High-throughput sequencing in plant disease management: a comprehensive review of benefits, challenges, and future perspectives

Phytopathology Research, Journal Year: 2023, Volume and Issue: 5(1)

Published: Sept. 12, 2023

Abstract High-throughput sequencing (HTS) has instigated a paradigm shift in plant pathology, showcasing its transformative role the management of diseases. As powerful tool, HTS aids identifying pathogens and enhances disease strategies by detecting novel emerging pathogens, tracking outbreaks, contributing to developing disease-resistant cultivars. Despite these benefits, implementation faces obstacles due complexity data interpretation economic factors that affect widespread adoption. This comprehensive review summarizes strengths, limitations, opportunities associated with using managing The article also delves into prospects HTS, incorporating technological advancements, synergy complementary methodologies, capacity-building initiatives, development best practices guidelines. By acknowledging addressing while harnessing full capabilities we advocate for refined approach is critical ensuring global food security, especially context growing population climate change.

Language: Английский

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Breeding for Biotic Stress Resistance in Pea

Agriculture, Journal Year: 2023, Volume and Issue: 13(9), P. 1825 - 1825

Published: Sept. 18, 2023

Pea (Pisum sativum) stands out as one of the most significant and productive cool-season pulse crops cultivated worldwide. Dealing with biotic stresses remains a critical challenge in fully harnessing pea’s potential productivity. As such, dedicated research developmental efforts are necessary to make use omic resources advanced breeding techniques. These approaches crucial facilitating rapid timely development high-yielding varieties that can tolerate resist multiple stresses. The availability genomic tools, such comprehensive genetic maps reliable DNA markers, holds immense promise for integrating resistance genes from diverse sources. This integration helps accelerate gains pea crops. review provides an overview recent accomplishments resource peas. It also covers inheritance controlling various stress responses, control pathogenesis disease-causing organisms, mapping genes/QTLs, well transcriptomic proteomic advancements. By combining conventional modern omics-enabled strategies, be significantly enhanced.

Language: Английский

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Crop Cultivation and Hybrid Seed Production Strategies in Rapeseed-Mustard

Published: Jan. 1, 2025

Language: Английский

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Transcriptomic analysis of the symbiotic responsivity trait in pea (Pisum sativum L.)

Vavilov Journal of Genetics and Breeding, Journal Year: 2025, Volume and Issue: 29(2), P. 248 - 258

Published: April 10, 2025

Pea ( Pisum sativum L .) is an important crop culture and a model object for studying the molecular genetic bases of nitrogen-fixing symbiosis arbuscular mycorrhiza (AM). genotypes with high low responsivity to inoculation nodule bacteria (rhizobia) AM fungi have been described: ‘responsive’ demonstrate increase in seed weight under inoculation, while ‘non-responsive’ ones do not show such reaction. In order get insight into mechanisms underlying symbiotic responsivity, transcriptomic analysis whole root systems pea plants genotype k-8274 (cv. Vendevil, France) k-3358 (unnamed cultivar, Saratov region, Russia) grown soil without (control) inoculated either rhizobia (single inoculation) or together (double was performed. It shown that genotype, indeed, demonstrated pronounced response single double contrast genotype. k-8274, led specific up-regulation genes related catabolism polyamines, lipid metabolism, jasmonic acid salicylic signaling. Under specifically up-regulated were infection, down-regulated nodulation. This fact matches phenotype plants: number nodules lower as compared control. Thus, strict control over may be one pea. Finally, comparison expression profiles roots also allowed us identify signatures characteristic symbiotically responsive Further work will focused on validation these markers trait

Language: Английский

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Deciphering growth abilities of fusarium oxysporum f. sp. pisi under variable temperature, pH and nitrogen

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 3, 2023

Fusarium wilt caused by oxysporum f. sp. pisi (Fop) is an important disease and major obstacle to pea production, causing huge losses growers. The focus of this study was on isolation followed morphological, molecular characterization analyzing the growth casual agent under variable temperature, pH Nitrogen levels. morphological features radial growth, sporulation, pigmentation mycelial were examined variability all isolates presented. Molecular fungus ITS rDNA sequencing revealed that 13 belong species. Six tested for nitrogen dosage optimization studies. Seven different temperatures, viz., 21, 23, 25, 27, 29, 31, 33°C values, having 3, 4, 5, 6, 7, 8, 9 pH, as well levels 0 g, 3 5 7 11 g against six isolates, respectively. results showed exhibited highest at a temperature 25°C optimal range 23-27°C. All pH5. Change in doses base ended formation thick, dense, fluffy mycelium agent. used combination studies with seven dosages. density plots variations changes levels, which can lead mutations or genetic pathogens could potentially introduce new threats cultivation.

