Plant metabolomics: applications and challenges in the era of multi-omics big data

aBIOTECH, Journal Year: 2025, Volume and Issue: 6(1), P. 116 - 132

Published: Jan. 23, 2025

Abstract Plant metabolites are crucial for the growth, development, environmental adaptation, and nutritional quality of plants. metabolomics, a key branch systems biology, involves comprehensive analysis interpretation composition, variation, functions these metabolites. Advances in technology have transformed plant metabolomics into sophisticated process involving sample collection, metabolite extraction, high-throughput analysis, data processing, multidimensional statistical analysis. In today’s era big data, field is witnessing an explosion acquisition, offering insight complexity dynamics metabolism. Moreover, multiple omics strategies can be integrated to reveal interactions regulatory networks across different molecular levels, deepening our understanding biological processes. this review, we highlight recent advances challenges emphasizing roles technique improving crop varieties, enhancing value, increasing stress resistance. We also explore scientific foundations its applications medicine, ecological conservation.

Language: Английский

---

Diversity and divergence: evolution of secondary metabolism in the tropical tree genus Inga

New Phytologist, Journal Year: 2022, Volume and Issue: 237(2), P. 631 - 642

Published: Oct. 20, 2022

Summary Plants are widely recognized as chemical factories, with each species producing dozens to hundreds of unique secondary metabolites. These compounds shape the interactions between plants and their natural enemies. We explore evolutionary patterns processes by which generate diversity, from evolving novel profiles. characterized profile one‐third tropical rainforest trees in genus Inga ( c. 100, Fabaceae) using ultraperformance liquid chromatography‐mass spectrometry‐based metabolomics applied phylogenetic comparative methods understand mode evolution. show: contain structurally unrelated high levels phytochemical diversity; closely related have divergent profiles, individual compounds, compound classes, profiles showing little‐to‐no signal; at time scale, a species' shows signature adaptation. At ecological sympatric were most divergent, implying it is also advantageous maintain community members; finally, we integrate these model for how diversity evolves. Taken together, results show that divergence fundamental ecology evolution plants.

Language: Английский

---

Intraspecific chemodiversity provides plant individual- and neighbourhood-mediated associational resistance towards aphids

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 4, 2023

Some plant species express an extraordinarily high intraspecific diversity in phytochemicals (= chemodiversity). As discussed for biodiversity, higher chemodiversity may provide better protection against environmental stress, including herbivory. However, little is known about whether the resistance of a individual towards herbivores mostly governed by its own or associational provided conspecific neighbours. To investigate role plant-aphid interactions, we used Asteraceae Tanacetum vulgare, whose individuals differ pronouncedly composition leaf terpenoids, forming distinct chemotypes. Plants were set up field consisting plots containing five either same different Presence winged aphids, indicating attraction, and abundance unwinged fitness, counted weekly on each plant. During peak samples taken from all plants re-analyses terpenoid quantification chemodiversity, calculated (Shannon index, Hsind, also considered as α-chemodiversity) plot level (Hsplot, = β-chemodiversity). Aphid attraction was neither influenced chemotype nor plot-type. The real-time odour environment be very complex this setting, impeding clear preferences. In contrast, affected both On average, more Uroleucon tanaceti aphids found two chemotypes growing homogenous compared to heterogenous plots, supporting hypothesis. For Macrosiphoniella tanacetaria probability presence differed between plot-types one chemotype. Terpenoid expressed gradient revealed negative Hsplot effects U. tanaceti, but positive correlation Hsind with M. abundance. Aphids fuscoviride not any chemodiversity. conclusion, study shows that only neighbours can influence certain plant-herbivore interactions. These are highly specific regard aphid their morphs (winged vs. unwinged). Furthermore, our results highlight importance analysing at levels.

Language: Английский

---

Variability in Plant–Herbivore Interactions

Annual Review of Ecology Evolution and Systematics, Journal Year: 2023, Volume and Issue: 54(1), P. 451 - 474

Published: Aug. 22, 2023

Plants and herbivores are remarkably variable in space time, variability has been considered a defining feature of their interactions. Empirical research, however, traditionally focused on understanding differences means overlooked the theoretically significant ecological evolutionary roles itself. We review literature with goal showing how variability-explicit research expands our perspective plant–herbivore ecology evolution. first clarify terminology for describing variation then patterns, causes, consequences herbivory across scales space, biological organization. consider incorporating improves existing hypotheses leads to new ones. conclude by suggesting future work that reports full distributions, integrates effects scales, describes nonlinearities, considers stochastic deterministic combine determine distributions.

