Rhizosheath drought responsiveness is variety‐specific and a key component of belowground plant adaptation

New Phytologist, Journal Year: 2024, Volume and Issue: 242(2), P. 479 - 492

Published: Feb. 28, 2024

Summary Biophysicochemical rhizosheath properties play a vital role in plant drought adaptation. However, their integration into the framework of response is hampered by incomplete mechanistic understanding responsiveness and unknown linkage to intraspecific plant–soil reactions. Thirty‐eight Zea mays varieties were grown under well‐watered conditions assess properties, such as soil aggregation, mass, net‐rhizodeposition, organic carbon distribution. Additionally, explanatory traits, including functional trait adaptations changes enzyme activities, measured. Drought restricted structure formation shifted plant–carbon from litter‐derived matter macroaggregates microbially processed compounds microaggregates. Variety‐specific modifications determined variations responsiveness. responses system ranged among maintaining plant–microbial interactions through accumulation rhizodeposits, preserving while increasing exploration enhanced root elongation. Drought‐induced alterations at root–soil interface may hold crucial implications for ecosystem resilience changing climate. Our findings highlight that are an intrinsic component response, emphasizing need holistic concept systems future research on

Language: Английский

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Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 15, 2024

Pinus taeda is an important forest tree species for plantations because of its rapid growth and high yield oleoresins. Although P. distribute in warm wet southern China, drought, sometime serious long time, often occurs the region. To explore drought tolerance usage beneficial microorganisms, seedlings were planted pots inoculated with root endophytic fungus Serendipita indica finally treated stress 53 d. Metabolome proteome their needles analyzed. The results showed that S. inoculation under caused great changes levels some metabolites needles, especially flavonoids organic acids. Among them, eriocitrin, trans -aconitic acid, vitamin C, uric alpha-ketoglutaric A, stachydrine, coumalic itaconic calceolarioside B, 2-oxoglutaric citric acid upregulated more than three times stress, compared to those non-inoculated stress. KEGG analysis pathways enriched such as flavonoid biosynthesis, ascorbate aldarate metabolism, C5-branched dibasic metabolism. Proteome revealed specific differential proteins. Two proteins, namely, H9X056 H9VDW5, only appeared protein H9VNE7 was 11.0 In addition, increased enrichment water deficient-inducible proteins (such LP3-1, LP3-2, LP3-3, dehydrins) involved ribosomal structures A0A385JF23). Meanwhile, biosynthesis metabolism pathways, mainly including phenylpropanoid cutin, suberine wax 2-oxocarboxylic there positive relationships between accumulation Altogether, our metabolome provided a guideline further study functions related

Language: Английский

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Plant growth promoting bacteria (PGPB)-induced plant adaptations to stresses: an updated review

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17882 - e17882

Published: Aug. 20, 2024

Plants and bacteria are co-evolving interact with one another in a continuous process. This interaction enables the plant to assimilate nutrients acquire protection help of beneficial known as growth-promoting (PGPB). These naturally produce bioactive compounds that can assist plants’ stress tolerance. Moreover, they employ various direct indirect processes induce growth protect plants against pathogens. The mechanisms involve phytohormone production, phosphate solubilization, zinc potassium ammonia nitrogen fixation while, production siderophores, lytic enzymes, hydrogen cyanide, antibiotics included under mechanisms. property be exploited prepare bioformulants for biofertilizers, biopesticides, biofungicides, which convenient alternatives chemical-based products achieve sustainable agricultural practices. However, application importance PGPB agriculture still debatable despite its immense diversity growth-supporting activities. performance varies greatly is dictated by environmental factors affecting development. review emphasizes role activities (stress tolerance, phytohormones) summarises new formulations opportunities.

Language: Английский

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Ethylene: A Master Regulator of Plant–Microbe Interactions under Abiotic Stresses

Cells, Journal Year: 2022, Volume and Issue: 12(1), P. 31 - 31

Published: Dec. 21, 2022

The plant phytohormone ethylene regulates numerous physiological processes and contributes to plant–microbe interactions. Plants induce production ward off pathogens after recognition of conserved microbe-associated molecular patterns (MAMPs). However, immune responses against are essentially not different from those triggered by neutral beneficial microbes. Recent studies indicate that is an important factor for plant–microbial association under abiotic stress such as salt heat stress. microbes with plants stresses modulates levels which control the expression ethylene-responsive genes (ERF), ERFs further regulate transcriptome, epi-transcriptome, Na+/K+ homeostasis antioxidant defense mechanisms reactive oxygen species (ROS). Understanding ethylene-dependent interactions crucial development new strategies aimed at enhancing tolerance harsh environmental conditions. In this review, we underline importance in interaction stresses.

