Spectroscopy Journal,
Journal Year:
2023,
Volume and Issue:
1(1), P. 37 - 48
Published: April 24, 2023
The
loss
of
proteostasis,
which
results
in
the
accumulation
misfolded
proteins,
is
one
hallmarks
aging
and
frequently
associated
with
process.
Fibroblasts
are
a
cellular
model
widely
used
study
can
mimic
proteostasis
that
occurs
human
body.
When
studying
using
fibroblasts,
two
approaches
be
used:
fibroblasts
from
same
donor
aged
vitro
until
senescence
or
donors
different
ages.
A
previous
by
our
group
showed
first
approach
aging.
Thus,
this
work
aimed
to
spectroscopic
profile
dermal
four
ages
Fourier-transform
infrared
spectroscopy
identify
changes
protein
conformation
compare
those
obtained
study.
Partial
least
squares
regression
analysis
peak
intensity
suggested
older
were
characterized
an
increase
levels
antiparallel
β-sheets
decrease
intermolecular
β-sheets,
agreement
results.
Molecular Metabolism,
Journal Year:
2023,
Volume and Issue:
74, P. 101755 - 101755
Published: June 16, 2023
Recently,
the
hallmarks
of
aging
were
updated
to
include
dysbiosis,
disabled
macroautophagy,
and
chronic
inflammation.
In
particular,
low-grade
inflammation
during
aging,
without
overt
infection,
is
defined
as
"inflammaging,"
which
associated
with
increased
morbidity
mortality
in
population.
Emerging
evidence
suggests
a
bidirectional
cyclical
relationship
between
development
age-related
conditions,
such
cardiovascular
diseases,
neurodegeneration,
cancer,
frailty.
How
crosstalk
other
underlies
biological
mechanisms
disease
thus
particular
interest
current
geroscience
research.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(8), P. 1564 - 1564
Published: Aug. 4, 2023
Although
the
trigger
for
neurodegenerative
disease
process
is
unknown,
relevance
of
aging
stands
out
as
a
major
risk
development
neurodegeneration.
In
this
review,
we
highlighted
relationship
between
different
cellular
mechanisms
that
occur
consequence
and
transcription
factor
nuclear
erythroid-2-related
2
(NRF2)
connection
with
TAU
protein.
We
focused
on
NRF2
in
main
processes
involved
neurodegeneration
associated
aging,
such
genomic
instability,
protein
degradation
systems
(proteasomes/autophagy),
senescence,
stem
cell
exhaustion,
well
inflammation.
also
analyzed
effect
levels
its
aggregation
spread
process.
Finally,
investigated
interconnection
alterations
signaling
pathway
both
primary
secondary
tauopathies.
All
these
points
highlight
possible
therapeutic
target
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 14, 2025
Abstract
The
accumulation
of
intracellular
protein
aggregates
is
a
hallmark
aging.
In
hereditary
adult-onset
neuromuscular
diseases
(NMDs),
these
are
not
only
characteristic
but
also
pathogenic,
marking
age-related
disorders.
transition
from
age-associated
non-pathogenic
to
disease-driving
pathogenic
remains
poorly
understood.
Poly(A)
binding
nuclear
1
(PABPN1)
forms
in
post-mitotic
aged
cells.
However,
short
trinucleotide
expansion
PABPN1
leads
muscle
dysfunction
Oculopharyngeal
Muscular
Dystrophy
(OPMD),
where
insoluble
skeletal
become
defining
disease
feature.
Combining
an
array
advanced
imaging
modalities,
we
examined
the
morphological
differences
between
formed
by
and
variants.
Through
micro-
nanoscale
analyses,
identified
key
structural
aggregation
propensity
variants
both
differentiated
undifferentiated
cells
linked
mRNA
cellular
dysfunctions.
Our
findings
provide
new
insights
into
distinctions
their
implications
for
diseases.
Journal of Extracellular Biology,
Journal Year:
2025,
Volume and Issue:
4(1)
Published: Jan. 1, 2025
Abstract
Proteasomes
are
essential
for
protein
degradation
and
maintaining
cellular
balance,
yet
their
roles
in
extracellular
fluids
not
well
understood.
Our
study
investigates
the
freely
circulating
proteasome
blood,
to
uncover
its
unique
molecular
characteristics,
compared
intracellular
counterparts.
Using
a
transgenic
mouse
model,
mass
spectrometry,
biochemical
tools,
we
show
that
predominant
serum
is
free
uncapped
20S
particle,
which
seems
assemble
intracellularly
before
entering
bloodstream.
This
composed
of
constitutive
immuno
subunits
exhibits
all
three
catalytic
activities.
Moreover,
complex
displays
distinct
post‐translational
modifications,
indicating
specialization
roles,
as
demonstrated
by
enhanced
caspase‐like
activity.
We
also
found
physiological
stress
significantly
upregulates
levels,
paralleling
human
data.
research
highlights
specialized
characteristics
proteasomes,
offering
new
insights
into
turnover
blood
with
significant
implications
understanding
proteostasis
beyond
environment.
