Interactions Between Carbon Metabolism and Photosynthetic Electron Transport in a Chlamydomonas reinhardtii Mutant Without CO2 Fixation by RuBisCO DOI Creative Commons
Maureen Saint‐Sorny, Pawel Brzezowski, Stéphanie Arrivault

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: April 28, 2022

A Chlamydomonas reinhardtii RuBisCO-less mutant, ΔrbcL, was used to study carbohydrate metabolism without fixation of atmospheric carbon. The regulatory mechanism(s) that control linear electron flow, known as "photosynthetic control," are amplified in ΔrbcL at the onset illumination. With aim understand metabolites this response, we have correlated kinetics primary carbon chlorophyll fluorescence induction curves. We identify absence acetate generates adenosine triphosphate (ATP) via photosynthetic transfer reactions. Also, Calvin Benson Bassham (CBB) cycle responsive light. Indeed, ribulose 1,5-bisphosphate (RuBP), last intermediate before carboxylation by Ribulose-1,5-bisphosphate carboxylase-oxygenase, accumulates significantly with time, and CBB intermediates for RuBP regeneration, dihydroxyacetone phosphate (DHAP), pentose phosphates ribose-5-phosphate (R5P) rapidly accumulated first seconds illumination, then consumed, showing although is blocked, these enzymes still transiently active. In opposition, presence acetate, consumption strongly diminished, suggesting link between light almost lost. Phosphorylated hexoses starch accumulate significantly. show uptake results heterotrophic dominating phototrophic metabolism, glyoxylate tricarboxylic acid (TCA) being most highly represented metabolites, specifically succinate malate. These findings allow us hypothesize which metabolic pathways relevant upregulation processes like cyclic flow implicated mechanisms.

Language: Английский

Advances in microalgae-based carbon sequestration: Current status and future perspectives DOI

Udaypal Udaypal,

Rahul Kumar Goswami, Sanjeet Mehariya

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 249, P. 118397 - 118397

Published: Feb. 2, 2024

Language: Английский

Citations

27
Calvin–Benson cycle regulation is getting complex DOI
Libero Gurrieri, Simona Fermani, Mirko Zaffagnini

et al.

Trends in Plant Science, Journal Year: 2021, Volume and Issue: 26(9), P. 898 - 912

Published: April 21, 2021

Language: Английский

Citations

79
Real-time monitoring of subcellular H2O2 distribution in Chlamydomonas reinhardtii DOI Creative Commons
Justus Niemeyer, David Scheuring, Julian Oestreicher

et al.

The Plant Cell, Journal Year: 2021, Volume and Issue: 33(9), P. 2935 - 2949

Published: June 30, 2021

Hydrogen peroxide (H2O2) is recognized as an important signaling molecule in plants. We sought to establish a genetically encoded, fluorescent H2O2 sensor that allows monitoring all major subcompartments of Chlamydomonas cell. To this end, we used the Modular Cloning toolbox target hypersensitive reduction–oxidation sensitive green protein2-Tsa2ΔCR cytosol, nucleus, mitochondrial matrix, chloroplast stroma, thylakoid lumen, and endoplasmic reticulum (ER). The was functional compartments, except for ER where it fully oxidized. Employing our novel sensors, show produced by photosynthetic linear electron transport (PET) stroma leaks into cytosol but only reaches other subcellular compartments if under nonphysiological conditions. Furthermore, heat-stressed cells, cytosolic levels closely mirror temperature up- downshifts are independent from PET. Heat stress led similar nucleus and, more mildly, mitochondria not chloroplast. Our results thus suggest establishment steep intracellular gradients normal physiological conditions with limited diffusion compartments. anticipate these sensors will greatly facilitate future investigations biology plant cells.

Language: Английский

Citations

67
Carbon capture, storage, and usage with microalgae: a review DOI
Shweta Tripathi,

Sonia Choudhary,

Alok Meena

et al.

Environmental Chemistry Letters, Journal Year: 2023, Volume and Issue: 21(4), P. 2085 - 2128

Published: May 24, 2023

Language: Английский

Citations

39
Overexpression of Sedoheptulose-1,7-Bisphosphatase Enhances Photosynthesis in Chlamydomonas reinhardtii and Has No Effect on the Abundance of Other Calvin-Benson Cycle Enzymes DOI Creative Commons

Alexander Hammel,

Frederik Sommer, David Zimmer

et al.