Language: Английский

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Temporal transcriptomics provides insights into host‒pathogen interactions: a case study of Didymella pinodella and disease-resistant and disease-susceptible pea varieties

Crop Health, Journal Year: 2023, Volume and Issue: 1(1)

Published: Aug. 10, 2023

Abstract Ascochyta blight is a fungal disease affecting peas, causing significant damage to the plant and reducing crop yield. Host‒pathogen interactions can inform prevention control strategies but remain poorly understood. Here, we generate near-chromosome-level assembly for Didymella pinodella HNA18, pathogenic fungus that causes pea ascochyta blight. Comparative genomic analysis of D. HNA18 seven publicly available genomes revealed genome encodes most conserved biosynthetic gene clusters (BGCs) similar number carbohydrate-activating enzyme (CAZyme) genes compared other species. Furthermore, by sequencing analyzing transcriptomic data disease-susceptible disease-resistant varieties during infection process, found mobilized set attack varieties, timing intensity these were different. For in response fungus, types defense genes, while used higher relative entire process. This study not only provides multiomic resources against its also deciphers mode interaction between defense.

Language: Английский

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Advances in pea breeding and genomics: From traditional techniques to modern approaches

Vegetable Science, Journal Year: 2023, Volume and Issue: 50(01), P. 1 - 10

Published: Aug. 12, 2023

Peas, a highly valued annual legume vegetable with rich history of domestication, are grown globally as valuable exportoriented cash crop. Despite an increase in cultivation area and production, there has been only slight improvement green pea productivity, from 7.7 to 7.8 t/ha, over the last two decades. The primary focus for genetic peas is developing resistance various biotic stressors, including diseases such powdery mildew, downy rust, wilt, viral infections, bacterial blight, well pests like leaf miners, aphids, pod borers, stem flies. Traditional reeding approaches have played significant role peas, resulting development veral cultivars segments; however, advanced breeding techniques marker-assisted selection, genomic genome editing hold great promise enhancing by facilitating identification selection desirable traits, abiotic improved yield, increased nutrient content, through introduction precise modifications. By targeting specific regions associated desired these can efficiency precision programs, ultimately leading more resilient productive varieties.

Language: Английский

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Genome and Transcriptome Analysis of Ascochyta pisi Provides Insights into the Pathogenesis of Ascochyta Blight of Pea

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(1)

Published: Jan. 16, 2023

Ascochyta blight caused by pisi is a major constraint to pea (Pisum sativum L.) production worldwide. Deciphering the pathogenic mechanism of

Language: Английский

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Understanding the root of the problem for tackling pea root rot disease

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 24, 2024

Pea (

Language: Английский

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Plant breeding is needed to meet agroecological requirements: Legume crops as a case study

Outlook on Agriculture, Journal Year: 2023, Volume and Issue: 52(3), P. 294 - 302

Published: Sept. 1, 2023

Legume-based cropping systems are regaining interest due to the environmental services and variety of food feed uses they provide, this having special in agroecological systems. There many legume crops that can be adopted but focused breeding is needed better meet specific requirements each system, especially situation changing climate often stressful environments. Standard methods remain valid, from classical selection, genomic assisted-breeding, profiting modern biotechnological approaches which rapidly developing for most crops. Rather than focusing on tools, emphasis targets, might different those high input agriculture such as improved response symbiosis, nutrient water use efficiency tolerance biotic abiotic stresses becoming priorities. Breeding these traits requires infusion genetic diversity landraces or wild relatives by pre-breeding. Prospects constraints discussed.

Language: Английский

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