Effects of microalgae as dietary supplement on palatability, digestibility, fecal metabolites, and microbiota in healthy dogs

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: Sept. 27, 2023

The current trend of dog owners increasingly favoring the functional value food to assure preventive health and wellbeing their pets has been raising interest in microalgae as natural additives with bioactive properties. However, scientific studies addressing effects supplementation diets for dogs are scarce. This study aimed evaluate dietary three species ( Chlorella vulgaris , Nannochloropsis oceanica Tetradesmus obliquus ) on diet palatability, total tract digestibility, metabolizable energy content, fecal metabolites microbiota dogs. Twelve adult Beagle were used two-bowl tests compare palatability a commercial complete without (reference diet) 1.5% each microalgae. From results obtained, digestibility trials performed according replicated Latin square 3 × 3, six dogs, experimental periods 10 days each, levels (0.5, 1.0, 1.5%). In trial, evaluated. First approached or tasted was not significantly affected by inclusion, but showed preference reference over inclusion C. N. no difference being observed T. . all trials, up did greatly chemical composition kept unaffected intake, output metabolites, nutrients energy. Compared diet, increased protein digestibility. Fecal characteristics supplementation, dependent species. fed microalgae-supplemented modified promoting beneficial Turicibacter Peptococcus genera associated gut activation immune system. Overall, support sustainable supplements that potentially enhance gastrointestinal through selective stimulation detrimental intake

Language: Английский

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Gut Probiotics and Health of Dogs and Cats: Benefits, Applications, and Underlying Mechanisms

Microorganisms, Journal Year: 2023, Volume and Issue: 11(10), P. 2452 - 2452

Published: Sept. 29, 2023

Pets (mostly domestic dogs and cats) play an important role in the daily lives of humans their health has attracted growing attention from pet owners. The intestinal microbiota, a complex microbial community with barrier-protective, nutritional, metabolic, immunological functions, is integral to host health. Dysbiosis been related variety diseases animals. Probiotics have used functional foods dietary supplements modulate microbiota promote health, which introduced cats recent years. Various canine- feline-derived probiotic strains isolated characterized. administration probiotics shown positive effects on gut can alleviate some disorders cats, although underlying mechanisms are largely unresolved. In this review, we summarize current knowledge benefits discuss possible order provide new insights for further development application pets.

Language: Английский

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Composition of the fecal, vaginal and colostrum microbiotas of dams at parturition and their relationship with neonatal outcomes in dogs

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: March 6, 2025

Microbial seeding in early life is critical for the host's short- and long-term health, mother first source of bacteria newborn. The objective this study was to characterize maternal fecal, vaginal, colostral microbiotas canine species one day after parturition evaluate relationship between microbial profiles 36 dams neonatal outcomes 284 newborns. part revealed presence 2 3 clusters on basis core microbiota dams. Among these three microbiotas, only vaginal microbiome found be associated with outcomes. Compared those other clusters, females Cluster 1, lowest stillbirth mortality ratios, presented a greater abundance Moraxellaceae their microbiota; 2, Pasteurellaceae, mostly from Haemophilus genus; (with highest ratios), Enterobacteriaceae, E. coli. Moreover, significantly lower richness according Shannon index than did clusters. This underscores strong association microbiota, particularly newborn health. results call further research gain deeper understanding optimal composition ways modulate it improve

Language: Английский

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Molecular biological characteristics of the intestinal microbiocenosis of dogs on a mixed diet

International Journal of Veterinary Medicine, Journal Year: 2025, Volume and Issue: 4, P. 59 - 71

