Effects of dietary Bopu powder supplementation on intestinal development and microbiota in broiler chickens

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 13, 2022

This study aimed to investigate the effect of dietary supplementation with Bopu powder on intestinal development and bacterial community composition in broiler chickens. A total 486 1-day-old arbor acres broilers were fed a basal diet (CON group), supplemented 50 mg/kg aureomycin (AB or 40 (BP group). The results showed that BP group had significantly lower serum tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, diamine oxidase concentrations higher IL-10 than CON (p < 0.05). Groups AB weight per unit length small intestine villus height 0.05), ratio crypt depth groups Compared group, increased transforming growth concentration mRNA expressions zonula occludens-1 (ZO-1) occludin, decreased mucosal TNF-α, IL-10, caspase-3, caspase-8 nuclear factor-kappa-B Bax/Bcl-2 mucosa Meanwhile, ZO-1, secretory immunoglobulin A, interferon-γ concentrations, glucose transporter type-2 sirtuin-1, IL-1β Dietary trefoil factor family member superoxide dismutase-1 bcl-2 associated X, reduced casepase-9 myeloid differentiation primary response-88 expression chickens relative Moreover, high-throughput sequencing microbial structure distinct from addition abundances Faecalibacterium Colidextribacter Therefore, our suggests synergic response microbiota powder, provides theoretical basis as potential substitute for antibiotics.

Language: Английский

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Effects of different addition levels of CHM-JM113 on growth performance, antioxidant capacity, organ index, and intestinal health of AA broilers

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: May 15, 2024

The purpose of the present study was to investigate effects different levels a Chinese herbal medicine formulation combined with JM113 (CHM-JM113) on growth performance, antioxidant capacity, organ index, and intestinal health AA broilers. broiler chicks were randomly allocated 5 treatments as follows: basic diet for control group, supplemented 0.25% CHM-JM113, 0.5% 1% CHM-JM113 2% treatment respectively. results showed that addition significantly reduced mortality ( p &lt; 0.01) improved European Broiler Index (EBI) 0.05), whereas it had no significance performance broilers &gt; 0.05). Comparing 0.5 group index liver, spleen bursa In terms morphology structure, increased VH VH/CD ratio, decreased CD in small intestine compared 1 additive dose being more effective probiotics natural antioxidants also content SOD serum 21-day-old 0.01), MDA 0.01). At 42 days age, organism accompanied by significant increase T-AOC CAT content. effect immunity, we found or better expression occludin claudin-1 segments For GATA-3, may have be used an antibiotic alternative production.

Language: Английский

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Dietary supplementation with Macleaya cordata extract alleviates intestinal injury in broiler chickens challenged with lipopolysaccharide by regulating gut microbiota and plasma metabolites

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 19, 2024

Introduction The prevention and mitigation of intestinal immune challenge is crucial for poultry production. This study investigated the effects dietary Macleaya cordata extract (MCE) supplementation on injury in broiler chickens challenged with lipopolysaccharide (LPS). Methods A total 256 one-day-old male Arbor Acres broilers were randomly divided into 4 treatment groups using a 2×2 factorial design 2 MCE supplemental levels (0 400 mg/kg) LPS 1 mg/kg body weight). experiment lasted 21 d. Results discussion results showed that increased average daily feed intake during days 0-14. have an interaction gain 15-21. significantly alleviated decreased induced by LPS. antioxidant capacity activity catalase reduced level malondialdehyde jejunal mucosa. addition elevated villus height ratio to crypt depth ileum. mRNA expression pro-inflammatory cytokines interleukin ( IL ) -6 IL-8 jejunum. mitigated LPS-induced up-expression factors IL-1β IL-17 abundance probiotic bacteria (such as Lactobacillus Blautia pathogenic Actinobacteriota , Peptostretococcaceae Rhodococcus ), leading alterations gut microbiota composition. altered several metabolic pathways such Amino acid metabolism, Nucleotide Energy Carbohydrate Lipid metabolism broilers. In these pathways, L-aspartic acid, L-Glutamate, L-serine, etc., phosphatidylcholine, phosphatidylethanolamine, thromboxane B2, 13-(S)-HODPE, etc. conclusion, effectively improved growth performance function LPS-challenged chickens, probably due modulation plasma metabolites.

