Unravelling Glucoraphanin and Glucoerucin Metabolism across Broccoli Sprout Development: Insights from Metabolite and Transcriptome Analysis

Plants, Journal Year: 2024, Volume and Issue: 13(6), P. 750 - 750

Published: March 7, 2024

Variations in the concentration of glucoraphanin (GRA) and glucoerucin (GER), as well corresponding breakdown products, isothiocyanates (ITCs) nitriles, were investigated during growth broccoli sprouts. The concentrations GRA GER decreased sharply from 33.66 µmol/g to 11.48 12.98 8.23 µmol/g, respectively, after seed germination. From third seventh day, both maintained relatively stable. highest sulforaphane (17.16 µmol/g) erucin (12.26 observed on first day. Hereafter, nitrile hydrolyzed or higher than those ITCs. Moreover, ratio 1.35 0.164 1 d 5 d, with a similar trend exhibited for erucin/erucin 2 d. RNA-seq analysis showed that BolMYB28 BolCYP83A1, involved aliphatic glucosinolate (GSL) biosynthesis, remained largely unexpressed until In contrast, genes operating within GSL-myrosinase hydrolysis pathway highly expressed right beginning, their expression levels increasing significantly Additionally, we identified two BolESPs six BolNSPs might play important roles promoting production nitriles development

Language: Английский

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Grafting Compatibility and Environmental Conditions on Soilless Eggplant (Solanum melongena) Grown in the Mediterranean Greenhouse

Horticulturae, Journal Year: 2023, Volume and Issue: 9(9), P. 1060 - 1060

Published: Sept. 21, 2023

Grafting techniques represent an efficient tool to enhance plant growth and development. The study aims explore the effects of different grafting combinations on eggplants under diverse greenhouse conditions. Eggplant cultivar Black bell (Bb) was employed as scion, while hybrid F1 Beaufort (Be) Solanum torvum (To) were utilized rootstock. hypothesis behind this pertains incompatibility with F1. It postulates that can be mitigated by manipulating soil temperatures. experimental factors encompassed (Bb, Be/Bb To/Bb), well substrate temperature (both cold or heated). combination showed higher values vegetative traits, but it exhibited low compatibility. Additionally, physiological analysis confirmed presence excessive disorder within combination. Examination xylem vessels revealed notable differences between involving Bb rootstock Be/Bb, compared one To/Bb non-grafted Bb. Specifically, area, diameter number approximatively 45% in than Furthermore, a robust linear correlation observed height morphometric except fruits sets. This lays basis for novel protocol agriculture, addressing rootstocks regulating air

Language: Английский

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Exploitation of a Grafting Technique for Improving the Water Use Efficiency of Eggplant (Solanum melongena L.) Grown in a Cold Greenhouse in Mediterranean Climatic Conditions

Agronomy, Journal Year: 2023, Volume and Issue: 13(11), P. 2705 - 2705

Published: Oct. 27, 2023

Grafting techniques have been intricately associated with the optimization of water use efficiency (WUE). In this study, various eggplant (Solanum melongena L.) rootstock–scion combinations were compared under three irrigation regimes (IR): 50% deficit in volume (IR50), a doubling (IR200), and normal watering (IR100). The cultivar Black Bell (Bb) was employed as scion, while rootstock adopted included F1 hybrids Energy (En) Beaufort (Be) one accession S. torvum (To). trial encompassed evaluation no- self-grafted plants. Plants grown cold greenhouse Sicily assessed for their morphological parameters, well fruit production quality. leaf analysis chromatic parameters potential. Significant variation observed plant height, exhibiting lowest values combinations. potential varied significantly relation to combination regime. Fruit quality traits displayed significant variations L* a*, fruit’s longitudinal transversal diameters soluble solid content. number fruits per combination; highest recorded grafted onto by IR50. weight interaction between experimental factors study. Notably, WUE calculated production, studied ascertained. registered IR50, specifically To/Bb. This research aims develop comprehensive water-efficient organic farming protocol sustainable agriculture.

Language: Английский

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The Phytonutrient Content and Yield of Brassica Microgreens Grown in Soilless Media with Different Seed Densities

Horticulturae, Journal Year: 2023, Volume and Issue: 9(11), P. 1218 - 1218

Published: Nov. 10, 2023

Microgreens are increasingly valued by consumers for their phytonutritional benefits. There is limited information to growers on the influence of growth media and seed density antioxidant properties Brassica microgreens. Therefore, study was conducted determine effect seedling (Hygromix, Promix, TS1) (4, 8, 12 seeds per cavity) morphological parameters, yield, color, components, activities in radish (Raphanus sativus), cabbage (Brassica oleracea), rocket (Eruca sativa) Fourteen days after seeding, Promix at a cavity improved yield radish, cabbage, Irrespective density, all three microgreens grown TS1 had higher leaf nitrogen, phosphorus, calcium content. Interaction effects Hygromix x densities were more pronounced (DPPH FRAP). These differences could be due physical medium. Vitamin C total glucosinolate 4 cavity. Twelve medium whereas phytochemicals most likely growing media, followed mineral content low

Language: Английский

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Different myrosinases activate sequestered glucosinolates in larvae and adults of the horseradish flea beetle

