The potential roles of acid invertase family in Dendrobium huoshanense: Identification, evolution, and expression analyses under abiotic stress

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127599 - 127599

Published: Oct. 21, 2023

Language: Английский

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Identification of the sweet orange (Citrus sinensis) bHLH gene family and the role of CsbHLH55 and CsbHLH87 in regulating salt stress

The Plant Genome, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 31, 2024

Abstract Salt stress is one of the primary environmental stresses limiting plant growth and production adversely affecting growth, development, yield, fruit quality Citrus sinensis . bHLH (basic helix‐loop‐helix) genes are involved in many bioregulatory processes plants, including phytohormone signaling, defense responses, biosynthesis specific metabolites. In this study, by bioinformatics methods, 120 CsbHLH were identified, phylogenetic analysis classified them into 18 subfamilies that unevenly distributed on nine chromosomes. The cis‐acting elements mainly hormone‐related elements. Seventeen exhibited significant differences expression under salt stress. Six with randomly selected for quantitative real‐time polymerase chain reaction (qRT‐PCR) validation. qRT‐PCR results showed a strong correlation transcriptome data. Phytohormones such as jasmonic acid (JA) essential biotic abiotic responses CsbHLH55 CsbHLH87 considered candidate target sweet orange MYC2 transcription factors JA signaling pathway. These main downstream effectors pathway can be activated to participate Activation inhibits reactive oxygen species improves tolerance plants. could breeding new transgenic salt‐resistant varieties orange.

Language: Английский

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Identification of Dof transcription factors in Dendrobium huoshanense and expression pattern under abiotic stresses

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: April 22, 2024

Introduction: DNA-binding with one finger (Dof) transcription factors (TFs) are a unique family of TFs found in higher plants that regulate plant responses to light, hormones, and abiotic stresses. The specific involvement Dof genes the response environmental stresses remains unknown D. huoshanense . Methods: A total 22 were identified from genome. Results: Chromosome location analysis showed DhDof distributed on 12 chromosomes, largest number located chromosome 8. phylogenetic tree revealed DhDofs could be categorized into 11 distinct subgroups. In addition common groups, DhDof4, DhDof5, DhDof17, AtDof1.4 ortholog clustered B3 subgroup. Group E was newly branch, among which DhDof6, DhDof7, DhDof8, DhDof9 an independent branch. conserved motifs gene structure differences motif composition DhDofs. dof domain near N-terminus highly contained C 2 -C -type zinc linked four cysteines. Microsynteny interspecies collinearity duplication events Large-scale had not occurred only pair 13. Synteny blocks more often between its relatives less Oryza sativa Arabidopsis thaliana Selection pressure indicated subject purifying selection. Expression profiles correlation analyses under hormone treatments several different expression patterns. DhDof20 DhDof21 highest levels co-expressed MeJA induction. cis -acting element each regulatory elements involved growth as well q RT-PCR demonstrated DhDof2 main ABA-responsive DhDof7 cold stress-related gene. IAA suppressed some candidates, SA inhibited most candidate genes. Discussion: Our results may provide new insights for further investigation screening core stress-resistance

Language: Английский

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Metabolic and Transcriptomic Profile Revealing the Differential Accumulating Mechanism in Different Parts of Dendrobium nobile

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5356 - 5356

Published: May 14, 2024

Dendrobium nobile is an important orchid plant that has been used as a traditional herb for many years. For the further pharmaceutical development of this resource, combined transcriptome and metabolome analysis was performed in different parts D. nobile. First, saccharides, organic acids, amino acids their derivatives, alkaloids were main substances identified Amino derivatives flavonoids accumulated strongly flowers; saccharides phenols flowers fruits; leaves nucleotide its leaves, flowers, fruits. Simultaneously, genes lipid metabolism, terpenoid biosynthesis, alkaloid biosynthesis highly expressed phenylpropanoids flavonoid roots; other metabolisms leaves. Furthermore, members metabolic enzyme families like cytochrome P450 4-coumarate-coA ligase showed differential effects on tissue-specific accumulation. Members transcription factor AP2-EREBP, bHLH, NAC, MADS, MYB participated widely ATP-binding cassette transporters some also positive These results systematically elucidated molecular mechanism accumulation enriched library specialized products promising candidate genes.