Language: Английский

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Tannic Acid Extracted from Galla chinensis Supplementation in the Diet Improves Intestinal Development through Suppressing Inflammatory Responses via Blockage of NF-κB in Broiler Chickens

Animals, Journal Year: 2022, Volume and Issue: 12(18), P. 2397 - 2397

Published: Sept. 13, 2022

The objective of this study was to investigate the effects adding tannic acid (TA) extracted from

Language: Английский

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Effects of isoquinoline alkaloids as an alternative to antibiotic on oxidative stress, inflammatory status, and cecal microbiome of broilers under high stocking density

Poultry Science, Journal Year: 2024, Volume and Issue: 104(1), P. 104671 - 104671

Published: Dec. 13, 2024

This study investigated the effect of isoquinoline alkaloids as an alternative to bacitracin on growth performance, oxidative stress, inflammatory status, and ceca microbiome broilers raised under high stocking density (HSD). A total 1,500 one-day-old male Ross 308 chicks were randomly assigned five treatment groups, with 10 replicate pens per group 30 birds pen, for 37 days. The treatments included normal (NSD, birds/m²), HSD (15 50 ppm Bacitracin (BCT50), 80 (IQA80), 100 (IQA100). From days 11 24, had lower feed efficiency (P < 0.05) compared those in other treatments. heterophil-to-lymphocyte ratio malondialdehyde levels NSD IQA80 BCT50 0.05). higher IL-6 a villus height height-to-crypt depth groups Serum TNF-α was group. Alpha diversity not affected by treatments; however, beta showed reduced microbial diversity, prevalence Enterococcaceae Peptostreptococcaceae. enhanced abundance Lactobacillaceae, Clostridiaceae, Rikenellaceae. increased decreased Rikenellaceae respectively. IQA100 Lachnospiraceae, Leuconostocaceae, Coriobacteriaceae. altered metabolic pathways related carbohydrate lipid metabolism, amino acid biosynthesis. modulated functions, particularly cell wall synthesis, while upregulated involved energy production, secondary metabolite biosynthesis, antioxidant production. Both effective mitigating negative effects immunity, gut health microbiota broilers. Given concerns about antimicrobial resistance, are potent bacitracin, being recommended.

Language: Английский

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Effects of Dietary Bacillus subtilis BC02 Supplementation on Growth Performance, Antioxidant Capacity, and Cecal Microbes in Broilers

Agriculture, Journal Year: 2023, Volume and Issue: 13(8), P. 1561 - 1561

Published: Aug. 4, 2023

This study investigated the effects of Bacillus subtilis BC02 supplementation on broiler performance, antioxidant capacity, and cecal microbes. A total 288 one-day-old Arbor Acres broilers were randomly divided into three groups. The control group fed a basal diet, experimental groups diet supplemented with 250 500 mg/kg (BS250 BS500), respectively. results showed that increased average daily feed intake but decreased to gain ratio in from 0 14, 28, 42 days. Meanwhile, BS500 significantly triglyceride serum urea nitrogen levels compared BS250 (p < 0.05). Moreover, improved capacity by increasing jejunum superoxide dismutase glutathione, decreasing malondialdehyde mRNA expression SOD2, CAT, GPX1, Nrf2 Dietary villus height, velvet concealed ratio, expressions IL-2, IL-4, IL-10, IL-12 In addition, Bacteroidota, Proteobacteria, Helicobacter, Prevotellaceae UCG-001 0.05) group. conclusion, dietary can improve growth intestinal microflora abundances broilers.

Language: Английский

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Effect of dietary supplementation with recombinant human lysozyme on growth performance, antioxidative characteristics and intestinal health in broiler chickens

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

Lysozyme is often used as a feed additive to act an antibacterial protein that boosts the immune system of livestock and poultry while protecting against pathogens. To investigate effects recombinant human lysozyme (rhLYZ) from Pichia pastoris chlortetracycline on broiler chicken's production performance, antioxidant characteristics, intestinal microbiota, total 200, 1-d-old male Arbor Acres chickens (46.53 ± 0.42 g) were selected for 42-d experiment. Dietary treatments included basal diet corn-soybean meal supplemented with either 0 mg/kg (CON), 50 aureomycin (ANT), 20 rhLYZ (LOW), 60 (MEDIUM), or 180 (HIGH). Compared CON, MEDIUM increased (P < 0.05) average daily gain (67.40 broilers day 22 42. In early (1.29) overall phases (1.69), led reduction in conversion ratio chickens. Furthermore, comparison CON ANT, exhibited reduced levels INF-γ tumor necrosis factor-α serum. cecum, abundance Monoglobus Family_XIII_AD3011_group was lower treatment compared CON. Overall, supplementation improved growth nutrient utilization efficiency, serum function, also influencing composition microbiota. This suggests lysozyme's potential replace antibiotic additives feed.

