Integrated analysis of whole genome and transcriptome sequencing uncovers genetic differences between Zi goose and Xianghai flying goose DOI
Hongyu Ni, Yonghong Zhang, Yuwei Yang

et al.

Animal Genetics, Journal Year: 2023, Volume and Issue: 55(1), P. 147 - 151

Published: Dec. 12, 2023

Zi goose is a famous indigenous breed originating from northeast China with high annual egg production. Xianghai flying composite and bred by crosses of the wild swan goose. Our previous study revealed significant differences in muscle fiber characteristics between two populations. Here, we aimed to reveal underlying genetic basis above phenotype through whole-genome transcriptome analysis. A total 20 blood samples (10 geese 10 geese) were used for whole genome sequencing, eight breast tissue (four four RNA sequencing. Using F

Language: Английский

Effects of Salmonella Typhimurium infection on intestinal flora and intestinal tissue arachidonic acid metabolism in Wenchang chickens DOI Creative Commons

Shenghong Chen,

Yaochen Xie,

Dingqian Guo

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 24, 2025

Salmonella infections can lead to intestinal inflammation and metabolic disorders in birds. However, whether arachidonic acid (ARA) metabolism is involved -induced remains unclear. This experiment investigated the changes cecal flora ARA Hainan Wenchang chickens infected with S. Typhimurium using 16s rDNA sequencing targeted metabolomics. The results showed that levels of metabolites were increased cecum tissue after infection , including prostaglandin E2 (PGE 2 ), F2α (PGF 2α lipoxin A4 (LXA4), ± 8(9)-EET, 11(12)-EET, 8,9-DiHETrE. content key enzymes for production (Phospholipase A2 PLA2 Cyclooxygenase-2 COX-2) chicken tissues was infection. relative mRNA inflammatory factors also infection, Interferon-γ (IFN-γ), Transforming growth factor-β1 (TGF-β1), Interleukin-4 (IL-4), Interleukin-6 (IL-6). In HD11 cells, use a cyclooxygenase (COX) inhibitor reduced COX-2 PGF induced by effectively response. addition, number beneficial genera (e.g., Bifidobacterium Lactobacillus Odorobacterium ) significantly . present study revealed structure -infected chickens. this demonstrated activates pathway, which turn mediates development provide data support theoretical prevention control avian salmonellosis.

Language: Английский

Citations

1
Genome-wide association analysis identify candidate genes for feed efficiency and growth traits in Wenchang chickens DOI Creative Commons

Keqi Cai,

Ranran Liu,

Limin Wei

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: June 28, 2024

Abstract Background Wenchang chickens are one of the most popular local chicken breeds in Chinese industry. However, low feed efficiency is main shortcoming this breed. Therefore, there a need to find more precise breeding method improve chickens. In study, we explored important candidate genes and variants for growth traits through genome-wide association study (GWAS) analysis. Results Estimates genomic heritability traits, including residual intake (RFI) 0.05, average daily food (ADFI) 0.21, weight gain (ADG) 0.24, body (BW) at 87, 95, 104, 113 days age (BW87, BW95, BW104 BW113) ranged from 0.30 0.44. Important related were identified, such as PLCE1, LAP3, MED28, QDPR, LDB2 SEL1L3 genes. Conclusion The results identified provide theoretical basis development new molecular technology.

Language: Английский

Citations

7
MeRIP sequencing reveals the regulation of N6-methyladenosine in muscle development between hypertrophic and leaner broilers DOI Creative Commons
Zhijun Wang, Xing Ju, Kan Li

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(6), P. 103708 - 103708

Published: April 1, 2024

Meat production performance is the most important economic trait in broilers, and skeletal muscle, as largest organ animals, directly related to meat during embryonic postnatal growth development. N6-Methyladenosine (m6A) a chemical modification occurs on RNA adenosine that has been reported participate variety of biological processes all species. However, there are still few reports regulatory role muscle development poultry after birth. This study aims reveal distribution m6A sites chicken pectoralis major birth find out relationship between As representatives leaner (XH: Xinghua chicken) hypertrophic (WRR: White Recessive Rock significant differences body weight, fiber diameter, cross-sectional area XH WRR chickens. sequencing detected total 397 differentially expressed genes (DEGs) chicken, these DEGs were mainly enriched catalytic activity metabolic pathways. MeRIP results showed among 6,476 modified peaks, about 90% peaks (5,823) down regulated The joint analysis mRNA data found 145 with differential peak, ALKBH5 demethylase, was also included. highly expression tissue chickens suggest may play crucial regulating Our revealed rate, cross-section well methylation level metabolism level.

Language: Английский

Citations

6
The m6A Modification Regulates the Composition of Myofiber Types in Chicken Skeletal Muscle DOI Creative Commons

Yanju Shan,

Yifan Liu, Ming Zhang

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 104811 - 104811

Published: Jan. 1, 2025

Language: Английский

Citations

0
Transcriptome-wide study of mRNAs modified by m 6 A RNA methylation in the testis development of dairy goats DOI Creative Commons

Xinyang Ren,

Yingxin Qu,

Akang Shari

et al.

Animal Biotechnology, Journal Year: 2025, Volume and Issue: 36(1)

Published: April 30, 2025

N6-methyladenosine (m6A) is an important epigenetic modification in RNA, playing a crucial role regulating the production and aging of animal testicular sperm. This study extracted mRNA from tissue male goats before after sexual maturity, generating methylation map through preliminary experiments immunoprecipitation sequencing. The results showed that during development dairy goats, expression levels marker genes related to methylation-related enzymes changed significantly. A total 36,602 peaks 11,223 were identified two groups, including 2989 differential (427 upregulated 2562 downregulated) 1457 differentially expressed (833 624 downregulated). abundance m6A was positively correlated with gene levels. reports for first time profiles modifications across entire transcriptome Guanzhong providing new perspective genetic improvement goats.

