Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records

Clinical Epigenetics, Journal Year: 2022, Volume and Issue: 14(1)

Published: April 27, 2022

Conflicting results regarding alterations to sperm DNA methylation in cases of spermatogenesis defects, male infertility and poor developmental outcomes have been reported humans. Bulls used for artificial insemination represent a relevant model this field, as the broad dissemination bull semen considerably alleviates confounding factors enables precise assessment fertility. This study was therefore designed assess potential predict fertility.A unique collection 100 samples constituted by pooling 2-5 ejaculates per from Montbéliarde bulls comparable ages, assessed fertile (n = 57) or subfertile 43) based on non-return rates 56 days after insemination. The profiles these were obtained using reduced representation bisulfite sequencing. After excluding putative sequence polymorphisms, 490 fertility-related differentially methylated cytosines (DMCs) identified, most which hypermethylated bulls. Interestingly, 46 genes targeted DMCs are involved embryonic fetal development, function maturation, related fertility genome-wide association studies; five further analyzed pyrosequencing. In order evaluate prognostic value DMCs, split between training 67) testing 33) sets. Using Random Forest approach, predictive built values set. accuracy 72% set individual collected an independent cohort 20 bulls.This study, conducted largest so far examined epigenetic analyses, demonstrated that methylome is valuable source biomarkers. next challenge combine with other data same improve quality better understand interplay molecular features regulation research may applications human medicine, where affects interaction female, thus making it difficult isolate factor.

Language: Английский

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Integrated multi-omics analyses reveals molecules governing sperm metabolism potentially influence bull fertility

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: June 23, 2022

Bull fertility is of paramount importance in bovine industry because semen from a single bull used to breed several thousands cows; however, so far, no reliable test available for prediction. In the present study, spermatozoa high- and low-fertility bulls were subjected high-throughput transcriptomic, proteomic metabolomic analysis. Using an integrated multi-omics approach molecular differences between identified. We identified total 18,068 transcripts, 5041 proteins 3704 metabolites spermatozoa, which expression 4766 785 33 dysregulated bulls. At transcript level, genes involved oxidative phosphorylation pathway found be downregulated, while at protein level metabolic pathways significantly downregulated that Taurine hypotaurine metabolism Integrated analysis revealed interaction major pathways, including Butanoate metabolism, Glycolysis gluconeogenesis, Methionine cysteine Phosphatidyl inositol phosphate, pyrimidine saturated fatty acid beta oxidation. These findings collectively indicate molecules governing sperm potentially influence fertility.

Language: Английский

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Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock

Animals, Journal Year: 2022, Volume and Issue: 12(21), P. 2955 - 2955

Published: Oct. 27, 2022

Nowadays, selection of superior male candidates in livestock as a source frozen semen based on sperm quality at the cellular level is not considered accurate enough for predicting potential fertility. Sperm transcriptome analysis approaches, such messenger RNA levels, have been shown to correlate with fertility rates. Using this technology growth has become principal method, which can be widely applied predict industry through transcriptome. It provides gene expression validate function spermatogenesis, fertilization, and embryo development, parameters This review proposes transcriptomic approach high-throughput method more accurately future.

Language: Английский

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PRM1 Gene Expression and Its Protein Abundance in Frozen-Thawed Spermatozoa as Potential Fertility Markers in Breeding Bulls

Veterinary Sciences, Journal Year: 2022, Volume and Issue: 9(3), P. 111 - 111

Published: March 3, 2022

Functional genes and proteins in sperm play an essential role bulls’ reproductive processes. They are more accurate determining bull fertility than conventional semen quality tests. Protamine-1 (PRM1) is a gene or protein crucial for packaging protecting DNA until fertilization affects normal function. This study analyzes the potential from PRM1 as markers different breeds of bulls utilized artificial insemination programs, expected to be tool interpreting Indonesia. used Limousin, Holstein, Ongole Grade divided into two groups based on fertility, high-fertility (HF) low (LF). The assessment included progressive motility (computer-assisted analysis), viability (eosin-nigrosine), plasma membrane integrity (HOS test). Sperm fragmentation (SDF) was assessed using acridine orange staining Halomax test. PRM deficiency evaluated with chromomycin A3 method. Moreover, expression measured qRT-PCR, abundance enzyme immunoassay Semen values, relative gene, quantity were significantly higher (p < 0.05) HF LF bulls. SDF values Additionally, at levels correlated 0.01) fertility. Therefore, candidate

Language: Английский

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Sperm long non-coding RNAs as markers for ram fertility

