The impact of pharmaceutical pollutants on daphnids – A metabolomic approach DOI Creative Commons
Katie O’Rourke, Christina Virgiliou, Georgios Theodoridis

et al.

Environmental Toxicology and Pharmacology, Journal Year: 2023, Volume and Issue: 100, P. 104157 - 104157

Published: May 22, 2023

Pharmaceuticals have been classified as emerging contaminants in the aquatic ecosystem, mainly due to their increased use and improper disposal. A significant range of pharmaceutical compounds metabolites globally detected surface waters pose detrimental effects non-target organisms. Monitoring water pollution relies on analytical approaches for detection, however, such are limited by sensitivity limit coverage wide compounds. This lack realism risk assessment is bypassed with effect-based methods, which complemented chemical screening impact modelling, able provide mechanistic insight pollution. Focusing freshwater this study we evaluated acute daphnids three distinct groups pharmaceuticals; antibiotics, estrogens, a commonly encountered environmentally relevant pollutants. Combining several endpoints mortality, biochemical (enzyme activities) holistic (metabolomics) discovered patterns biological responses. In study, changes enzymes metabolism e.g. phosphatases lipase, well detoxification enzyme, glutathione-S-transferase, were recorded following exposure selected pharmaceuticals. targeted analysis hydrophilic profile revealed up-regulation metformin, gabapentin, amoxicillin, trimethoprim β-estradiol. Whereas gemfibrozil, sulfamethoxazole oestrone resulted down-regulation majority metabolites.

Language: Английский

Toxicological effects assessment for wildlife in the 21st century: Review of current methods and recommendations for a path forward DOI Creative Commons
Thomas G. Bean,

Val R. Beasley,

Philippe Berny

et al.

Integrated Environmental Assessment and Management, Journal Year: 2023, Volume and Issue: 20(3), P. 699 - 724

Published: June 1, 2023

Model species (e.g., granivorous gamebirds, waterfowl, passerines, domesticated rodents) have been used for decades in guideline laboratory tests to generate survival, growth, and reproductive data prospective ecological risk assessments (ERAs) birds mammals, while officially adopted assessment schemes amphibians reptiles do not exist. There are recognized shortcomings of current vivo methods as well uncertainty around the extent which with different life histories terrestrial amphibians, reptiles, bats) than these commonly models protected by existing ERA frameworks. Approaches other validating additional animal testing being developed, but incorporation such new approach methodologies (NAMs) into frameworks will require robust validations against responses. This takes time, ability extrapolate findings from nonanimal studies organism- population-level effects wildlife remains weak. Failure adequately anticipate predict hazards could economic potentially even legal consequences regulators product registrants. In order be able use fewer animals or replace them altogether long term, vertebrate whole organism needed provide NAM validation short term. Therefore, it is worth investing resources potential updates standard test guidelines addressing need clear guidance on conduct field studies. Herein, we review improving advancing assessment, tools foreseeable future. Integr Environ Assess Manag 2024;20:699-724. © 2023 His Majesty King Right Canada The Authors. Integrated Environmental Assessment Management published Wiley Periodicals LLC behalf Society Toxicology & Chemistry (SETAC). Reproduced permission Minister Environment Climate Change Canada. article has contributed U.S. Government employees their work public domain USA.

Language: Английский

Citations

18
Potential for Machine Learning to Address Data Gaps in Human Toxicity and Ecotoxicity Characterization DOI Creative Commons

Kerstin von Borries,

Hanna Holmquist, Marissa B. Kosnik

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(46), P. 18259 - 18270

Published: Nov. 1, 2023

Machine Learning (ML) is increasingly applied to fill data gaps in assessments quantify impacts associated with chemical emissions and chemicals products. However, the systematic application of ML-based approaches still limited, their potential for addressing a wide range unknown. We prioritized chemical-related parameters toxicity characterization inform ML model development based on two criteria: (1) each parameter's relevance robustly characterize described by uncertainty results attributable parameter (2) predict values availability measured data. 13 out 38 developing approaches, while flagging another nine critical gaps. For all parameters, we performed space analysis assess further diverse considering structural diversity available data, showing that can potentially 8–46% marketed 1–10% Our systematically future efforts address characterization.

