Escherichia coli Inoculation Decreases the Photosynthetic Performance on Tomato Plants: Clarifying the Impact of Human Commensal Bacteria on Transient Plant Hosts DOI Creative Commons
Anícia Gomes, Conceição Santos, Lia‐Tânia Dinis

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(7), P. 758 - 758

Published: July 17, 2024

The commensal/pathogenic Escherichia coli affects humans and animals, being present in diverse environmental niches, possibly surviving due to its adaptation transient plant hosts like crops, increasing the risk of foodborne diseases. E. interaction with host remains unknown, particularly impacts on photosynthesis. We hypothesize that influences tomato host’s photosynthetic capacity. To validate this hypothesis, we exposed 57-day-old plants (Solanum lycopersicum) different inoculation conditions, namely, non-inoculated (negative control, C−); directly injected SL6.1 (107 CFU/mL) (positive C+); irrigated one time CFU/mL); chronically (104 CFU/mL). No significant changes were observed chlorophyll fluorescence, pigments’ contents, morphological aspects, fruiting all conditions. However, (chronically one-time contaminated) had decreased stomatal conductance (gs, 31.07 34.42 mol m−2 s−1, respectively, vs. 53.43 48.08 s−1 C− C+, respectively), transpiration rate (E, 0.32 0.35 contaminated conditions 0.57 0.48 a trend increased intrinsic carboxylation (Ci, 384 361 ppm 321 313 respectively). inoculated presented more severe effects than remaining lower net (PN, 0.93 3.94–5.96 μmol (CO2) other conditions), water use efficiency (iWUE, 33.1 74.51–184.40 (CO2)/mmol (H2O) control plants), (iCE, 0.003 0.012–0.022 (CO2)/ppm conditions). Our data support some are similar those associated phytopathogenic bacteria. Lastly, propose decrease parameters gas exchange requires direct contact leaf/stomata, is mainly for high concentrations coli.

Language: Английский

Multiple Roles of Glycerate Kinase—From Photorespiration to Gluconeogenesis, C4 Metabolism, and Plant Immunity DOI Open Access
Leszek A. Kleczkowski, Abir U. Igamberdiev

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3258 - 3258

Published: March 13, 2024

Plant glycerate kinase (GK) was previously considered an exclusively chloroplastic enzyme of the glycolate pathway (photorespiration), and its sole predicted role to return most glycolate-derived carbon (as glycerate) Calvin cycle. However, recent discovery cytosolic GK revealed metabolic links for other processes. Although initially proposed as being solely regulated by substrate availability, subsequent discoveries redox regulation light involvement in production isoforms have indicated a more refined pathways conversion. Here, we re-evaluate importance emphasize multifaceted plants. Thus, can be major player several branches primary metabolism, including pathway, gluconeogenesis, glycolysis, C4 metabolism. In addition, recently, (but not cytosolic) isoform implicated part light-dependent plant immune response pathogen attack. The origins are also discussed here; it is produced cell compartments undergoes huge fluctuations depending on light/dark conditions. vacuolar transporter adds yet another layer our understanding transport/metabolism that two- three-carbon metabolites.

Language: Английский

Citations

6
Nucleoredoxin Vis-à-Vis a Novel Thioredoxin in Regulating Oxidative Stress in Plants: A Review DOI
Soham Hazra, Avishek Chatterjee, Somnath Bhattacharyya

et al.

Agricultural Research, Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Language: Английский

Citations

4
Alfalfa transcriptomic responses to the field pathobiome DOI Creative Commons
Lev G. Nemchinov, Brian M. Irish, Samuel Grinstead

et al.

Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract The pathobiome is a comprehensive biotic environment that includes community of all disease‐causing organisms within the plant, defining their mutual interactions and resultant effects on plant health. concept understanding alfalfa its impact host fitness in natural ecosystems remain largely unexplored. We have previously reported diverse composition field production environment. In this study, using modern transcriptomics tools combined with computational analyses, we applied novel ‘field genomics’ approach to survey gene expression changes visually healthy diseased plants collected from commercial fields. As result work, genes pathways involved responses were identified genetic basis crop's resistance multi‐pathogenic infections was proposed. addition offering insights into ecosystems, strategy can facilitate identification tolerant genotypes adapted pathobiome, followed by application breeding programs.

