AA) exerts an anti-diabetic effect, while showing protective effect by regulating inflammatory response independently its metabolites. However, impact on periodontitis T2DM remains poorly understood. The mouse model was established combining high-sugar and high-fat diet streptozotocin injection, followed silk ligation to induce periodontitis. Alterations in diabetes-associated symptoms were evaluated. Micro-computed tomography used measure bone-related parameters, including distance from cementoenamel junction alveolar bone crest, volume/total volume mineral density. Targeted metabolomics analysis conducted evaluate exogenous AA serum metabolite levels mice type diabetic 16S rRNA gene sequencing utilized analyze microbial diversity. activity osteoclasts, factors expression related osteoclasts investigated using TRAP staining real-time quantitative PCR. successfully established. Following two weeks treatment, reduction fasting blood glucose observed mice. Exogenous alleviated loss it had no substantial contents AA-targeted reduced Staphylococcus subgingival flora mice, but significant community structure or Furthermore, decreased number increased IL-10 mRNA gingival tissue. may alleviate reducing increasing periodontal tissues, rather than change metabolites composition diversity microorganisms plaque. These findings provide potential therapeutic approach for prevention treatment T2DM.

Language: Английский

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Editorial: O-GlcNAcylation and the immune system

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 20, 2024

Keywords: O-GlcNAcylation, immune system, inflammation, NF-KappaB, leukemia, immunometabolism, hypertension

Language: Английский

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O-GlcNAcylation in ovarian tumorigenesis and its therapeutic implications

Translational Oncology, Journal Year: 2024, Volume and Issue: 51, P. 102220 - 102220

Published: Nov. 30, 2024

Language: Английский

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