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: May 10, 2024

It is critical in sheep farming to accurately estimate ram fertility for maintaining reproductive effectiveness and production profitability. However, there currently a lack of reliable biomarkers semen quality fertility, which hindering advances animal science technology. The objective this study was uncover long non-coding RNAs (lncRNAs) sperm from rams with distinct phenotypes. Mature were allocated into two groups: high low (HF; n = 31; 94.5 ± 2.8%, LF; 25; 83.1 5.73%; P 0.028) according the pregnancy rates sired by (average rate; 89.4 7.2%). Total isolated highest- lowest-fertility ( 4, 99.2 1.6%, 73.6 4.4%, respectively) followed next-generation sequencing transcripts. We uncovered 11,209 lncRNAs HF LF. In comparison each other, 93 differentially expressed (DE) Of these, 141 mRNAs upregulated 134 downregulated between LF, respectively. Genes commonly enriched 9 + 2 motile cilium flagellum ABHD2, AK1, CABS1, ROPN1, SEPTIN2, SLIRP , TEKT3 . Moreover, CCDC39, CFAP97D1, SLIRP, TTC12 flagellated motility motility. Differentially top 16 KEGG pathways. Targets elucidate functions cis trans manner using genetic context lncRNA locus, sequences revealed 471 targets 10 lncRNAs. This illustrates existence potential that can be implemented analyzing determining used breeding soundness exams precision livestock ensure food security on global scale.

Language: Английский

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Genetic parameters and genome-wide association studies including the X chromosome for various reproduction and semen quality traits in Nellore cattle

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 10, 2025

Abstract Background The profitability of the beef industry is directly influenced by fertility rate and reproductive performance both males females, which can be improved through selective breeding. When performing genomic analyses, genetic markers located on X chromosome have been commonly ignored despite being one largest chromosomes in cattle genome. Therefore, primary objectives this study were to: (1) estimate variance components parameters for eighteen male five female traits Nellore including analyses; (2) perform genome-wide association studies functional analyses to better understand background cattle. Results percentage total direct heritability (h 2 ) explained x ranged from 3 32% (average: 16.4%) 9 67% 25.61%) traits, respectively. Among related breeding soundness evaluation, overall bull semen evaluation quality accounted highest proportion h relative with an average 39.5% 38.75%, number significant per trait 7 (seminal vesicle width) 43 (total major defects). zero five. A 683, 252, 694, 382, 61, 77 genes overlapped regions identified performance, quality, morphology, defects, bulls’ key candidate are PRR32 , STK26 TMSB4X TLR7 PRPS2 SMS SMARCA1 UTP14A BCORL1 . main gene ontology terms “Oocyte Meiosis”, “Progesterone Mediated Oocyte Maturation”, “Thermogenesis”, “Sperm Flagellum”, “Innate Immune Response”. Conclusions Our findings indicate role phenotypic variability reproduction Breeding programs aiming improve these should consider adding information their analyses.

Language: Английский

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Omics approaches to understand impact of heat stress on semen quality and fertility in bovines

Animal Reproduction Science, Journal Year: 2025, Volume and Issue: unknown, P. 107786 - 107786

Published: Jan. 1, 2025

Language: Английский

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Assessing Bovine Male Fertility in a Technological Age

Advances in anatomy, embryology and cell biology, Journal Year: 2025, Volume and Issue: unknown, P. 297 - 329

Published: Jan. 1, 2025

Language: Английский

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Proteomic fertility markers in ram sperm

Animal Reproduction Science, Journal Year: 2021, Volume and Issue: 235, P. 106882 - 106882

Published: Oct. 30, 2021

Language: Английский

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Significance and Relevance of Spermatozoal RNAs to Male Fertility in Livestock

Frontiers in Genetics, Journal Year: 2021, Volume and Issue: 12

Published: Dec. 9, 2021

Livestock production contributes to a significant part of the economy in developing countries. Although artificial insemination techniques brought substantial improvements reproductive efficiency, male infertility remains leading challenge livestock. Current strategies for diagnosis largely depend on evaluation semen parameters and fail diagnose idiopathic most cases. Recent evidences show that spermatozoa contains suit RNA population whose profile differs between fertile infertile males. Studies have also demonstrated crucial roles spermatozoal (spRNA) spermatogenesis, fertilization, early embryonic development. Thus, spRNA may serve as unique molecular signatures sperm play pivotal treatment fertility. This manuscript provides an update various populations, including protein-coding non-coding RNAs, livestock species their potential role quality, particularly motility, freezability, The contribution seminal plasma is discussed. Furthermore, we discussed significance rare RNAs (ncRNAs) such long ncRNAs (lncRNAs) circular (circRNAs) spermatogenic events.

Language: Английский

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