Insect Biochemistry and Molecular Biology, Journal Year: 2023, Volume and Issue: 163, P. 104040 - 104040

Published: Nov. 22, 2023

β-Glucosidases play an important role in the chemical defense of many insects by hydrolyzing and thereby activating glucosylated pro-toxins that are either synthesized de novo or sequestered from insect's diet. The horseradish flea beetle, Phyllotreta armoraciae, sequesters pro-toxic glucosinolates its brassicaceous host plants possesses endogenous β-thioglucosidase enzymes, known as myrosinases, for glucosinolate activation. Here, we identify three myrosinase genes P. armoraciae (PaMyr) with distinct expression patterns during beetle ontogeny. By using RNA interference, demonstrate PaMyr1 is responsible activity adults, whereas PaMyr2 larvae. Compared to PaMyr2, PaMyr3 was only weakly expressed our laboratory population, but may contribute Silencing resulted lower larval survival a predation experiment also reduced breakdown uninjured This suggests involved both activated turnover In assays recombinant preferred different substrates, which consistent enzyme activities crude protein extracts adults larvae, respectively. These differences were unexpected because larvae sequester same glucosinolates. Possible reasons discussed.

Language: Английский

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A Cold Case—Glucosinolate Levels in Kale Cultivars Are Differently Influenced by Cold Temperatures

Horticulturae, Journal Year: 2023, Volume and Issue: 9(9), P. 953 - 953

Published: Aug. 22, 2023

Among the Brassica oleracea L. crops, kale has gained increased global recognition in recent years as a healthy food item due to its high nutritional value and versatility. Additionally, diversity of different varieties started be explored across large latitudes from Mediterranean north temperate climates. Specifically, glucosinolates are predominant phytochemicals found leaves, contributing specific taste this vegetable, they affected by environmental factors such temperature. To date, no study investigated effect chilling on glucosinolate and, thus, genetically cultivars at same time. Given variability observed among cultivars, we evaluated impact acclimation cold temperatures levels curly kale, Lacinato feral type using high-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-ESI-qTOF-MS). We targeted short-term (after 12 h) well longer-term seven days) acclimation. Our results revealed molecular patterns regarding change compared Lacinato-type kale. In latter ones, primary aliphatic were induced (the glucoraphanin more than 200%). The indole glucobrassicin was not significantly affected. Conversely, reduced 35% after acclimation, whereas hardly indicate that both genetic important for composition conclusion, obtain plants an improved value, considering temperature choice cultivar is crucial during cultivation. Future breeding attempts should also emphasize cultivar-dependent reported here.

Language: Английский

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New tools for organic farming: amino acids and Trichoderma spp. application improved snap bean (Phaseolus vulgaris L.) seed yield and quality

Acta Horticulturae, Journal Year: 2024, Volume and Issue: 1391, P. 693 - 700

Published: March 1, 2024

Language: Английский

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Light Use Efficiency of broccoli (Brassica oleracea var. italica Plenck) and rocket (Eruca sativa L.) during the initial plant growth stages

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 336, P. 113408 - 113408

Published: June 16, 2024

Language: Английский

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Glucosinolates and Their Hydrolytic Products—A Love Story of Environmental, Biological, and Chemical Conditions

Journal of AOAC International, Journal Year: 2024, Volume and Issue: 107(5), P. 867 - 875

Published: June 24, 2024

Abstract Background Glucosinolates (GSL) play an important role in providing defense to plants and helping them cope with various biotic, as well abiotic, stresses. Many living beings including humans animals, some herbivores, have adapted themselves use this mechanism for their own use. More than 120 glucosinolates are distributed within a large number of plants. factors known influence the GSL composition plant. Among these, cofactors, myrosinase isozymes, heavy metals environmental conditions such light, CO2 temperature regulation. These ensure that different glucosinolate compositions can be produced by plants, thus impacting mechanism. Objective The objective current review is highlight importance responsible affecting concentration. Methods has been compiled using accessible literature from Pubmed, Scopus, Google scholar. Efforts made restrict last 5 years (2018–2023), exceptions. Results critical acts resource all researchers working on these essential compounds. It provides information may production. also gives opportunity modify plant given information. Conclusions long ignored class biomolecule. plethora biological activities compounds useful. Though there harmful components goitrin progoitrin, easily removed modulating highlighted review. Highlights covered most ability mechanistic action discussed available literature.

Language: Английский

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Characterisation and causal model of the holistic dynamics of the integral sustainability of the agri-food system

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(6), P. e0305743 - e0305743

Published: June 27, 2024

The transformation of the food and industrial agricultural production system into adaptative sustainable systems capable being productive within social, environmental, economic limits is a crucial factor in reducing risk to security growth. However, analysis structure effect these variables environments remains unknown, whereby technology processes are considered as equivalent critical level those already described. purpose this study design model that enables characterisation agri-food sector based on determination from integral systemic approach. Tools, such viable model, employed analyse dynamics generate balanced scorecard, which items learning continuous improvement added. Lastly, impact principles sustainability versus variation revealed, useful determining appropriate levels guarantee balance foundations circularity. From approach, can be adopted by agronomists scientists alternative strategies for management sustainability.

Language: Английский

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