Genome-wide Identification and Expression Analysis of the ADH Gene Family in Tobacco (Nicotiana tabacum L.) DOI Creative Commons
Ruiqi Wang,

Chaofan Du,

Gang Gu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 31, 2023

Abstract Background Alcohol dehydrogenases (ADHs) are the crucial enzymes involved in plant development, biotic and abiotic stresses. Tobacco is one of most important model plants, which will be great application significance to analyze ADH gene family expression profile under various stresses tobacco. Results A total 53 genes were identified tobacco ( Nicotiana tabacum L.) genome grouped into 6 subfamilies based on phylogenetic analysis. Gene structure (exon/intron) protein motifs highly conserved among NtADH genes, especially members within same subfamily. 5 pairs tandem duplication, 3 segmental duplication analysis events. Cis -regulatory elements promoters participated cell hormones, environmental stress, light responsiveness. Expression showed that widely expressed different maturity leaves, as well leaves after topping, however, patterns appeared diversed. The qRT-PCR 13 confirmed their differential response bacterial pathogen Ralstonia solanacearum L. infection. Metabolomics revealed primarily associated with carbohydrate metabolism, moreover, four NtADH20/24/48/51 ) notably pathway alpha-linolenic acid metabolism. Conclusion genome-wide identification, evolutionary have provided valuable insights role these Our results also laid a solid foundation for further functional study tobacco, understanding NtADHs more stress conditions.

Language: Английский

Molecular mechanism of drought resistance in soybean roots revealed using physiological and multi-omics analyses DOI
Xiyue Wang, Wei Zhao,

Xinhe Wei

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 208, P. 108451 - 108451

Published: Feb. 20, 2024

Language: Английский

Citations

17
Genome-wide identification of SWEET family genes and functional analysis of NtSWEET12i under drought and saline-alkali stresses in tobacco DOI Creative Commons
Wenting Song, Luyao Xue,

Xiaoshan Jin

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 14, 2025

SWEET (Sugar Will Eventually be Exported Transporter) proteins play vital roles in the transport of sugars, contributing to regulation plant development, hormone signaling, and responses abiotic stress. In this study, we identified 57 NtSWEETs tobacco (Nicotiana tabacum L.), then their physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter cis-elements, protein interaction network, tissue specificity, expression pattern were systematically analyzed. addition, NtSWEET12i improves drought saline-alkali tolerance by enhancing soluble sugars transport, ABA proline biosynthesis, ROS scavenging. These findings illuminate role NtSWEETs, particularly NtSWEET12i, regulating stresses. This study offers new insights enhance our understanding identify potential genes involved plants. Not applicable.

Language: Английский

Citations

1
Characteristics of NAC transcription factors in Solanaceae crops and their roles in responding to abiotic and biotic stresses DOI

Xiaogang Ou,

Lixinyu Sun,

Yu Chen

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 709, P. 149840 - 149840

Published: March 28, 2024

Language: Английский

Citations

5
Genome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco ( Nicotiana tabacum L.) DOI Creative Commons
Zhen Tian, Luyao Xue,

Jincun Fu

et al.

Plant Signaling & Behavior, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 16, 2025

Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles the embryonic development, photosynthesis, flowering, abiotic stress responses, other essential processes plants. To better understand genome-wide NF-Y domain-containing proteins, protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter cis-elements, interaction network NtNF-Ys tobacco (Nicotiana tabacum L.) were systematically analyzed. In this study, we identified 58 tobacco, respectively, divided into subfamilies corresponding to their relationships. Their tissue specificity expression pattern analyses for leaf drought saline-alkali stress, ABA response carried out using RNA-seq or qRT-PCR. These findings illuminate role regulating plant tolerance, response. This study offers new insights enhance our understanding identify potential genes involved as well tolerance

Language: Английский

Citations

0
Genome-wide identification and expression analysis of the ADH gene family in Artemisia annua L. under UV-B stress DOI Creative Commons
Hengyu Pan, Peiqi Shi, Shan Zhong

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

ADHs are key genes that catalyze the interconversion between alcohols and aldehydes, which play crucial roles in plant adaptation to a range of abiotic stresses. However, characterization evolutionary pathways ADH antimalarial Artemisia annua still unclear. This study identified 49 A. conducted detailed analysis their structural features, conserved motifs, duplication types, revealing tandem dispersed duplications primary mechanisms gene expansion. Evolutionary ( AanADH ) argyi AarADH revealed dynamic changes, with 35 deriving from most recent common ancestor both species. ADH1 , for artemisinin production, had two copies species, expanding via but whole-genome . CREs WGCNA suggested may be regulated by UV-B stress. Following short-term treatment, 16 DEGs were identified, including AanADH6 AanADH7 ), these significantly downregulated after hours treatment (UV2h) upregulated four (UV4h). The expression changes further confirmed GO enrichment qRT-PCR experiments. Overall, this comprehensively characterized family systematically responsive stress, providing foundation research on stress responses.

