A novel key virulence factor, FoSSP71, inhibits plant immunity and promotes pathogenesis in Fusarium oxysporum f. sp. cubense

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

ABSTRACT Fusarium wilt of banana ( Musa spp.), caused by oxysporum f. sp. cubense Foc ), poses a significant threat to the global industry. Particularly, tropical race 4 exhibits high pathogenicity toward major commercial cultivar Cavendish, and there are no effective control measures available. Here, we characterize novel effector protein, FoSSP71, from , which was significantly induced during early stages interaction could suppress BAX-triggered programmed cell death in Nicotiana benthamiana . Transient expression FoSSP71 N. leaves weaken upregulation genes involved SA signaling pathway flg22 reduce both reactive oxygen species bursts callose accumulation. To verify function deletion mutant created. The displayed reduced growth rate F. marked reduction virulence bananas compared wild type (WT). Furthermore, levels PR3 PR10 were downregulated infected with ΔFoSSP71 strain WT strain. These findings indicate that is essential for plays key role invasion. Therefore, presents potential target future control, offering scientific foundation breeding disease-resistant varieties developing measures. IMPORTANCE Effector proteins critical factors fungi, playing roles fungal infection plants. In this study, identified an important regulatory protein invasion 4). Understanding its mechanisms necessary. Our research indicates factor 4, as it suppresses plant immune responses inhibiting accumulation callose. lacking showed bananas, demonstrating controlling disease. provide basis resistant disease new strategies, crucial sustainable development

Language: Английский

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RNA INTERFERENCE-BASED GENE SILENCING OF DICER-LIKE 2 IN Fusarium oxysporum f. sp. cubense TROPICAL RACE 4 MITIGATES FUSARIUM WILT DISEASE IN BANANA

Biocatalysis and Agricultural Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 103541 - 103541

Published: Feb. 1, 2025

Language: Английский

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Current Progress in Microbial Biocontrol of Banana Fusarium Wilt: A Systematic Review

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 619 - 619

Published: Feb. 28, 2025

Fusarium oxysporum f. sp. cubense (Foc) poses a significant threat to global banana production. This systematic review updates current knowledge on the efficacy of various antagonistic microorganisms in controlling Foc, considering recent spread this disease new regions. The studies were systematically analyzed, focusing methodologies, results, and conclusions provide comprehensive overview research its practical implications. A total 118 reviewed, covering use such as Trichoderma spp., Bacillus Streptomyces Pseudomonas both pure cultures consortia. Most focused Foc TR4 Cavendish subgroup bananas originated from Asia. Microbial consortia demonstrated higher control percentage with lower variability, particularly genera Pseudomonas. In contrast, more commonly used for Streptomyces. choice between depends genus experimental context, each approach has distinct advantages. Although reviewed generally high quality, long-term is still lacking. Antagonistic represent promising alternative control, although their specific strain environmental conditions. It been observed that inoculating these onto seedlings before transplantation or combination organic matter enhances effectiveness. Localized testing formulation optimization are recommended improve application preventive suppressive tools soil against infections. highlights vast diversity microbial agents rates, modes action, additional benefits plant development beyond biocontrol. Furthermore, some achieved 100% at level under controlled These findings demonstrate biological viable integrated management. Future should prioritize approaches facilitate widespread adoption including formulation, field application, integration other methods.

Language: Английский

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Optimization of a CRISPR-Cas9 in vitro protocol for targeting the SIX9 gene of Fusarium oxysporum f.sp. cubense race 1 associated with banana Fusarium wilt

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 10, 2025

Introduction Fusarium wilt of bananas ( Musa spp.), a threat to sustainable banana production worldwide, necessitates immediate action control the disease. The current strategies are centered on preventing its spread or developing resistant varieties. However, very little is known about genetic machinery used by fungus infect and kill plants. Therefore, research should focused also in understanding plant-pathogen molecular interaction targeting virulent genes for knock-out Fusarium. This study aims standardize gene editing protocol using CRISPR Cas9 technology oxysporum f.sp. cubense race 1 (Foc1); specifically, induce targeted mutations particular effector gene, SIX9, Foc1. Methods An vitro was optimized protein target SIX9 testing two gRNAs, expression purification Cas9, included plasmids pHis-parallel1 pMJ922, E. coli BL21 Rosetta, independently. Results demonstrated that produced exhibits high enzymatic activity, comparable commercial standard. These findings underscore robustness in-house enzyme highlight suitability future biotechnological applications. Discussion facilitates recombinant enabling use various experimental settings accelerating editing, an area significant relevance today. will support studies banana-Fusarium identifying candidate disease resistance plant, lack virulence pathogen, establishing function SIX proteins evaluating fungus’s infection capacity through pathogenicity assays.

Language: Английский

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Histological Dissection of Fusarium-Banana Interaction Using GFP-Tagged Subtropical Race 4 Strain of Fusarium oxysporum f. sp. cubense on Banana Cultivars with Differing Levels of Resistance

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2472 - 2472

Published: Dec. 1, 2024

Fusarium wilt, caused by oxysporum f. sp. cubense (Foc), poses a significant threat to global banana production. This study used GFP-tagged subtropical race 4 strain of Foc (GFP-Foc-STR4) trace the pathogen's movement in different cultivars. These include resistant cultivar FHIA25 and Cavendish somaclone 'GCTCV119', as well susceptible cultivars including 'Lady Finger', 'FHIA02', 'Williams' Cavendish. GFP localization revealed that GFP-Foc-STR4 was able infect all tested cultivars, moving from roots rhizome aerial parts plant. Tyloses formation root vasculature, visualised with autofluorescence confirmed scanning electron microscopy, found restrict within xylem vessels, slowing its spread but not fully preventing infection. containment mechanism contributes host tolerance 'FHIA25' though it does confer complete immunity. The use fluorescently tagged provides valuable insight into infection process, supports efforts integrated management wilt banana.

Language: Английский

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