Wastewater surveillance of open drains for mapping the trajectory and succession of SARS-CoV-2 lineages in 23 cities of Maharashtra State (India) during June 2022 to May 2023

Heliyon, Journal Year: 2025, Volume and Issue: 11(4), P. e42534 - e42534

Published: Feb. 1, 2025

The timely detection of SARS-CoV-2 is crucial for controlling its spread, especially in areas vulnerable to outbreaks. However, due a lack sustainable and low cost methods, early such outbreaks impacting middle-income countries (LMICs). Leveraging Wastewater-Based Epidemiology (WBE), we examined the dissemination evolution SARS CoV2 virus open drains across urban, suburban densely populated cities selected regions state Maharashtra, third largest India. In period from June 2022 May 2023, 44.89 % RNA were positive RT-qPCR wastewater samples collected regions. Whole genome sequencing revealed 22 distinct lineages, with Omicron variant, followed by XBB dominating, alongside other variants as BF, BQ, CH, BA.2.86, albeit lower frequencies. Wastewater surveillance provided insights into viral transmission, complementing clinical surveillance. Notably, our study detected emerging prior reporting, highlighting potential WBE detection. Findings underscore correlation between population density trend load. This also highlighted significance using low-cost, tool, LMICs, where adequate methods are lacking or difficult deploy accessibility.

Language: Английский

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Wastewater surveillance of open drains for mapping the trajectory and succession of SARS-CoV-2 lineages in 23 class-I cities of Maharashtra State (India) during June 2022 to May 2023

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 21, 2024

The timely detection of SARS-CoV-2 is crucial for controlling its spread, especially in areas vulnerable to outbreaks. Leveraging Wastewater-Based Epidemiology (WBE), our study examined the proliferation, dissemination, and evolution virus across urban, suburban, densely populated regions Maharashtra state, India. Over course 12-months, from June 2022 May 2023, RNA was consistently detected wastewater samples collected open drains various state. Whole genome sequencing revealed presence 22 distinct lineages, with Omicron variant dominating July September 2022, followed by XBB February April along other variants such as BF, BQ, CH, BA.2.86, albeit lower frequencies. Wastewater surveillance provided early insights into viral transmission, complementing clinical surveillance. Notably, emerging before reporting, highlighting potential WBE detection. Our findings underscore importance tracking managing spread COVID-19, limited testing. This highlights significance global infectious disease under low middle-income countries (LMICs).

Language: Английский

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Designing the Vaccine against the Omicron Variants of COVID-19 Virus as a Target by using Immunoinformatics Approach

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 8, 2024

COVID-19 vaccines have been a key determinant in humanity’s fight against the pandemic. It has observed that vaccinated individuals shown decline instances of symptoms occurring and even overall severity where they do appear. However, due to this upgraded defence hosts several variants emerged. The most recent VOCs SARS-CoV-2 these newly emerged is omicron variant. Reports shows surface glycoprotein omicron, changed with as many around 52 mutations than its preceding variant Delta. Thus, despite availability ancestral strains, their effectiveness predicted decreases each variant, necessitating more potent emerging variants. Therefore, aim was identify vaccine candidate Omicron (BA.2 XBB.1.5) by targeting stable structural proteins using immunoinformatic. Tools like, IEDB, VaxiJen v2.0, used for identification T-cell B-cell epitopes. interaction receptors validated 3D model were predicted. Promising antigenic peptides, from (BA.2) MHC class I (HLA)-A*11-01allele, exhibiting high antigenicity. study identified promising candidates immunoinformatic SARS-CoV-2.

Language: Английский

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Retrospective Sequence Data Analysis of SARS - CoV-2 in Goa using Oxford Nanopore Technologies: A District Hospital Experience

International Journal of Science and Research (IJSR), Journal Year: 2024, Volume and Issue: 13(4), P. 1835 - 1843

Published: April 5, 2024

Background: Over the past decade, with development and refinement of third -generation sequencing technologies such as Nanopore Sequencing, Whole genome Sequencing (WGS) has become accessible to smaller institutes worldwide. The recent COVID -19 Pandemic caused by SARS -CoV -2 virus demonstrated importance Genome in observing acquisition new viral mutations, proving vital monitoring evolution variants concerns their spread within population. Method: study retrospectively analyzed sequenced data 508 samples obtained after nanopore from January 2023 July cycle threshold <30. Results: It had shown that there was a surge cases observed during March, April May, which correlated recombinant 2 positive for state Goa belonging XBB 1.16 lineage. Conclusion: This highlighted challenges performing an analysis resource -limited environment artefacts associated using Midnight Primers.

Language: Английский

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Wastewater surveillance of severe acute respiratory syndrome coronavirus-2 in open drains of two Indian megacities captures evolutionary lineage transitions: a zonation approach

Environmental Science and Pollution Research, Journal Year: 2024, Volume and Issue: 31(37), P. 49670 - 49681

Published: July 30, 2024

Language: Английский

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