Characterization of regulatory genes Plhffp and Plpif1 involved in conidiation regulation in Purpureocillium lavendulum DOI Creative Commons
Wei Yu,

Feng-Na Qi,

Yanrui Xu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 15, 2024

Purpureocillium lavendulum is an important biocontrol agent against plant-parasitic nematodes, primarily infecting them with conidia. However, research on the regulatory genes and pathways involved in its conidiation still limited. In this study, we employed Agrobacterium tumefaciens -mediated genetic transformation to generate 4,870 random T-DNA insertion mutants of P. . Among these mutants, 131 strains exhibited abnormal conidiation, further in-depth investigations were conducted two (designated as #5-197 #5-119) that showed significantly reduced conidiation. Through whole-genome re-sequencing genome walking, identified sites determined corresponding affected by insertions, namely Plhffp Plpif1 Both knocked out through homologous recombination, phenotypic analysis revealed a significant difference between knockout wild-type strain ( ku80 ). Upon complementation Δ wildtype allele, was restored level comparable , providing evidence involvement gene regulation The or antioxidant capacity absence also resulted decreased resistance SDS, suggesting may be integrity cell wall. RT-qPCR altered expression levels several known associated Additionally, nematode infection assays Caenorhabditis elegans indicated indirectly pathogenicity towards nematodes. results demonstrate - mediated mutagenesis, knockout, can highly effective for identifying functionally

Language: Английский

Biosecurity risks to human food supply associated with plant-parasitic nematodes DOI Creative Commons
Camelia Kantor, J. D. Eisenback, Mihail R. Kantor

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: April 30, 2024

Biosecurity in agriculture is essential for preventing the introduction and spread of plant-parasitic nematodes (PPNs) which threaten global food security by reducing crop yields facilitating disease spread. These risks are exacerbated increased trade climate change, may alter PPN distribution activity, increasing their impact on agricultural systems. Addressing these challenges vital to maintaining integrity supply chain. This review highlights significant advancements managing PPN-related biosecurity within chain, particularly considering change’s evolving influence. It discusses modes transmission, factors risk infestation, PPNs safety security, traditional emerging approaches detecting pests. Literature suggests that implementing advanced measures could decrease infestation rates up 70%, substantially yield losses bolstering security. Notably, adoption modern detection management techniques, (molecular diagnostics integrated pest management) geospatial surveillance analysis systems (spectral imaging, change-detection analysis) has shown greater effectiveness than methods. innovations offer promising avenues enhancing health securing chain against environmental shifts. The integration strategies crucial, demonstrating potential transform practices sustain productivity face changing climatic conditions. emphasizes importance adopting protect ensure resilience, providing valuable insights stakeholders across sector.

Language: Английский

Citations

9
Development of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues DOI Creative Commons
Arunee Suwanngam,

Philipp H. Schiffer,

Anongnuch Sasnarukkit

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 2, 2025

Language: Английский

Citations

1
Revolutionizing nematode management to achieve global food security goals - An overview DOI Creative Commons
Amir Afzal, Tariq Mukhtar

Heliyon, Journal Year: 2024, Volume and Issue: 10(3), P. e25325 - e25325

Published: Feb. 1, 2024

Nematodes are soil-dwelling organisms that inflict substantial damage to crops, resulting in significant declines agricultural productivity. Consequently, they recognized as one of the primary contributors global crop damage, with profound implications for food security. Nematology research assumes a pivotal role tackling this issue and safeguarding The pursuit nematology focused on mitigating nematode-induced promoting sustainable agriculture represents fundamental strategy enhancing Investment is crucial advance security objectives by identifying managing nematode species, developing novel technologies, comprehending ecology, strengthening capabilities researchers farmers. This endeavor constitutes an indispensable step toward addressing most pressing challenges achieving practices. Primarily, endeavors facilitate identification species responsible leading development effective management strategies. These strategies encompass utilization resistant varieties, implementation cultural practices, biological control, chemical interventions. Secondly, efforts contribute innovative technologies aimed at populations, such gene editing techniques confer resistance infestations crops. Additionally, exploration beneficial microbes, certain fungi bacteria, potential biocontrol agents against nematodes, holds promise. study ecology foundational domain fosters deeper comprehension biology ecological interactions. knowledge instrumental devising precise efficacious

Language: Английский

Citations

8
Detection Methods for Pine Wilt Disease: A Comprehensive Review DOI Creative Commons

Sana Tahir,

Sher Hassan,

Lu Yang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(20), P. 2876 - 2876

Published: Oct. 14, 2024

Pine wilt disease (PWD), caused by the nematode

Language: Английский

Citations

6
Editorial: Detection, characterization, and management of plant pathogens DOI Creative Commons
Islam Hamim, B. S. Sipes, Yanan Wang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 13, 2024