Language: Английский

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Profiling of Volatile Organic Compounds from Four Plant Growth-Promoting Rhizobacteria by SPME–GC–MS: A Metabolomics Study

Metabolites, Journal Year: 2022, Volume and Issue: 12(8), P. 763 - 763

Published: Aug. 19, 2022

The rhizosphere microbiome is a major determinant of plant health. Plant-beneficial or growth-promoting rhizobacteria (PGPR) influence growth, development and adaptive responses, such as induced resistance/priming. These new eco-friendly choices have highlighted volatile organic compounds (biogenic VOCs) potentially inexpensive, effective efficient substitute for the use agrochemicals. Secreted bacterial VOCs are low molecular weight lipophilic with boiling point high vapor pressures. As such, they can act short- long-distance signals in rhizosphere, affecting competing microorganisms impacting In this study, secreted from four PGPR strains (Pseudomonas koreensis (N19), Ps. fluorescens (N04), Lysinibacillus sphaericus (T19) Paenibacillus alvei (T22)) were profiled by solid-phase micro-extraction gas chromatography mass spectrometry (SPME–GC–MS) combined multivariate data analysis. Metabolomic profiling chemometric analyses revealed novel on composition VOC blends strains. Of 121 annotated metabolites, most known bioactives which able to affect metabolism hosts. belong following classes: alcohols, aldehydes, ketones, alkanes, alkenes, acids, amines, salicylic acid derivatives, pyrazines, furans, sulfides terpenoids. results further demonstrated presence species-specific strain-specific VOCs, characterized either absence specific different molecules could be investigated biomarkers classification an organism selection agricultural use.

Language: Английский

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Genetic dissection of drought resistance for trait improvement in crops

The Crop Journal, Journal Year: 2023, Volume and Issue: 11(4), P. 975 - 985

Published: June 22, 2023

Reliance on agriculture for food security is a constant in all modern societies. Global climate change and population growth have put immense pressure sustainable agriculture, exacerbating the effects of environmental stresses. Drought one most pressing abiotic stresses that farmers face, presenting an annual threat to crop yield. Crops evolved extensive morphological, physiological, molecular mechanisms combat drought stress. resistance polygenic trait, controlled by complex genetic network array genes working together ensure plant survival. Many studies aimed at dissecting underlying resistance. Recent using linkage association mapping made progress identifying variations affect drought-resistance traits. These loci may potentially be engineered transformation genome editing developing new, stress-resistant cultivars. Here we summarize recent elucidating basis Molecular-breeding technologies such as marker-assisted selection, gene transformation, are currently employed develop drought-resistant germplasm variety crops. advances basic research biotechnology covered this review will facilitate delivery crops with unprecedented efficiency.

Language: Английский

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Bacillus subtilis ER-08, a multifunctional plant growth-promoting rhizobacterium, promotes the growth of fenugreek (Trigonella foenum-graecum L.) plants under salt and drought stress

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 24, 2023

Sustainable agriculture and meeting the world's food needs face considerable obstacles from abiotic stresses such as soil salinity drought. This critical issue was addressed by our current study, which sought to uncover multi-trait bioinoculants hostile ecosystems that could help mitigate drought at same time.The Bacillus subtilis ER-08 (BST) strain isolated halotolerant plant Fagonia cretica collected Little Rann of Kachchh, India. Various biochemical molecular approaches were applied for detailed characterization BST isolate.The isolate demonstrated notable growth-promoting qualities. Fenugreek seed biopriming performed using isolate. The effect treatment on fenugreek developmental indices well alleviation examined under greenhouse conditions. produced 83.7 g ml-1 gibberellins (GA3) 176.1 indole-3 acetic acid. Moreover, hydrogen cyanide, siderophore, exopolysaccharides (EPS), ammonia, cellulase, protease, pectinase, chitinase also strain. Interestingly, 52% Fusarium oxysporum mycelial growth suppressed in vitro Furthermore, isolates functioned several stress conditions, instance, (4 6 ds m-1), pH (5, 7, 9), (PEG6000 10%, 20%, 30%), temperature (25°C, 35°C, 37°C, 55°C). study indicates might serve an effective bio-inoculant minimizing detrimental effects stresses.

