Phytoremediation: Harnessing plant power and innovative technologies for effective soil remediation
Malika Oubohssaine,
No information about this author
Ikram Dahmani
No information about this author
Plant Stress,
Journal Year:
2024,
Volume and Issue:
14, P. 100578 - 100578
Published: Aug. 27, 2024
Language: Английский
Just FIND‐IT: Harnessing the true power of induced mutagenesis
Plant Biotechnology Journal,
Journal Year:
2024,
Volume and Issue:
22(11), P. 3051 - 3053
Published: July 9, 2024
In
nature,
genetic
variation
occurs
in
every
population
and
results
the
evolution
of
a
diversity
new
properties,
some
which
promote
survival
species.
To
accelerate
nature's
based
on
diversity,
plant
breeders
may
induce
additional
mutations
to
raise
number
variations
increasing
chances
obtain
varieties
with
desired
traits
like
improved
nutritive
quality,
yields
resilience
biotic
abiotic
stress
factors.
Induced
mutagenesis
chemical
mutagens
is
considered
non-GM
has
been
used
barley
(Hordeum
vulgare)
for
decades
(Hansson
et
al.,
2024).
Reverse
techniques
including
TILLING
(Targeting
Local
Lesions
Genomes)
screening
methodology
more
recently
TILLING-by-sequencing
spinoffs
are
tools
identify
individual
variants
valuable
genomic
alterations.
However,
these
hampered
by
low
mutation
capacity.
PCR-based
technique
designed
detect
mismatched
single
nucleotides
target
gene.
2023,
Szarejko
her
research
group
Poland
published
thorough
overview
success
stories
within
last
20
years
(Szurman-Zubrzycka
2023)
description
populations
different
cultivars
landraces
obtained
following
(Figure
1a).
The
sizes
range
between
1372
9600
variants.
frequencies
individually
chosen
dose-dependent
(1/154–1/2500
Kbp).
When
multiplied
(#
individuals
×
per
individual),
total
present
ranges
10
100
million
This
sound
lot,
but
genome
size
around
4300
Mbp
(here,
RGT
Planet;
Jayakodi
2020),
less
than
2%
entire
mutated.
severely
reduces
possibility
find
populations.
FIND-IT
technology
approach
overriding
constraints.
was
Science
Advances
2022
1b)
(Knudsen
2022)
provides
an
agile
high-throughput
screen
unprecedented
large
chemically
induced
variant
combines
systematic
sample
pooling
splitting
high-sensitivity,
droplet
digital
PCR
(ddPCR)–based
genotyping
targeted
identification
at
single-nucleotide
resolution.
ddPCR
1000-fold
sensitive
conventional
PCR.
applicable
any
living
organism
that
can
be
grown
field
or
culture.
experimental
outlined
detail
Knudsen
illustrated
schematically
Figure
1b.
total,
500
000
plants
today
available
screening.
were
also
developed
other
crops
microorganisms
using
sodium
azide
ethyl
methanesulphonate
(EMS)
as
mutagens.
Thus
key
sweet
seeds
white
lupin
(Mancinotti
2023),
eliminate
presence
anti-nutritional
saponins
quinoa
(Trinh
2024),
improve
phosphate
bioavailability
grain
(Madsen
avoid
hydroxynitrile
glucoside-derived
formation
pro-carcinogen
carbamate
whisky
production
S1;
Jørgensen
2024)
modify
flavour
profiles
Saccharomyces
species
use
industrial
brewing
(Stovisek
pipeline
knowledge
mutant
load
spectrum
doses
monitored
whole
sequencing
1b;
2022).
introduced
found
equally
distributed
over
seven
chromosomes
higher
observed
increased
mutagen
doses.
Thus,
libraries
generated
purposes:
Medium
(e.g.
1.7
mm
treatment
average
14
770
SNPs
plant)
gene-function
analyses
0.3
5565
breeding
whole-genome
documents
15%
transversions
85%
transitions
preference
C
>
T
G
A
1c).
Planet
genome,
1900
Mb
C's
G's.
Upon
dose,
randomly
positioned
each
8000
will
transitions.
Using
technology,
library
collection
350
now
analysed
containing
approximately
=
2
800
1b).
Because
only
harbours
nucleotides,
this
means
virtually
all
putative
sites
have
saturated.
saturated,
their
status
shifts
from
being
random
become
defined
distinct
one
collection.
Accordingly,
grains
carrying
specific
SNP
gene
identified
many
pools
TaqMan™
assay
You
know
there
just
FIND-IT.
Compared
existing
cereal
resources
relation
natural
pan-genome
accession
panel,
off-target
pressure
efficiently
reduced
backcrossing
parent
direct
crosses
elite
commercial
programmes.
context,
it
noticed
would
typically
adjusted
comparable
classical
step
two
parents
naturally
genomes
As
proof
principle,
we
demonstrated
efficiency
isolating
knockout
lines
dozen
amino
acid
exchanges
miRNA
promoter
S2;
Taking
advantage
fact
non-GMO
method,
data
directly
verified
growing
2022),
important
requirement
validate
crop
(Khaipho-Burch
2023).
current
development
high-quality
allows
strategies
highly
customized.
