GmARF15 Enhances the Resistance of Soybean to Phytophthora sojae by Promoting GmPT10d Expression in Response to Salicylic Acid Signalling DOI Open Access
Yunlong Huo, Haiyuan Chen, Zhuo Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 191 - 191

Published: Dec. 29, 2024

Phytophthora root and stem rot caused by sojae (P. sojae) is a globally prevalent oomycete disease. The use of resistant cultivars an effective environmentally friendly strategy to manage this It important understand the molecular mechanisms underlying response Glycine max (soybean) P. infection. In study, we demonstrated that isoflavonoid-specific prenyltransferase gene (GmPT10d, Glyma.10G070300) was significantly upregulated in soybean cultivar Williams 82 with high resistance Transgenic seedlings overexpressing GmPT10d exhibited enhanced sojae, those subjected RNA interference showed increased susceptibility pathogen. Yeast-one-hybrid electrophoretic mobility shift assays revealed GmARF15 could directly bind promoter GmPT10d. Further analysis function transgenic also sojae. Transactivation assay, luciferase qPCR promote expression indicated elevated salicylic acid levels were associated Taken together, these findings reveal regulatory mechanism which enhances potentially promoting through signaling pathway.

Language: Английский

Role of Plant Growth-Promoting Rhizobacteria in Sustainable Agriculture: Addressing Environmental and Biological Challenges DOI
Abdul Wahab, Farwa Batool, Gholamreza Abdi

et al.

Journal of Plant Physiology, Journal Year: 2025, Volume and Issue: 307, P. 154455 - 154455

Published: Feb. 20, 2025

Language: Английский

Citations

1
Phylogenetic relationship of WRKY transcription factors in Solanum and potato genes in response to hormonal and biotic stresses DOI Creative Commons
Dongyang Jiang, Wenjuan Huang,

Jia Liu

et al.

Plant Signaling & Behavior, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 11, 2025

WRKY transcription factors are important regulators of plant responses to environmental stresses and hormone signaling. This study analyzes the gene family in Solanum tuberosum by examining phylogenetic relationships, expression profiles, their roles abiotic stress responses. Phylogenetic tree was constructed using 322 genes from four species: S. tuberosum, pennellii, pimpinellifolium, lycopersicum. The results revealed conserved expanded across these species. We then studied 75 SotuWRKY response salt, drought, heat stresses, treatments (IAA, ABA, BABA, GA3, BAP). Results showed that 19, 25, 29 were regulated under respectively. Several (e.g. SotuWRKY03 SotuWRKY24) also biotic like Phytophthora infestans infection treatments, indicating involvement defense mechanisms. A co-expression network based on gene-to-gene correlations, where SotuWRKY52 identified as a hub gene, positively regulating six negatively four. These findings suggest potato play key signaling, potentially enhancing resistance diseases. provides new insights into other

Language: Английский

Citations

0
Modes of Action of Biocontrol Agents from Rhizosphere to Phyllosphere Signaling DOI
Bakhodir Mamarasulov, Kakhramon Davranov,

T. G. Gulyamova

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Wheat TaNADPO promotes spot blotch resistance DOI
Meng Yuan, Qingdong Zeng,

Lei Hua

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

ABSTRACT Bipolaris sorokiniana is a common soil-borne fungal pathogen that can infect various organs of wheat ( Triticum aestivum L.), causing diseases such as spot blotch, root rot, head blight, and black point. The genetic basis resistance to B. not yet fully understood. In this study, natural population 1,302 global germplasms was established inoculated with at the seedling stage in greenhouse. Resistance blotch assessed, revealing only about 3.8% exhibited moderate or higher levels. A genome-wide association study (GWAS) using high-density 660K single nucleotide polymorphism (SNP) data identified region on chromosome 1BL (621.2-674.0 Mb) 9 SNPs significantly associated p < 10e-4) resistance, designated Qsb.hebau-1BL . RNA sequencing qRT-PCR assays showed gene TraesCS1B02G410300 , encoding nicotinamide-adenine dinucleotide phosphate-binding oxidoreductase (TaNADPO), induced by Five SNP variations were found promoter TaNADPO lines without Transient expression Nicotiana benthamiana leaves cytoplasmic subcellular localization fusion protein green fluorescent (GFP) tag. Wheat transgenic overexpressing enhanced compared wildtype plants, accumulation reactive oxygen species (ROS). knockout EMS mutant turgidum NADPO tdnadpo-K2561 Gln125*) reduced lower ROS plants. summary, has been crucial for providing valuable insights developing blotch-resistant varieties through molecular breeding techniques.

Language: Английский

Citations

2
Overexpressing CsSABP2 enhances tolerance to Huanglongbing and citrus canker in C. sinensis DOI Creative Commons
Liting Dong, Shuang Chen,

Lanyue Shang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 8, 2024

Huanglongbing (HLB) and citrus canker, arising from Candidatus Liberibacter asiaticus ( Ca Las) Xanthomonas citri pv. Citri Xcc ), respectively, have been imposing tremendous losses to the global industry. Systemic acquired resistance (SAR) has shown be crucial for priming defense against pathogen in citrus. Salicylic acid (SA) binding protein 2 (SABP2), which is responsible converting methyl salicylate (MeSA) SA, essential full SAR establishment. Here, we characterized functions of four SABP2 genes CsSABP2-1 , V18A CsSABP2-2 CsSABP2-3 ) HLB canker. In vitro enzymatic assay revealed that all proteins had MeSA esterase activities, strongest activity. Their activities were inhibited by SA except . Four controlled a strong promoter 35S induced into Wanjincheng orange Citrus sinensis Osbeck) generate transgenic plants overexpressing CsSABP2 Overexpressing increased content action on SA. Resistance evaluation demonstrated only significantly enhanced tolerance HLB, although CsSABP2s The data suggested amino Val-18 active site plays key role function. Our study emphasized balancing levels regulating conferring broad-spectrum This finding offers valuable insights enhancing through engineering.

