Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 26, 2023
Abstract
Background:
Osteomyelitis
is
a
severe
bone
marrow
infection,
whose
pathogenesis
not
fully
understood.
This
study
aims
to
explore
the
causal
relationship
between
immune
cell
characteristics
and
osteomyelitis,
in
hopes
of
providing
new
insights
for
prevention
treatment
osteomyelitis.
Methods:
Based
on
two
independent
samples,
this
employed
two-sample
Mendelian
randomization
(MR)
analysis
assess
731
(divided
into
7
groups)
Genetic
variants
were
used
as
proxies
risk
factors
ensure
selected
instrumental
variables
meet
three
key
assumptions
MR
analysis.
GWAS
data
came
from
public
catalog,
while
osteomyelitis
was
sourced
Finnish
database.
Results:
At
significance
level
0.05,
21
phenotypes
identified
having
with
development
In
B
group,
such
Memory
%B
cell,
CD20-
%lymphocyte
showed
positive
Naive-mature
IgD-
CD38-
AC
negative
relationship.
addition,
specific
cDC
Myeloid
TBNK
T
maturation
stage,
Treg
group
also
significant
associations
Through
reverse
analysis,
it
found
that
had
no
impact
these
phenotypes,
suggesting
occurrence
might
turn
affect
phenotypes.
Conclusion:
reveals
first
time
perspective
understanding
mechanism
These
findings
are
formulating
targeted
strategies,
hold
promise
improving
clinical
outcomes
patients’
Frontiers in Endocrinology,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 16, 2025
Mendelian
randomization
is
believed
to
attenuate
the
biases
inherent
in
observational
studies,
yet
a
meta-analysis
of
studies
osteoporosis
has
not
been
conducted
thus
far.
This
study
aims
evaluate
connection
between
potential
causal
factors
and
risk
by
synthesizing
evidence
from
studies.
The
databases
PubMed,
Web
Science,
Embase
were
systematically
searched
for
investigating
influencing
up
May
2024.
Meta-analyses
assess
associations
various
pathogenic
using
Randomization
quality
was
evaluated
according
Strengthening
Reporting
Observational
Studies
Epidemiology
via
(STROBE-MR)
guidelines.
A
total
706
potentially
relevant
articles
screened,
resulting
inclusion
53
systematic
review,
which
30
eligible
meta-analysis.
combined
findings
these
revealed
that
rheumatoid
arthritis,
inflammatory
bowel
disease,
sex
hormone
binding
globulin,
depression,
non-alcoholic
fatty
liver
primary
biliary
cholangitis
asthma
are
associated
with
increased
osteoporosis,
while
basal
metabolic
rate
gut
microbiota
(NB1n)
serves
as
protective
factor.
However,
association
obesity,
type
2
diabetes
mellitus,
metformin,
ulcerative
colitis,
leisure
sedentary
behaviors,
systemic
lupus
erythematosus,
serum
iron
found
be
nonsignificant.
In
summary,
our
indicates
significant
relationships
osteoporosis's
onset
progression
have
established
cholangitis,
rate,
(NB1n),
asthma.
https://www.crd.york.ac.uk/prospero/,
identifier
PROSPERO
CRD42024540504.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: May 21, 2024
Background
This
study
aimed
to
clarify
the
relationship
between
gut
microbiota
and
osteoporosis
combining
Mendelian
randomization
(MR)
analysis
with
animal
experiments.
Methods
We
conducted
an
on
differential
bacteria
using
open-access
genome-wide
association
(GWAS)
data
microbe
obtained
from
public
databases.
The
was
performed
two-sample
MR
analysis,
causal
examined
through
inverse
variance
weighting
(IVW),
Egger,
weighted
median,
mode
methods.
Bilateral
oophorectomy
employed
replicate
mouse
model,
which
assessed
by
micro
computed
tomography
(CT),
pathological
tests,
bone
transformation
indexes.
Additionally,
16S
rDNA
sequencing
fecal
samples,
while
SIgA
indexes
of
IL-6,
IL-1β,
TNF-α
inflammatory
factors
were
in
colon
samples.
Through
immunofluorescence
histopathology,
expression
levels
tight
junction
proteins,
such
as
claudin-1,
ZO-1,
occludin,
assessed,
conduct
correlation
related
environmental
performed.
Results
A
positive
observed
g_Ruminococcus1
risk
osteoporosis,
O_Burkholderiales
showed
a
negative
osteoporosis.
