ZmHDT103 Negatively Regulates Drought Stress Tolerance in Maize Seedlings

Agronomy, Journal Year: 2024, Volume and Issue: 14(1), P. 134 - 134

Published: Jan. 4, 2024

Drought stress presents a significant threat to the growth and development of maize. It is important study genes mechanisms that contribute drought tolerance. In this study, we identified ZmHDT103 (that encodes histone deacetylase) by conducting homologous sequence comparison found expression in maize seedlings responsive treatment with polyethylene glycol (PEG). We utilized CRISPR/Cas9 gene editing technology generate three distinct knockout lines obtained ChinaMU mutant gene. Under conditions, mutants exhibited significantly lower water loss rate (WLR), relative electrolytic leakage (REL), hydrogen peroxide (H2O2) level, malonaldehyde (MDA) level than those their wild-type (WT) counterparts. Additionally, higher levels abscisic acid (ABA), content (RWC), peroxidase (POD), proline (Pro) WT control. These findings indicate acts as negative regulator tolerance

Language: Английский

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Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.)

BIO Web of Conferences, Journal Year: 2025, Volume and Issue: 158, P. 03021 - 03021

Published: Jan. 1, 2025

Several bacteria are found to be potential as antagonists due their capacity in producing extracellular enzymes amylase, protease, gelatinase, cellulose and chitinase. In this research, we collected from both healthy anthracnose-infecting chili plants (root, stem leaf). The results revealed that 27 bacterial isolates non-pathogenic non-hemolytic plants, which 10 isolated roots, 8 leaves, 7 stems 2 fruits. Morphology identification showed most of circular, irregular punctiform, with elevation flat, raised umbonate. White milky dominant, varied opacity (transparent, translucent opaque) textures (slimy, mucoid, shiny dry). Enzymatic activity test 11 produced amylase chitinase (isolates 11, 34, 42, 48, 111,118, 139, 188, 215, 216 218).

Language: Английский

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Biochemical Response of Maize Plants Grown in the Field Under Different Water Availability: Evaluating the Influence of Leaf Position and Growth Stage

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 629 - 629

Published: Feb. 28, 2025

Drought is a major abiotic stress factor in agriculture and could greatly affect the production of maize, which key food source. Understanding biochemical response maize throughout length plant its life cycle when exposed to water deficit an important step exploring new agricultural approaches minimizing crop losses. In this study, plants grown field were three different regimes (100%, 50%, 0% irrigation). The status top, middle, basal leaves was assessed at two stages their (vegetative reproductive) evaluate how respond deficits. results showed that, presence stress, development affected decreased. Antioxidant enzyme activity, oxidative damage, osmolyte levels influenced not only by irrigation but also section sample. Throughout cycle, lipid peroxidation, ascorbate peroxidase, starch increased all leaf sections. However, several responses are specific section: top increase protein carbonylation, superoxide dismutase, sugar levels; middle proline base dismutase cycle. These findings suggest that efforts minimize damage caused deficits must consider sections phases

Language: Английский

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Enhancing drought tolerance in cumin through physiological and genetic insights from a synthetic variety

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 24, 2025

Developing drought-tolerant crop varieties is essential for sustainable agriculture and food security, particularly in arid semi-arid regions. This study introduces a newly developed cumin variety (Cuminum cyminum L.), high-value medicinal plant, bred enhanced resilience against drought stress. The synthetic was evaluated over three growing seasons under normal irrigation (field capacity) severe stress (30% of field conditions. Results indicated that the exhibited 71.59% higher seed yield, 46.53% greater chlorophyll content, 24.03% more total chlorophyll, 52.90% increase proline accumulation compared to parental genotypes. Under conditions, enzymatic activity catalase, peroxidase, ascorbate peroxidase 131.81%, 122.05%, 264.78% higher, respectively, genotypes, contributing its improved tolerance. Furthermore, demonstrated 95.02% oil content favorable shifts composition Molecular analysis using ISSR markers revealed significant genetic diversity with polymorphic percentages ranging from 66.67 100%. Clustering principal coordinates (PCoA) confirmed clear distinctions between These findings highlight as promising candidate water-limited regions, combining tolerance superior agricultural productivity.

Language: Английский

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Anti-oxidant and Anticoagulant Effects of <i>Phyllanthus acidus</i> Leaves Extracts: An <i>In Vitro</i> and <i>In Vivo</i> Study

Journal of Natural Remedies, Journal Year: 2025, Volume and Issue: unknown, P. 667 - 680

