Dynamic distress calls: volatile info chemicals induce and regulate defense responses during herbivory

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: June 19, 2023

Plants are continuously threatened by a plethora of biotic stresses caused microbes, pathogens, and pests, which often act as the major constraint in crop productivity. To overcome such attacks, plants have evolved with an array constitutive induced defense mechanisms— morphological, biochemical, molecular. Volatile organic compounds (VOCs) class specialized metabolites that naturally emitted play important role plant communication signaling. During herbivory mechanical damage, also emit exclusive blend volatiles referred to herbivore-induced (HIPVs). The composition this unique aroma bouquet is dependent upon species, developmental stage, environment, herbivore species. HIPVs from infested non-infested parts can prime responses various mechanisms redox, systemic jasmonate signaling, activation mitogen-activated protein (MAP) kinases, transcription factors; mediate histone modifications; modulate interactions natural enemies via direct indirect mechanisms. These specific volatile cues allelopathic leading altered defense-related genes, viz. , proteinase inhibitors, amylase inhibitors neighboring plants, enhanced levels secondary like terpenoids phenolic compounds. factors deterrents feeding insects, attract parasitoids, provoke behavioral changes their This review presents overview plasticity identified regulators Solanaceous plants. selective emission green leaf (GLVs) including hexanal its derivatives, terpenes, methyl salicylate, (MeJa) inducing during attack phloem-sucking leaf-chewing pests discussed. Furthermore, we focus on recent developments field metabolic engineering focused modulation improve defenses.

Language: Английский

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Metabolomics-Driven Mining of Metabolite Resources: Applications and Prospects for Improving Vegetable Crops

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(20), P. 12062 - 12062

Published: Oct. 11, 2022

Vegetable crops possess a prominent nutri-metabolite pool that not only contributes to the crop performance in fields, but also offers nutritional security for humans. In pursuit of identifying, quantifying and functionally characterizing cellular metabolome pool, biomolecule separation technologies, data acquisition platforms, chemical libraries, bioinformatics tools, databases visualization techniques have come play significant role. High-throughput metabolomics unravels structurally diverse nutrition-rich metabolites their entangled interactions vegetable plants. It has helped link identified phytometabolites with unique phenotypic traits, nutri-functional characters, defense mechanisms productivity. this study, we explore mining metabolites, localizing metabolic pathways, classifying functional biomolecules establishing linkages between fluxes genomic regulations, using comprehensive deciphers plant’s environment. We discuss exemplary reports covering implications metabolomics, addressing changes plants during domestication, stage-dependent growth, fruit development, nutri-metabolic capabilities, climatic impacts, plant-microbe-pest anthropogenic activities. Efforts leading identify biomarker candidate proteins genes responsible plant health, nutri-rich produce are documented. With insights on metabolite-QTL (mQTL) driven genetic architecture, molecular breeding can be revolutionized developing better improved tolerance against diseases/pests enhanced climate resilience

Language: Английский

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Metabolomics‐driven investigation of plant defense response against pest and pathogen attack

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(2)

Published: March 1, 2024

Abstract The advancement of metabolomics has assisted in the identification various bewildering characteristics biological system. Metabolomics is a standard approach, facilitating crucial aspects system biology with absolute quantification metabolites using minimum samples, based on liquid/gas chromatography, mass spectrometry and nuclear magnetic resonance. metabolome profiling narrowed wide gaps missing information enhanced understanding spectrum plant–environment interactions by highlighting complex pathways regulating biochemical reactions cellular physiology under particular set conditions. This high throughput technique also plays prominent role combined analyses plant other omics datasets. Plant opened paradigm opportunities for developing stress‐tolerant plants, ensuring better food quality quantity. However, despite advantageous methods databases, few limitations, such as ineffective 3D capturing metabolites, low comprehensiveness, lack cell‐based sampling. In future, an expansion plant–pathogen plant–pest response towards metabolite architecture necessary to understand intricacies defence against invaders, elucidation metabolic pathway operational during direct correlation between biotic stresses. Our aim provide overview its utilities biomarkers or key associated stress, devising improved diagnostic efficiently assess pest pathogen attack generating crop varieties help application analytical molecular tools.

