Genome-Wide Identification and Expression Analysis of the Sweet Cherry Whirly Gene Family DOI Creative Commons
Lili Wang, Qiandong Hou,

Guang Qiao

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(8), P. 8015 - 8030

Published: July 26, 2024

Sweet cherry (Prunus avium) is one of the economically valuable horticultural fruit trees and it widely cultivated throughout world. Whirly (WHY) genes are a unique gene family with few members have important biological functions in plant growth, development, response to abiotic stress. This study utilized whole-genome identification conduct comprehensive analysis WHY sweet examined their transcription levels different tissues under stress explore functions. Two were identified genome named PavWHY1 PavWHY2, respectively, based on homology those Arabidopsis thaliana. Both theoretical isoelectric points greater than seven hydrophilic proteins, suggesting that they may be localized plastids. The two evolutionarily classified into categories, large differences structure, highly similar protein tertiary structures, both conserved domains WHY. PavWHY2 collinear AtWHY1 AtWHY2, respectively. promoter sequence contains cis-acting elements related hormones stress, which differentially expressed during flower bud differentiation, cold accumulation. qRT–PCR showed development responded low temperature exogenous ABA treatment. recombinant plasmid pGreenII-0800-Luc promoters these can activate luciferase expression tobacco. Protein interaction predictions indicate products interact other proteins. reveals molecular features, evolutionary relationships, patterns genes, investigates activities promoters, lays foundation for further exploration provides new insights Rosaceae.

Language: Английский

WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants DOI Creative Commons
Wenfang Lin, Dongmei Huang,

Meng‐Si Li

et al.

Annals of Botany, Journal Year: 2024, Volume and Issue: 134(4), P. 521 - 536

Published: June 7, 2024

Abstract Plant senescence is an integrated programme of plant development that aims to remobilize nutrients and energy from senescing tissues developing organs under developmental stress-induced conditions. Upstream in the regulatory network, a small family single-stranded DNA/RNA-binding proteins known as WHIRLYs occupy central node, acting at multiple levels via trans-localization between nucleus organelles. In this review, we summarize current progress on role WHIRLY members senescence. can be traced back evolution green algae. trade off balance through maintaining organelle genome stability R-loop homeostasis, repressing transcription configuration condition, recruiting RNA impact editing splicing, evidenced several species. also act retrograde signal transducers organelles protein modification stromule or vesicle trafficking. addition, interact with hormones, reactive oxygen species environmental signals orchestrate cell fate age-dependent manner. Finally, prospects for further research promotion improve crop production constraints are highlighted.

Language: Английский

Citations

4
Functional Characterization of the PoWHY1 Gene from Platycladus orientalis and Its Role in Abiotic Stress Tolerance in Transgenic Arabidopsis thaliana DOI Creative Commons
Chun‐Quan Ou,

Zhiyu Dong,

Xudong Zheng

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 218 - 218

Published: Jan. 14, 2025

The frequent occurrence of extreme weather conditions in the world has brought many unfavorable factors to plant growth, causing growth and development plants be hindered even leading death, with abiotic stress hindering metabolism due severe uncontrollability. WHY1 transcription factor plays a critical role regulating gene expression plants, influencing chlorophyll biosynthesis, development, as well responses environmental stresses. important PoWHY1 adaptation become hot research topic. However, mechanism Platycladus orientalis under is still unclear. Here, was analyzed bioinformatically using P. study material, against Arabidopsis thaliana verified transgenic technology. It found that overexpression increased seed germination, decreased malondialdehyde accumulation, proline content, delayed senescence process salt stress. levels JAZ1, LOX1, ABI1, ABI2 were decreased, while RAB18, APX1, GSTF6, DREB2A increased, indicating enhanced tolerance A. thaliana. Furthermore, also drought From above results, it can concluded maintaining high leaves improve their adaptability. results provide scientific basis for understanding function lay foundation further on gene.

Language: Английский

Citations

0
Genome-wide identification and comprehensive analysis of the FtsH gene family in wheat DOI

Yuwei Li,

Hao Liu, Xiaoyu Wang

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: Feb. 3, 2025

Language: Английский

Citations

0
Genome-Wide Identification and Expression Analysis of the Sweet Cherry Whirly Gene Family DOI Creative Commons
Lili Wang, Qiandong Hou,

Guang Qiao

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(8), P. 8015 - 8030

Published: July 26, 2024

Sweet cherry (Prunus avium) is one of the economically valuable horticultural fruit trees and it widely cultivated throughout world. Whirly (WHY) genes are a unique gene family with few members have important biological functions in plant growth, development, response to abiotic stress. This study utilized whole-genome identification conduct comprehensive analysis WHY sweet examined their transcription levels different tissues under stress explore functions. Two were identified genome named PavWHY1 PavWHY2, respectively, based on homology those Arabidopsis thaliana. Both theoretical isoelectric points greater than seven hydrophilic proteins, suggesting that they may be localized plastids. The two evolutionarily classified into categories, large differences structure, highly similar protein tertiary structures, both conserved domains WHY. PavWHY2 collinear AtWHY1 AtWHY2, respectively. promoter sequence contains cis-acting elements related hormones stress, which differentially expressed during flower bud differentiation, cold accumulation. qRT–PCR showed development responded low temperature exogenous ABA treatment. recombinant plasmid pGreenII-0800-Luc promoters these can activate luciferase expression tobacco. Protein interaction predictions indicate products interact other proteins. reveals molecular features, evolutionary relationships, patterns genes, investigates activities promoters, lays foundation for further exploration provides new insights Rosaceae.

Language: Английский

Citations

0