Language: Английский

Citations

0
Modulation of volatile emissions in olive trees: sustained effect of Trichoderma afroharzianum T22 on induced plant defenses after simulated herbivory DOI
Martin Aguirrebengoa, Beatriz Moreno, Rafael Alcalá Herrera

et al.

Biology and Fertility of Soils, Journal Year: 2024, Volume and Issue: 60(5), P. 593 - 602

Published: May 14, 2024

Language: Английский

Citations

3
The Impact of High-Light Stress on the Physiology and Transcriptome of Pterocladiella capillacea DOI
Hongyan Cai,

Z.R. Lv,

Jun Zeng

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109900 - 109900

Published: April 1, 2025

Language: Английский

Citations

0
Identification and characterization of the powdery mildew resistance in cultivated emmer wheat accession Lxd-682 via bulked segregant RNA sequencing DOI Creative Commons
Jiatong Li,

Ningning Yu,

Nina Sun

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 3, 2025

Common wheat (Triticum aestivum L.) is a vital source of nutrition for human consumption. However, production significantly threatened by various diseases, such as powdery mildew, widespread fungal disease caused Blumeria graminis f. sp. tritici (Bgt). Utilizing and identifying resistance genes elucidating the molecular mechanisms underlying this are most effective sustainable ways to fight disease. Lxd-682, cultivated emmer accession, exhibited 12 out 13 tested Bgt isolates at seedling stage. Genetic analysis revealed that conferred single dominant gene, tentatively designated PmLxd-682. Molecular mapping positioned PmLxd-682 between markers WGRE77413 WGRC1096, with Pm4-diagnostic marker JS717/JS718 co-segregating. Homology-based cloning sequence alignment further confirmed identical Pm4a. qRT-PCR showed alternative splicing PmLxd-682-V2 higher expression level than PmLxd-682-V1 post-Bgt invasion, suggesting its prominent role in fighting invasion. Additionally, four pathogenesis-related (PR) were up-regulated both Lxd-682 susceptible parent Langdon upon infection, revealing possibly unimportant roles pathway. Furthermore, 1,567 differentially expressed (DEGs) resistant bulks identified through BSR-Seq, 490 ones located within candidate interval on chromosome 2AL, potential biological processes associated enriched via gene ontology (GO) kyoto encyclopedia genomes (KEGG) pathway analysis. To verify regulatory genes, three key TRITD2 Av1G294940, Av1G036490 Av1G295220 all encoding protein, selected from six candidates following WGRC1096 be available marker-assisted selection (MAS) breeding practices. The study genetic intervals involved accession mildew. These findings advance our understanding plant-pathogen interactions establish solid foundation future functional studies aimed improving crops.

Language: Английский

Citations

0
Photorespiratory Metabolism and Its Regulatory Links to Plant Defence Against Pathogens DOI Open Access
Iwona Ciereszko, Elżbieta Kuźniak

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12134 - 12134

Published: Nov. 12, 2024

When plants face biotic stress, the induction of defence responses imposes a massive demand for carbon and energy resources, which could decrease reserves allocated towards growth. These growth–defence trade-offs have important implications plant fitness productivity influence outcome plant–pathogen interactions. Biotic stress strongly affects cells’ primary metabolism, including photosynthesis respiration, main source skeletons growth, development, defence. Although nature photosynthetic limitations imposed by pathogens is variable, infection often increases photorespiratory pressure, generating conditions that promote ribulose-1,5-bisphosphate oxygenation, leading to metabolic shift from assimilation photorespiration. Photorespiration, significant flux following photosynthesis, protects apparatus photoinhibition. However, recent studies reveal its role far beyond photoprotection. The intermediates cycle regulate photorespiration interacts with pathways nitrogen sulphur, shaping metabolism responses. This work aims present insights into integration within network under stress. It also explores potential regulating photosynthetic–photorespiratory against bacterial fungal pathogens.