Furthermore,
there
no
evidence
heterogeneity
or
pleiotropy.
successful
replication
model
it
found
that
abundance
significantly
reduced,
g_Ruminococcus
increased
ovariectomized
(OVX)-mice.
intestinal
level
OVX
mice
decreased,
increased,
barrier
damage
occurred,
content
LPS
serum
increased.
is
strongly
positively
correlated
formation
factors,
indicators,
density,
volume
fraction,
trabecular
quantity,
whereas
negatively
resorption
shows
strong
factors.
Conclusion
may
regulate
development
microbiota-gut-bone
axis.
Frontiers in Genetics,
Journal Year:
2024,
Volume and Issue:
15
Published: April 8, 2024
Osteomyelitis
is
a
severe
bone
marrow
infection,
whose
pathogenesis
not
yet
fully
understood.
This
study
aims
to
explore
the
causal
relationship
between
immune
cell
characteristics
and
osteomyelitis,
hoping
provide
new
insights
for
prevention
treatment
of
osteomyelitis.
Based
on
two
independent
samples,
this
employed
two-sample
Mendelian
randomization
(MR)
analysis
assess
731
(divided
into
seven
groups)
Genetic
variants
were
used
as
proxies
risk
factors
ensure
that
selected
instrumental
variables
meet
three
key
assumptions
MR
analysis.
Genome-Wide
Association
Studies
(GWAS)
data
obtained
from
public
GWAS
catalog,
while
osteomyelitis
was
sourced
FinnGen.
At
significance
level
0.05,
21
phenotypes
identified
having
with
development.
In
B
group,
such
Memory
%
(percentage
memory
cells
within
total
population,
finger
ratio),
CD20-
%B
do
express
CD20
marker
their
surface),
lymphocyte
showed
positive
Naive-mature
IgD-CD38-absolute
counts
(AC)
negative
relationship.
addition,
specific
in
conventional
dendritic
(cDCs)
Myeloid
TBNK
(T
cells,
natural
killer
cells)
T
maturation
stage,
Treg
group
also
significant
associations
Through
reverse
analysis,
it
found
had
no
impact
these
phenotypes,
suggesting
occurrence
may
affect
phenotypes.
To
our
knowledge,
first
shed
light
development
thereby
providing
perspective
understand
mechanism
These
findings
are
formulating
targeted
strategies,
hold
promise
improve
outcomes
patients
Frontiers in Cardiovascular Medicine,
Journal Year:
2024,
Volume and Issue:
11
Published: April 25, 2024
Background
Multiple
observational
studies
have
shown
associations
between
thyroid
cancer
(TC)
and
cardiovascular
diseases
(CVDs).
However,
the
results
were
inconsistent,
potential
causal
genetic
relationship
remains
unclear.
Methods
The
instruments
of
TC
CVDs
derived
from
data
obtained
through
genome-wide
association
(GWAS).
We
performed
two-sample
Mendelian
randomization(MR)
methods
to
investigate
causality
on
CVDs.
Summary-level
statistics
for
CVDs,
including
heart
failure
(HF),
atrial
fibrillation
(AF),
coronary
artery
disease
(CAD),
myocardial
infarction
(MI),
ischemic
stroke
(IS)
venous
thromboembolism
(VTE).
primary
method
employed
in
this
MR
analysis
was
Inverse
Variance
Weighted
(IVW)
approach,
four
additional
algorithms
used:
MR-Egger,
weighted
median,
simple
mode,
mode.
Additionally,
we
assessed
reliability
pleiotropy,
heterogeneity
leave-one-out
sensitivity
analysis.
Results
In
analysis,
only
detected
genetically
predicted
HF
(IVW
method,
odds
ratio
(OR)
=
1.00134,
95%
confidence
interval
(CI):
1.00023–1.00244,
p
0.017).
There
no
with
CAD,
MI,
AF,
IS,
VTE.
Conclusion
Our
confirmed
HF.
It
is
crucial
closely
monitor
incidence
patients
give
comprehensive
clinical
intervention
based
conventional
treatment.
Brain and Behavior,
Journal Year:
2025,
Volume and Issue:
15(3)
Published: March 1, 2025
ABSTRACT
Background
The
exact
nature
of
the
link
between
breast
cancer
and
meningiomas
is
unknown,
although
observational
studies
have
shown
a
correlation
two.
Using
two‐sample
Mendelian
randomization
(MR)
strategy,
we
aimed
to
investigate
effect
on
meningiomas.
Methods
Three
sets
genetic
instruments
were
utilized
in
this
study
based
publicly
available
summary
data.
For
cancer,
selected
62
strongly
associated
SNPs;
separate
datasets
curated
for
HER2‐positive
HER2‐negative
subtypes.