While
static
regarding
variant,
CRISPR
technologies
still
facing
multiple
challenges
field-applicable
(Cardi
stays
agile,
offers
high
flexibility
high-throughput,
compatible
today.
regularly
updated
lines,
customized
winter
versus
spring
libraries)
while
sensitivity
keeps
competitive.
isolated
line
load,
original
implemented
protocols
lose
during
yield
cycles
providing
fast
market
rollout
novel
traits.
We
thank
Carlsberg
Foundation
funding
support:
grant:
CF14-0461
(to
B.L.M.)
CF15-0236
B.S.).
authors
not
declared
conflict
interest.
Data
sharing
article
no
datasets
study.
S1.
Disentangling
glucoside
biosynthesis
metabolon
access
malting
barleys
free
production.
S2.
Barley
selected
agronomically
evaluated.
Please
note:
publisher
responsible
content
functionality
supporting
information
supplied
authors.
Any
queries
(other
missing
content)
should
directed
corresponding
author
article.
Language: Английский
Harnessing the Power of an Extensive EMS‐Induced Sorghum Population for Rapid Crop Improvement
Physiologia Plantarum,
Journal Year:
2024,
Volume and Issue:
176(4)
Published: July 1, 2024
Plant
breeders
leverage
mutagenesis
using
chemical,
biological,
and
physical
mutagens
to
create
novel
trait
variations.
Many
widely
used
sorghum
genotypes
have
a
narrow
genetic
base,
which
hinders
improvements
classical
breeding.
Enhancing
the
diversity
of
genome
thus
remains
key
priority
for
breeders.
To
accelerate
enhancement
sorghum,
an
extensive
library
comprised
seeds
from
150,000
individual
mutant
plants
Sorghum
bicolor
inbred
line
BTx623
was
established
ethyl
methanesulphonate
(EMS)
as
mutagen.
The
bulked
into
1498
pools
(~100
seed
heads
per
pool).
In
each
pool,
DNA
extracted
subset
screened
FIND-IT
technology
based
on
droplet
digital
PCR.
All
43
nucleotide
substitutions
that
were
identified,
demonstrating
potential
identify
any
EMS-derived
mutation
in
elite
within
days.
This
diverse
represents
largest
collection
mutants
ever
conceived,
estimated
cover
240%
all
possible
EMS-induced
points
genome.
Using
FIND-IT,
speed
at
specific
desired
can
be
identified
is
major
upgrade
conventional
reverse
techniques.
Additionally,
ease
valuable
variants
integrated
commercial
lines
far
simpler
less
expensive
process
compared
editing.
Genomic
variations
will
direct
utility
breeding
resource
applications,
allowing
enhanced
adaptation
climate
change
yield
marginal
environments.
Language: Английский
Enhancing coffee diversity: Insights into the impact of sodium azide mutagenesis on quantitative and qualitative traits in Coffea arabica L
Scientia Horticulturae,
Journal Year:
2024,
Volume and Issue:
330, P. 113043 - 113043
Published: March 6, 2024
Language: Английский
Recovery of Novel Sequence Variants in Chemically Mutagenized Seed and Vegetatively Propagated Coffea arabica L.
Horticulturae,
Journal Year:
2024,
Volume and Issue:
10(10), P. 1077 - 1077
Published: Oct. 9, 2024
The
negative
effects
of
climate
change
impact
both
farmers
and
consumers.
This
is
exemplified
in
coffee,
one
the
most
widely
consumed
beverages
world.
Yield
loss
high-quality
Coffea
arabica
L.,
due
to
spread
coffee
leaf
rust
(Hemileia
vastatrix),
results
lower
income
for
subsistence
volatile
prices
markets
cafes.
Genetic
improvement
crops
a
proven
approach
support
sustainable
production
while
mitigating
biotic
abiotic
stresses
simultaneously
maintaining
or
improving
quality.
However,
many
species,
including
hindered
by
low
genetic
diversity.
can
be
overcome
inducing
novel
variation
via
treatment
seeds
cells
with
mutagens.
To
evaluate
this
mutant
populations
created
incubating
seed
embryogenic
calli
chemical
mutagens
ethyl
methanesulphonate
sodium
azide
were
subject
reduced-representation
DNA
sequencing
using
ddRADseq
approach.
More
than
10,000
variants
recovered.
Functional
analysis
revealed
hundreds
sequence
changes
predicted
deleterious
gene
function.
We
discuss
challenges
unambiguously
assigning
these
as
being
caused
mutagenic
describe
purpose-built
computational
tools
facilitate
recovery
from
plant
populations.
Language: Английский
Phenotypic and genetic characterization of an Avena sativa L. germplasm collection of diverse origin: implications for food-oat breeding in Chile
Mónica Mathias-Ramwell,
No information about this author
Valentina Pavez,
No information about this author
Marco Meneses
No information about this author
et al.
Frontiers in Plant Science,
Journal Year:
2023,
Volume and Issue:
14
Published: Dec. 21, 2023
Oats
are
known
for
their
nutritional
value
and
also
beneficial
properties
on
human
health,
such
as
the
reduction
of
cholesterol
levels
risk
coronary
heart
disease;
they
an
important
export
product
Chile.