Language: Английский

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Wheat TaSnRK2.10 phosphorylates TaERD15 and TaENO1 and confers drought tolerance when overexpressed in rice

PLANT PHYSIOLOGY, Journal Year: 2022, Volume and Issue: 191(2), P. 1344 - 1364

Published: Nov. 23, 2022

Abstract Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) acts as a signaling hub in response drought stress, but whether SnRK2 helps plants cope with via other mechanisms largely unknown. Here, we cloned and characterized TaSnRK2.10, which was induced by multiple abiotic stresses phytohormones. Ectopic expression TaSnRK2.10 rice (Oryza sativa) conferred tolerance, manifested improved physiological indices, including increased content, cell membrane stability, survival rates, well decreased loss accumulation H2O2 malonaldehyde. interacted phosphorylated early responsive dehydration 15 (TaERD15) enolase 1 (TaENO1) vivo vitro. TaERD15 prone degradation 26S proteasome, thereby mitigating negative effects on tolerance. Phosphorylation TaENO1 may account for substantially levels phosphoenolpyruvate (PEP), key metabolite primary secondary metabolism, TaSnRK2.10-overexpressing rice, enhancing viability under stress. Our results demonstrate that not only regulated stomatal aperture drought-responsive genes, also enhanced PEP supply promoted TaERD15, all

Language: Английский

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Impact of different cooking methods on the chemical profile of orange-fleshed sweet potato (Ipomoea batatas L.)

LWT, Journal Year: 2022, Volume and Issue: 173, P. 114288 - 114288

Published: Dec. 14, 2022

Orange-fleshed sweet potato (OFSP) has attracted increasing attention due to its high carotenoid levels, which is beneficial human health. However, the changes in chemical composition of OFSP after cooking are not fully elucidated yet. In this study, contents starch, soluble sugar, carotenoids, volatile compounds, and metabolites before (steaming, boiling, baking) were determined evaluate flavor composition. After cooking, starch decreased from 18.15% 7%, sugar increased 11.78% 39.33%. The baked was sweetest one production most sugars during baking. Additionally, aroma best abundance furans, aldehydes, ketones, monoterpenes, including 6 volatiles produced by carotenoids. steamed retained more while all carotenoids reduced 7%–23% cooking. Furthermore, 593 identified OFSP, 82.5% well preserved Overall, understanding profile will provide a theoretical basis for broadening processing application.

Language: Английский

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Transcriptomics and Metabolomics Analyses Reveal High Induction of the Phenolamide Pathway in Tomato Plants Attacked by the Leafminer Tuta absoluta

Metabolites, Journal Year: 2022, Volume and Issue: 12(6), P. 484 - 484

Published: May 26, 2022

Tomato plants are attacked by a variety of herbivore pests and among them, the leafminer Tuta absoluta, which is currently major threat to global tomato production. Although commercial susceptible T. absoluta attacks, better understanding defensive plant responses this pest will help in defining resistance traits broaden range agronomic levers that can be used for an effective integrated management strategy over crop cycle. In study, we developed integrative approach combining untargeted metabolomic transcriptomic analyses characterize local systemic metabolic young larvae herbivory. From analyses, response appeared both systemic, with infested leaves being much more intense than other parts plant. The main was massive accumulation phenolamides great structural diversity, including rare derivatives composed spermine dihydrocinnamic acids. family specialized metabolites supported data, showed induction phenylpropanoid polyamine precursor pathways. Moreover, our data identified two genes strongly induced herbivory, functionally characterized as putrescine hydroxycinnamoyl transferases. They catalyze biosynthesis several phenolamides, caffeoylputrescine. Overall, study provided new mechanistic clues tomato/T. interaction.