MR
analyses
included
outlier
testing,
MR‐Egger
regression,
MR‐PRESSO,
weighted
median,
inverse
variance
approaches.
Results
analysis
demonstrated
significant
evidence
cancer's
meningioma
risk
(OR
=
1.213,
95%
CI
1.054–1.396,
p
0.007),
supported
by
1.456,
1.066–1.988,
0.021)
though
not
median
method
1.095,
0.914–1.311,
0.326).
Inverse
weighting
specifically
revealed
association
incidence
1.203,
1.048–1.381,
0.009).
Furthermore,
was
with
an
increased
malignant
1.64,
1.12–2.40,
0.011).
Conclusions
This
represents
first
investigating
causal
relationship
Our
findings
support
hypothesis
that
may
increase
risk.
Medicine,
Journal Year:
2025,
Volume and Issue:
104(21), P. e42437 - e42437
Published: May 23, 2025
Peripheral
atherosclerosis
(PAS),
characterized
by
lipid
plaque
accumulation
in
arterial
walls,
significantly
increases
cardiovascular
risk.
This
study
aimed
to
identify
molecular
biomarkers
and
elucidate
underlying
mechanisms
of
PAS
progression.
We
analyzed
2
gene
expression
omnibus
datasets
(GSE28829,
GSE100927)
differentially
expressed
genes
(
P
<
.05,
|log
FC|
≥
0.585).
Functional
enrichment
(Gene
Ontology/Kyoto
Encyclopedia
Genes
Genomes)
Mendelian
randomization
analyses
were
performed
using
genome-wide
association
quantitative
trait
loci
data.
Six
hub
validated
through
single-cell
RNA
sequencing
independent
datasets.
A
diagnostic
nomogram
was
developed
evaluated
calibration
curves,
decision
curve
analysis,
receiver
operating
characteristic
metrics.
Integrated
analysis
revealed
6
key
PAS-associated
(leukocyte
immunoglobulin-like
receptor
B1,
hematopoietic
cell-specific
lyn
substrate
1,
plasminogen
activator
urokinase,
C-type
lectin
domain
family
member
B,
phosphatidylinositol-4-phosphate
5-kinase
type
1
beta,
cofilin
2).
The
model
demonstrated
exceptional
accuracy,
achieving
area
under
the
curves
1.0
(training)
0.975
(validation).
confirmed
causal
relationships,
with
beta
showing
protective
effects
(odds
ratio
0.74–0.90),
while
leukocyte
urokinase
emerged
as
risk
factors.
multi-omics
identifies
novel
signatures
establishes
a
robust
tool.
findings
advance
our
understanding
pathogenesis
pave
way
for
personalized
therapeutic
strategies.
Aging,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 9, 2024
Background:
The
causal
relationship
between
gut
microbiota
and
peripheral
artery
disease
(PAD)
is
still
not
clear.
In
this
research,
we
employed
the
Mendelian
randomization
(MR)
technique
to
explore
potential
connection
211
species
PAD.
We
also
investigated
whether
effects
operate
in
both
directions.
Methods:
used
Genome-wide
Association
Studies
(GWAS)
summary
statistics
data
from
MiBioGen
FinnGen
consortia
conduct
a
two-sample
MR
analysis
link
Sensitivity
conducted
assess
robustness
of
results.
addition
that,
reverse
was
performed
examine
inverse
relationship.
Results:
variance
weighted
(IVW)
method
provided
evidence
supporting
9
specific
taxa
study
findings
indicated
that
family
Family
XI
(OR=1.11,
CI
1.00-1.24,
P=0.048),
genus
Lachnoclostridium
(OR=1.24,
1.02-1.50,
P=0.033),
Lachnospiraceae
UCG001
(OR=1.17,
1.01-1.35,
P=0.031)
are
risk
factors
associated
with
class
Actinobacteria
(OR=0.84,
0.72-0.99,
P=0.034),
Acidaminococcaceae
(OR=0.80,
0.66-0.98,
P=0.029),
Coprococcus2
(OR=0.79,
0.64-0.98,
Ruminococcaceae
UCG004
P=0.032),
UCG010
(OR=0.74,
0.58-0.96,
P=0.022),
order
NB1n
(OR=0.88,
0.79-0.98,
P=0.02)
may
be
Moreover,
our
did
uncover
any
PAD
nine
investigated.
Conclusions:
Our
research
has
confirmed
while
identifying
bacterial
communities
Medicine,
Journal Year:
2024,
Volume and Issue:
103(44), P. e40230 - e40230
Published: Nov. 1, 2024
Colorectal
cancer
(CRC)
is
a
significant
global
public
health
concern.