Detecting
tissue
components
is
valuable
in
histology.
Scanning
acoustic
microscopy
(SAM)
measures
the
attenuation
of
sound
(AOS)
through
sections
to
obtain
histological
images
without
need
for
staining.
AOS
values
are
reduced
as
tissues
break
down.
Here,
we
digested
specific
using
enzymes
and
followed
process
with
imaging
over
time.
Additionally,
applied
dyes
antibodies
inhibit
enzyme
activity
preserve
target
component
within
section.
Collagenase
bone
clearly
visualise
internal
structure.
The
showed
a
distinct
decline
values.
Actinase
cervical
artery
except
amyloid
deposits,
which
were
detected
by
Congo
red
Actinase-digested
lymphoid
cells
remained
horseradish
peroxidase
(HRP)-staining
positive.
Amylase
some
corpora
amylacea
(CA)
brain,
became
periodic
acid-Schiff
(PAS)
staining
negative
diminished
size
upon
electron
observation.
DNase
deleted
cell
nuclei,
those
stained
HRP
or
haematoxylin.
Residual
nuclear
matched
light
microscopy.
Specific
inhibition
preserved
materials.
Our
method
offers
practical
solution
intentionally
deleting
retaining
Furthermore,
it
provides
means
adjust
compare
degree
degradation
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(4), P. 547 - 547
Published: April 9, 2025
In
humans,
aging
is
an
inevitable
consequence
of
diminished
growth
processes
after
reaching
maturity.
The
high
order
biomolecules
in
cells
and
tissues
continuously
disturbed
by
numerous
physical
chemical
destructive
impacts.
Host-derived
oxidant-based
cytotoxic
agents
(reactive
species,
transition
free
metal
ions,
heme)
contribute
considerably
to
this
damage.
These
are
under
the
control
immediately
acting
antagonizing
principles,
which
important
ensure
cell
tissue
homeostasis.
review,
I
apply
concept
host-derived
their
interplay
with
principles
process.
During
aging,
energy
metabolism
supply
dioxygen
nutrients
increasingly
disturbed.
addition,
a
chronic
inflammatory
state
develops,
condition
known
as
inflammaging.
balance
between
protective
mechanisms
analyzed
depending
on
age-based
physiological
alterations
ATP
production.
Disturbances
associated
development
age-related
diseases
comorbidities.
An
enhanced
production
reactive
species
from
dysfunctional
mitochondria,
cellular
redox
homeostasis,
adaptations
hypoxia
highlighted.
Examples
how
disturbances
pathogenesis
persons
advanced
age
given.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 5, 2025
Abstract
Detecting
tissue
components
is
valuable
in
histology.
Scanning
acoustic
microscopy
(SAM)
measures
the
attenuation-of-sound
(AOS)
through
sections
to
obtain
histological
images
without
need
for
staining.
AOS
values
are
reduced
as
tissues
break
down.
Here,
we
digested
target
using
enzymes
and
followed
process
with
imaging
over
time.
Additionally,
applied
specific
dyes
antibodies
inhibit
enzyme
activity
preserve
component.
Collagenase
bone
clearly
visualise
internal
structure.
The
component
showed
a
distinct
decline
values.
Actinase
artery
except
amyloid
deposits,
which
were
detected
by
Congo
red
Actinase-digested
lymphoid
cells
remained
horseradish
peroxidase
(HRP)-staining
positive.
Amylase
some
corpora
amylacea
(CA)
brain,
became
periodic
acid-Schiff
(PAS)
staining
negative
diminished
size
upon
electron
observation.
DNase
deleted
cell
nuclei,
those
stained
HRP.
Residual
nuclear
of
matched
light
microscopy.
Specific
inhibition
preserved
materials.
Our
method
offers
practical
solution
intentionally
deleting
or
retaining
section.
Furthermore,
it
provides
means
adjust
compare
degree
degradation
198
words
Future Medicinal Chemistry,
Journal Year:
2023,
Volume and Issue:
15(10), P. 867 - 883
Published: May 1, 2023
Targeted
protein
degradation
(TPD)
aids
in
developing
novel
bifunctional
small-molecule
degraders
and
eliminates
proteins
of
interest.
The
TPD
approach
shows
promising
results
oncological,
neurogenerative,
cardiovascular
gynecological
drug
development.
We
provide
an
overview
technology
advancements
TPD,
including
molecular
glues,
proteolysis-targeting
chimeras
(PROTACs),
lysosome-targeting
chimeras,
antibody-based
PROTAC,
GlueBody
autophagy-targeting
chimera,
autophagosome-tethering
compound,
chimera
chaperone-mediated
autophagy-based
degraders.
Here
we
discuss
the
development
evolution
field,
variety
that
PROTACs
target
biological
repercussions
their
degradation.
particularly
highlight
recent
improvements
research
utilize
autophagy
or
endolysosomal
pathway,
which
enables
targeting
undruggable
targets.