Published: Jan. 16, 2025

Metabarcoding research of the domestic animal intestines is relevant for veterinary medicine. The effect feeding and food additives on its gut microbiome both applied fundamental significance demonstrates stimulation various groups bacteria development. objective our work to study complete pet feed Dilly microbial community dogs’ in breeding kennel Irkutsk. composition was determined 12 animals different ages having used Illumina MiSeq amplicon sequencing V3-V4 region 16S rRNA. Rates diversity had been adapted evaluate affluence prominence Bacterial operational taxonomic units nonparametric Mann-Whitney criteria (correlative test, p≤0,05) certainty value difference unit percentage physiological groups. All all, 147150 rRNA gene fragment sequences were obtained. dominant turned out be phyla Firmicutes, Actinobacteriota, Bacteroidota, Proteobacteria. that under almost no Fusobacteriota - markers a diet with predominance raw meat (predation). Firmicutes phylum included from five families. most numerous families among Lactobacillaceae bacteria. Representatives seven observed Actinobacteriota bacteria, which Bifidobacteriaceae predominated. Proteobacteria Enterobacterales order Burkholderiales made up small proportion entire depended age. result could perspective enlarge knowledge about digestive process an omnivorous wolf pack. These have more or less flexible microbiome, has several variants normal composition. There predominated lactic acid communities intestines, consumed contained coarse dietary fibers. In turn, reduced number Fusobacteriota.

Language: Английский

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Metagenomic analyses of gut microbiome composition and function with age in a wild bird; little change, except increased transposase gene abundance

ISME Communications, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 1, 2025

Abstract Studies on wild animals, mostly undertaken using 16S metabarcoding, have yielded ambiguous evidence regarding changes in the gut microbiome (GM) with age and senescence. Furthermore, variation GM function has rarely been studied such populations, despite metabolic characteristics potentially being associated host senescent declines. Here, we used 7 years of repeated sampling individuals shotgun metagenomic sequencing to investigate taxonomic functional Seychelles warblers (Acrocephalus sechellensis) age. Our results suggest that species richness declines terminal year, this decline occurring consistently across all ages. Taxonomic composition also shifted However, identified occurred linearly (or even mainly during early prior onset senescence species) little accelerated change later life or their year. Therefore, are not linked Interestingly, found a significant increase abundance group transposase genes age, which may accumulate passively due increased transposition induced as result stressors arise These findings reveal but senescence, vertebrate provide blueprint for future studies

Language: Английский

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Evaluation of extended-spectrum β-lactamase producing bacteria in feces of shelter dogs as a biomarker for altered gut microbial taxa and functional profiles

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 24, 2025

Background The USA is home to 83–88 million dogs, with 3–7 living in shelters. Shelter dogs move through the supply chain from their geographical origin adoptive homes, possible exposure pathogens and shift gut microbiota. However, research this area limited. This study examined effects of intestinal colonization by ESBL bacteria on taxa abundance, diversity, functions 52 shelter various ages, sexes, fertility statuses. Methodology We isolated fecal DNA, sequenced 16S, processed sequences using DADA2, identified profiles each dog Phyloseq, analyzed Chao1, Shannon, Simpson alpha diversity ggplot2 Wilcoxon test. beta Bray–Curtis dissimilarity matrix vegan package. Differential abundance taxa, microbiome functions, differential were DESeq2, PICRUSt2, ALDEx2, respectively, rank Kruskal-Wallis tests for comparisons between groups. Results Firmicutes (69.3%), Bacteroidota (13.5%), Actinobacteriota (6.77%), Proteobacteria (5.54%), Fusobacteriota (4.75%) major phyla dogs. colonized had reduced microbiota than non-colonized levels following (Proteobacteria, Deferribacterota, Bacteroidota, Fusobacteriota, Spirochaetota), class (Gammaproteobacteria, Bacteroidia, Deferribacteres, Brachyspirae, Fusobacteria), families ( Enterobacteriaceae , Peptostreptococcaceae Lactobacillaceae Lachnospiraceae Prevotellaceae ) significantly p < 0.05) varied two Further stratified analysis age, sex, spaying/neutering status influenced indicating these covariates act as effect modifiers. Most metabolic biosynthetic pathways downregulated compared alpha-linolenic acid metabolism shigellosis, fluorobenzoate degradation, allantoin toluene glycol fatty beta-oxidation, glyoxylate bypass increased bacteria. Conclusion Colonization marks altered Dog’s demography modify alterations, host factors interplay shape or other reprogram down upregulating multiple biosynthesis promote colonization.

