Infections by Alloscardovia omnicolens and Associated Clinical Conditions: A Systematic Review DOI Open Access
Antônio Neres Norberg, Paulo Roberto Blanco Moreira Norberg, Fernanda Castro Manhães

et al.

Journal of Advances in Medicine and Medical Research, Journal Year: 2023, Volume and Issue: 35(21), P. 284 - 293

Published: Sept. 27, 2023

Background and Aim: Alloscardovia omnicolens is a gram-positive, non-motile, non-sporulating bacillus that occasionally identified as member of the human microbiota. Despite its infrequent colonization humans, it has been detected in various anatomical sites, including gastrointestinal tract, urinary vaginal mucosa, oral cavity, potential to cause opportunistic infections. This systematic literature review aims analyze pathogenic A. underlying clinical conditions associated with infection. Methods: A scientific manuscripts was conducted using PubMed, SciELO, Google Scholar databases. The search terms employed included "Alloscardovia omnicolens," "infection" "culture," "identification". Manuscripts published English, Portuguese, Spanish, French from 2007 2023 were considered for review. Results: Following bibliographic selection process, total five articles discussing infections included, along an additional thirty-six analytical support. Conclusion: Infections caused by are predominantly medical conditions. detection this bacterium cultures organic specimens patients, particularly those neoplastic diseases, warrants attention when patients critical health states or exhibit risk factors Given antibiotic susceptibility profiles prevailing strains omnicolens, we recommend use narrow-spectrum antibiotics treatment these cases, exception Metronidazole. approach can effectively eliminate while minimally impacting broader microbiota composition.

Language: Английский

Novel technologies for the diagnosis of urinary tract infections DOI Creative Commons
Tomas A. Bermudez, Jonathan E. Schmitz,

Malcolm Boswell

et al.

Journal of Clinical Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

ABSTRACT Urinary tract infections (UTIs) impose a substantial burden on patient quality of life and urine testing accounts for the majority workload in many clinical microbiology laboratories. Traditional UTI diagnosis relies symptoms, urinalysis, culture which are interpreted based historical guidelines. This approach, while foundational, presents limitations, particularly complex cases. Low-level bacteriuria presence fastidious organisms often overlooked or entirely missed standard culture, stressing need novel diagnostic methods technologies. mini-review summarizes existing state diagnostics 2024 covers current upcoming technologies including rapid molecular-based pathogen identification, next-generation sequencing, advanced antimicrobial susceptibility testing. However, these represent unique challenges, as they implemented, will require field to adapt new concepts avoid misdiagnosis overtreatment.

Language: Английский

Citations

1
Pooled Antibiotic Susceptibility Testing for Polymicrobial UTI Performs Within CLSI Validation Standards DOI Creative Commons

Richard A. Festa,

F. R. Cockerill,

Rick L. Pesano

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(2), P. 143 - 143

Published: Feb. 1, 2025

Background/Objectives: Urinary tract infections (UTIs) pose an increasing risk of antimicrobial resistance, and novel diagnostic tests have been developed to address the limitations standard urine culture in these cases. It is important that be validated for agreement error rates against antibiotic susceptibility testing (AST) methods. Methods: Polymicrobial (≥two non-fastidious microorganisms) consecutive clinical specimens submitted UTI were included this analysis. Specimens tested with Pooled Antibiotic Susceptibility Testing (P-AST) broth microdilution/disk diffusion (BMD/DD) parallel. Performance characteristics, such as essential (EA%), very major errors (VMEs), (MEs), assessed using Clinical Laboratory Standards Institute (CLSI) standards. P-AST-resistant BMD/DD consensus-sensitive results heteroresistance. Real-world sample data used assess associations between organism counts average “sensitive” count per sample. Results: The P-AST isolate AST was ≥90%, VMEs <2.0%, MEs <3.0%, meeting CLSI guidelines verification validation studies. When heteroresistance accounted for, overall both <1.5%. presence additional organisms dropped number antibiotics from 9.8 one 2.5 five or more organisms. fastidious did not any meaningful impact. Conclusions: P-AST, a component Guidance® assay (Pathnostics, Irvine, CA, USA), performed within standards polymicrobial specimens.