During
last
decade
(2010-2022)
over
90%
oat
cultivated
area
in
Chile
has
been
covered
with
Avena
sativa
L.
cv.
Supernova
INIA.
This
lack
genetic
diversity
a
context
climate
change
could
limit
long-term
possibility
growing
oats
The
present
study
is
phenotypic
analysis
132
cultivars
pure
lines
diverse
origin
that
can
be
considered
potential
breeding
material.
germplasm
was
evaluated
28
traits
analyzed
14
SSR
markers.
effects
genotypes
phenotype
were
significant
all
(
P
≤
0.05).
Most
exhibited
moderate
to
high
broad-sense
heritability
exceptions
yield
(H
2
=
0.27)
hulls
staining
0.32).
Significant
undesirable
correlations
between
generally
small
biological
importance,
which
auspicious
achieving
objectives.
Some
data
provided
here
have
not
previously
reported.
overall
(H’
0.68
±
0.18).
grouped
into
three
clusters,
differing
qualities
breeding.
Twenty-six
outperforming
INIA
identified
conventional
food-oats.
average
(He
0.58
0.03),
varying
0.32
(AM22)
0.77
(AME178).
Two
subpopulations
supported
by
Structure
algorithm
distance
0.24,
showing
low
divergence
germplasm.
values
found
this
collection
promising
respect
obtaining
gain
short
term
programs.
However,
similar
diversity,
higher
better
performance
created
compared
foreign
suggest
harboring
new
will
key
favor
quality
long
term.
Language: Английский
TILLCANN: A TILLING platform inCannabis sativafor mutation discovery and crop improvement
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 1, 2024
Abstract
Cultivation
of
Cannabis
sativa
is
increasing
because
its
therapeutic
value
and
recognition
as
a
multi-purpose
sustainable
crop.
Targeting
Induced
Local
Lesions
in
Genomes
(TILLING)
versatile
reverse
genetics
approach
that
unlocks
induced
variation
through
mutagenesis
to
accelerate
the
development
new
cultivars
contribute
functional
validation
genes.
Increasingly
efficient
next-generation
sequencing
technologies
genomic
resources
have
combined
make
TILLING
by
(TbyS)
an
attractive
technique
can
be
applied
cannabis.
Here
we
describe
protocol
for
C.
TILLCANN
platform
composed
1,633
M2
families.
As
demonstration
functionality
platform,
used
TbyS
perform
high
throughput
screening
novel
mutations
amplicons
genes
associated
with
important
agronomic
biochemical
traits
set
512
We
confirmed
14
identified
calculated
average
mutation
frequency
range
1/263
1/320
kb.
found
heterozygous
mutants
cannabis
homologue
Class
II
TEOSINTE
BRANCHED
1/CYCLOIDEA/PCF
(
TCP4
)
are
linked
alterations
leaf
number
morphology.
expect
genetic
variability
unlocked
performing
forward
or
screens
significantly
boost
breeding
programs
geared
toward
both
medicinal
industrial
hemp.
Gene
&
accession
numbers
Raw
data
generated
this
study
was
deposited
at
European
Nucleotide
Archive
xxx
xxx.
Genes
discussed
within
manuscript
correspond
cs10
genome
assembly
National
Center
Biotechnology
Information
GCA_900626175.2.
Language: Английский
Transitioning from the Green Revolution to the Gene Revolution: strengthening nutritional security using climate resilient traditional crops
Rushikesh Tahakik,
No information about this author
Amit G Deshmukh,
No information about this author
M. P. Moharil
No information about this author
et al.
Bulletin of the National Research Centre/Bulletin of the National Research Center,
Journal Year:
2024,
Volume and Issue:
48(1)
Published: Dec. 9, 2024
Abstract
Background
World
food
production
has
significantly
increased,
largely
attributed
to
advancements
in
breeding
methods
pioneered
previous
decades.
These
can
be
categorized
into
three
major
eras—the
pre-Green
Revolution
era,
the
Green
and
post-Green
era—which
we
call
Gene
Revolution.
The
era
was
characterized
by
utilization
of
mutation
forward
genetics
approaches.
Main
body
(1950s–1980s)
brought
about
improvements
crop
breeding,
irrigation,
fertilizer
use,
leading
a
substantial
rise
reduced
rural
poverty.
However,
focus
primarily
on
yield,
with
less
nutritional
quality.
After
Revolution,
wheat
rice
rapidly
increased
from
0.4–2
metric
tonnes
per
hectare
2–4
due
government
initiatives.
Nevertheless,
cultivation
other
crops,
including
indigenous
varieties
millet,
decreased.
In
while
advanced
genome
editing
technologies
such
as
CRISPR‒Cas9
TALENs
have
enhanced
content
climate
resilience
there
also
been
renewed
reviving
crops.
Conclusion
This
review
discusses
bring
these
orphan
nutria
crops
back
emphasizes
importance
passing
this
knowledge
future
generations,
ensuring
their
place
sustainable
agriculture
security.
Language: Английский