Language: Английский

---

Metabolomic Analysis Reveals Nutritional Diversity among Three Staple Crops and Three Fruits

Foods, Journal Year: 2022, Volume and Issue: 11(4), P. 550 - 550

Published: Feb. 15, 2022

More than 2 billion people worldwide are under threat of nutritional deficiency. Thus, an in-depth comprehension the composition staple crops and popular fruits is essential for health. Herein, we performed LC-MS-based non-targeted targeted metabolome analyses with (including wheat, rice, corn) grape, banana, mango). We detected a total 2631 compounds by using strategy identified more 260 nutrients. Our work discovered species-dependent accumulation common present nutrients in fruits. Although rice wheat lack vitamins amino acids, sweet corn was rich most acids vitamins. Among three fruits, mango had grape banana. Grape banana provided sufficient 5-methyltetrahydrofolate vitamin B6, respectively. Moreover, high content flavonoids. In addition, contained lipids Furthermore, also species-specific metabolites. The yielded 11 specific metabolites, including flavonoids, lipids, others. Meanwhile, fruit-specific were complementary pattern which provides metabolomic evidence healthy diet.

Language: Английский

---

Metabolomics approach for phenolic compounds profiling of soursop (Annona muricata L.) fruit during postharvest storage

Metabolomics, Journal Year: 2024, Volume and Issue: 20(2)

Published: Feb. 25, 2024

Language: Английский

---

Different tools for different trades: contrasts in specialized metabolite chemodiversity and phylogenetic dispersion in fruit, leaves, and roots of the neotropical shrubs Psychotria and Palicourea (Rubiaceae)

Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract Plants produce an astonishingly diverse array of specialized metabolites. A crucial step in understanding the origin such chemodiversity is describing how manifests across spatial and ontogenetic scales relevant to plant–biotic interactions. Focusing on 21 sympatric species Psychotria Palicourea sensu lato (Rubiaceae), we describe patterns metabolite diversity using a combination field collections, untargeted metabolomics, ecoinformatics. We compare α, β, γ metabolites expanding leaves, unripe pulp, immature seed, ripe mature fine roots. Within species, fruit tissues from stages had ≥α than ≤β leaves. Pooled highest all organs, combined higher incidence organ‐specific mass spectral features Roots ≤α leaves lowest β organs. Phylogenetic correlations chemical distance varied by plant organ class. Our results organs provide support for contributions chemodiversity. This study contributes growing within evolutionary ecology biological diversification. Future studies combining our data with biotic interaction experiments can test existing hypotheses roles ecological interactions evolution

Language: Английский

---

Fruit secondary metabolites shape seed dispersal effectiveness

Trends in Ecology & Evolution, Journal Year: 2021, Volume and Issue: 36(12), P. 1113 - 1123

Published: Sept. 8, 2021

Language: Английский

---

Nectar metabolomes contribute to pollination syndromes

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

Summary ‘Pollination syndromes’, where convergent floral signals reflect selection from a functional pollinator group, are often characterized by physical features, yet rewards such as nectar may also pollinators. We asked whether chemistry shows evidence of convergence across groups, i.e. ‘chemical pollination syndrome’. used untargeted metabolomics to compare and leaf chemical profiles 19 bee‐ bird‐syndrome species, focusing on Salvia spp. (Lamiaceae), selected maximize switching events between syndromes. found that independently derived showed traits distinct bee‐syndrome nectar, primarily driven the composition concentration alkaloid profiles. did not find for ‘passive leaking’ compounds leaves since metabolite abundances were uncorrelated tissues many metabolites present in leaves. Nectar metabolomes strongly decoupled phylogenetic relationships within . These results suggest groups drive evolution reward chemistry, consistent with our syndrome’ hypothesis indicative pollinators, but we consider alternative explanations. In addition, support notion can be other tissues.

Language: Английский

---

Tomato plants expressing a stilbene synthase gene display genotype‐depending alterations in metabolome profile and antioxidant system

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(1)

Published: Jan. 1, 2024

Abstract In the present work, we report a study on tomato Micro‐Tom lines expressing Vitis vinifera StSy1 cDNA encoding stilbene synthase under control of CaMV 35S promoter. Transgenic (T1 plants) were evaluated in terms vegetative and reproductive development, fruit production, activity main antioxidant enzymes, metabolome profile both leaves juices. Trans ‐resveratrol glycosylated derivates accumulated some transgenic generated, concentration compounds being higher than Vegetative development was enhanced by transformation, whereas production decreased, soluble solids content (°Bx) increased. Remarkably, trans‐ resveratrol juices showed no correlation with expression levels . An alteration enzyme activities recorded most lines. juices, variation between wild type attributed to major number differentially expressed metabolites as compared that observed leaves. this sense, changes corresponded an induction phenylpropanoid pathway‐related accumulating resveratrol. producing showed, overall, imbalanced metabolism characterized superoxide dismutase (H 2 O ‐producing enzyme) lower H ‐scavenging activities. This work contributes deciphering biochemical consequences introduction opens up prospects for further characterization transformants.

Language: Английский

---