Analysis of tea quality of large-leaf black tea with different harvesting tenderness based on metabolomics

Food Control, Journal Year: 2024, Volume and Issue: 163, P. 110474 - 110474

Published: April 4, 2024

Language: Английский

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Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 803 - 803

Published: Jan. 18, 2025

Late-maturing hybrid citrus is a significant fruit that combines the best traits of both parents and highly prized for its unique flavor. Not only can organic acids alter flavor pulp, but they are also essential cellular metabolism, energy conversion, maintaining acidbase balance in plant tissues. Although play key role quality formation fruits, there still insufficient research on metabolic processes late-maturing varieties. In this study, three varieties with different acidity levels, namely ‘Huangjinjia’ (HJ), ‘Kiyomi’ (QJ), ‘Harumi’ (CJ), were selected to systematically investigate regulation mechanism through transcriptome sequencing technology, combined physiological biochemical analyses. This study revealed gene expression differences related acid synthesis degradation. Through profiling, several genes closely associated metabolism identified, preliminary network was constructed. The results showed pathways between growth stages fruit. Specifically, HJ had higher TA content than QJ CJ, primarily due significantly citric malic contents compared other two Further analysis four modules high correlation levels major fruits. involved these synthesis, degradation, transport. Additionally, we identified (AS1, BZP44, COL4, TCP4, IDD10, YAB2, GAIPB) might be metabolism. functions could have impact changes enzymes provides foundation exploring intrinsic mechanisms regulating fruits contributes functional molecular design high-quality

Language: Английский

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Integrated transcriptomic and metabolomic analyses elucidate the mechanism by which grafting impacts potassium utilization efficiency in tobacco

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 23, 2025

Potassium plays a crucial role in determining the quality of flue-cured tobacco leaves. Our prior investigations have demonstrated that using potassium-efficient rootstocks through grafting offers viable solution to prevalent issue low potassium levels Chinese Nevertheless, specific molecular mechanisms responsible for increase content following leaves yet be elucidated. This study revealing first time how improves utilization efficiency combined transcriptome and metabolome analysis. selected Wufeng NO. 2, variety, Yunyan 87, main cultivar, as research subjects investigate underlying reasons differential among different metabolic data analysis grafted tobacco. The results showed considerable increment 90.1% Overall, 2044 differentially expressed genes were identified analysis, with majority being enriched plant hormone signal transduction MAPK pathway. Metabolome revealed 175 metabolites significant differences, primarily involving primary such amino acids carbohydrates. Among these, there was an related glycolysis, acid metabolism, TCA cycle pathway key above pathways Mantel-Pearson correlation leading identification 2 3 metabolites, including IAA, CIP1, D-fructose, Fumaric Oxoglutaric acid, significantly associated increased uncovers intricate mechanism behind enhance efficiency, thereby offering theoretical support enhancing crop nutrient technology.

Language: Английский

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The key metabolic genes and networks regulating the fruit acidity and flavonoid of Prunus mume revealed via transcriptomic and metabolomic analyses

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 31, 2025

The acidic taste of Mei fruit (Prunus mume) is a major contributor to its quality, but formation mechanism remains unclear. Here, we unraveled the networks organic acid and flavonoid metabolism in two fruit. results showed that differentially expressed genes were mainly concentrated processes carbohydrate derivative binding, carboxylic acid, metabolism. While accumulated metabolites associated with flavone flavonol biosynthesis amino carbon Moreover, identified key metabolites, such as citric succinic acids, which may be central development acidity fruit, determined they are under regulatory influence specific genes, including galactinol-sucrose-galactosyltransferase 5, mitogen-activated protein kinase NPK1-like, glutamate receptor, chalcone isomerase. Furthermore, transcription factors ERF027, bHLH92, bHLH35, WRKY23 potential pivotal regulators within these networks. These provide new insights into metabolic regulation

Language: Английский

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Metabolomic and transcriptomic analyses reveal the formation mechanism of bitter components in blue honeysuckle

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117727 - 117727

Published: March 1, 2025

Language: Английский

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Integrated Transcriptomic and Metabolomic Analyses Identify Critical Genes and Metabolites Associated with Seed Vigor of Common Wheat