Published: April 4, 2025

Background: Phyllanthus acidus is used traditionally in various diseases, analgesics, inflammation, rheumatism, bronchitis, asthma, respiratory disorders, hepatic diseases and diabetes Bangladesh, South India Southeast, Asian countries. The plant a good source of antioxidants. Aim: This study aims to investigate the vitro vivo anticoagulant activities an aqueous extract (Aq.) methanolic (MeOH) derived from P. leaves. Method: Ultrasonic-Assisted Extraction (UAE) method was solvents. ether (PE), chloroform (Chlo.), methanol (MeOH), (Aq.), phytochemical testing were done. All extracts processed for antioxidant test (DPPH FRAP), Total phenolic content, activated partial thromboplastin time, prothrombin time clot lysis activity. MeOH Aq. fractions assess in-vivo activity using clotting tail amputation model. Results: shows presence different phytoconstituents. Phenolic Content (TPC) found high (2.125 GAE mg/gm) (1.870 mg/gm). Clotlysis PE, Chlo., MeOH, respectively (300 (mg/ml)-49.05%,43.13%,53.84%, 66.00%). exhibited significant results Activated Partial Thromboplastin Time (APTT), Prothrombin (PT) studies. Conclusion: species (PA) has been historically utilised pathological conditions, possessing substantial traditional significance. polyphenolic compounds proteases within influence coagulation mechanism, as evidenced by present study, which evaluated both intrinsic extrinsic pathways, yielding statistically outcomes assessments. Major Findings: Significant qualities are displayed extracts, suggests that this may find use treatment cardiovascular diseases.

Language: Английский

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Genetic, molecular and physiological crosstalk during drought tolerance in maize (Zea mays): pathways to resilient agriculture

Planta, Journal Year: 2024, Volume and Issue: 260(4)

Published: Aug. 28, 2024

Language: Английский

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Regulation of Proline Accumulation and Protein Secretion in Sorghum under Combined Osmotic and Heat Stress

Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1874 - 1874

Published: July 6, 2024

Plants reprogramme their proteome to alter cellular metabolism for effective stress adaptation. Intracellular proteomic responses have been extensively studied, and the extracellular matrix stands as a key hub where peptide signals are generated/processed trigger critical adaptive signal transduction cascades inaugurated at cell surface. Therefore, it is important study plant understand its role in development response. This examined changes soluble sub-proteome of sorghum cultures exposed combination sorbitol-induced osmotic heat 40 °C. The combined significantly reduced metabolic activity altered protein secretion. While cells treated with alone had elevated proline content, osmoprotectant treatment remained unchanged, confirming that utilise processes distinct from those invoked by single stresses applied separately. Reactive oxygen species (ROS)-metabolising proteins proteases dominated differentially expressed identified subjected stress. ROS-generating peroxidases were suppressed, while ROS-degrading upregulated protection oxidative damage. Overall, our provides candidates could be used develop crops better suited an increasingly hot dry climate.

Language: Английский

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Potential role of apple wood biochar in mitigating mercury toxicity in corn (Zea mays L.)

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 267, P. 115619 - 115619

Published: Oct. 25, 2023

Mercury (Hg) is a very toxic decomposition-resistant metal that can cause plant toxicity through bioaccumulation and oxidative damage. Biochar, derived from organic waste agricultural garbage, an on-site modification technique improve soil health in heavy metals-polluted regions. The present experiment was designed to explore the role of apple biochar management mercury corn (Zea mays cv. 'PL535'). Different levels wood (0%, 2.5%, 5.0%, 7.5% w/w) along with different Hg concentrations (0, 20, 40, 60 mg/L) were used based on completely randomized design. Based results, HgCl

Language: Английский

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The role of plant proteases in the response of plants to abiotic stress factors

Frontiers in Plant Physiology, Journal Year: 2023, Volume and Issue: 1

Published: Dec. 18, 2023

The primary function of proteolytic enzymes is the hydrolysis peptide bonds. Enzymes responsible for catalyzing this reaction are commonly referred to as proteases. vast majority these belong class hydrolases and operate in aqueous environments. However, there a distinct group proteases known intramembrane proteases, which integral membrane proteins capable cleaving bonds hydrophobic environment biological membranes. Proteolysis serves several essential functions plant cells, ranging from degradation damaged unnecessary removal non-functional protein aggregates. It plays role quality control system even releases transcription factors proteins. Proteolytic processes indispensable at every stage development allow modification cell’s composition based on developmental needs environmental requirements. Proteases also enzymatic components cell that facilitate efficient regeneration many key metabolic pathways, such photosynthesis respiration. response cells entire organism various biotic abiotic stresses often requires remodeling complexes, or changes profile. By participating all processes, constitute crucial element cellular stresses. aim work review individual play stress factors, drought, salinity, cold, temperature, light.

Language: Английский

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Comprehensive characterization of protease inhibiting gene family, cis-regulatory elements, and protein interaction network in linseed and their expression upon bud fly infestation

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 2, 2024

Linseed, also known as flax is an important oilseed crop with many potential uses in paint, textile, food and pharmaceutical industries. Susceptibility to bud fly (Dasyneura lini Barnes) infestation a serious biotic concern leading severe yield penalty linseed. Protease inhibitors (PIs) are candidates that activate during the insect-pest attack modulate resistance. In present study, we explored PI linseed genome total of 100 LuPI genes were identified grouped into five distinct subgroups. The analysis cis-acting elements revealed almost all promoters contain several regulatory elementary related growth development, hormonal regulation stress responses. Across subfamilies PIs, specific domains consistently found conserved protein sequences. tissue-specific in-silico expression pattern via RNA-seq regulated different stress. through qRT-PCR 15 significant up-regulation LuPI-24, LuPI-40, LuPI-49, LuPI-53, LuPI-63 upon resistant genotype EC0099001 check variety Neela. This study establishes foundation resource for comprehending structural, functional, evolutionary dimensions protease

Language: Английский

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