Language: Английский

Citations

1
Part 2: aspects of the relation between photosynthesis and crop productivity DOI
Marko Iliev Kolaksazov

Botany Letters, Journal Year: 2024, Volume and Issue: 171(3), P. 267 - 280

Published: Jan. 11, 2024

Photosynthesis is amongst the basic physiological processes, affecting plants productivity. There can be different means to increase productivity, and most of them involve photosynthetic activity. influenced by many stressors, which exert effects, but they have in common a negative impact on Moreover, alleviation stress correspondingly decreases Thus, summarised effects were only generally mentioned this article, process photorespiration was underlined. Photorespiratory pathway relates photosynthesis also protect from oxidative at expense carboxylation. In addition, alterations disrupt balance between carbon nitrogen biomass plant. Being metabolic assimilatory pathways, such as biosynthesis proteins assimilaiton sulphur, binds One very important topic, especially under global CO2 content atmosphere relation carbohydrates protein biosynthesis. It affects differently C3 C4 species, thus specifics these reviewed well. are often considered more competitive productive, compared with other therefore preferred innovative crops future climatic conditions.

Language: Английский

Citations

1
Visualizing the dynamics of plant energy organelles DOI Creative Commons
Amanda M. Koenig, Bo Liu, Jianping Hu

et al.

Biochemical Society Transactions, Journal Year: 2023, Volume and Issue: 51(6), P. 2029 - 2040

Published: Nov. 17, 2023

Plant organelles predominantly rely on the actin cytoskeleton and myosin motors for long-distance trafficking, while using microtubules kinesin mostly short-range movement. The distribution motility of in plant cell are fundamentally important to robust growth defense. Chloroplasts, mitochondria, peroxisomes essential plants that function independently coordinately during energy metabolism other key metabolic processes. In response developmental environmental stimuli, these modulate their metabolism, morphology, abundance, meet need plant. Consistent with links processes like photorespiration fatty acid mobilization is frequently observed inter-organellar physical interaction, sometimes through organelle membranous protrusions. development various organelle-specific fluorescent protein tags has allowed simultaneous visualization movement living cells by confocal microscopy. These display an array morphology patterns redistribute within changes such as varying light conditions, temperature fluctuations, ROS-inducible treatments, pollen tube immune response, or association one another. Although there more reports mechanism chloroplast than our knowledge how why three move distribute still scarce at functional mechanistic level. It critical identify factors control coupled growth, development, stress response.

Language: Английский

Citations

3
Escherichia coli Inoculation Decreases the Photosynthetic Performance on Tomato Plants: Clarifying the Impact of Human Commensal Bacteria on Transient Plant Hosts DOI Creative Commons
Anícia Gomes, Conceição Santos, Lia‐Tânia Dinis

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(7), P. 758 - 758

Published: July 17, 2024

The commensal/pathogenic Escherichia coli affects humans and animals, being present in diverse environmental niches, possibly surviving due to its adaptation transient plant hosts like crops, increasing the risk of foodborne diseases. E. interaction with host remains unknown, particularly impacts on photosynthesis. We hypothesize that influences tomato host’s photosynthetic capacity. To validate this hypothesis, we exposed 57-day-old plants (Solanum lycopersicum) different inoculation conditions, namely, non-inoculated (negative control, C−); directly injected SL6.1 (107 CFU/mL) (positive C+); irrigated one time CFU/mL); chronically (104 CFU/mL). No significant changes were observed chlorophyll fluorescence, pigments’ contents, morphological aspects, fruiting all conditions. However, (chronically one-time contaminated) had decreased stomatal conductance (gs, 31.07 34.42 mol m−2 s−1, respectively, vs. 53.43 48.08 s−1 C− C+, respectively), transpiration rate (E, 0.32 0.35 contaminated conditions 0.57 0.48 a trend increased intrinsic carboxylation (Ci, 384 361 ppm 321 313 respectively). inoculated presented more severe effects than remaining lower net (PN, 0.93 3.94–5.96 μmol (CO2) other conditions), water use efficiency (iWUE, 33.1 74.51–184.40 (CO2)/mmol (H2O) control plants), (iCE, 0.003 0.012–0.022 (CO2)/ppm conditions). Our data support some are similar those associated phytopathogenic bacteria. Lastly, propose decrease parameters gas exchange requires direct contact leaf/stomata, is mainly for high concentrations coli.

Language: Английский

Citations

0