Language: Английский

Citations

0
Integrated transcriptomics and metabolomics provides insights into the Nicotiana tabacum response to heat stress DOI Creative Commons
Liqiao Chen,

Shaoxin Qiu,

Yuanping Chen

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: July 22, 2024

Heat stress is a prevalent factor that significantly damages crops, especially with the ongoing global warming and increasing frequency of extreme weather events. Tobacco particularly sensitive to temperature fluctuations, experiencing reduced yield quality under high temperatures. However, underlying molecular mechanisms heat resistance in tobacco remain poorly understood. This study comprehensively analyzed biochemical, transcriptomic, metabolomic responses on root shoot cultivar K326 compared control conditions. increased activities antioxidant enzymes (CAT, POD, SOD) levels osmotic mediators (soluble sugars, sucrose, proline) shoot. Furthermore, transcriptome analysis identified 13,176 differentially expressed genes (DEGs) (6,129 up-regulated 7,047 down-regulated) 12,283 DEGs (6,621 5,662 The had 24 enriched KEGG pathways, including phenylpropanoid metabolism, while 32 significant such as galactose metabolism MAPK signaling. data 647 metabolites 932 shoot, carbohydrates amino acids being main categories. 116 abundant (DAMs) (107 9 down-regulated), contained 256 DAMs (251 5 down-regulated). Joint metabolome showed starch sucrose were co-enriched both tissues. In contrast, sugar nucleotide was root, purine metabolic pathway can modulate expression MYB transcription factors by influencing ABA synthesis signaling, thereby controlling accumulation HSPs, raffinose, trehalose enhance tolerance. NtMYB78 , an factor, enhances tolerance for tobacco. research offers foundational framework investigating implementing heat-resistant pathways seedlings.

Language: Английский

Citations

3
Tobacco production under global climate change: combined effects of heat and drought stress and coping strategies DOI Creative Commons
Ming Liu, Xingjun Liu,

Yuxiao Song

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 26, 2024

With the intensification of global climate change, high-temperature and drought stress have emerged as critical environmental stressors affecting tobacco plants’ growth, development, yield. This study provides a comprehensive review tobacco’s physiological biochemical responses to optimal temperature conditions limited irrigation across various growth stages. It assesses effects these on yield quality, along with synergistic interactions molecular mechanisms associated stressors. High-temperature induces alterations in both enzymatic non-enzymatic antioxidant activities, lead accumulation reactive oxygen species (ROS), promote lipid peroxidation, all which adversely impact processes such photosynthetic gas exchange, respiration, nitrogen metabolism, ultimately resulting reduced biomass, productivity, quality. The interaction activates novel plant defense mechanisms, contributing exacerbated damage. Optimal enhance activation heat shock proteins (HSPs) antioxidant-related genes at level. At same time, water triggers expression regulated by abscisic acid-dependent independent signaling pathways. also discusses contemporary agricultural management strategies, applications genetic engineering, biotechnological breeding methods designed mitigate adverse agroclimatic responses, focusing enhancing production under conditions.

Language: Английский

Citations

3
Integrative physiological, metabolomic and transcriptomic insights into phenylpropanoids pathway responses in Nicotiana tabacum under drought stress DOI Creative Commons

Quanyu Yin,

Feng Zhao,

Zhichao Ren

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100815 - 100815

Published: March 1, 2025

Language: Английский

Citations

0
Genotypic differences in morphological, physiological and agronomic traits in response to drought in tobacco genotypes DOI Creative Commons

Shou‐Heng Shi,

Cheng-Wei Qiu,

Paul Holford

et al.

Plant Growth Regulation, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Language: Английский

Citations

0
Genome-wide identification and expression analysis of the ADH gene family under diverse stresses in tobacco (Nicotiana tabacum L.) DOI Creative Commons
Ruiqi Wang,

Chaofan Du,

Gang Gu

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 2, 2024

Abstract Background Alcohol dehydrogenases (ADHs) are the crucial enzymes that can convert ethanol into acetaldehyde. In tobacco, members of ADH gene family involved in various stresses tolerance reactions, lipid metabolism and pathways related to plant development. It will be great application significance analyze expression profile under tobacco. Results A total 53 genes were identified tobacco ( Nicotiana tabacum L . ) genome grouped 6 subfamilies based on phylogenetic analysis. Gene structure (exon/intron) protein motifs highly conserved among NtADH genes, especially within same subfamily. 5 pairs tandem duplication, 3 segmental duplication analysis events. Cis -regulatory elements promoters participated cell development, hormones, environmental stress, light responsiveness. The showed widely expressed topping stress leaf senescence. However, patterns different appeared diverse. qRT-PCR 13 displayed their differential pattern response bacterial pathogen Ralstonia solanacearum infection. Metabolomics revealed primarily associated with carbohydrate metabolism, moreover, four NtADH20/24/48/51 notably pathway alpha-linolenic acid which up-regulation 9-hydroxy-12-oxo-10(E), 15(Z)-octadecadienoic 9-hydroxy-12-oxo-15(Z)-octadecenoic acid. Conclusion genome-wide identification, evolutionary analysis, profiling, exploration metabolites metabolic have yielded valuable insights roles these stresses. Our results could provide a basis for functional stressful conditions.

Language: Английский

Citations

3