Language: Английский

Citations

18
Unlocking the Power of Transcriptomic Biomarkers in Qualitative and Quantitative Genotoxicity Assessment of Chemicals DOI Creative Commons
Anouck Thienpont, Eunnara Cho, Andrew Williams

et al.

Chemical Research in Toxicology, Journal Year: 2024, Volume and Issue: 37(3), P. 465 - 475

Published: Feb. 26, 2024

To modernize genotoxicity assessment and reduce reliance on experimental animals, new approach methodologies (NAMs) that provide human-relevant dose-response data are needed. Two transcriptomic biomarkers, GENOMARK TGx-DDI, have shown a high classification accuracy for genotoxicity. As these biomarkers were extracted from different training sets, we investigated whether combining the two in human-derived metabolically competent cell line (i.e., HepaRG) provides complementary information of genotoxic hazard identification potency ranking. First, applicability to TempO-Seq, high-throughput technology, was evaluated. HepaRG cells exposed 72 h increasing concentrations 10 chemicals eight known

Language: Английский

Citations

7
A new paradigm for regulatory sciences DOI Creative Commons
Gina M. Hilton,

Yadvinder Bhuller,

John E. Doe

et al.

Regulatory Toxicology and Pharmacology, Journal Year: 2023, Volume and Issue: 145, P. 105524 - 105524

Published: Nov. 2, 2023

Regulatory science, rooted in legal requirements, provides a mechanism for identifying, assessing, and managing harm to humans the environment from exposure hazardous substances. A challenge regulatory authorities is that many governing laws reflect scientific paradigm of mid-20th century. By nature legislative processes, most are not able readily adapt incorporate advances inherent an ever-evolving paradigm. Consequently, issue rigid frameworks has become prominent global discussions related incorporation reliable relevant modern technology fulfill needs. To explore this issue, we apply Thomas Kuhn's The Structure Scientific Revolutions as conceptual framework help understand natural progression paradigms (from normal anomaly, crisis, revolution, finally new normal), identify where now cycle, path towards revolution enables timely implementation best available science fulfil requirements.

Language: Английский

Citations

15
The impact of pharmaceutical pollutants on daphnids – A metabolomic approach DOI Creative Commons
Katie O’Rourke, Christina Virgiliou, Georgios Theodoridis

et al.

Environmental Toxicology and Pharmacology, Journal Year: 2023, Volume and Issue: 100, P. 104157 - 104157

Published: May 22, 2023

Pharmaceuticals have been classified as emerging contaminants in the aquatic ecosystem, mainly due to their increased use and improper disposal. A significant range of pharmaceutical compounds metabolites globally detected surface waters pose detrimental effects non-target organisms. Monitoring water pollution relies on analytical approaches for detection, however, such are limited by sensitivity limit coverage wide compounds. This lack realism risk assessment is bypassed with effect-based methods, which complemented chemical screening impact modelling, able provide mechanistic insight pollution. Focusing freshwater this study we evaluated acute daphnids three distinct groups pharmaceuticals; antibiotics, estrogens, a commonly encountered environmentally relevant pollutants. Combining several endpoints mortality, biochemical (enzyme activities) holistic (metabolomics) discovered patterns biological responses. In study, changes enzymes metabolism e.g. phosphatases lipase, well detoxification enzyme, glutathione-S-transferase, were recorded following exposure selected pharmaceuticals. targeted analysis hydrophilic profile revealed up-regulation metformin, gabapentin, amoxicillin, trimethoprim β-estradiol. Whereas gemfibrozil, sulfamethoxazole oestrone resulted down-regulation majority metabolites.

Language: Английский

Citations

14