Language: Английский

Citations

0
Dynamic Changes in the Gut Microbiota and Metabolites during the Growth of Hainan Wenchang Chickens DOI Creative Commons

Yingzhi He,

Jie Li,

Feifan Wang

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(3), P. 348 - 348

Published: Jan. 19, 2023

Gut microbiota and their metabolites play important roles in animal growth by influencing the host’s intake, storage, absorption, utilization of nutrients. In addition to environmental factors, mainly diet, chicken breed stage also affect changes gut microbiota. However, little research has been conducted on development its local chickens. this study, cecal different developmental stages Hainan Wenchang chickens (a native Bantam) were investigated using 16S rRNA sequencing untargeted metabolomics. With aging, structure tended be more stable. The relative proportions dominant bacteria phyla Firmicutes, Bacteroidetes, Proteobacteria showed with development. may have structural functional response nutrient metabolism immune requirements physiological states. Several microbial metabolic biomarkers statistical differences detected stages. that form networks significant related various stages, including uncultured_bacterium_f_Ruminococcaceae, Ruminococcaceae_UCG-014, Faecalibacterium, uncultured_bacterium_o_Bacteroidales, uncultured_bacterium_f_Lachnospiraceae. Partially differential significantly correlated short-chain fatty acids such as butyric acid. These findings provide new insights into molecular mechanisms breeds, well resources for biomarker identification improve efficiency.

Language: Английский

Citations

9
Exploring m6A methylation in skin Cancer: Insights into molecular mechanisms and treatment DOI
Mingjun Cai, Xueqing Li,

Xueyu Luan

et al.

Cellular Signalling, Journal Year: 2024, Volume and Issue: unknown, P. 111420 - 111420

Published: Sept. 1, 2024

Language: Английский

Citations

2
Regulatory role of N6-methyladenosine in intramuscular fat deposition in chicken DOI Creative Commons
Tong Zhang, Baojun Yu, Zhengyun Cai

et al.

Poultry Science, Journal Year: 2023, Volume and Issue: 102(10), P. 102972 - 102972

Published: July 26, 2023

Intramuscular fat (IMF) has a pivotal influence on meat quality, with its deposition being multifaceted physiological interaction of several regulatory factors. N6-methyladenosine (m6A), the preeminent epigenetic alteration among eukaryotic RNA modifications, holds crucial role in moderating post-transcriptional gene expression. However, there is dearth comprehensive understanding regarding functional machinery m6A modification context IMF poultry. Our current study entails an analysis disparities within breast and leg 180-day-old Jingyuan chickens. We implemented methylated immunoprecipitation sequencing (MeRIP-seq) (RNA-seq) to delve into distribution putative frameworks The findings demonstrated markedly higher content relative (P < 0.01). Furthermore, expression METTL14, WTAP, FTO, ALKBH5 was significantly diminished comparison that peaks primarily populated 3′untranslated regions (3′UTR) coding sequence (CDS) regions. leg, when juxtaposed breast, manifested 176 differentially genes (DMGs), including 151 hyper-methylated DMGs 25 hypo-methylated DMGs. Kyoto Encyclopedia Genes Genomes (KEGG) revealed pronounced enrichment biosynthesis amino acids, peroxisome, Fatty acid biosynthesis, fatty elongation, cell adhesion molecules (CAMs) pathways. Key DMGs, namely ECH1, BCAT1, CYP1B1 were implicated regulation muscle lipid anabolism. offers substantial insight forms robust foundation for further exploration mechanisms modulating deposition. This profound theoretical importance improving leveraging quality indigenous chicken breeds.

Language: Английский

Citations

6
Regulatory role of m6A epitranscriptomic modifications in normal development and congenital malformations during embryogenesis DOI Open Access
Yifan Yao, Peiqi Liu, Yue Li

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 173, P. 116171 - 116171

Published: Feb. 22, 2024

The discovery of N6-methyladenosine (m

Language: Английский

Citations

2
Transcriptome-Wide Study of mRNAs and lncRNAs Modified by m6A RNA Methylation in the Longissimus Dorsi Muscle Development of Cattle-Yak DOI Creative Commons

Chun Huang,

Rongfeng Dai,

Guangyao Meng

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(22), P. 3654 - 3654

Published: Nov. 17, 2022

Cattle-yak is a hybrid F1 generation of cattle and yak, which has history more than 3000 years shown better production performance higher economic benefits those yaks. However, up to now, there been no study on the transcriptome-wide m6A methylation profile bovine skeletal muscle its potential biological function during development. Here, we observed significant changes in expression levels muscle-related marker genes methylation-related enzymes development cattle-yak, overall content Longissimus dorsi 18-month-old cattle-yak decreased significantly. A total 36,602 peaks, 11,223 8388 lncRNAs were identified two groups, including 2989 differential peaks (427 up-regulated 2562 down-regulated peaks), 1457 differentially expressed (833 624 genes) 857 (293 564 lncRNAs). GO KEGG analysis revealed that they significantly enriched some pathways (Wnt signaling pathway MAPK pathway) high-altitude adaptation-related (HIF-1 pathway). Moreover, abundance was positively correlated with gene levels, while it negatively lncRNA levels. This indicates modification played an important role cattle-yak; however, regulation mechanism m6A-modified mRNA may be different. first report mRNAs atlas one will provide new perspectives for genetic improvement bovines.

Language: Английский

Citations

10