Exploring the effects of eugenol, menthol, and lidocaine as anesthetics on zebrafish glucose homeostasis DOI

Gabriela Maressa Machado Baesso,

Amanda Vitória Venâncio,

Lívia Costa Varela Barca

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2023, Volume and Issue: 276, P. 109784 - 109784

Published: Nov. 5, 2023

Language: Английский

Functional neuronal circuits emerge in the absence of developmental activity DOI Creative Commons
Dániel L. Barabási, Gregor F. P. Schuhknecht, Florian Engert

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 8, 2024

The complex neuronal circuitry of the brain develops from limited information contained in genome. After genetic code instructs birth neurons, emergence regions, and formation axon tracts, it is believed that temporally structured spiking activity shapes circuits for behavior. Here, we challenge learning-dominated assumption required circuit by quantifying its contribution to development visually-guided swimming larval zebrafish. We found visual experience had no effect on optomotor response (OMR) dark-reared then raised animals while pharmacologically silencing action potentials with sodium channel blocker tricaine. washout anesthetic, fish could swim performed 75-90% accuracy OMR paradigm. Brain-wide imaging confirmed came 'online' fully tuned, without requiring activity-dependent plasticity. Thus, sensory-guided behaviors can emerge through activity-independent developmental mechanisms.

Language: Английский

Citations

15
A zebrafish-based acoustic motor response (AMR) assay to evaluate chemical-induced developmental neurotoxicity DOI

Demetrius McAtee,

Ahmed Abdelmoneim

NeuroToxicology, Journal Year: 2024, Volume and Issue: 103, P. 60 - 70

Published: June 6, 2024

Language: Английский

Citations

5
Cryoanesthesia in tambaqui Colossoma macropomum: Behavioral and electrocardiographic responses DOI

Suzane Maia da Fonseca,

Clarissa Araújo da Paz,

Maria Klara Otake Hamoy

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 582, P. 740551 - 740551

Published: Jan. 8, 2024

Language: Английский

Citations

4
Per- and Polyfluoroalkyl Substances: Impacts on Morphology, Behavior and Lipid Levels in Zebrafish Embryos DOI Creative Commons

J J Albers,

John E. Mylroie,

Ashley Kimble

et al.

Toxics, Journal Year: 2024, Volume and Issue: 12(3), P. 192 - 192

Published: Feb. 29, 2024

The presence of per- and polyfluoroalkyl substances (PFASs) in aquatic environments is often persistent widespread. Understanding the potential adverse effects from this group chemicals on communities allows for better hazard characterization. This study examines impacts zebrafish (

Language: Английский

Citations

4
Etomidate as an anesthetic in Colossoma macropomum: Behavioral and electrophysiological data complement each other as a tool to assess anesthetic safety DOI Creative Commons
Thaysa de Sousa Reis, Daniella Bastos de Araújo,

Clarissa Araújo da Paz

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(8), P. e0305093 - e0305093

Published: Aug. 6, 2024

The use of anesthetic agents in the management fish farming or ornamental breeding aims to minimize stress and promote animal welfare. Therefore, this study investigate behavioral, electrocardiographic, ventilatory characteristics tambaquis exposed baths with etomidate. was conducted juvenile (27.38 ± 3.5g) n = 99, at etomidate concentrations 2–4 mg.L -1 , analyzing induction recovery behavior (experiment I), electrocardiogram II), opercular movement III). Fish high reached stage general anesthesia faster, however, time longer, characterizing a dose-dependent relationship. Cardiorespiratory analyzes demonstrated reduction heart rate (69.19%) respiratory (40.70%) depending on concentration used during induction. During period, there cardiorespiratory reversibility normality. proved be safe as an agent for species 2 3 short-term anesthesia, but higher doses animals showed slow gradual manner without excitability. hemodynamic effect due rapid decrease includes negative factor using Colossome macropomum anesthesia.

Language: Английский

Citations

4
Behavioral and neurophysiological effects of electrical stunning on zebrafish larvae DOI Creative Commons

D A Burkhardt,

Claire Leyden,

Carina Thomas

et al.

Lab Animal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Abstract Two methods dominate the way that zebrafish larvae are euthanized after experimental procedures: anesthetic overdose and rapid cooling. Although MS-222 is easy to apply, this takes about a minute act fish show aversive reactions interindividual differences, limiting its reliability. Rapid cooling kills several hours not listed as an approved method in relevant European Union directive. Electrical stunning promising alternative euthanasia for but has yet been fully established. Here we characterize both behavioral neurophysiological effects of electrical 4-day-old larvae. We identified electric field characteristics stimulus duration (50 V/cm alternating current 32 s) reliably euthanizes free-swimming agarose-embedded with easy-to-implement protocol. Behavioral analysis calcium neurophysiology lose consciousness stop responding touch visual stimuli very quickly (<1 s). Electrically stunned no longer coordinated brain activity. Their brains instead undergo series concerted whole-brain waves over course many minutes before cessation all signals. Consistent need implement 3R at stages animal experimentation, reliable achieved by potential refinement welfare more than 5 million used annually biomedical research worldwide.

Language: Английский

Citations

0
Neural-activity-regulated and glia-mediated control of brain lymphatic development DOI
Jia Li, Mingjian Liu, Wenjie Du

et al.

Cell, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
SG06, a Chalcone Derivative Targets PI3K/AKT1 Pathway for Neuroprotection and Cognitive Enhancement in an Alzheimer’s Disease-Like Zebrafish Model DOI

Santhanam Sanjai Dharshan,

R Meghana,

Stephen M. Rao

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

Language: Английский

Citations

0
High-resolution live imaging of tardigrade response to anoxia DOI

Anna-Mari Haapanen-Saaristo,

Sara Calhim, Ilkka Paatero

et al.

Micron, Journal Year: 2025, Volume and Issue: unknown, P. 103847 - 103847

Published: May 1, 2025

Language: Английский

Citations

0
Norepinephrine changes behavioral state through astroglial purinergic signaling DOI
Alex Chen, Marc Duque,

Altyn Rymbek

et al.

Science, Journal Year: 2025, Volume and Issue: 388(6748), P. 769 - 775

Published: May 15, 2025

Both neurons and glia communicate through diffusible neuromodulators; however, how neuron-glial interactions in such neuromodulatory networks influence circuit computation behavior is unclear. During futility-induced behavioral transitions the larval zebrafish, neuromodulator norepinephrine (NE) drives fast excitation delayed inhibition of activity. We found that astroglial purinergic signaling implements inhibitory arm this motif. In NE triggers release adenosine triphosphate (ATP), extracellular conversion ATP into adenosine, suppression activation hindbrain neuronal receptors. Our results suggest a computational role for an evolutionarily conserved axis NE-mediated brain state position astroglia as important effectors signaling.

Language: Английский

Citations

0