Language: Английский

Citations

1
Improving Patient Outcomes While Reducing Empirical Treatment with Multiplex-Polymerase-Chain-Reaction/Pooled-Antibiotic-Susceptibility-Testing Assay for Complicated and Recurrent Urinary Tract Infections DOI Creative Commons
Emery Haley, Natalie Luke,

Howard Korman

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(19), P. 3060 - 3060

Published: Sept. 26, 2023

This study compared rates of empirical-therapy use and negative patient outcomes between complicated recurrent urinary tract infection (r/cUTI) cases diagnosed with a multiplex polymerase chain reaction or pooled antibiotic susceptibility testing (M-PCR/P-AST) vs. standard urine culture (SUC). Subjects were 577 symptomatic adults (n = 207 males n 370 females) presenting to urology/urogynecology clinics 03/30/2022 05/24/2023. Treatment recorded by the clinician surveys. The M-PCR/P-AST 252) SUC 146) arms after matching for confounding factors. chi-square Fisher's exact tests used analyze demographics clinical arms. Reduced empirical-treatment (28.7% 66.7%), lower composite events (34.5% 46.6%, p 0.018), fewer individual UTI-related medical provider visits hospitalization/an urgent care center/an emergency room (p < 0.05) observed in arm arm. A reduction UTI symptom recurrence patients ≥ 60 years old was 0.05). Study results indicate that test reduces empirical treatment r/cUTI cases.

Language: Английский

Citations

11
Microbroth dilution method for antibiotic susceptibility testing of fastidious and anaerobic bacteria of the urinary microbiome DOI Creative Commons

Wilson Geaman,

Brian I. Choi,

Jacob Kaindl

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(6)

Published: May 6, 2024

ABSTRACT Bacterial isolates from the human urinary microbiome have been extensively studied for their antibiotic resistance; however, little work has done on those that are difficult to grow in vitro . This study was designed qualify a serum-based medium, New York City Broth III (NYCIII), and broth microdilution method determine susceptibility of previously underreported or undescribed microbes time growing standard Mueller-Hinton broth. Here, we demonstrate NYCIII microbroth dilution can be an effective determination species found microbiome. We show this serves well characterize fastidious anaerobic no Clinical Laboratory Standards Institute (CLSI) guidelines, including several families Aerococcaceae , Lactobacillaceae Actinomycetaceae Previous studies using expanded quantitative urine culture reveal samples clinical patients commonly polymicrobial composition. Thus, test whether serve as viable harmonized capable supporting testing range fastidious, non-fastidious, microbes. propose methodology standardized comparable CLSI standards allow resistance uncharacterized bacteria. IMPORTANCE Antibiotic susceptibilities bacteria largely due difficulty them lab environment. The current Muller-Hinton broth, growth many these species, leaving microbiologists without method. To address need, offers survey high-throughput manner understudied with proposed NYCIII, which is both non-fastidious Broader standardization development antibiotic-resistant breakpoints

Language: Английский

Citations

2
Rapid and accurate testing for urinary tract infection: new clothes for the emperor DOI
Robert B. Moreland, Linda Brubaker,

Lana Tinawi

et al.

Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

SUMMARY Urinary tract infection (UTI) is among the most common infections in clinical practice. In some cases, if left untreated, it can lead to pyelonephritis and urosepsis. other UTI resolves without treatment. Clinical diagnosis typically based on patient symptoms and/or urinalysis, including urine dipsticks. The standard culture method sometimes employed identify suspected urinary pathogen (uropathogen) guide antimicrobial choice, but results are rarely available before 24 h. also misses fastidious, anaerobic, slow-growing uropathogens reports polymicrobial infections. unexplained combination of negative cultures with persistent distressing both patients clinicians. Given broad appreciation advantages provided by rapid testing (e.g., for COVID-19 or influenza A), a rapid, accurate diagnostic test needed deliver timely treatment seeking care that optimizes antibiotic stewardship. Herein, we discuss progress being made toward an accessible, (i.e., within hours), assay clinically useful treating clinician timeframe growth rate pathogen(s)). New emerging often overlooked current techniques reviewed.

