Impact of commercial gut health interventions on caecal metagenome and broiler performance

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 29, 2025

Abstract Background Maintaining gut health is a persistent and unresolved challenge in the poultry industry. Given critical role of chicken performance welfare, there pressing need to identify effective intervention (GHI) strategies ensure optimal outcomes farming. In this study, across three broiler production cycles, we compared metagenomes broilers provided with ionophores (as control group) against birds subjected five different GHI combinations involving vaccination, probiotics, prebiotics, essential oils, reduction ionophore use. Results Using binning strategy, 84 (≥ 75% completeness, ≤ 5% contamination) metagenome-assembled genomes (MAGs) from 118 caecal samples were recovered annotated for their metabolic potential. The majority these ( n = 52, 61%) had differential response all cohorts are associated parameter — European efficiency factor (EPEF). group exhibited highest EPEF, followed closely by cohort where probiotics used conjunction vaccination. use B, commercial Bacillus strain-based formulation, was determined contribute superior birds. supplementation generally affected abundance microbial enzymes relating carbohydrate protein digestion pathways energy, nucleotide synthesis, short-chain fatty acid drug-transport systems. These shifts hypothesised differentiate among groups highlighting beneficial several bacteria, including Rikenella microfusus UBA7160 species. Conclusions All GHIs shown be methods modulation, varying influences on MAG diversity, composition, functions. metagenomic insights greatly enhance our understanding microbiota-related pathways, enabling us devise enteric pathogens related products presenting new opportunities improve overall health.

Language: Английский

Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with multiple sclerosis

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 14, 2024

The microbial ecology of Mycobacterium avium subspecies paratuberculosis infections (MAP) within the context Multiple Sclerosis (MS) is largely an unexplored topic in literature. Thus, we have characterized compositional and predicted functional differences gut microbiome between MS patients with MAP (MAP+) without (MAP-) infection. This was done exposome (through self-reported filled questionnaires), principally anthropometric sociodemographic patterns to gain understanding dynamics. 16S rRNA profiling faecal samples (n = 69) performed for four groups, which differed by disease infection: healthy cohort (HC) MAP-; HC MAP+ ; . Using a dynamic strategy, infection time sampling as occupancy models, recovered core both individuals. Additional application neutral modeling suggests key genera that are under selection pressure hosts. These include members phyla Actinobacteriota, Bacteroidota, Firmicutes. As several subjects provided multiple samples, Quasi Conditional Association Test incorporates paired-nature found major Archaea. To consolidate treatment confounders, microbiome, outcome parameters, mediation analysis cohort. highlighted certain i.e., Sutterella, Akkermansia, Bacteriodes, Gastranaerophilales, Alistipes, Balutia, Faecalibacterium, Lachnospiraceae, Anaerostipes, Ruminococcaceae, Eggerthellaceae Clostridia-UCG-014 having mediatory effect using duration group. Our analyses indicate may be important target dietary lifestyle intervention

Language: Английский