Language: Английский

Citations

1
Immunomodulating melatonin-decorated silica nanoparticles suppress bacterial wilt (Ralstonia solanacearum) in tomato (Solanum lycopersicum L.) through fine-tuning of oxidative signaling and rhizosphere bacterial community DOI Creative Commons
Munazza Ijaz,

Luqiong Lv,

Temoor Ahmed

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 12, 2024

Tomato (Solanum lycopersicum L.) production is severely threatened by bacterial wilt, caused the phytopathogenic bacterium Ralstonia solanacearum. Recently, nano-enabled strategies have shown tremendous potential in crop disease management. This study investigates efficacy of biogenic nanoformulations (BNFs), comprising silica nanoparticles (SiNPs) and melatonin (MT), controlling wilt tomato. SiNPs were synthesized using Zizania latifolia leaves extract. Further, MT containing BNFs through one-pot approach. Nanomaterials characterized standard characterization techniques. Greenhouse assays conducted to assess impact on tomato plant immunity resistance wilt. The exhibited a spherical morphology, with particle sizes ranging from 13.02 nm 22.33 for 17.63 21.79 BNFs, indicating relatively uniform size distribution consistent shape across both materials. experiments revealed that soil application outperformed SiNPs, significantly enhancing reducing incidence 78.29% plants maintaining oxidative stress homeostasis via increasing activities antioxidant enzymes such as superoxide dismutase (31.81%), peroxidase (32.9%), catalase (32.65%), ascorbate (47.37%) compared untreated infected plants. Additionally, induced salicylic acid activating defense-related genes (e.g., SlPAL1, SlICS1, SlNPR1, SlEDS, SlPD4, SlSARD1) involved phytohormones signaling High-throughput 16 S rRNA sequencing promoted growth beneficial rhizosphere bacteria (Gemmatimonadaceae, Ramlibacter, Microscillaceae, Anaerolineaceae, Chloroplast Phormidium) healthy diseased plants, while suppressing R. solanacearum abundance Overall, these findings suggest offer more promising sustainable approach managing

Language: Английский

Citations

1
DNA methylation in wheat: current understanding and future potential for enhancing biotic and abiotic stress tolerance DOI
Uzma Afreen, Kunal Mukhopadhyay, Manish Kumar

et al.

Physiology and Molecular Biology of Plants, Journal Year: 2024, Volume and Issue: 30(12), P. 1921 - 1933

Published: Dec. 1, 2024

Language: Английский

Citations

1
Dufulin Impacts Plant Defense Against Tomato Yellow Leaf Curl Virus Infecting Tomato DOI Creative Commons
Liping Huang, Yingying Tang, Shuaixin Wang

et al.

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 53 - 53

Published: Dec. 31, 2024

Tomato yellow leaf curl virus (TYLCV) poses a significant threat to tomato production, leading severe yield losses. The current control strategies primarily rely on the use of pesticides, which are often nonselective and costly. Therefore, there is an urgent need identify more environmentally friendly alternatives. Dufulin, novel compound that has been effective in controlling viral diseases tobacco rice, not yet tested against TYLCV. This study assessed efficacy dufulin TYLCV over three-year span from 2021 2023 through field trials, by monitoring disease symptoms titers. Additionally, this expression levels genes associated with systemic acquired resistance (SAR), specifically proteinase inhibitor II (PI II) non-expressor pathogenesis-related 1 (NPR1), using real-time qRT-PCR. chlorophyll nitrogen content leaves were also measured. Plants treated showed reduced symptomatology lower titers compared controls. Analysis gene revealed NPR1 was upregulated dufulin-treated plants, whereas PI consistently downregulated TYLCV-infected plants. Interestingly, increased healthy plants following seven-day post-treatment dufulin. Moreover, exhibited higher than controls, though no differences observed between water-treated Overall, application significantly bolstered plant’s defense response, effectively reducing enhancing resistance.

Language: Английский

Citations

1
Phosphatases: Decoding the Role of Mycorrhizal Fungi in Plant Disease Resistance DOI Open Access
Li Chen, Xiaoping Zhang, Qiang Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9491 - 9491

Published: Aug. 31, 2024

Mycorrhizal fungi, a category of fungi that form symbiotic relationships with plant roots, can participate in the induction disease resistance by secreting phosphatase enzymes. While extensive research exists on mechanisms which mycorrhizal induce resistance, specific contributions phosphatases to these processes require further elucidation. This article reviews spectrum fungi-induced and synthesizes current understanding how mediate effects, such as defense structures plants, negative regulation immune responses, limitation pathogen invasion spread. It explores role induced provides prospective future directions this field.

Language: Английский

Citations

0
Exploring common bean's defense arsenal: Genome-wide characterization of PR-1 gene family and its transcriptional response to Colletotrichum lindemuthianum inoculation DOI
Ana Luíza Trajano Mangueira de Melo, Mariele Porto Carneiro Leão, Manassés Daniel da Silva

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 174, P. 779 - 795

Published: Oct. 4, 2024

Language: Английский

Citations

0