Language: Английский

Citations

2
Macrolide Resistance in the Aerococcus urinae Complex: Implications for Integrative and Conjugative Elements DOI Creative Commons
Jyoti Lamichhane, Brian I. Choi, Natalie Stegman

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(5), P. 433 - 433

Published: May 10, 2024

The recognition of the Aerococcus urinae complex (AUC) as an emerging uropathogen has led to growing concerns due a limited understanding its disease spectrum and antibiotic resistance profiles. Here, we investigated prevalence macrolide within urinary AUC isolates, shedding light on potential genetic mechanisms. Phenotypic testing revealed high rate resistance: 45%, among total 189 isolates. Genomic analysis identified integrative conjugative elements (ICEs) carriers gene ermA, suggesting horizontal transfer mechanism resistance. Furthermore, comparison with publicly available genomes related pathogens ICE sequence homogeneity, highlighting for cross-species dissemination determinants. Understanding mechanisms is crucial developing effective surveillance strategies improving use. findings underscore importance considering broader ecological context dissemination, emphasizing need community-level combat spread microbiome.

Language: Английский

Citations

1
Schistosomiasis–Microbiota Interactions: A Systematic Review and Meta-Analysis DOI Creative Commons
Philip Afful,

Godwin Kwami Abotsi,

Czarina Owusua Adu-Gyamfi

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(10), P. 906 - 906

Published: Oct. 16, 2024

Schistosomiasis, a tropical disease affecting humans and animals, affected 251.4 million people in 2021.

Language: Английский

Citations

1
Urine Biomarkers Individually and as a Consensus Model Show High Sensitivity and Specificity for Detecting UTIs DOI Open Access

Marzieh Akhlaghpour,

Emery Haley, Laura Parnell

et al.

Published: July 3, 2023

We aimed to determine if infection-associated urine biomarkers can differentiate true urinary tract infection (UTI) from non-UTI controls. Midstream clean-catch samples were collected asymptomatic volunteers and symptomatic subjects &gt; 60 years old diagnosed with presumptive UTI in a specialty setting. Microbial identification density assessed using multiplex PCR/pooled antibiotic susceptibility test (M-PCR/P-AST) standard culture (SUC). Three (NGAL, IL-8, IL-1β) measured the same specimens. Definitive cases had positive microorganism detection by SUC M-PCR, while definitive regardless of microbial detection. observed strong correlation (R2 ≈ 1) between NGAL, IL-1β. Biomarker consensus criteria two or more sensitivity 90.2%, specificity 91.2%, predictive value (PPV) 91.7%, negative (NPV) 89.7%, accuracy 90.7%, likelihood ratio 10.28, 0.11 differentiating cases, density. IL-1β showed significant elevation microbe compared without identification. exhibited high distinguishing cases.

Language: Английский

Citations

3
Draft genome sequences of four Corynebacterium amycolatum strains isolated from female urine samples DOI Open Access

Alex Kula,

Grace Chilton,

James Damaso

et al.

Microbiology Resource Announcements, Journal Year: 2024, Volume and Issue: 13(9)

Published: July 31, 2024

ABSTRACT Corynebacterium amycolatum is an emerging pathogen of the urinary tract. Here, we present draft genomes for four strains isolated from urine collected symptomatic and asymptomatic female participants.

Language: Английский

Citations

0
Emerging pathogens Aerococcus urinae and Aerococcus sanguinicola from a Canadian tertiary care hospital DOI
Pramath Kakodkar,

Joel Scott,

Javera Tariq

et al.

Future Microbiology, Journal Year: 2024, Volume and Issue: 19(15), P. 1321 - 1332

Published: Aug. 7, 2024

and

Language: Английский

Citations

0