Language: Английский

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Genome-scale identification, expression and evolution analysis of B-box members in Dendrobium huoshanense

Heliyon, Journal Year: 2024, Volume and Issue: 10(12), P. e32773 - e32773

Published: June 1, 2024

B-box (BBX) proteins have been recognized as vital determinants in plant development, morphogenesis, and adaptive responses to a myriad of environmental stresses. These zinc-finger play pivotal role various biological processes. Their influence spans photomorphogenesis, the regulation flowering, imparting resilience wide array challenges, encompassing both biotic abiotic factors. Chromosome localization, gene structure conserved motifs, phylogenetic analysis, collinearity expression profiling, fluorescence quantitative tobacco transient transformation methods were used for functional localization pattern analysis DhBBX gene. A total 23 members identified from Dendrobium huoshanense. Subsequent evaluations effectively segregated these genes into five discrete evolutionary subsets. The predictions subcellular localizations revealed that all localized nucleus. genetic composition patterns showed majority consisted several exons, with few variations could be attributed transposon insertion. comprehensive using qRT-PCR was conducted unravel D. huoshanense, specific concentration on their hormone treatments cold stress. Subcellular reveals DhBBX21 DhBBX9 are located Our results provide deep comprehension complex regulatory mechanisms BBXs response hormonal stimuli. discoveries encourage further detailed focused investigations operational dynamics BBX family wider range species.

Language: Английский

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Underlying mechanism of Dendrobium huoshanense resistance to lead stress using the quantitative proteomics method

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 6, 2024

Lead affects photosynthesis and growth has serious toxic effects on plants. Here, the differential expressed proteins (DEPs) in D. huoshanense were investigated under different applications of lead acetate solutions. Using label-free quantitative proteomics methods, more than 12,000 peptides 2,449 identified. GO KEGG functional annotations show that these mainly participate carbohydrate metabolism, energy amino acid translation, protein folding, sorting, degradation, as well oxidation reduction processes. A total 636 DEPs identified, could induce expression most proteins. enrichment analysis suggested involved processes such homologous recombination, vitamin B6 flavonoid biosynthesis, cellular component organisation or biogenesis, biological regulation significantly enriched. Nearly 40 are DNA replication repair, RNA synthesis, transport, splicing. The effect stress may be achieved through photosynthesis, oxidative phosphorylation, production excess antioxidant substances. 9 photosynthesis-related 12 phosphorylation-related was up-regulated after stress. Furthermore, a 3 SOD, POD, CAT, 7 ascorbate-related metabolic enzymes Under stress, almost all key synthesis substances up-regulated, which facilitate scavenging oxygen-free radical scavenging. levels some sugar glycoside phenylpropanoid pathway, terpene pathway also increased. More 30 heavy metal transport Expression profiling revealed significant rise ABC-type multidrug resistance transporter, copper chaperone, P-type ATPase with exposure to Our findings lay basis for research response

Language: Английский

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Plant AT-rich protein and zinc-binding protein (PLATZ) family in Dendrobium huoshanense: identification, evolution and expression analysis

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 30, 2024

PLATZ (plant A/T-rich protein and zinc-binding protein) transcription factors are essential for plant growth, development, responses to abiotic stress. The regulatory role of genes in the environmental adaptation D. huoshanense is inadequately comprehended. genome-wide identification elucidates functions processes gene family. Our investigation encompassed examination structures chromosome distribution, construction phylogenetic tree with its relatives, analysis cis-acting elements expression profiles potentially implicated growth stress responses. Eleven DhPLATZs were classified into three clades (I, II, III) according their evolutionary homology. distribution these over six chromosomes indicated that both whole genome duplication (WGD) segmental events have contributed expansion this Ka/Ks revealed a pattern purifying selection after occurrences, suggesting little alterations functional divergence. collinearity microsynteny results DhPLATZ shared same conserved domains as paralogs from chrysotoxum. Expression profiling quantitative demonstrated had unique patterns response phytohormones cold Subcellular localization expressed nucleus, factors. These findings enhance our understanding genes' involvement species underscore significance important areas further research.

Language: Английский

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Genome-Wide Identification and Characterization of the bHLH Gene Family and Its Response to Abiotic Stresses in Carthamus tinctorius

Plants, Journal Year: 2023, Volume and Issue: 12(21), P. 3764 - 3764

Published: Nov. 3, 2023

The basic helix-loop-helix (bHLH) transcription factors possess DNA-binding and dimerization domains are involved in various biological physiological processes, such as growth development, the regulation of secondary metabolites, stress response. However, bHLH gene family C. tinctorius has not been investigated. In this study, we performed a genome-wide identification analysis tinctorius. A total 120 CtbHLH genes were identified, distributed across all 12 chromosomes, classified into 24 subfamilies based on their phylogenetic relationships. Moreover, subjected to comprehensive analyses, including protein sequence alignment, evolutionary assessment, motif prediction, promoter cis-acting elements. region revealed that encompass elements associated with aspects plant responses phytohormones, well both abiotic biotic stresses. Expression profiles, sourced from transcriptome databases, indicated distinct expression patterns among these genes, which appeared be either tissue-specific or specific certain cultivars. To further explore functionality, determined levels fifteen known harbor motifs related hormone responses. This investigation encompassed treatments ABA, salt, drought, MeJA. results demonstrated substantial variations response hormonal treatments. summary, our study establishes solid foundation for future inquiries roles regulatory mechanisms family.

Language: Английский

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