Citation: Hamim I, Sipes B and Wang Y (2024) Editorial: Detection, characterization, management of plant pathogens. Front. Plant Sci. 15:1354042. doi: 10.3389/fpls.2024.1354042

Language: Английский

Citations

5
Plant-parasitic nematode disease complexes as overlooked challenges to crop production DOI Creative Commons

Luisa Parrado,

Marisol Quintanilla

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: July 23, 2024

Plant diseases are caused by various microorganisms such as bacteria, fungi, viruses, and nematodes. These impact crop growth, reduce produce quality, lead to financial losses. disease can be single pathogens or interactions called “disease complexes”, involving two more pathogens. In these cases, the severity combined is greater than sum of each pathogen alone. complexes formed among plant-parasitic nematodes (PPNs) with occur. PPNs either enhance other incidence necessary for symptoms expressed. do so being wounding agents, vectors, modifiers plant biochemistry physiology, altering rhizosphere microbiome. This review identifies several PPNs-plant in production discuss how understanding key improving management practices.

Language: Английский

Citations

5
FTA-LAMP based biosensor for a rapid in-field detection of Globodera pallida—the pale potato cyst nematode DOI Creative Commons
Maria João Camacho, Débora C. Albuquerque, Maria L. Inácio

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 18, 2024

The combination of a sensitive and specific magnetoresistive sensing device with an easy DNA extraction method rapid isothermal amplification is presented here targeting the on-site detection Globodera pallida, potato endoparasitic nematode. FTA-cards were used for extraction, LAMP was developed nanoparticle functionalized magnetic-biosensor detection. combinatorial effect these three emerging technologies has capacity to detect G. pallida limit one juvenile, even when mixed other related species. This combined system far more interesting than what single technology can provide. Magnetic biosensors be any protocol forming new solution target pallida. probe designed in this study consistently distinguished (∆Vac binding/Vac sensor above 1%) from cyst nematodes below 1%). It confirmed that either extracted or Lab Kit enough quantity quality whenever present (alone samples), ensuring specificity sensitivity. work provides insights strategy construct advanced devices pathogens in-field diagnostics. runs separately but easily integrated into device.

Language: Английский

Citations

4
Do CRISPR-based disease diagnosis methods qualify as point-of-care diagnostics for plant diseases? DOI
Shamfin Hossain Kasfy, Farin Tasnova Hia, Tofazzal Islam

et al.

The Nucleus, Journal Year: 2024, Volume and Issue: 67(1), P. 11 - 24

Published: April 1, 2024

Language: Английский

Citations

4
Upgrading Strategies for Managing Nematode Pests on Profitable Crops DOI Creative Commons
Mahfouz M. M. Abd-Elgawad

Plants, Journal Year: 2024, Volume and Issue: 13(11), P. 1558 - 1558

Published: June 4, 2024

Plant-parasitic nematodes (PPNs) reduce the high profitability of many crops and degrade their quantitative qualitative yields globally. Traditional nematicides other nematode control methods are being used against PPNs. However, stakeholders searching for more sustainable effective alternatives with limited side effects on environment mankind to face increased food demand, unfavorable climate change, using unhealthy nematicides. This review focuses upgrading pre-procedures PPN as well novel measures durable management strategies economically important crops. Sound sampling, extraction, identification, counting PPNs related microorganisms, in addition perfecting designation nematode–host susceptibility/resistance, form bases these strategies. Therefore, frontiers should be expanded synthesize innovative integrated solutions The latter involve supplanting unsafe a new generation safe reliable chemical nematicidal bionematicidal alternatives. For better efficacy, materials techniques further developed via computer-aided nematicide design. Bioinformatics devices can reinforce potential biocontrol agents (BCAs) active components. They delineate interactions bionematicides targeted species tackle complex diseases. Also, functional plan based blueprint intended goals explored. Such currently engage succinate dehydrogenase, acetylcholinesterase, chitin deacetylase. Nonetheless, biochemical compounds targets earnestly sought. Commonly tested synergistic or additive function optimized sequential, dual-purpose, co-application agricultural inputs, especially pest schemes. Future directions research priorities address this novelty. Meanwhile, emerging bioactivated that offer reliability selectivity advanced favorable large-scale synthesis. Recent technological means intervene prevail over nematicide-related limitations. Nanoencapsulation challenge production costs, effectiveness, manufacturing defects some progress studying molecular plant–nematode interaction mechanisms exploited given topics such interfering RNA techniques, RNA-Seq BCA development, genome editing. A few recent materials/techniques agroecosystems decision support tools systems addressed. capability effectiveness operation harmony employing proper cooperative among all partners.

Language: Английский

Citations

4
Rapid Detection of Pratylenchus coffeae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks DOI
Caiyun Wu, Xueliang Xu,

Linjuan Fan

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102608 - 102608

Published: Feb. 1, 2025

Language: Английский

Citations

0