Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Oct. 7, 2021

An estimated 25 million tons of animal manure is produced globally every year, causing considerable impact to the environment. These impacts can be managed through use anaerobic digestion (AD) This process achieves waste degradation enzymatic activity, efficiency AD directly related microorganisms that produce these enzymes. Biomethane potential (BMP) assays remain standard theoretical framework pre-determine biogas yield and have been used determine feasibility substrates or their combination for production. However, an integrated approach combines substrate choice co-digestion would provide improvement current predictive models. PICRUSt (Phylogenetic Investigation Communities by Reconstruction Unobserved States) addresses limitations in this regard. In paper, biochemical functions horse, cow, pig manures are predicted. A total 135 predicted KEGG Orthologies (KOs) showed amino acids, carbohydrate, energy, lipid, xenobiotic metabolisms all samples. Linear discriminant analysis (LDA) combined with effect size measurements (LEfSe), fructose, mannose, acid nucleotide sugar, phosphotransferase (PST) as well starch sucrose were significantly higher horse 36 KOs acidogenesis and/or acetogenesis stages. Extended bar plots 11 significant predictions observed horse-cow, while 5 horse-pig cow-pig manures. Based on predictions, enhanced strategies takes into account metabolic contributions The results supported BMP calculations samples study. Biogas yields improved if employed routine before co-digesting different substrates.

Language: Английский

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Tweaking genome-editing approaches for virus interference in crop plants

Plant Physiology and Biochemistry, Journal Year: 2019, Volume and Issue: 147, P. 242 - 250

Published: Dec. 19, 2019

Language: Английский

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Advances and Challenges in Bacterial Spot Resistance Breeding in Tomato (Solanum lycopersicum L.)

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(5), P. 1734 - 1734

Published: March 3, 2020

Bacterial spot is a serious disease of tomato caused by at least four species Xanthomonas. These include X. euvesicatoria (race T1), vesicatoria T2), perforans (races T3 and T4), gardneri, with the distinct geographical distribution each group. Currently, gardneri are two major bacterial pathogens in North America, T4) dominating east-coast while Midwest. The causes up to 66% yield loss. Management this challenging due lack useful chemical control measures commercial resistant cultivars. Although genes for resistance (R) quantitative have been identified, breeding has impeded multiple factors including emergence new races pathogen that overcome resistance, multigenic linkage drag, non-additive components low correlation between seedling assays field resistance. Transgenic Bs2 EFR was effective against However, it not commercialized because public concerns complex regulatory processes. genomics-assisted breeding, effectors-based genomics genome editing technology could be novel approaches achieve durable tomato. main goal paper understand current status its diversity, challenges management, sources, genetics future prospectives approaches.

Language: Английский

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Genomics Enabled Breeding Strategies for Major Biotic Stresses in Pea (Pisum sativum L.)

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: May 18, 2022

Pea ( Pisum sativum L.) is one of the most important and productive cool season pulse crops grown throughout world. Biotic stresses are crucial constraints in harnessing potential productivity pea warrant dedicated research developmental efforts to utilize omics resources advanced breeding techniques assist rapid timely development high-yielding multiple stress-tolerant–resistant varieties. Recently, researcher’s community has made notable achievements conventional molecular accelerate its genetic gain. Several quantitative trait loci (QTLs) or markers associated with genes controlling resistance for fusarium wilt, root rot, powdery mildew, ascochyta blight, rust, common broomrape, enation, seed borne mosaic virus available marker-assisted breeding. The genomic tools such as availability comprehensive maps linked reliable DNA hold great promise toward introgression from different sources speed up gain pea. This review provides a brief account recent past regarding resources’ development, inheritance various biotic stress responses pathogenesis disease causing organisms, genes/QTLs mapping, transcriptomic proteomic advances. Moreover, emerging new approaches transgenics, genome editing, selection, epigenetic breeding, transform Overall, judicious amalgamation modern omics-enabled strategies will augment could hasten stress-resistant cultivars sustain production under changing climate. present encompasses at platform accomplishment so far improvement respect major way forward enhance through technologies.

Language: Английский

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Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat

Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14

Published: April 18, 2023

Genomics and genome editing promise enormous opportunities for crop improvement elementary research. Precise modification in the specific targeted location of a has profited over unplanned insertional events which are generally accomplished employing unadventurous means genetic modifications. The advent new procedures viz; zinc finger nucleases (ZFNs), homing endonucleases, transcription activator like effector (TALENs), Base Editors (BEs), Primer (PEs) enable molecular scientists to modulate gene expressions or create novel genes with high precision efficiency. However, all these techniques exorbitant tedious since their prerequisites difficult processes that necessitate protein engineering. Contrary first generation modifying methods, CRISPR/Cas9 is simple construct, clones can hypothetically target several locations different guide RNAs. Following model application help module, various customized Cas9 cassettes have been cast off advance mark discrimination diminish random cuts. present study discusses progression apparatuses, applications chickpea development, scientific limitations, future perspectives biofortifying cytokinin dehydrogenase, nitrate reductase, superoxide dismutase induce drought resistance, heat tolerance higher yield encounter global climate change, hunger nutritional threats.

Language: Английский

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