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: June 23, 2020

The productivity of plants and microalgae needs to be increased feed the growing world population promote development a low-carbon economy. This goal can achieved by improving photosynthesis via genetic engineering. In this study, we have employed Modular Cloning strategy overexpress Calvin-Benson cycle (CBC) enzyme sedoheptulose-1,7 bisphosphatase (SBP1) up 3-fold in unicellular green alga Chlamydomonas reinhardtii. protein derived from nuclear transgene represented ~0.3% total cell protein. Photosynthetic rate growth were significantly SBP1-overexpressing lines under high-light elevated CO2 conditions. Absolute quantification abundance all other CBC enzymes QconCAT approach revealed no consistent differences between recipient strain. analysis also that eleven represent 11.9% Chlamydomonas. Here range concentrations turned out much larger than estimated earlier, with 128-fold difference most abundant (rbcL) least (triose phosphate isomerase). Accordingly, intermediates are often but not always higher binding site for which they act as substrates. highest substrate ratios might good candidates overexpression subsequent engineering steps.

Language: Английский

Citations

57
Microalgal-Based Bioenergy: Strategies, Prospects, and Sustainability DOI

Sonia Choudhary,

Shweta Tripathi, Krishna Mohan Poluri

et al.

Energy & Fuels, Journal Year: 2022, Volume and Issue: 36(24), P. 14584 - 14612

Published: Dec. 2, 2022

The fuel crisis with the slumping reserves of fossil fuels and exponential increase in demand energy necessitate a paradigm shift to more sustainable eco-friendly system. Microalgal biofuel has been recognized as one most prominent versatile alternative renewable sources because it can be converted into wide array biofuels, such biodiesel, bioethanol, bioelectricity biogases syngas, methane, hydrogen, hythane etc., lower carbon emission profile. To attain economic viability algal fuels, collective biorefinery approach addressing both operational technical limitations is quintessential. current review critically analyzes categorizes aspects microalgae through various direct indirect approaches perspective circular economy. These include complicated thermochemical biochemical processes integrating recovery range value-added products apart from thus defining cascading for multi-product-based model. A glimpse recent technological advancements comprising hybrid cultivation systems, prospects co-culturing, role automated/modeling industrial-scale production "algal-based bioenergy" also discussed detail. Further, scrutinized highlighted developments bottlenecks genetic molecular strain improvement bioenergy production. Furthermore, study correlated socioeconomic environmental impacts producing this green by evaluating pitfalls obtain holistic picture on bio-based

Language: Английский

Citations

38
Challenges and prospects for sustainable microalga-based oil: A comprehensive review, with a focus on metabolic and genetic engineering DOI
Saeed Ranjbar, F. Xavier Malcata

Fuel, Journal Year: 2022, Volume and Issue: 324, P. 124567 - 124567

Published: May 18, 2022

Language: Английский

Citations

31
Microalgal bioengineering: a futuristic tool for carbon capture DOI Creative Commons
Adamu Yunusa Ugya, Yangyang Sheng, Hui Chen

et al.

Results in Engineering, Journal Year: 2024, Volume and Issue: unknown, P. 102990 - 102990

Published: Sept. 1, 2024

Citations

6
Proteomics and constraint-based modelling reveal enzyme kinetic properties of Chlamydomonas reinhardtii on a genome scale DOI Creative Commons
Marius Arend, David Zimmer,

Rudan Xu

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 8, 2023

Metabolic engineering of microalgae offers a promising solution for sustainable biofuel production, and rational design strategies can be improved by employing metabolic models that integrate enzyme turnover numbers. However, the coverage numbers Chlamydomonas reinhardtii, model eukaryotic microalga accessible to engineering, is 17-fold smaller compared heterotrophic cell factory Saccharomyces cerevisiae. Here we generate quantitative protein abundance data covering 2337 3708 proteins in various growth conditions estimate vivo maximum apparent Using constrained-based modeling provide proxies 568 reactions, representing 10-fold increase over vitro Chlamydomonas. Integration estimates instead values accuracy usage predictions. Our results help extending knowledge on uncharacterized enzymes improve biotechnological applications

Language: Английский

Citations

15
Multifaceted applications of microalgal biomass valorization to enriched biorenewables, a review of futuristic biorefinery paradigm DOI Open Access
Mohammed Rehmanji, Rabinder Singh, Asha A. Nesamma

et al.

Bioresource Technology Reports, Journal Year: 2022, Volume and Issue: 17, P. 100972 - 100972

Published: Feb. 1, 2022

Language: Английский

Citations

19