Language: Английский

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The Microbiome in Critical Illness

Veterinary Clinics of North America Small Animal Practice, Journal Year: 2025, Volume and Issue: 55(3), P. 443 - 458

Published: May 1, 2025

Language: Английский

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Environmental risk factors in puppies and kittens for developing chronic disorders in adulthood: A call for research on developmental programming

Frontiers in Veterinary Science, Journal Year: 2022, Volume and Issue: 9

Published: Dec. 23, 2022

Many dogs and cats are affected by chronic diseases that significantly impact their health welfare relationships with humans. Some of these can be challenging to treat, a better understanding early-life risk factors for occurring in adulthood is key improving preventive veterinary care husbandry practices. This article reviews obesity enteropathy, behavioral problems, which also intractable life-changing consequences. Aspects early life puppies kittens the adult disorders include maternal nutrition, establishment gut microbiome, behavior, weaning, nutrition during growth, growth rate, socialization conspecifics humans, rehoming neutering. Despite evidence some species reviewed here reflect developmental origins disease (DOHaD), programming has rarely been studied cats. Priorities strategies increase knowledge DOHaD discussed. Critical windows development proposed: preconception, gestation, suckling period, pre-neutering or pre-puberty, post-neutering post-puberty size, durations depend upon breed. Challenges research large number breeds wide genetic phenotypic variability, existence many mixed-breed individuals. Moreover, difficulties conducting prospective lifelong cohort studies exacerbated discontinuity pet between breeders subsequent owners, dispersed nature ownership.

Language: Английский

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Age-associated changes in intestinal health biomarkers in dogs

Frontiers in Veterinary Science, Journal Year: 2023, Volume and Issue: 10

Published: Aug. 22, 2023

The gut microbiome is critical for maintaining host health. In healthy humans, the aging process one of main factors modulating changes in intestinal microbiota. However, little known about relationship between health, microbiota, and dogs. present study aims to explore differences microbiota health based on fecal biomarkers a population dogs different ages. involved 106 breeds aged 0.2 15 years categorized as senior (>7 years; n = 40), adult (2–7 50), junior (< 2 16). Fecal samples were collected during same period at facilities. analysis included following indicators: 16S rRNA gene sequencing investigate microbiota; qPCR determine dysbiosis index; short-chain fatty acid concentrations; calprotectin; immunoglobulin A. Beta diversity revealed significant difference with small effect size ( p 0.003; R 0.087) among age categories unweighted UniFrac metric, but no significance was observed weighted metric or Bray–Curtis distances. There alpha measures index categories. Senior had significantly higher relative abundance proportions phyla Bacteroidota Pseudomonadota genus Faecalibacterium , not analysis. At family level, Ruminococcaceae Uncl. Clostridiales .1 Veillonellaceae, Prevotellaceae, Succinivibrionaceae Bacteroidaceae abundances category than and/or Relative proportions, concentrations acetate, category, while butyrate, isovaleric acid, valeric lower. concentration lower category. Calprotectin A levels did differ across groups. conclusion, this multiple minor composition amount acids groups, studies larger populations representative all ages are warranted refine results.

Language: Английский

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The Canine Gut Health: Impact of a New Feed Supplement on Microbiota Composition

Published: March 29, 2024

This study aimed to determine the impact of a novel formulation supplement composed natural ingredients: bromelain, quercetin, and Lentinula edodes, on gut microbiota healthy adult dogs. Adult female dogs were administered either placebo (CTR, n = 15) or (TRT, n=15) over 28 days. Stool samples collected for 16S rRNA sequencing before administration (T0), at completion (T28), one week after end (T35) characterize changes in microbial communities. QIIME was used both alpha- beta-diversity ANCOM-BC identify differences taxonomic abundances supplementation. We found significant decrease overall diversity CTR group, but no TRT group time. Furthermore, we abundance several taxa groups beneficial bacteria more pronounced group. Specifically, increases sequences belonging genera Bifidobacterium, Lactobacillus, Pediococcus T28 with Bifidobacterium Lactobacillus persisting T35 when compared T0. Importantly, members these are known be pro anti-inflammatory associated The results our show potential selectively enhance specific bacterial taxa, offering targeted approach modulating microbiome without causing disruptions equilibrium.

Language: Английский

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