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 526 - 526

Published: Dec. 30, 2023

Seed aging is a common physiological phenomenon during storage which has great impact on seed quality. An in-depth analysis of the and molecular mechanisms wheat significance for cultivating high-vigor varieties. This study reveals in two cultivars differing vigor, combining metabolome transcriptome analyses. Differences between were examined based metabolomic differential analysis. Artificial had significant metabolism seeds. A total 7470 (3641 upregulated 3829 downregulated) DEGs detected non-aging HT LT seeds; however, 10,648 (4506 up 6142 down) after treatment. Eleven, eight, four key metabolic-related gene families identified glycolysis/gluconeogenesis TCA cycle pathways, starch sucrose galactose respectively. In addition, 111 up-regulated transcription factor genes 85 down-regulated 48h group. 548 metabolites across all samples. Cultivar comparisons non-aged groups aged revealed 46 (30 16 62 (38 24 DIMs, Network indicated that glucarate O-phosphoric acid, L-methionine sulfoxide, isocitric Gln-Gly might be most crucial DIMs LT. The main related enriched pathways such as glyoxylate dicarboxylate metabolism, biosynthesis secondary metabolites, fatty acid degradation, etc. However, exhibited differences mainly carbon cycle, Through combined analyses, it was found artificial significantly affected glycolysis/gluconeogenesis, pyruvate involved ACS, F16P2, PPDK1. We thus speculate these may regulating changes seeds aging. an cultivar to amino polypeptide cysteine methionine glutathione sugar nucleotide involving BCAT3, CHI1, GAUT1, GAUT4, play pivotal roles vigor cultivars.

Language: Английский

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Transcription factor AcAGL9 and AcBEH1 synergistically regulate carotenoid biosynthesis during pineapple fruit ripening

Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 222, P. 113385 - 113385

Published: Jan. 9, 2025

Language: Английский

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Phyto Constituents of Ananas comosus Leaf Extract Enhancing the Copper Nanoparticles Synthesis

Pertanika journal of science & technology, Journal Year: 2025, Volume and Issue: 33(3)

Published: April 4, 2025

Ananas comosus leaf is one of the agricultural wastes that has resulted in environmental pollution. Recently, this waste been explored for its potential synthesising environmentally friendly metallic nanoparticles. extract proposed as a viable and economical option reducing, capping, stabilising agents copper This study used sulphate precursor materials to produce nanoparticles green synthesis process. The results show absorption spectra UV-visible range exhibit peak value at wavelength 238 nm. Fourier Transform Infrared analysis revealed presence bioactive compounds extract, which are responsible reducing Cu²⁺ ions Cu⁰ Field Emission Scanning Electron Microscope indicated have face-centred cubic crystal structure, significant innovative nanoparticle applications. average particle size was determined be 56.71 X-ray diffraction peaks 43.46°, 50.57°, 74.23°, corresponding (111), (200), (220) lattice planes, respectively, confirming structural properties synthesised Energy Dispersive examination found primarily composed copper, accounting 95.24% total composition. phytochemicals effectively utilised nanoparticles, demonstrating applications medicine agriculture.

Language: Английский

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Effects of chitosan and acetic acid composite coating on the quality and metabolomic characteristics of postharvest pineapple

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144488 - 144488

Published: April 1, 2025

Language: Английский

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Integrative transcriptome and metabolome analysis reveals the mechanisms of light-induced pigmentation in purple waxy maize

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 15, 2023

Waxy maize, mainly consumed at the immature stage, is a staple and vegetable food in Asia. The pigmentation kernel of purple waxy maize enhances its nutritional market values. Light, critical environmental factor, affects anthocyanin biosynthesis results different parts plants, including kernel. SWL502 light-sensitive inbred line with color, but regulatory mechanism resulting color still unknown.In this study, cyanidin, peonidin, pelargonidin were identified as main components SWL502, evaluated by ultra-performance liquid chromatography (UPLC) method. Investigation pigment accumulation was performed 12, 17, 22 days after pollination (DAP) under both dark light treatment conditions via transcriptome metabolome analyses.Dark affected genes metabolites associated metabolic pathways amino acid, carbohydrate, lipid, galactose, phenylpropanoid terpenoid backbone, ABC transporters. expression genes, such 4CL2, CHS, F3H, UGT, reduced treatment. Dynamic changes time-series analysis. involved photosynthesis purine metabolism altered treatment, carotenoid biosynthesis, sphingolipid metabolism, MAPK signaling pathway, plant hormone signal transduction pathway induced Light increased level major transcription factors LRL1, myc7, bHLH125, PIF1, BH093, PIL5, MYBS1, BH074 kernels, while greatly promoted RVE6, MYB4, MY1R1, MYB145.This study first report to investigate effects on underlying levels, from are valuable for future research better understand regulation growth.

Language: Английский

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