Language: Английский

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Integrative transcriptome and metabolome analysis uncovers the Toxoptera aurantii (Hemiptera: Aphididae) response of two Camellia sinensis (Ericales: Theaceae) cultivars

Journal of Economic Entomology, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

The tea aphid Toxoptera aurantii Boyer (Hemiptera: Aphididae) is a destructive pest that infests plants. resistance mechanisms of the plant against T. infestation are largely unexplored. This study investigates defensive response plants to feeding using an aphid-resistant Camellia sinensis cultivar 'Qiancha1' (QC1) and aphid-susceptible C. 'Huangjinya' (HJY). Transcriptomics metabolomics analyses were conducted on 4 samples: QCCK (T. non-infested QC1), HJYCK HJY), QC24 aurantii-infested QC1 for 24 h), HJY24 HJY h). results showed differentially expressed genes (DEGs) accumulated metabolites (DAMs) in 2 comparison groups (QCCK vs. HJY24) primarily enriched metabolic pathways, including hormone signal transduction, phenylpropanoid biosynthesis flavonoid biosynthesis. Following infestation, resistant exhibited more DEGs DAMs than susceptible HJY, indicating stronger stress. Additionally, expression phenylpropanoid- flavonoid-related (CYP, 4CL, FLS, F3H, LAR) was significantly upregulated compared with HJY. Metabolites involved phenylpropanoid/flavonoid such as p-coumaroyl-CoA, caffeoylquinic acid, feruloyl-CoA, exclusively induced QC1. These findings suggest pathways play pivotal roles providing valuable insights breeding utilization germplasms.

Language: Английский

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Chemical bonding of cross-linked glutaraldehyde/chitosan on the surface of a titanium wire to prepare a robust biocompatible SPME fiber for analysis of phytohormones in plants

Food Chemistry, Journal Year: 2024, Volume and Issue: 449, P. 139168 - 139168

Published: March 30, 2024

Language: Английский

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Genome-Wide Identification, Evolution, and Female-Biased Expression Analysis of Odorant Receptors in Tuta absoluta (Lepidoptera: Gelechiidae)

Life, Journal Year: 2024, Volume and Issue: 14(7), P. 872 - 872

Published: July 12, 2024

The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a highly destructive invasive pest targeting Solanaceae crops. Its olfactory system plays crucial role in host location, mate finding, and other behavioral activities. However, there notable gap the literature regarding characterization of its chemosensory genes. In this study, we conducted genome-wide identification 58 odorant receptors (ORs) T. absoluta. identified ORs exhibit coding sequence (CDS) lengths ranging from 1062 bp to 1419 bp, encoding proteins 354 473 amino acids. Gene structure analysis showed that majority these consist five, seven, eight, or nine exons, collectively representing 67% total identified. Through chromosomal mapping, several tandemly duplicate genes, including TabsOR12a, TabsOR12b, TabsOR12c, TabsOR21a, TabsOR21b, TabsOR34a, TabsOR34b, TabsOR34c, TabsOR62a, TabsOR62b. phylogenetic indicated six TabsORs were clustered within lepidopteran sex pheromone receptor clade, while an expansion clade containing ten resulted tandem duplication events. Additionally, five classified into specific OR Furthermore, through RNA-Seq RT-qPCR analyses, (TabsOR21a, TabsOR26a, TabsOR36) exhibiting female-antennae-biased expression. Our study provides valuable foundation further investigations molecular ecological functions TabsORs, particularly relation oviposition behavior. These findings provide foundational data for future exploration female-biased expression genes absoluta, thereby facilitating development eco-friendly attract-and-kill techniques prevention control

Language: Английский

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A Compendium for Novel Marker-Based Breeding Strategies in Eggplant

Plants, Journal Year: 2023, Volume and Issue: 12(5), P. 1016 - 1016

Published: Feb. 23, 2023

The worldwide production of eggplant is estimated at about 58 Mt, with China, India and Egypt being the major producing countries. Breeding efforts in species have mainly focused on increasing productivity, abiotic biotic tolerance/resistance, shelf-life, content health-promoting metabolites fruit rather than decreasing anti-nutritional compounds fruit. From literature, we collected information mapping quantitative trait loci (QTLs) affecting eggplant’s traits following a biparental or multi-parent approach as well genome-wide association (GWA) studies. positions QTLs were lifted according to reference line (v4.1) more 700 identified, here organized into 180 genomic regions (QGRs). Our findings thus provide tool to: (i) determine best donor genotypes for specific traits; (ii) narrow down QTL by combining from different populations; (iii) pinpoint potential candidate genes.

Language: Английский

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