Language: Английский

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Growth enhancement and extenuation of drought stress in maize inoculated with multifaceted ACC deaminase producing rhizobacteria

Frontiers in Sustainable Food Systems, Journal Year: 2023, Volume and Issue: 6

Published: Jan. 13, 2023

Introduction Maize is a major staple cereal crop grown and consumed globally. However, due to climate change, extreme heat drought stresses are greatly affecting its production especially in sub-Saharan Africa. The use of bio-based approach mitigate stress therefore suggested using plant growth-promoting rhizobacteria (PGPR). Methods This study investigated the abilities 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR Pseudomonas sp. MRBP4, MRBP13 Bacillus MRBP10 isolated from maize rhizosphere soil, ameliorate effect genotypes MR44 S0/8/W/I137TNW//CML550 under two water regimes; mild (50% FC) well-watered conditions (100% FC). rhizobacterial strains were identified by 16S rRNA sequencing biochemical tests, evaluated for abiotic tolerance traits. Results discussion synergistic bacterial had highly significant ( p < 0.001) on total soluble sugar, soil moisture content relative content, which enhanced water-stress inoculated plants. Relative was significantly highest plants co-inoculated with MRBP4 + (60.55%). Total chlorophyll seedlings sole MRBP13, 15.91%, 14.99% 15.75% respectively, over un-inoculated control. Soil increased 28.67% 30.71% compared control when respectively. interactive genotype × bacteria biomass production. Leaf area (212.45 ± 0.87 cm 2 ) stress. Treatment seeds MRBP 4 root length (10.32 0.48 cm) survival seedlings. Bioinoculation these could boost cultivated arid regions.

Language: Английский

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Efficacy of endophytic bacteria as promising inducers for enhancing the immune responses in tomato plants and managing Rhizoctonia root-rot disease

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 15, 2024

Abstract Around the world, a variety of crops, including tomatoes, suffer serious economic losses due to Rhizoctonia root-rot disease. Herein, Bacillus velezensis , megaterium, and Herpaspirillum huttiense isolated from strawberry (Fragaria chiloensis var. ananassa) plants were pragmatic as plant growth promotors for battling root rot disease bringing about defense mechanisms well promotional strategies in tomato . These endophytic bacteria demonstrated potent antifungal activity against R. solani vitro along vivo. Data explained that could produce Indole acetic acid, Gibberellic acid GA, siderophore solubilize phosphate soil. The consortium ( ) increased protection % infection by (79.4%), followed B. (73.52%), H. (70.5%), megaterium (67.64%), respectively. There was an increase soluble proteins carbohydrates infected treated with 30.7% 100.2% over untreated plants, Applying either alone or combination lowered level malondialdehyde MDA hydrogen peroxide H 2 O improved activities antioxidant enzymes both uninfected plants. Also, bacterial endophytes have distinctive reactions regarding number concentrations isozymes It be recommended commercial usage mixture targeted endophyte strains therapeutic nutrients inducer.

Language: Английский

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Biofilm producing plant growth promoting bacteria in combination with glycine betaine uplift drought stress tolerance of maize plant

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 9, 2024

Introduction The escalating threat of drought poses a significant challenge to sustainable food production and human health, as water scarcity adversely impacts various aspects plant physiology. Maize, cornerstone in staple cereal crops, faces the formidable stress that triggers series transformative responses plant. Methods present study was carried out two sets experiments. In first experiment, applied after maintaining growth for 45 days then irrigation skipped, samples were collected at 1 st , 3 rd 6 th day interval evaluation changes growth, relation (relative content) antioxidants activity by inoculating indigenously isolated tolerant biofilm producing rhizobacterial isolates ( Bacillus subtilis SRJ4, Curtobacterium citreum MJ1). second glycine betaine osmoregulator addition PGPR perceive modulation photosynthetic pigments (Chlorophyll b) under varying moisture levels (100, 75 50% FC). Results discussion revealed upsurge root shoot length, fresh dry biomass besides increasing chlorophyll contents stressed inoculated plants compared uninoculated plants. Glycine application resulted an additional boost pigments, when combination with bacterial inoculants. However, both inoculants behaved differently evident from their biochemical physiological attributes. Isolate SRJ4 proved be superior its potential express antioxidant activity, leaf relative responsive gene expression while isolate MJ1 showed exclusive increase P contents. Though it is quite difficult having promoting traits tolerance together yet, such biological resources could exceptional option improving crop productivity agriculture abiotic stresses. By exploring combined betaine, seeks provide insights into strategies developing agricultural practices aimed resilience challenging environmental conditions.

Language: Английский

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