Language: Английский

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Impact of Liposomal Hesperetin in Broilers: Prospects for Improving Performance, Antioxidant Potential, Immunity, and Resistance against Listeria monocytogenes

Avian Pathology, Journal Year: 2024, Volume and Issue: 54(1), P. 120 - 148

Published: Aug. 22, 2024

Liposomal encapsulated phytogenics, such as liposomal hesperetin, are considered novel substitutes for antibiotics in the broilers industry owing to their improved nutritional and therapeutic properties. Therefore, our key goal was investigate hesperetin impact on broilers' growth performance, health, antioxidant status, tight junction proteins (TJP), resistance against Listeria monocytogenes. Four groups were fed 0, 150, 250, 400 mg/kg of hesperetin-supplemented diets experimentally infected with L. monocytogenes strain. Herein, especially at higher concentrations, augmented FCR upregulation genes encoding TJP (occludin, JAM-2, MUC-2), attributes (GPX-1, SOD-1, CAT, HO-1, NQO1, COX2), which reflect enhancing health welfare broilers. Muscle biomarkers enhanced; meanwhile, muscle MDA, ROS, H2O2 levels reduced response hesperetin. fortification loads its virulence-related expression (flaA, hlyA, ami). Remarkably, histopathological alterations intestinal brain tissues restored post-inclusion reflects increasing bird's infection. Transcription cytokines/chemokines (MyD88, AVBD6, CCL20, IL-1β, IL-18), autophagy (Bcl-2, LC3, AMPK, AKT, CHOP, Bip, p62, XBP1) ameliorated following dietary fortification, suggests birds' immunity health. Collectively, research recommends application broilers` promoting immunity, besides antibacterial, antivirulence characteristics fight

Language: Английский

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Effects of Dietary Galla Chinensis Tannin Supplementation on Antioxidant Capacity and Intestinal Microbiota Composition in Broilers

Agriculture, Journal Year: 2023, Volume and Issue: 13(9), P. 1780 - 1780

Published: Sept. 8, 2023

The current study aimed to investigate the effects of dietary Galla Chinensis tannin (GCT) supplementation on antioxidant capacity and gut microbiota composition in broilers. Two hundred eighty-eight Arbor Acres broiler chicks were divided into CON group GCT group; each treatment contained 6 replicates with 24 per replicate for a period 42 days, fed either basal diet or supplemented 300 mg/kg GCT. Results revealed that significantly increased glutathione peroxidase (GSH-Px) activity (p < 0.05) decreased malondialdehyde (MAD) concentrations serum GSH-Px catalase (CAT) MDA small intestine. In addition, up-regulated gene expressions nuclear factor erythroid 2-related 2 (Nrf2), heme-oxygenase 1 (HO-1), (CAT), peroxidase-1 (GPX1), NAD(P)H quinone oxidoreductase 1(NQO1). High-throughput sequencing results showed abundances Faecalibacterium Megamonas 0.05). These findings will contribute our understanding intestinal

Language: Английский

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Effects of dietary supplementation with microencapsulated Galla chinensis tannins on growth performance, antioxidant capacity, and lipid metabolism of young broiler chickens

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: Oct. 26, 2023

This study aimed to investigate the impacts of dietary supplementation with Galla chinensis tannins (GCT) on growth performance, antioxidant capacity, and lipid metabolism young broilers. Overall, a total 216 healthy 1 day-old broilers were randomly allocated CON group GCT group, provided basal diet or added 300 mg/kg microencapsulated GCT, respectively, in 21 days trial. Our findings indicated that addition had no significant effects ( p &gt; 0.05) performance. However, led reduction cholesterol (TC) concentration serum liver &lt; 0.05). Furthermore, significantly increased ratios high-density lipoprotein (HDL) low-density (LDL) HDL TC serum, elevating activities enzymes related Dietary also improved capacity broilers, as evidenced by decrease malondialdehyde Additionally, exhibited expressions hepatic genes associated HO-1 , GPX1 SOD2 SIRT1 CPT-1 PPARα ) 0.05), while mRNA expression SREBP-1 was decreased compared group. In conclusion, status without affecting their

Language: Английский

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