Several
observational
studies
have
examined
the
association
between
inflammatory
cytokines
and
risk
of
colorectal
cancer,
but
findings
been
inconsistent.
In
this
study,
we
employed
2-sample
Mendelian
randomization
(MR)
analysis,
primarily
using
inverse
variance
weighted
approach,
to
investigate
causal
relationship
CRC.
The
forward
MR
analysis
revealed
positive
higher
levels
interleukin
(IL)-16
(OR:
1.37,
P
=
.002),
vascular
endothelial
growth
factor
1.44,
.001),
MIG
1.23,
.040)
with
an
increased
rectal
cancer.
Conversely,
macrophage
colony-stimulating
0.80,
.010)
may
potentially
decrease
colon
reverse
it
was
found
that
linked
IL-1b
0.93,
.022),
IL-1ra
0.90,
IL-5
IL-9
.017),
TNF-a
0.91,
.003).
Additionally,
associated
elevated
FGF-Basic
1.10,
.028).
Consistent
results
were
also
in
MR-Egger,
median,
mode
analysis.
Our
study
presents
novel
evidence
supporting
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: March 13, 2024
The
association
between
psoriasis
vulgaris
and
bullous
pemphigoid
(BP)
remains
largely
unknown.
To
investigate
whether
there
is
a
causal
effect
BP.
Two-sample
bidirectional
Mendelian
randomization
(MR)
analyses
were
conducted
using
publicly
released
genome-wide
studies
(GWAS)
summary
statistics.
GWAS
statistics
for
BP
downloaded
online
from
FinnGen
Biobank
Documentation
of
the
R12
release,
which
includes
219
cases
218,066
controls.
data
extracted
Sakaue
et
al.,
comprises
5072
478,102
Single-nucleotide
polymorphisms
(SNPs)
associated
with
exposure
selected
as
instrumental
variables
by
performing
additional
quality
control
steps.
inverse-variance-weighted
(IVW)
method
was
used
primary
MR
analyses,
MR-Egger
regression,
weighted
mode
method,
median
simple
employed
sensitivity
analyses.
intercept
test
"leave-one-out"
analysis
performed
to
evaluate
horizontal
pleiotropy
potentially
influential
SNPs,
respectively.
Genetically
determined
log
odds
an
increased
risk
(IVW:
ratio
(OR)
=
1.263,
95%
confidence
interval
(CI):
1.013-1.575,
P=0.038).
Sensitivity
(OR=1.255,
95%CI:
0.973-1.618,
P=0.106),
Egger
(OR=1.315,
0.951-1.817,
P=0.126),
(OR=1.414,
0.823-2.429,
P=0.234)
(OR=1.177,
0.889-1.559,
P=0.254)
derived
directionally
consistent
relationship
genetically
predicted
risks
developing
On
contrary,
we
found
that
had
no
significant
on
OR=0.996,
P=
0.707),
indicating
unidirectionality
relationship.
tests
showed
evidence
pleiotropy.
No
SNP
driving
results
detected
leave-one-out
analysis.
Our
suggested
causally
increases
BP,
highlighting
need
potential
strategies
prevention
early
diagnosis
comorbid
in
patients
vulgaris.
Further
researches
into
this
underlying
mechanisms
are
warranted.
Frontiers in Oncology,
Journal Year:
2024,
Volume and Issue:
14
Published: March 21, 2024
Background
Although
observational
studies
suggest
a
correlation
between
psoriasis
(PS)
and
cancers,
it
is
still
unknown
whether
this
association
can
replace
causal
relationships
due
to
the
limitations
of
studies.
Therefore,
we
conducted
two-sample
Mendelian
randomization
(MR)
analysis
evaluate
relationship
PS
cancers.
Methods
genetic
summary
data
were
obtained
from
two
genome-wide
(GWAS).
We
employed
MR
Base
for
individuals
retrieving
tumors
distinct
locations.
Inverse-variance
weighted
was
principal
method
used
MR,
supplemented
by
median,
Egger,
simple
mode,
mode.
To
investigate
possible
link
performed
independent
meta-analysis
based
on
analyses.
Results
Two
analyses
both
found
no
significant
overall
cancers
(OR=1.0000,
95%
confidence
interval
[CI]:0.9999-1.0001,
P
=0.984;
OR=1.0000,
CI:0.9999-1.0001,
=0.761),
with
17
site-specific
In
analyses,
there
CI:
0.9999-1.0001,
=1.00,
I
2
=
0.0%),
Conclusions
Our
findings
do
not
support
More
population-based
experimental
investigations
will
be
required
better
understand
complicated
